견의 Toxoplasmosis에 관한 연구

이청산,곽학구,박경재,장환,Lee, Cheong-San,Kwak, Hak-Koo,Park, Kyung-Jae,Jang, Hwan 한국동물위생학회 1998 韓國家畜衛生學會誌 Vol.21 No.1

This study was carried out to investigate the prevalence of antibody against Toxoplasma gondii in the dog by Latex agglutination test and Indirect fluorescent antibody test. Two month-old dogs were infected intraperitoneally with T gondii to observe histopathological and immunohisto-chemical changes. Results obtained through this experiment were summarized as follows ; 1. Among the serum samples of 163 heads of the dog, 10 samples(6.1%) were positive. 2. In the sex, 6 heads (7.1%) out of 84 female dogs and 4 heads(5.1%) out of 79 male dogs were positive. However, there were no significant differences between the male and female. 3. Overall proportion of agreement between indirect fluorescent antibody and Latex agglutination test in 163 sera of dogs was 97.5%. 4. When 2 month-old dogs were infected intraperitoneally with T gondii, main clinical signs were intermittent fever, dyspnea, diarrhea. In general, the infected dogs recovered closely on the 11th day of post-inoculation. 5. At necropsy, petechial and ecchymotic hemorrages and swelling in small intestine, lung, spleen, liver and kidney were observed. 6. In histopathological observation, interstitial pneumonia, hyperemia and hemorrhages in lung were observed. Focal necrosis of hepatocytes, the neutrophil and basophil in the renal tubular epithelium were observed. 7. By immunohistochemical staining using Vectorstain ABC kit, the positive cells were recognized in the lung and the liver. 8. By indirect fluorescent antibody test, the Toxoplasma antibodies in the infected dogs were detected on the 15th day of postinoculation.

꽃사슴의 Clostridium perfringens A형에 의한 장독혈증 발생보고

이청산 ( Cheong San Lee ),한성태 ( Sung Tae Han ),곽학구 ( Hak Koo Kwak ),박경재 ( Kyung Jae Park ),현공율 ( Gong Yul Hyun ),조우영 ( Woo Young Cho ),이종인 ( Jong In Lee ),배유찬 ( You Chan Bae ),진영화 ( Yong Hwa Jean ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.2

The case reports for clostridium perfringens type A enterotoxemia in Formosan deer have rarely been reported. This paper describes a natural case of type A enterotoxemia in farmed Formosan deer in Cheongwon-gun. A dead, male 10-month-old Formosan deer was submitted to Chungbuk Livestock and Veterinary Research Institute, March 24, 2001 and examined. That deer was fed with assorted grain feed, oak leaves, acorn and bean curd. Grossly there was no visible external change. Despite of the carcass being examined within 12 hours of death, there was a quite degree of postmortem decomposition. There was severe hemorrhage in the serosa of abomasum and small intestine. Much blood tinged and watery contents were contained in those organs. Also there were severe swelling of spleen, some red foci in hepatic parenchyma. Microscopically there were severe congestion and hemorrhage in mucosa, submucosa, muscular layer, and serosa of abomasum and small intestine. Also spleen and pancreas showed severe congestion and hemorrhage. There were multifocal hemorrhage with hepatic necrosis in periportal area and focal mononuclear cell deposition in sinusoid. In bacterial culture for small intestine, Cl peifringens was isolated. By toxin typing for the strain, that had α-toxin belonged to type A. In electronmicroscopy for feces, no virus particle was detected. Considering clinical signs, gross lesions, microscopic lesions, bacterial culture, and toxin typing of the isolate, this case was diagnosed as enterotoxemia by Cl perfringens type A.

국내 분리 닭 전염성 기관지염 바이러스 성상에 관한 연구

이청산 ( Cheong San Lee ),조우영 ( Woo Young Cho ),최윤식 ( Yun Sic Choi ),김순재 ( Soon Jae Kim ) 한국가축위생학회 1991 韓國家畜衛生學會誌 Vol.14 No.1

In order to investigate the biological properties, pathogenicity and immune responses in artficially infected SPF chickens with Avian infectious bronchitis virus that was isolated from chickens showing IB like signs in southern region of Chung buk. Results obtained throuth the experiments are summarized as follows. 1. From 15 IB suspected cases, two strains of IB virus were isolated, one each from the tracheas and lungs. 2. Infectious bronchitis specific embryo lesions were observed after four serial passages of the isolates in chicken embryos. 3. The field isolates and M-41 strain of IB virus interfered with the replication of Newcastle disease virus in chicken embryos. 4. When specific pathogen free chickens, two week old, were inoculated with the TB virus isolates, clinical respiratory signs as dyspnea, coughing were observed. Airsacculitis was observed by necropsy. 5. AGP antibody positive rates of inoculated SPF chickens were highest on day 14 and lowest on day 36, while HI antibody responses were detected on day 14 in all Groups, the reinoculated Group was shown highest titers. 6. By Indirect immunofluorescence antibody assay of artificially infected SPF chickens, the viral antigens were detected in tissues of larynx, trachea and lung on the 4th to 7th days post inoculation.

