강우 및 지반조건에 따른 토양침식 특성

이명구,송창섭,Lee, Myung-Gu,Song, Chang-Seob 한국농공학회 2011 한국농공학회논문집 Vol.53 No.3

This study is analyzed the characteristics of the soil erosion with the geotechnical conditions and rainfall conditions, such as the ground slope, the compaction ratio, rainfall intensity and duration of rainfall etc. To this ends, a series of model test are conducted on clayey sands. From the results, the variation of soil loss is analyzed with the geotechnical and the rainfall conditions. The amount of soil loss is decreased as the increase of compaction ratio and is increased as the ground slope, rainfall intensity and the duration of rainfall.

Protoberberine 화합물이 P815 세포중의 serotonin 함량에 미치는 영향

이명구,김응일,허재두,이경순,노재섭,Lee, Myung-Koo,Kim, Eung-Il,Hur, Jae-Doo,Lee, Kyong-Soon,Ro, Jai-Seup 한국생약학회 2001 생약학회지 Vol.32 No.1

The effects of protoberberine compounds on serotonin biosynthesis in P815 cells were investigated. Protoberberine compounds such as berberine, palmatine and coralyne decreased serotonin content dose-dependently, but coptisine did not. The $IC_{50}$ values of berberine, palmatine and coralyne were $3.0\;{\mu}M,\;16.5\;{\mu}M\;and\;14.5\;{\mu}M$, respectively. Protoberberine compounds at concentrations up to $20\;{\mu}M$ were not cytotoxic towards P815 cells. The activity of tryptophan hydroxylase, a ratelimiting enzyme in serotonin biosynthesis, was inhibited by the exposure of berberine, palmatine and coralyne in P815 cells (14.9-19.3% inhibition at $2-15\;{\mu}M$), but that of aromatic L-amino acid decarboxylase was not. These results suggest that the inhibition of tryptophan hydroxylase activity by berberine, palmatine and coralyne might partially contribute to the decrease in serotonin content in P815 cells.

이형상 칼슘 인산염(Biphasic Calcium Phosphate)에 의한 성체 줄기세포의 골아세포 분화

이명구,임성우,피성희,김윤상,신형식,유형근,Lee, Myoung-Ku,Lim, Sung-Woo,Pi, Sung-Hee,Kim, Yun-Sang,Shin, Hyung-Shik,You, Hyoung-Keun 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.4

The present study was to determine the influence of micro-macro biphasic calcium phosphate(MBCP) on proliferation and differentiation of human marrow-derived mesenchymal stem cells. Primary stem cells were cultured from bone marrow and 3-4 passaged cells were used. This study tested the proliferative effects by cell counting. Collagen sythensis, alkaline phosphatase activity, expression of osteocalcin and bone sialoprotein by Western blot analysis were evaluated. The cellular proliferation of ASC was not influenced by MBCP. Collagen synthesis of ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The ALP activity in ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The expression of OC and BSP incresaed in ASC cultured on MBCP. These results suggest that MBCP may stimulates the osteoblastic activity of ASC.

아토피피부염 온라인 커뮤니티를 통해 본 환자들의 경향과 한의학적 치료이용률을 높이기 위한 방안 연구

이명구,김상현,김안나,장현철,Lee, Myung-Ku,Kim, Sang-Hyun,Kim, An-Na,Jang, Hyun-Chul 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.4

Objectives : The purpose of this study is to understanding the patients thought of Korean Medical treatment and to suggest an improvement in utilization of Korean Medicine treatment about atopic dermatitis. Methods : We had investigate atopic dermatitis online community by classifying the post by treatment type, feature type of the information and by content to know the patients latest tendency. And then compare the result to understand the patients thought of Korean Medical treatment. Results : Most of the post was experiential information and about 67% of the post was about symptom and treatment. Especially there had lot of interest about there treatment information and self-treatment. By contrast Korean Medical treatment type has lower rate then other type of treatment about symptom and treatment and the information was not specific. This result may influence on utilization of Korean Medical treatment. Conclusions : To solve this problem patients needs easy access to Korean Medical treatment and more information about there treatment.

