Plasma Secretin Concentrations in Fasting and Postprandial States of Normal Korean Subjects

심여림,조양혁,심상수,남상채,김명석,Sim, Yeo-Rim,Jo, Yang-Hyeok,Sim, Sang-Soo,Nam, Sang-Chae,Kim, Myung-Suk The Korean Physiological Society 1985 대한생리학회지 Vol.19 No.2

한국의 정상 성인 20 명에서 공복시 및 음식물 섭취후의 철장 secretin농도를 방사면역 측정법(radioimmunoassay)으로 다음과 같이 측정하였다. 20명중 12명은 단백질 음식물인 햄버거와 쌀밥을 일주일 간격으로 섭취하였다. 햄버거 섭취 추 평균 혈장 secretin농도$(12{\sim}16\;pg/ml)$는 공복시의 평균 혈장 secretin농도(10 pg/m1 이하) 보다 유의하게 증가하였으며 그 증가가 오랫동안 지속하였다. 쌀밥의 섭취 후에는 혈장 secretin농도$(9{\sim}13\;pg/ml)$가 공복시의 값보다 증가하는 경향이었으며, 단지 식후 30분의 값에서만 유의하게 증가하였다. 식후 혈장 secretin농도의 증가폭은 햄버거에서의 증가 값이 쌀밥에서의 값보다 더 컸다. 나머지 8명은 자당 용액과 생리 식염수를 마셨다. 자당 용액의 섭취후 평균 혈장 secretin농도$(10{\sim}14\;pg/ml)$는 공복시의 값보다 유의하게 증가하였으나 쌀밥의 값과 마찬가지로 일시적이었다. 그러나 생리 식염수를 섭취한 후에는 혈장 secretin농도에 이렇다할 변화가 없었다. 이상의 결과로 미루어 정상 한국 성인에서는 단백질 음식물은 물론 탄수화물 음식물의 섭취로도 혈장 secretin농도가 증가한다고 사료된다. This study was conducted to investigate fasting plasma secretin and postprandial secretin concentrations after ingestion of a protein meal or a sucrose solution in 20 healthy Korean subjects. In 12 subjects, ingestion of a protein meal, hamburger resulted in a significant and sustained increase in the mean plasma secretin concentrations, from mean fasting levels of less than 10 pg/ml to $12{\sim}16\;pg/ml$, and the mean plasma secretin concentrations, $9{\sim}13\;pg/ml$, after a rice meal increased significantly but transiently compared with mean fasting levels. The magnitude of postprandial increase in the Plasma secretin concentration after the hamburger was greater than that of the rice meal. In the remaining 8 subjects, drinking of a sucrose solution resulted also in a significant but transient increase in the mean Plasma secretin concentrations, from mean fasting levels of less than 10 pg/ml to $10{\sim}14\;pg/ml$ which were significantly greater than that after a physiological saline. Significant increase in the plasma secretin concentration was not observed during the postprandial period after the physiological saline. It is inferred from the above results that the Plasma secretin levels increase significantly after ingestions of a carbohydrate meal as well as a protein meal in the Korean race.

C6세포에서 phospholipase A2촬성에 대한 ATP의 작용

심상수(Sang Soo Sim),김명준(Myung June Kim),윤신희(Shin Hee Yoon),김창종(Chang Jong Kim),조양혁(Yang Hyeok Jo) 대한약학회 2001 약학회지 Vol.45 No.4

To investigate action of ATP on ischemia-induced brain injury we measured phospholipase A2 activity and nitric oxide (NO) production in C6 cells. ATP alone did not have any influence on phospholipase A2 activity but increased NO production. Glutamate (1 Mm) significantly increased phospholipase A2 activity whereas did not increased NO production. ATP significantly inhibited phospholipase A2 activation induced by 0.1μM A23187, 1 Mm glutamate and 1 Mm H2O2, but did not inhibited 1mM PMA-induced phospholipase A2 activation. From the above results, it is suggested that the action of ATP in C6 cells has dual actions, such as the inhibition of agonist-induced phospholipase A2 activation and the increase of NO production.