도축돈의 호흡기질병에 관한 연구

이은정 ( Eun Jeong Lee ),박경재 ( Kyung Jae Park ),이청산 ( Cheong San Lee ),손현수 ( Hyun Soo Son ),김원설 ( Won Seoul Kim ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.3

A survey on pneumonic lungs and its histopathological changes of the slaughtered pigs from the southern area of Chungbuk province was carried out during the period from January to December 1999. Pneumonic lungs were attempted bacteriological findings and antibiotic susceptibilities. The results obtained were as follows; 1. Of 158 slaughtered pigs, 97(61.4%) pigs had pneumonic lesions in the lung, and the prevalence was high in winter, spring, autumn, and summer in order. 2. The bacteria isolated from pneumonic lesions were pasteurella spp, 13 heads(34.2 %), streptococcus spp, 6(15.8 %), actinobacillus spp, 3(7.9 %), coliform 4(10.5 %) and the other bacteria, 12(31.6 %). 3. These isolates were highly susceptible to the antibiotics of enrofloxacin 30(78.9 %), cephalothin 28(73.6 %) and ceftiofur 27(71 %). 4. Histopathologically, swine enzootic pneumonia and pleuropneumonia lesions were observed. The swine enzootic pneumonia lesions were consisted of peribronchiolar lymphoid hyperplasia and exudate in alveolar lumen. The pleuropneumonia lesions were consisted of thrombosis, alveolar wall thickened by mononuclear cells and neutrophil deposition.

혈구응집억제반응과 효소면역측정법을 이용한 닭 뉴캣슬병 바이러스에 대한 혈중항체가 비교

한성태 ( Sung Tae Han ),이청산 ( Cheong San Lee ),곽학구 ( Hak Koo Kwak ),송종한 ( Jong Han Song ),이종인 ( Jong In Lee ) 한국가축위생학회 2003 韓國家畜衛生學會誌 Vol.26 No.3

This study was conducted to investigate the similarity between hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay(ELISA) titers and sample to positive ratio (S/P ratio) of Newcastle disease(ND) virus. To perform this study, the 372 sera of broiler chicks and 120 sera of layers and breed chicks were collected from slaughter house and farms, respectively. As a result of Hi test out of different chicks, the positive percentage of ND antibody titer of broiler, layer and breeder, when a standard positive HI titer were `2`, was 84.4%, 100% and 100%, respectively. The positive percentage of ND antibody titer by ELISA was shown 38.4%, 100% and 100% and S/P ratio were also shown 81.5%, 98.2% and 99.2%, respectively. The results of comparative survey with same sera by two experimental methods were as follows; In low HI titer, ELISA titer was not similar to HI titer, but S/P ratio was similar to it. In high HI titer, ELISA titer and S/P ratio were similar to HI titer. Therefore, HI titer was more similar to S/P ratio than ELISA titer.

ELISA를 이용한 우결핵검사 결과에 대한 PPD 접종법 결과 분석 비교

이종진 ( Jong Jin Lee ),김덕순 ( Duk Soon Kim ),이종화 ( Jong Hwa Lee ),이청산 ( Cheong San Lee ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.4

On the basis of the 2009 business plan, 20,394 Korean native cattle and beef cattle were carried examination of bovine tuberculosis by using ELISA technique from March to December. As a result, 66 cattle tested positive for tuberculosis and showed 0.32% positive ratio. Intradermal tuberculin test about 66 cases of ELISA positive cattle was carried out, and all of 66 cattle were confirmed as negative. However, when 7 PPD-positive cattle derived from slaughterhouse were tested by 20k ELISA kit and MS ELISA kit, 3 (2 suspect) cattle and 5 cattle showed positive results, respectively. As compared to the results of PPD test, the concordance rates were 43% (71% included suspect) with 20k ELISA kit and 71% with MS ELISA kit.

면역조직화학염색 및 전자현미경검사를 이용한 소 유두로부터 소유두종바이러스 검출

배유찬,윤순식,박중원,이청산,진영화,강문일,Bae, You-Chan,Yoon, Soon-Seek,Park, Jung-Won,Lee, Cheong-San,Jean, Yong-Hwa,Kang, Mun-Il 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.2

We examined the teats of slaughtered cattles to investigate the prevalence of papilloma and to detect bovine papilloma virus (BPV) from those samples. Among 880 heads, 432 (49.0%) was Holstein and 448 (51.0%) was Korean native cattle. Grossly, out of 432 heads (Holstein), 263 (60.8%) had papilloma lesions on teats. However, out of 448 heads (Korean native cattle), only 33 (7.4%) had papilloma lesions on teats. Immunohistochemically, BPV antigen was detected in 35 heads (22.9%) of 153 (Holstein) which had papilloma lesions. But, in Korean native cattle, BPV antigen was detected in 1 head (3.8%) of 26. Electronmicroscopically, in Holstein papilloma cases, BPV particles was detected in 31 heads (39.2%) of 79. This study revealed that papilloma was very prevalent in holstein teats, Korea and the prevalence of papilloma in Holstein was 8 times higher than that of Korean native cattle. Moreover, it was needed to consider gross and histopathological lesions to confirm BPV infection on teats because the sensitivity of immunohistochemistry and electronmicroscopy was not high.