수종의 생약제제가 human fetal osteoblasts의 염기성 인산분해 효소 활성에 미치는 영향

이명구,최희인,유형근,신형식,Lee, Myoung-Ku,Choi, Hee-In,You, Hyung-Keun,Shin, Hyung-Shik 대한치주과학회 2003 Journal of Periodontal & Implant Science Vol.33 No.1

Several growth factors and polypeptides are not commonly yet used for regenerators of bone tissue or alveolar bone because of the insufficiency of studies on their side effects, genetic engineering for mass production and stability for clinical application. Recently, many herbal medicines, which have advantage of less side effects and possibility of long-term use, have been studied for their capacity and effects of anti-bacterial, antiinflammatory and regenerative potential of periodontal tissues. Morindae Radix, Cibotium Barometz (L.), Albizziae Cortex, Cistandhis Herba have been traditionally used as medicines for treatment of bone disease in Eastern medicine. The objective of the present study is to examine the ability of alkaline phosphatase (ALP) activity of human fetal osteoblast (hFOB1) when several natural medicines were supplemented. hFOB1 were cultured with Dulbecuo's Modified Eagle's Medium Nutrient Mixture F-12 HAM ( DMEM/F-12 1:1 Mixture, Sigma, USA) and negative control, dexamethasone (positive control), and each natural medicines for 3 days. And then ALP activity was measured by spectrophotometer for enzyme activity and Alizarin red S staining for morphometry. Among the natural medicines of this study, Morindae Radix, Cibotium Barometz (L.) and Cistanchis Herba induced higher activity of ALP synthesis than negative controls in all experimental group. Albizziae Cortex showed mild increases than negative control group. According to measurement of positively stained area, all of the natural medicines of this study increased compared to negative control. Especially, Cibotium Barometz (L.) and Cistanchis Herba showed statistical significance compared to negative control (p<0.05). These results indicate that Morindae Radix, Cibotium Barometz (L.), Albizziae Cortex, Cistandhis Herba have an inducing ability of ALP synthesis on osteoblast.

자율 주행차를 위한 딥러닝 기반 주행 가능 영역 검출

윤동휘(Dongwee Yoon),이명희(Myeonghee Lee),백주열(Juyoul Back),김인중(Injung kim) 한국정보과학회 2020 한국정보과학회 학술발표논문집 Vol.2020 No.12

바이오매스 유래의 저해물질이 에탄올 생산에 미치는 영향

이명구(Myunggu Lee),조대행(Dae Haeng Cho),김용환(Yong Hwan Kim),이진원(Jinwon Lee),이종호(Jong Ho Lee),김승욱(Seung Wook Kim),조재훈(Jaehoon Cho),김상용(Sangyong Kim),박철환(Chulhwan Park) 한국생물공학회 2009 KSBB Journal Vol.24 No.5

