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류해진 ( Hae Jin Ryu ),장쾌식 ( Que Sik Jang ),김중규 ( Joong Kew Kim ),김영태 ( Young Tae Kim ),한석기 ( Seok Gi Han ),김영욱 ( Young Uk Kim ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.1
The dermatophytes was isolated from skin of the 175 healthy dogs and 22 dogs with pathological skin lesions at Pohang and Kyong.ju. The isolates were identified by the morphological appearance after cultivation and lactophenol cotton blue staining. 1. The isolation rates of dermatophytes were 8.5%(15/175) in dog with healthy skin and 27°/c (6/22) in dogs with pathological skin lesions. 2. From asymptomatic dogs, the isolation rates of dermatophytes in female dogs were higher than those in male dogs and those in young dogs were higher than those in old dogs. 3. Isolation rates of microsporum canis and trichophyton spp from asymtomatic dogs were 14 (93%) and 1(6.7%), respectively. 4. The causative agents of 6 dogs with dermatophytosis were identified as all M ccnis.
간접형광항체법을 이용한 담수양식어의 병원균 Edwardsiella tarda의 검출
류해진 ( Hae Jin Ryu ),조우영 ( Woo Young Cho ),이청산 ( Cheong San Lee ),허강준 ( Gang Joon Heo ) 한국가축위생학회 1993 韓國家畜衛生學會誌 Vol.16 No.2
In this study, we carried out the rapid diagnostic system based on indirect fluorescent antibody technique(IFAT) for detection of bacterial diseases in cultured freshwater fishes. 1. When the fishes were tested with graded dilution of Edwardsiella tarda FPC 470 bacteria detection from ten fishes injected with 4.1×10(3) colony forming unit(CFU)/ml, all of them were detected by IFAT but only two fishes were recognizable by the culture method in the tested fishes injected with 4.1×10(3) CFU/ml. 2. The bacteria E.tarda could be detected by IFAT method from 1 to 48hrs after injection in the tissues tested such as kidney, liver and spleen of the fishes, whereas detection by culture method could be recognized from 1 to 48hrs after injection in the kidney and spleen but it was not possible from preinjection to 1 hr in the liver. 3. Thus, IFAT proved to be more useful technique than plate culture method in the diagnosis of Edwardsiellosis in the freshwater fishes.