정맥주입한 알콜이 횐쥐의 췌장 외분비에 미치는 영향

심상수(Sang Soo Sim),김창종(Chang Jong Kim) 大韓藥學會 2002 약학회지 Vol.46 No.3

To investigate the effect of intravenous ethanol administration on pancreatic exocrine secretion, we measured volume and protein amount in pancreatic juice and assayed amylase activity and phospholipase A 2 activity in pancreatic fragments and serum. Acute pancreatitis Induced by obstruction of common bile-pancreatic duct (CBPD) and caerulein infusion (57g7g1hr) showed typical characteristics, such as hyperamylasemia and pancreatic edema and increase of phospholipase A 2 activity in pancreatic fragments and serum. Intravenous ethanol infusion (50 mgHg/hr) significantly stimulated pancreatic exocrine secretion, but such a stimulatory effect of ethanol disappeared at dose of 100 mg/kg/hr without typical symptoms of acute pancreatitis. In microscopic examination, there were no typical changes of edematous pancreatitis in ethanol administrated rats. These results suggest that acute ethanol administration has dual effect on exocrine pancreatic secretion: low dose of ethanol (50 mg/hg/hr) stimulates pancreatic exocrine secretion, whereas high dose of ethanol (100 mghg/hr) does not without typical changes of edematous pancreatitis.

KCl과 phenylephrine에 의한 대동맥 수축에서 Ca2+ 길항제와 protein kinase 억제제들의 비교 효과

심상수(Sang Soo Sim),문성원(Sung Won Moon),이윤혜(Yun Hye Lee),이정근(Jung Keun Lee),김현준(Hyun Jun Kim),박진형(Jin Hyoung Park),이준한(June Han Lee),조중형(Jung Hyung Cho),김창종(Chang Jong Kim) 대한약학회 1999 약학회지 Vol.43 No.5

To investigate the difference of contractile mechanism between KCl and phenylephrine-induced contraction, we observed effects of Ca2+ antagonists and protein kinase inhibitors on aorta contraction of rats. Verapamil dose-dependently inhibited the contraction induced by KCl and pheylephrine, the inhibitory effect of verapamil was more potent in KCl-induced contraction than phenylephrine-induced contraction. Econazole and TMB-8 significantly inhibited KCl-induced contraction but did not inhibit phenylephrine-induced contraction. Staurosporine dose-dependently inhibited both KCl and phenylephrine-induced contraction. Genistein and calmodulin antagonists (W-7 and trifluoperazine) also inhibited both contraction in a dose dependent manner. However, the inhibitory effects of genistein and calmodulin antagonists were more potent in pheylephrine-induced contraction than KCl-induced contraction. These results suggest that involvements of Ca2+ channel and protein kinase in rat aorta contraction were dependent on agonist causing aort smooth muscle contraction.

정상 한국 성인의 공복시 및 식후 혈장 Secretin 농도

심여림(Sim, Yeo-Rim),조양혁(Jo, Yang-Hyeok),심상수(Sim, Sang-Soo),남상채(Nam, Sang-Chae),김명석(Kim, Myung-Suk) 대한생리학회 1985 대한생리학회지 Vol.19 No.2