견(犬)의 Toxoplasmosis에 관한 연구

장환 ( Hwan Jang ),박경재 ( Kyung Jae Park ),이청산 ( Cheong San Lee ),곽학구 ( Hak Koo Kwak ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.1

This study was carried out to investigate the prevalence of antibody against Toxoplasma gondii in the dog by Latex agglutination test and Indirect fluorescent antibody test. Two month-old dogs were infected intraperitoneally with T gondii to observe histopathological and immunohisto-chemical changes. Results obtained through this experiment were summarized as follows; 1. Among the serum samples of 163 heads of the dog, 10 samples(6.1%) were positive. 2. In the sex, 6 heads (7.1%) out of 84 female dogs and 4 heads(5.1%) out of 79 male dogs were positive. However, there were no significant differences between the male and female. 3. Overall proportion of agreement between indirect fluorescent antibody and Latex agglutination test in 163 sera of dogs was 97.5%. 4. When 2 month-old dogs were infected intraperitoneally with T gondii, main clinical signs were intermittent fever, dyspnea, diarrhea. In general, the infected dogs recovered closely on the 11th day of post-inoculation. 5. At necropsy, petechial and ecchymotic hemorrages and swelling in small intestine, lung, spleen, liver and kidney were observed. 6. In histopathological observation, interstitial pneumonia, hyperemia and hemorrhages in lung were observed. Focal necrosis of hepatocytes, the neutrophil and basophil in the renal tubular epithelium were observed. 7. By immunohistochemical staining using Vectorstain ABC kit, the positive cells were recognized in the lung and the liver. 8. By indirect fluorescent antibody test, the Toxoplasma antibodies in the infected dogs were detected on the 15th day of postinoculation.

간접형광항체법을 이용한 담수양식어의 병원균 Edwardsiella tarda의 검출

류해진 ( Hae Jin Ryu ),조우영 ( Woo Young Cho ),이청산 ( Cheong San Lee ),허강준 ( Gang Joon Heo ) 한국가축위생학회 1993 韓國家畜衛生學會誌 Vol.16 No.2

In this study, we carried out the rapid diagnostic system based on indirect fluorescent antibody technique(IFAT) for detection of bacterial diseases in cultured freshwater fishes. 1. When the fishes were tested with graded dilution of Edwardsiella tarda FPC 470 bacteria detection from ten fishes injected with 4.1×10(3) colony forming unit(CFU)/ml, all of them were detected by IFAT but only two fishes were recognizable by the culture method in the tested fishes injected with 4.1×10(3) CFU/ml. 2. The bacteria E.tarda could be detected by IFAT method from 1 to 48hrs after injection in the tissues tested such as kidney, liver and spleen of the fishes, whereas detection by culture method could be recognized from 1 to 48hrs after injection in the kidney and spleen but it was not possible from preinjection to 1 hr in the liver. 3. Thus, IFAT proved to be more useful technique than plate culture method in the diagnosis of Edwardsiellosis in the freshwater fishes.

2008년 국내 산양유 시료에서의 세균 및 체세포 수 조사

남향미 ( Hyang Mi Nam ),임숙경 ( Suk Kyung Lim ),김종만 ( Jong Man Kim ),장금찬 ( Geum Chan Jang ),정석찬 ( Suk Chan Jung ),위성환 ( Sung Hwan Wee ),한홍율 ( Hong Ryul Han ),이청산 ( Cheong San Lee ) 한국예방수의학회(구 한국수의공중보건학회) 2011 예방수의학회지 Vol.35 No.1

A total of 222 udder-half milk samples of lactating goats were collected from two herds in Korea during 2008 and all samples were subjected to bacteriological examination. Somatic cell counts (SCC) were also determined for all samples except for 13 (5.9%), which were collected from halves of udders with clinical mastitis. A total of 85 bacteria were isolated from 82 (36.9%) of 222 milk samples tested. Staphylococci were the predominant pathogens, accounting for almost 70% of the isolates: Coagulase negative staphylococci (CNS) and S. aureus constituted 55% (47/85) and 14.1% (12/85), respectively. Among 209 samples tested for SCC, bacteria were isolated from 36 of 115 (31.3%) samples with SCC of <1×106 cells/㎖and 38 of 94 (40.4%) samples that had SCC of ≥1×106 cells/㎖, respectively. All S. aureus were detected from samples with SCC of ≥1×106 cells/㎖, while 25 of 47 (61.0%) CNS were isolated from milk samples with SCC of <1×106 cells/㎖. Mean SCC of milk samples that harbored S. aureus and CNS was 4,787×103 cells/㎖and >1×106 cells/㎖, respectively. All S. aureus and CNS isolates were susceptible to all antimicrobials tested except for penicillin, to which 2 (16.6%) S. aureus and 12 (25.5%) CNS isolates showed resistance.