Tetrahydrobiopterin과 Tyrosine Hydroxylase 조절부위의 인위적 변이가 Tyrosine Hydroxylase의 발현에 미치는 영향

조용원,이상도,임정근,이형,김용식,이언,이영재 대한신경과학회 2001 대한신경과학회지 Vol.19 No.5

Significance of Chronic Epilepsy in Glial Tumors

Jung Yong Ahn,Ok-Joon Kim,Kyu Sung Lee,Joong Uhn Choi,Sang Sup Chung,Byung-In Lee 대한신경외과학회 2003 Journal of Korean neurosurgical society Vol.34 No.3

Objective:The aim of this study is to compare the frequency of postoperative epilepsies of patients with chronic as opposed to recent onset epilepsy due to glial tumors in the frontal or temporal lobe with the hypothesis that patients with chronic epilepsy do worse. Methods : We compared the clinical and diagnostic characteristics of the patients(n=73) who had seizures preoperatively to those of the patients(n=153) who did not. Among those who have had seizures preoperatively, we compared those(n=32, chronic seizure group) who had seizures a year or more prior to surgery to those(n=41, acute seizure group) who had seizures within a year prior to surgery. Results : Among the various factors, the frequency of benign pathology and favorable neurological state were higher in seizure group than in non-seizure group(p<0.05). Complex partial seizure and low-grade tumors were frequent in chronic seizure group, whereas simple partial seizure and high-grade tumors were frequent in acute seizure group. Seizure-free rate was significantly higher in acute seizure group than in chronic one(p<0.05). Also, the difference of seizure control rate between surgical strategies were statistically significant(p<0.05). Conclusion : This study indicates that preoperative seizure durations and frequencies have a close relationship with the frequency of postoperative epilepsy of glial tumors. A longer lapse may allow the formation of epileptogenic foci, leading to chronic epilepsy, and eventually having a negative effect on the prognosis of the patients. Factors including histopathological characteristics of the tumor, its location, seizure duration/frequency, and semiology should be taken account of deciding on surgical strategies. Key words:Brain neoplasms;Glioma;Epilepsy.

두개강내 지주막 낭종의 치료 및 결과

안정용,최중언,윤수한,정상섭,이규창 대한신경외과학회 1994 Journal of Korean neurosurgical society Vol.23 No.2

Sixty-four patients with intracranial arachnoid cyst were treated at the Department of Neurosurgery, Yonsei University College of Medicine between 1985 and 1992. Among them, f e rty-eight patients received operation. The purpose of this study was to review our surgical results and find out the most effective surgical procedure for arachnoid cysts in each location. The thirty-one patients with the supratentorial arachnoid cyst showed the mass effect or subdural hematoma on preoperative CT scan. They were treated with marsupialization, cystoperitoneal shunt fenestration with cystoperitoneal shunt, and cystoperitoneal shunt with endoscopy. The fenestration with cystoperitoneal shunt showed the best result in supratentorial arachnoid cysts. The ten patients with the midline arachnoid cyst showed the hydrocephalus on CT scan. They were treated with open fenestration with ventriculoperitoneal shunt, endoscopic fenestration, open fenestration, endoscopic fenestration with ventriculoperitoneal shunt and cystoperitoneal shunt. The endoscopic fenestration showed the best result in midline arachnoid cysts. The seven patients infratentorial arachnoid cysts also presented with hydrocephalus on initial CT scan. The open fenestration with ventriculoperitoneal shunt, open fenestration, endoscopic fenestration, and cystoperitoneal shunt were surgical procedure for these. The cystoperitoneal shunt showed the best result in infratentorial arachnoid cysts. The complications of treatment related with shunt operation, were relieved with shunt revision. Forty-five cases among fortyeight operated cases had good clinical outcome. The most appropriate surgical method for arachnoid cyst were discussed.

Cytotoxicity of Gold Nanorods and Nanowires on Cultivated Neural Precursor Cells.

Kim, Yong-Jung,Yoo, Chan-Jong,Lee, Uhn,Yoo, Young-Mi American Scientific Publishers 2015 Journal of nanoscience and nanotechnology Vol.15 No.8

<P>Given the emergence of nanotherapeutics and nanodiagnostics as key tools in today's medicine, it has become of critical importance to define the interactions of nanomaterials with biological systems. The biomedical applications of nanoparticles (NPs) in chemical sensing, biological imaging, drug delivery, photothermal therapy and cancer treatment have been demonstrated. Gold NPs as new biomedical tools are the focus of research due to their ease of synthesis, chemical stability and unique optical properties. Therefore, there is a need to establish the toxicity, side effects and safety of gold NPs for human applications. To study the in vitro cytotoxicity of gold NPs, we performed MTT assay using two types of gold NPs such as gold nanorods (GNRs) and gold nanowires (GNWs). The percentage cytotoxicity of damaged neural precursor cells (NPCs) that were treated with 100 mg GNRs was 97.5±3.9%; and proportion of damaged NPCs following the administration of the same dose of GNWs was 98.8±0.3%. The cytotoxicity of 10 mg GNRs in NPCs was 54.4±8.3%, whereas it was 98.7±0.6% for the same dose of GNWs. Then, to verify that gold NPs induced apoptotic cell death in NPCs, the LIVE/DEAD Viability/Cytotoxicity assay was performed. We observed that cell death of NPCs increased with an increase in quantity of both types of gold NPs. Cell viability assessed the overall dose-dependent toxicity of NPs in cultured cells. As the results suggest, this study demonstrated that treatment with gold NPs resulted in cellular toxicity in a dose-dependent manner in cultured NPCs.</P>

간호사는 의사와 어떻게 의사소통하는가?