한국의 정상 성인 20 명에서 공복시 및 음식물 섭취후의 철장 secretin농도를 방사면역 측정법(radioimmunoassay)으로 다음과 같이 측정하였다. 20명중 12명은 단백질 음식물인 햄버거와 쌀밥을 일주일 간격으로 섭취하였다. 햄버거 섭취 추 평균 혈장 secretin농도(12 ~ 16 pg/ml)는 공복시의 평균 혈장 secretin농도(10 pg/m1 이하) 보다 유의하게 증가하였으며 그 증가가 오랫동안 지속하였다. 쌀밥의 섭취 후에는 혈장 secretin농도(9 ~ 13 pg/ml)가 공복시의 값보다 증가하는 경향이었으며, 단지 식후 30분의 값에서만 유의하게 증가하였다. 식후 혈장 secretin농도의 증가폭은 햄버거에서의 증가 값이 쌀밥에서의 값보다 더 컸다. 나머지 8명은 자당 용액과 생리 식염수를 마셨다. 자당 용액의 섭취후 평균 혈장 secretin농도(10 ~ 14 pg/ml)는 공복시의 값보다 유의하게 증가하였으나 쌀밥의 값과 마찬가지로 일시적이었다. 그러나 생리 식염수를 섭취한 후에는 혈장 secretin농도에 이렇다할 변화가 없었다. 이상의 결과로 미루어 정상 한국 성인에서는 단백질 음식물은 물론 탄수화물 음식물의 섭취로도 혈장 secretin농도가 증가한다고 사료된다. This study was conducted to investigate fasting plasma secretin and postprandial secretin concentrations after ingestion of a protein meal or a sucrose solution in 20 healthy Korean subjects. In 12 subjects, ingestion of a protein meal, hamburger resulted in a significant and sustained increase in the mean plasma secretin concentrations, from mean fasting levels of less than 10 pg/ml to 12 ~ 16 pg/ml, and the mean plasma secretin concentrations, 9 ~ 13 pg/ml, after a rice meal increased significantly but transiently compared with mean fasting levels. The magnitude of postprandial increase in the Plasma secretin concentration after the hamburger was greater than that of the rice meal. In the remaining 8 subjects, drinking of a sucrose solution resulted also in a significant but transient increase in the mean Plasma secretin concentrations, from mean fasting levels of less than 10 pg/ml to 0 ~ 14 pg/ml which were significantly greater than that after a physiological saline. Significant increase in the plasma secretin concentration was not observed during the postprandial period after the physiological saline. It is inferred from the above results that the Plasma secretin levels increase significantly after ingestions of a carbohydrate meal as well as a protein meal in the Korean race.

고양이 위(胃)의 수축 및 전기활동에 대한 에탄올과 칼슘의 관계

김명석,심상수,윤신희,한상준,김정진,최현,Kim, Myung-Suk,Sim, Sang-Soo,Yoon, Shin-Hee,Han, Sang-Jun,Kim, Chung-Chin,Choi, Hyun 대한생리학회 1987 대한생리학회지 Vol.21 No.2

This was study carried out to investigate the effect of calcium on spontaneous contraction and electrical activity induced by ethanol in gastric smooth muscle. After peeling off the mucous membrane from the isolated whole stomach of 102 cats, two kinds of small muscle preparations $(2.0{\times}0.2\;cm)$, one longitudinal and the other circular, were excised from the fundus, the corpus and the antrum portion of each whole stomach specimen. The isometric contraction of the small muscle preparation was measured in a cylinder-shaped chamber filled with Krebs-Ringer-dextrose solution (pH 7.4, temperature $36{\pm}0.5^{\circ}C$) bubbling with 5% $CO_2$ in $O_2$. A large muscle preparation $(5.0{\times}1.2\;cm)$ was excised from the anterior wall of the corpus-antrum portion of the same specimen in 72 of 102 cats. The gastric electrical activity (slow wave and spike potential) was monopolarly recorded by four capillary electrodes (Ag-AgCl), of which two were placed on the corpus and two on the antrum, in a muscle chamber filled with the same solution as described above. Changes in the amplitude of the contraction, frequency of the gastric slow wave and the production of the spike potential were observed after adding ethanol and/or under the treatments with verapamil, $CaCl_2$ and Ca-free Krebs-Ringer-dextrose solution. The results were as follows: 1) After adding ethanol, the spontaneous phasic contraction of the corpus was reduced dose-dependently (0.125-2.0%), which was totally abolished by higher concentrations (2.0-8.0%) of ethanol. 2) The corporal phasic contraction was also completely abolished by verapamil $(3{\times}10^{-5}\;M)$ or Ca-free Krebs-Ringer-dextrose solution. The contraction was increased by $CaCl_2\;(1.8{\times}10^{-3}\;M)$, but the inhibitory effect of ethanol on the contraction persisted even under the treatment with $CaCl_2$. 3) At higher concentrations, ethanol caused tonic contraction of both preparations from the fundus, the corpus and the antrum in a dose-dependent manner. The tonic contraction of the fundus produced by ethanol was not influenced by $CaCl_2$ or verapamil, whereas the tonic contraction was not produced by ethanol in tile Ca-free solution. 4) Frequency of gastric slow wave was decreased dose-dependently by the addition of ethanol (0.25-1.0%), and tile slow wave was not produced by higher concentration of ethanol (2.0%). 5) The frequency of slow wave was significantly reduced by verapamil only and the inhibitory influence of ethanol on the slow wave frequency was reinforced by verapamil. 6) The treatment of $CaCl_2$ increased significantly the slow wave frequency, and attenuated the inhibitory effect of ethanol on the frequency. It is therefore suggested that ethanol regulates the phasic contraction and the production of slow wave by interfering with the transport of calcium in the stomach muscle of the cat.