목질계 바이오매스 전처리시 발생하는 저해물질로써 퓨란류, 유기산류, 페놀류를 포함한 발효에 있어, S. cerevisiae K35와 P. stipitis KCCM 12009가 에탄올 생산에 미치는 영향을 중심으로 연구를 수행하였다. 본 연구에 사용된 두 균주 모두 24시간 내에 100 g/L의 glucose를 모두 소비하였으며, 40 g/L 이상의 에탄올을 생산하였다. 저해물질로 퓨란 (5-HMF, furfural)류가 1-2 g/L 존재시, S. cerevisiae K35는 furfural에 비해 5-HMF에 의한 저해영향을 높았으며, P. stipitis KCCM 12009는 그와 반대의 경향을 보였다. Acetate가 5 g/L 이상 존재하는 경우, S. cerevisiae K35와 P. stipitis KCCM 12009 두 균주 모두에 균체량 및 에탄올 생산에 대한 저해정도가 가장 높았다. 또한, 페놀류 (syringaldehyde, vanillic acid, syringic acid)가 각각 1-2 g/L 존재시, S. cerevisiae K35는 비교적 적은 세포성장 저해영향과 소량 증가하는 에탄올 생산량을 보인 반면, P.stipitis KCCM 12009는 전반적으로 S. cerevisiae K35의 세포성장과 에탄올 생산에 있어 유사한 경향을 보였으나, syringaldehyde와 vanillic acid가 2 g/L 존재시에는 24시간에 균생장이 급격히 감소함과 동시에 에탄올 생산량도 감소함을 보였고, 48시간 배양 후 저해물질의 영향에서 벗어나 정상적인 세포성장과 에탄올을 생산하는 경향을 보였다. S.cerevisiae K35와 P. stipitis KCCM 12009에 동일한 농도의 저해물질이 존재시 저해영향의 차이를 보이는 것은 균주 자체의 특성 차이로 인한 저해물질에 대한 민감도를 나타내는 것으로 보인다. 본 연구를 통해 두 균주를 이용한 바이오 에탄올 생산시 발생되는 저해물질의 농도별 선택적 특이성을 확인할 수 있었으며, 이를 통해 목질계 바이오매스 전처리시 발생되는 저해물질의 적정농도를 확인할 수 있었다. The process for ethanol production requires lignocellulosic biomass to be hydrolyzed to generate monomeric sugars for the fermentation. During hydrolysis step, a monomeric sugars and a broad range of inhibitory compounds (furan derivatives, weak acids, phenolics) are formed and released. In this study, we investigated the effects of inhibitory compounds on the fermentative performance of Saccharomyces cerevisiae K35 and Pichia stipitis KCCM 12009 in ethanol production, two yeast strains were fermented in the synthetic medium including six inhibitory compounds such as 5-hydroxymethylfurfura (5-HMF), furfural, acetic acid, syringaldehyde, vanillic acid and syringic acid. Ethanol of over 40 g/L was produced by two yeast strains in the absence of inhibitory compounds, respectively. Most inhibitory compounds except acetic acid had a little effect on the ethanol production, but acetic acid showed high inhibition effect on the cell growth and ethanol production.

음식물 침출수 청정화를 위한 파일롯 규모의 생물-분리막 복합공정의 성능 평가 연구

이명구(Myunggu Lee),박철환(Chulhwan Park),이도훈(Dohoon Lee),김탁현(Tak-Hyun Kim),이병환(Byunghwan Lee),이진원(Jinwon Lee),김상용(Sangyong Kim) 한국생물공학회 2008 KSBB Journal Vol.23 No.1

Seed Mash와 유산균 발효액을 첨가한 밀가루 반죽의 물성학적 특성

이명구(Myung-Ku Lee),이정훈(Jeong Hoon Lee),이시경(Si-Kyung Lee) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.3