이정우 ( Jung Woo Lee ),박용익 ( Yong Ik Bak ),백승주 ( Seung Joo Baek ),이지언 ( Ji Uhn Lee ),이혜용 ( Hye Yong Lee ),정연옥 ( Yeon Ok Jeoung ) 서울대학교 인문학연구원 2014 人文論叢 Vol.71 No.1

1주파 수신기의 중ㆍ장기선 정확도 분석과 활용성

박운용(Park Woon Yong),이기부(Lee Kee Boo),정공운(Jeong Gong Uhn),홍정수(Hong Jung Soo) 대한토목학회 2003 대한토목학회 학술대회 Vol.2003 No.10

Dose-Dependent Cytotoxicity of Gold Nanoparticles on Human Neural Progenitor Cells and Rat Brain

Yoo, Chan-Jong,Lee, Uhn,Kim, Yong-Jung,Park, Jinny,Yoo, Young-Mi American Scientific Publishers 2019 Journal of Nanoscience and Nanotechnology Vol.19 No.9

Expression of mRNAs for Neurotrophic Factors in Human Neural Stem Cells Derived from Fetal Telencephalon

Young Mi Yoo,Uhn Lee,Yong Jung Kim 대한신경외과학회 2003 Journal of Korean neurosurgical society Vol.33 No.5

Objective:Cellular diversity in the mammalian central nervous system is originated from precursor cells present in the neural ectoderm. The multipotent neural stem cells(NSCs) rapidly proliferate to give rise to transiently dividing progenitors that eventually differentiate into several cell types of neural cells. The authors investigate whether NSCs could differentiate neurons and glia and express neurotrophic factor. Methods:To establish human neural cell lines, we isolated neural stem cells from human fetal telencephalon. Secondly, to investigate the expression of neurotrophic factor, basic fibroblast growth factor(bFGF), brain-derived neurotrophic factor(BDNF) and glial derived neurotrophic factor(GDNF) in rat and human cell, mRNA expressions of bFGF, BDNF and GDNF were detected by the reverse transcripted polymerase chain reaction(RT-PCR) analysis. Results:In the NSCs cultures of embryonic rat striata and human fetal telencephalon, we demonstrated that bFGF induces the proliferation of stem cell, which give rise to spheres of undifferentiated cell that generate neurons and glia. Also, neurotrophic factor transcripts were identified using PCR in rat and human NSCs. Conclusion:These results demonstrate that human NSCs derived from human fetal telencephalon could differentiate neurons and glia and express neurotrophic factors. Therefore, NSCs may be an important key for the therapeutic application of neurotrophic factors. Key words:Neural stem cell;Central nervous system;Telencephalon;bFGF;BDNF;GDNF.

Expression of mRNAs for BDNF and NT-3 in Reactive Astrocytes

Young Mi Yoo,Yong Jung Kim,Uhn Lee 대한신경외과학회 2003 Journal of Korean neurosurgical society Vol.33 No.6

Objective:Astrocytes secrete various neurotrophic factors which act to support the survival and growth of neurons. Reactive astrocytes express an increased level of neurotrophic factors in response to central nervous system injury. We demonstrate that reactive astrocytes could express neurotrophic factors to promote neuronal rescue and generate functional recovery. Methods:To investigate the correlation of neurotrophic factor, brain-derived neurotrophic factor(BDNF) and neurotrophin-3(NT-3) to glutamate-induced reactive gliosis, mRNA expression of BDNF and NT-3 were detected by the RT-PCR technique. Results:Exposure of cultured astrocytes to L-glutamate(1, 100, 200 and 500μM) and scraped astrocytes for 1 day resulted in significant cell damage and we observed mRNA expression of BDNF and NT-3. The maximal expression of BDNF was observed in the control, scraped and L-glutamate treated astrocytes(1μM). The basal expression of BDNF mRNA in astrocytes treated with L-glutamate(100, 200 and 500μM) decreased relative to that of control, scraped and L-glutamate treated astrocytes(1μM). Reactive gliosis, treatment of control astrocytes with glutamate, showed similar pattern for NT-3 mRNA expression. In a word, the basal content of NT-3 mRNA in scrape and L-glutamate(1, 100, 200 and 500μM) expressed similar to that of control astrocytes Conclusion:This study indicates that the reactive astrocytes also expressed mRNA of BDNF and NT-3 as normal astrocytes. Key words:Reactive astrocytes;L-glutamate;Scrape;BDNF;NT-3.

국내 육종업체의 기술 및 규모효율성 분석 -민간육종연구단지 조성을 계기로-

김은순,최세현,조재환,정용관,나정현,Gim, Uhn-Soon,Choi, Se-Hyun,Cho, Jae-Hwan,Jung, Yong-Gwan,Lah, Jung-Hyun 한국유기농업학회 2014 韓國有機農業學會誌 Vol.22 No.1

The purpose of this paper was to measure technical efficiencies and scale efficiencies of Korean seed industry by DEA method, and to identify the factors affecting the efficiencies by Tobit regression model. Survey data of 50 seed companies nationwide were applied for the analysis. The average score of overall technical efficiency for the surveyed companies in 2012 was 0.44, which is decomposed into pure technical efficiency 0.68 and scale efficiency 0.63. A majority of the seed companies exhibited at least one form of inefficiency except a few companies in optimal scale. It was also shown the most companies were operating in the stage of increasing returns to scale, which implies Korean seed companies are mainly in smaller scale than optimal. Additional results suggest that the Center for Private Seed Companies, which will be established at Gimje in 2015, plays an important role to make domestic seed companies improve their scale efficiency as well as pure technical efficiency by way of enlarging their size and co-using the high technology in the Center.