Effect of Dopamine on Propagation and Frequency of Slow Wave in Cat Isolated Stomach Muscle

조양혁,심상수,김명석,김정진,최현,Jo, Yang-Hyeok,Sim, Sang-Soo,Kim, Myung-Suk,Kim, Chung-Chin,Choi, Hyun The Korean Physiological Society 1986 대한생리학회지 Vol.20 No.1

위(胃) 전기활동(서파)의 전파(傳播) 및 발생빈도에 미치는 dopamine의 영향을 주명하기 위하여 145마리의 고양이를 사용하며 다음의 실험을 실시하였다. 위의 복측부분을 적출하여 종주근의 주행 방향으로 대만쪽에서 길이 5cm, 폭 1.2cm인 근절편을 만들어, 95% $O_2$와 5% $CO_2$가 계속 공급되는 Krebs-Ringer용액 (PH 7.4, 온도 $36{\pm}0.5^{\circ}C$)내에 두고 가느다란 은선이 들어있는 모세관 전극 (Ag-AgCl)을 사음하여 단극성으로 서파를 기록하였다. dopamine의 첨가후 위서파의 전파방향은 첨가한 농도에 비례하여 전환이 많아졌으나, dopamine의 영향은 domperidone의 전처치로 유의하게 억제되었다. dopamine은 또한 농도가 증가함에 따라 불규칙한 위서파의 발생만도를 증가시켰으며 이 현상은 domperidone 및 Phentolamine에 의하여 억제되었으나 Propranolol, hexamethonium 및 tetrodotoxin에 의하여는 억제되지 않았다. 그러므로 dopamine은 고양이 위에서 dopamine receptor와 일부 ${\alpha}-adrenergic\;receptor$에 작용하며 이상적(異常的)서파를 발생시킨다고 사료된다. The effect of dopamine on the propagation and the frequency electrical activities (slow wave) of the stomach was studied in isolated stomach muscle strips of 145 cats, The gastric slow wave monopolarly recorded by four capillary electrodes (Ag-AgCl) in Krebs-Ringer solution $(ph\;7.4,\;temperature\;36{\pm}0.5^{\circ}C)$ bubbled with 5% $CO_2$ in $O_2$. Dopamine caused concentration-dependent changes of direction of slow wave propagation with decline in development of irregular propagation by domperidone pretreatment. Dopamine also increased the variation of slow wave frequency concentration-dependently. The variation of slow wave frequency induced by dopamine was significantly inhibited by pretreatment with domperidone and phentolamine but not with propranolol, hexamethonium and tetrodotoxin. It is therefore suggested that dopamine plays a role in the genesis of gastric electrical abnormality acting on dopamine receptors and partly on ${\alpha}-adrenergic$ receptors in cats.

