$H_{2}O_2$와 A$\beta$로 유도된 pc12 cell에서 생산조인(生酸棗仁) 수추출물의 항산화 및 항치매 효과

이상원,김대현,윤종현,김진우,정은영,이성근,이기상,김태헌,류영수,강형원,Lee, Sang-Won,Kim, Dae-Hyun,Yun, Jong-Hyun,Kim, Jin-Woo,Jung, Ejun-Young,Lee, Seoung-Geun,Lee, Key-Sang,Kim, Tae-Heon,Lyu, Yeoung-Su,Kang, Hyung-Won 대한한방신경정신과학회 2008 동의신경정신과학회지 Vol.19 No.3

Objective: The antioxidant and anti-Alzheimer effects of Semen Ziziphi Spinosae (SZS) water extract against the amyloid beta peptide (1-42) or H202-induced oxidative damage and cell death were investigated in rat pheochromocytoma line PC 12. Methods: The cells were incubated with SZS water extract and oxidative damage-inducing materials, amyloid beta peptide (1-42) or H2O2 for 24 h. The cellular viability was assessed by WST-1 assay, cytotoxic damage by LDH activity assay, oxidative damages of cells by fluorescence spectrophotometric method, and apoptosis by TUNEL staining assay. Results and Conclusions: 1. Preincubation of the cells with SZS water extract prior to amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) exposure elevated the cell survival close to the control and decreased the level of LDH activity and the fluorescence from the cell homogenates and TUNEL staining of the cells, compared to only amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) treated conditions. 2. Our study suggests that Semen Ziziphi Spinosae (SZS) water extract has protective effects against amyloid beta peptide (1-42) or H2O2-induced cell toxicity through the antioxidation mechanism, which might be beneficial for the treatment of Alzheimer's disease.

레버 증폭 구조의 플렉서를 이용한 공초점 현미경의 개발

이상원,김위한,정영대,박민규,김지현,이상인,이호,Lee, Sang-Won,Kim, Wi-Han,Jung, Young-Dae,Park, Min-Kyu,Kim, Jee-Hyun,Lee, Sang-In,Lee, Ho 한국광학회 2011 한국광학회지 Vol.22 No.1

본 연구에서는 레버 증폭 구조를 이용한 플렉서 기반의 나노 스테이지를 설계, 제작하였다. 제작된 나노 스테이지를 샘플 스테이지로 채용한 공초점현미경을 개발하였다. 2차원 이미징의 구현을 위해서, 기존의 공초점현미경은 레이저를 이미징 평면상에 스캐닝하는 방식으로 구현된다. 본 연구에서는 백 나노미터의 구동정밀도를 가지는 나노 스테이지 위에 놓인 샘플을 2차원으로 스캐닝하면서 2차원 이미지를 구현할 수 있는 공초점현미경을 개발하였다. 플렉서 기반의 나노 스테이지는 이중 판스프링, 변위증폭 레버, PZT 엑추에이터 그리고 변위 센서로 구성 되어 있다. 스테이지의 구동 성능 해석을 위해 상용 유한요소 해석 프로그램을 이용하였다. 현미경에 사용되는 광원은 적색광 레이저이며, 레이저는 여러 광학요소를 거쳐 샘플스테이지의 샘플에 입사되고, 반사된 빛은 광센서인 PMT(Photo Multiplying Tube)로 계측되게 된다. 계측된 빛의 크기를 이용하여서 2차원 이미징을 구현하였다. 개발된 공초점 현미경으로 생쥐 귀의 피부조직을 관찰하여 현미경의 이미징 성능을 검증하였다. 설계된 샘플 스테이지는 기존의 공초점 현미경의 기계적인 Beam 스캐너를 대신함으로써 현미경의 광경로 및 전체시스템을 간소화 하였다. A confocal microscope was developed utilizing a scanning sample stage based on a home-built double-compound flexure guide. A scanning sample stage with nano-scale resolution consisted of a double leaf spring based flexure, a displacement amplifying lever, a Piezo-electric Transducer(PZT) actuator and capacitance sensors. The performance of the two-axis stage was analyzed using a commercial finite element method program prior to the implementation. A single line laser was employed as the light source along with the Photo Multiplier Tube(PMT) that served as the detector. The performance of the developed confocal microscope was evaluated with a mouse ear skin imaging test. The designed scanning stage enabled us to build the confocal microscope without the two optical scanning mirror modules that are essential in the conventional laser scanning confocal microscope. The elimination of the scanning mirror modules makes the optical design of the confocal microscope simpler and more compact than the conventional system.