Koji를 배양한 seed mash, 유산균, 효모 등으로 발효하여 제조한 발효액종을 밀가루에 첨가하여 파리노그래프, 익스텐소그래프, 아밀로그래프, 대용량 배합기로 반죽의 물리적 특성 등을 분석하였다. 파리노그래프에서 발효액종의 종류에 관계없이 흡수율은 동일하였으나 발효액종 첨가로 반죽 발전시간이 길어졌고, 안정도는 감소하였다. 익스텐소그래프에서 반죽저항성은 발효액종 첨가로 증가하였고, R/E값도 증가하여 seed mash, S. cerervisiae, L. brevis로 발효시킨 발효액종을 첨가한 반죽에서 가장 높았다. 아밀로그래프에서 호화온도와 최고점도 온도는 대조구와 시험구간에 차이가 거의 없었으나 최고점도는 발효액종 첨가 시 낮아졌다. 반죽의 물리적 특성에서 D.V.(development value)값은 대조구에 비하여 모두 낮아졌고, 반죽발전시간도 짧아졌다. This study was carried out to determine the rheological properties of bread doughs containing flour-ferments prepared with seed mash obtained by Koji incubation, yeast, and lactic acid bacteria, using farinograph, extensograph, amylograph, and large scale-dough mixer. According to farinograph, the addition of the flour-ferments did not influence the water-absorption rate of doughs, regardless of the kinds of flour-ferments, however, it increased development time and decreased stability of doughs. According to extensograph, both dough resistance and resistance-to-extensibility ratio increased with the addition of flour-ferments. Especially the dough containing the flour-ferments prepared with seed mash, S. cerervisiae, and L. brevis showed the highest resistance-to-extensibility ratio. According to amylograph, although the doughs containing the flour-ferments did not show the differences in gelatinization temperature and temperature at maximum viscosity with the control which does not contain the flour-ferments, they showed lower maximum viscosity than the control. They also showed lower development value and faster development time.

액체 이산화탄소 조건에서의 캐놀라 오일 유래의 효소적 바이오디젤 생산

이명구(Myunggu Lee),박철환(Chulhwan Park),조재훈(Jaehoon Cho),이준학(Junhak Lee),이도훈(Dohoon Lee),김상용(Sangyong Kim) 한국생물공학회 2010 KSBB Journal Vol.25 No.4

액체 이산화탄소를 반응용매로 사용하여 메탄올에 의한 효소 활성이 저해되는 것을 방지하고 친환경적이며 에너지 절감 효과가 우수한 바이오디젤 생산 방법을 제시하고자 유기용매(t-butanol, acetone, chloroform, hexane, THF, cyclohexane, toluene)와 액체 이산화탄소 비교실험을 통해 액체 이산화탄소가 기타 유기용매와 같은 메탄올 저해를 방지하는 역할을 함으로써 반응 용매로서의 적합함을 확인하였다. 또한 동일 이산화탄소를 이용한 초임계 이산화탄소와 비교 실험을 수행하여 초임계 이산화탄소를 이용한 바이오디젤 생산과 유사한 결과를 나타냄으로써 액체 이산화탄소를 이용한 바이오디젤 생산방법이 에너지절약형 친환경 바이오디젤 생산에 더욱 적합함을 확인할 수 있었다. 그리고 액체 이산화탄소 상태에서 바이오디젤 생산의 최적화를 통해 고효율 효소는 novozym 435, lipozyme RM IM 및 lipozyme TL IM 중에서 1,3-위치 선택적 특이성을 가진 lipozyme TL IM이 가장 우수함을 확인할 수 있었다. 또한 경제적 최적 조건과 고전환율 최적조건을 도출하여 각각 88.3%, 99.7%의 높은 전환율을 얻었다. 본 연구를 통해 액체 이산화탄소가 메탄올에 의한 효소활성 저해를 방지하는 반응 용매로 적합하며, 지구 온난화의 주요 요인인 이산화탄소를 이용할 뿐 아니라 비독성 용매로서 친환경적이며 초임계 이산화탄소 상태보다 에너지 절감효과가 우수하여 효소적 바이오디젤 생산 방법에 새로운 방안을 제시 할 수 있었다. It has been well known that organic solvents like t-butanol and n-hexane can protect lipases from the inhibition by short-chain alcohols in the enzymatic transesterification. However, use of the organic solvents should be minimized considering their negative effects on environment and human health. Therefore, use of the greener solvents has been pursued in various are as including the enzymatic biotranformation. In this study, the liquid carbon dioxide (LCO₂) was employed as an alternative media for the enzymatic transesterification of canola oil. The conversion in the LCO₂ was comparable with those in organic solvents and the supercritical carbon dioxide, and under optimum conditions, the value reached 99.7%. It is expected that this method can provide a new type of biodiesel production process with higher energy efficiency and lower environmental impact.