Acteoside와 Isoacteoside의 항산화 작용 및 미백 효과

윤미연,심상수,황완균,최병철,Yoon, Mi-Yun,Sim, Sang-Soo,Whang, Wan-Kyun,Choi, Byung-Chul 대한약학회 2009 약학회지 Vol.53 No.1

This study was performed to investigate the effects of acteoside and isoacteoside isolated from Clerodendron trichotomum Thunberg on melanin production in B16 melanoma cells. In DPPH radical scavenging activity, acteoside and isoacteoside had a potent anti-oxidant activity in a dose-dependent manner. Both acteoside and isoacteoside dose-dependently inhibited silica-induced ROS (reactive oxygen species) generation in B16 melanoma cells. They significantly inhibited tyrosinase activity and melanin production in MSH-stimulated B16 melanoma cells. The inhibitory effect of acteoside was more potent than that of isoacteosidee. In Western blot of tyrosinase, acteoside inhibited MSH-induced tyrosinase expression in B16 melanoma cells, which is related to the inhibitory action of acteoside on tyrosinase activity and melanin production. These results show that acteoside and isoacteoside from Clerodendron trichotomum Thunberg has a potent antioxidant activity and whitening activity. The underlying mechanism of acteoside on whitening activity may be due to the inhibition of tyrosinase activity and tyrosinase expression.

바위취 에탄올 추출물의 항산화 및 항노화 작용

윤미연,임혜원,심상수,최태부,Yoon, Mi-Yun,Lim, Hye-Won,Sim, Sang-Soo,Choe, Tae-Boo 대한약학회 2007 약학회지 Vol.51 No.5

To investigate the effect of ethanol extract of Saxifraga stolonifera MEERBURGH on skin care, we measured anti-oxidant and anti-aging activity. S. stolonifera ethanol extract itself had anti-oxidant activity in a dose-dependent manner in DPPH radical scavenging. Silica dose-dependently increased the intracellular ROS generation in RAW 264.7 cells. S. stolonifera ethanol extract inhibited silica-induced intracellular superoxide anion generation, $H_2O_2$ and hydroperoxide generation in RAW 264.7 cells. S. stolonifera ethanol extract significantly inhibited both hyaluronidase and elastase activity, also significantly inhibited MMP-1(collagenase) activity as well. In NIH 3T3 fibroblast cells, S. stolonifera ethanol extract significantly increased collagen-like polymer synthesis, which suggesting the S. stolonifera ethanol extract might be used as hydration and anti-wrinkle agents. From the above results, it is suggested that the main ingredients of S. stolonifera ethanol extract play an important role in anti-oxidant and anti-aging activity.

B16 Melanoma 세포에서 Arctigenin이 Tyrosinase 활성과 Melanin 생성에 미치는 영향

이동자(Dong Ja Lee),심상수(Sang Soo Sim) 대한약학회 2012 약학회지 Vol.56 No.6

To investigate the possibility of development as a whitening agent using arctigenin, we measured DPPH assay, NBT/XO assay, intracellular ROS scavenging assay, tyrosinase assay and MSH-induced melanin production in B16 melanoma cells. Arctigenin dose-dependently had anti-oxidant activity in DPPH, NBT/XO and intracellular ROS assay. Although arctigenin did not inhibit purified tyrosinase activity, it dose-dependently inhibited tyrosinase activity and melanin production in B16 melanoma cells stimulated by 1 μM α-MSH. In particular, arctigenin at a concentration 100 μM inhibited α-MSHstimulated tyrosinase activity and melanin production by 50.9±2.9% and 69.0±6.5% respectively. And typical tyrosinase inhibitor, arbutin, inhibited 57.7±2.9% and 65.1±5.0% respectively. Such an similar inhibitory effect of arctigenin and arbutin in B16 melanoma cells may be due to the inhibition of MSH signal pathway rather than the direct inhibition of tyrosinase. Therefore, these results suggest that arctigenin may be useful for the development as whitening agents.