회원칼럼 I - 국민생명, 건축법에 맡길 수 없다.

이상원,Lee, Sang-Won 한국건축구조기술사회 2010 건축구조 Vol.17 No.2

"동의보감"과 "제중신편"에 기재된 처방중 수치대황의 종류와 효능에 대한 고찰

이상원,김현정,김진숙,Lee, Sang-Won,Kim, Hyeun-Jeong,Kim, Jin-Sook 한국한의학연구원 2003 한국한의학연구원논문집 Vol.9 No.1

The Effects and processing methods of Rhei Rhizoma from Donguibogam and Chejungsinpyeon were studied, Through the study, we found 21 kinds of processing method of Rhei Rhizoma in Donguibogam and Chejungsinpyeon, Processing methods used in Donguibogam and Chejungsinpyeon were effective from reducing side effects of Rhei Rhizoma, and were safe in clinical application, the aims of processing methods of Rhie Rhizoma were safer application by reducing poisonous character, and increase the range of clinical application by useing 21 kinds of processing methods.

다발성 요추간반 탈출증에서 추간반조영술의 진단적 의의

이상원,김긍년,진동규,김영수,조용은,진병호,Lee, Sang-Won,Kim, Keung-Nyun,Chin, Dong-Kyu,Kim, Young-Soo,Cho, Yong-Eun,Jin, Byung Ho 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.5

Objective : The introduction of MRI makes it easy to detect multiple lumbar disc herniation. However, MRI is not a physiologic test for detecting the symptomatic level. For the surgical plan, it is very important to determine the symtomatic level among the multiple lumbar disc herniation. In this regard, we studied diagnostic significance of discography on multiple lumbar disc herniation in determining the symptomatic level. Method and Material : We retrospectvely analyzed the discographic and clinical findings of 121 patients with multiple lumbar disc herniation for investigating the diagnostic availability of discography. All were surgically treated from January 1995 through May 1998. Result : Discography provocated the same pain as usual symptom in 99 out of 121 patients(81.8%). Compared with surgical findings, the diagnostic accuracy of the discography in multiple lumbar disc herniation was 75.6%, sensitivity was 64.6%, and specificity 87.2%. There was no correlation between the pain provocation of discography and the extent of annular degeneration on CT/discogram. The pain provocation showed good correlation with the extent of annular disruption on CT/discogram. The rate of same result(correlation rate) between the discography and D.I.T.I was 81.4% in multiple lumbar disc herniation patients with unilateral leg pain. Conclusion : These results indicate that in multiple lumbar disc herniation, the discography is considered useful diagnostic tool to determine the symptomatic level and to decide the surgical plan.

퍼지집합 질적 비교분석을 통한 의료분야 사용자 혁신 단계별 핵심요인 조합 연구

이상원,신준석,Lee, Sang-Won,Shin, Juneseuk 기술경영경제학회 2016 Journal of Technology Innovation Vol.24 No.4

본 연구는 의료분야 사용자 혁신을 아이디어 도출-연구개발-상용화 및 창업의 세 단계로 분류하고, 각 단계별로 혁신의 성공에 미치는 핵심요인의 조합을 30개 국내 사례에 대한 퍼지집합 질적 비교분석을 통해 파악했다. 아이디어 단계에서는 사용자 인적 네트워크상에서 그 가치를 평가하고, 연구개발 단계로의 이행을 위한 자원을 제공해 주는 것이 중요했다. 연구개발 단계에서는 급진적 혁신은 기술역량, 자원 접근성, 정부지원이 모두 갖추어져 있거나 우수한 혁신 네트워크에 아이디어가 도입된 경우 발생했다. 상용화/창업의 경우 급진적 혁신은 연구개발과 같이 세 조건이 모두 갖춰진 경우 발생했으며, 점진적 혁신은 기술역량이 탁월한 개인이 소규모 정부지원 또는 주변 자원을 활용해 이루어졌다. 민간기업의 사용자 혁신 전략과, 정부 지원 정책의 효과, 효율성을 모두 제고할 수 있는 사용자 혁신의 틀을 제공했다는 점에 현실적 의미가 있다. 학술적으로는 사용자 혁신 단계별 핵심요인 조합을 규명하고, 퍼지집합 질적 비교분석방법을 적용한 첫 연구라는 의의가 있다. We examine how combinations of key factors affecting healthcare user innovation vary by innovation stages from idea generation through R&D to commercialization and venturing using a fuzzy-set Qualitative Comparative Analysis (fsQCA) of thirty Korean cases in the healthcare field. Our empirical analysis shows that well-functioning innovation network and easy resource acquisition facilitate ideation of radical user innovation. However, technological capability and governmental support are crucial to make a shift to R&D as well as commercialization stages. Differently, incremental user innovation depends heavily on technological capability of users. Our analysis can provide policy makers as well as corporate innovation mangers with a strategic framework for boosting user innovation along three stages.

반도체 공정용 플라즈마 발생장치의 종류와 특성

이상원,Lee Sang-Won 한국전기전자재료학회 2006 전기전자재료 Vol.19 No.6

고등학교(高等學校) 학생(學生)의 대수(代數) 문제(問題) 해결(解決) : 전략(戰略)과 오류(誤謬) 분석(分析)

이상원,전평국,Lee, Sang-Won,Jeon, Pyung-Kook 한국수학교육학회 1997 수학교육논문집 Vol.2 No.-

The purpose of this study is to provide the primary sources to improve the problem solving performance by analyzing the errors and the strategies selection of the high school students when solving given algebraic problems. To attain the purpose of this study, the questions for investigation in this study are : 1. What are the differences / similarities in the patterns of errors committed by successful and unsuccessful problem-solvers when solving particular algebraic problems ? 2. What are the error types chosen by unsuccessful problem-solvers when solving particular algebraic problems? 3. Do students utilize checking, either locally or globally, when solving particular algebraic problems? Twenty students were drawn out of 10th grade students in J girls' high school in Yengi -gun, Chung-Nam, for this study. The problem-solving test was used as a test instrument. From the data, the verbal protocols and the written protocols were analyzed by the patterns. The conclusions drawn from the results obtained in the present study are as follows: First, in solving particular algebraic problems, when the problems were solved with one strategy, most students didn't give any consideration to other strategies. So mathematics teachers should teach them to use the various strategies, and should develop the problems to be used the various strategies. Second, in solving particular algebraic problems, errors on notions or transformations of equations were found. Thus, the basic knowledges related to equation should be taught. In addition, most unsuccessful students seleted the strategies inadequately to solve the problems because of misunderstanding the problems. So, to improve the problem solving performance the processes of 'understanding problem' should be emphasized to students. Third, although the unsuccesful students used the 'checking' processes when solving the problems, most of them did not find the errors because of misconceptions related to the problems, carelessness, and unskillfulness of checking. Thus, students must be taught more carefully and encouraged to use the checking.

경제적 관점에서 본 4대강 살리기 사업

이상원,Lee, Sang-Won 한국수자원학회 2009 물과 미래(한국수자원학회지) Vol.42 No.1

Design of Health Warning Model on the Basis of CRM by use of Health Big Data

이상원,신성윤,Lee, Sangwon,Shin, Seong-Yoon The Korea Institute of Information and Commucation 2016 한국정보통신학회논문지 Vol.20 No.8

Lots of costs threaten the sustainability of the national health-guarantee system. Despite research by the national center for disease control and prevention on health care dynamics with its auditing systems, there are still restrictions of time limitation, sample limitation, and, target diseases limitation. Against this backdrop, using huge volume of total data, many technologies could be fully adopted to the preliminary forecasting and its target-disease expanding of health. With structured data from the national health insurance and unstructured data from the social network service, we attempted to design a model to predict disease. The model can enhance national health and maximize social benefit by providing a health warning service. Also, the model can reduce the advent increase of national health cost and predict timely disease occurrence based on Big Data analysis. We researched related medical prediction cases and performed an experiment with a pilot project so as to verify the proposed model. 오늘날 많은 비용이 국가 의료보장체계의 유지를 위협하고 있다. 국가 질병 통제 및 방지 센터의 감사체계를 동반한 건강관리 역학성에 대한 연구에도 불구하고, 시간 한계, 표본 한계, 대상 질병 한계에 대한 제약이 여전히 존재하고 있다. 이러한 배경에서, 방대한 양의 전수 데이터를 활용하여, 많은 기술들이 건강의 선제적 예측이나 그 대상 질병을 확장하는 분야에 충분하게 적용되고 있다. 우리는 국민건강보험의 구조적 데이터와 소셜네트워크서비스의 비구조적 데이터를 활용하여 질병을 예측하는 모형을 설계하였다. 이 모형은 건강예보서비스를 제공함으로써, 국민건강을 증진시키고 사회적 혜택을 극대화할 수 있다. 또한, 빅데이터 분석에 근거하여, 건강보험비용의 갑작스러운 증가를 감소시키거나 적시적인 질병발생을 예측할 수도 있다. 관련된 의료 예측 사례를 살펴보았고, 제안된 모형의 검증을 위하여 시범과제를 통한 실험을 수행하였다.