허혈성심질환 환자에 있어 혈액 응고상태의 비교

이미경,송경은,이원길,김재식,박의현,전재은 경북대학교 병원 1997 경북대학교병원의학연구소논문집 Vol.1 No.1

연구배경: 최근에 지혈응고 및 섬유소 용해기능의 손상이 허혈성 심질환의 병인론에 중요한 역할을 한다고 알려져 왔다. 이에 저자들은 허혈성심질환군과 정상대조군에서 PT, aPTT, 섬유소원, α2-antiplasimin, tPA, PAI-I, AT Ⅲ, protein C, XL-FDP를 측정하여, 이들이 허혈성심질환의 발병에 미치는 영향 및 중요성에 연구하고, 혈전용해제 투여전과 투여후의 결과를 비교 분석하여 이들의 검사지표로서의 임상적인 유용성에 관하여 알아보고자 하였다. 방법: 39예의 정상대조군과 급성 심근경색 및 협심증으로 진단된 40예의 환자군에서 platelet-poor-plasma를 얻어 PT, aPTT, 섬유소원은 ELECTRA 1000C(Medical laboratory automation, Inc., USA)를 이용하여 측정하였고, α2-antiplasmin는 색소법으로 SPECT-ROLYSER α2-antiplasmin(Biopool AB, Canada)kit를, tPA와 PAI-1은 면역효소법으로, Protein C는 응고법으로 Bioclot?? Protein C(Biopool AB, Canada) kit를 사용하여 측정하였다. XL-FDP는 라텍스응집법으로 DIMERTESTR Ⅱ Latex Kit(AGEN Biomedical Limited, Australia)를 이용하여 측정하였다. 결과: 환자군과 정상대조군의 비교에서 에서 PT, aPTT, 섬유소원, PAI-1 및 ATⅢ는 두 군간에 통계학적으로 유의한 차의를 나타내었고 환자군에서는 62.5%에 해당하는 25예에서 증가된 소견을 보여 주었다. 환자군에서 혈전용해제 투여전과 투여후의 결과비교에서 PT와 섬유소원에서 유의한 차이가 있었으며, α2-antiplasmin, PAI-1 및 protein C는 유의하지는 않았지만 감소하는 경향을 나타내었다. 혈전용해제 투여후 1 시간결과를 대조군과 비교해 볼 때 PT와 tPA는 유의하게 증가하였고 섬유소원과 ATⅢ는 의의있게 감소하였다. 7일째 결과는 모든 지수에서 정상대조군과 유의한 차이가 없었다. 결론: PT, 섬유소원의 검사가 허혈성 심질환의 진단과 경과관찰에 도움을 줄 수 있으며, tPA, PAI-1, ATⅢ등과 같이 관찰함으로서 진단뿐만 아니라 예후와 재발위험성에 대한 예측 등 임상적으로 중요한 검사지표로서의 가능성을 나타냄을 알 수 있었다. Background: Many studies have shown that thrombus formation has important role in the pathogenesis of ischemic heart disease(IHD) and impaired fibrinolysis is associated with an increased risk of myocardial infacrtion. Forty patients with IHD and 39 normal controls were studied about blood coagulation, fibrinolytic, inhibitory proteins to evaluate their usefulness in diagnosing, predicting prognosis and monitoring after thrombolytic therapy in IHD. Method: Platelet-poor-plasma were obtained from the 3.8% trisodium citrate treated blood and prothrombin time(PT), activated partial thromboplastin time(aPTT), firbrinogen, α2-antiplasmin, tPA,PAI-1, antithrombin Ⅲ(ATⅢ), protein C. crosslinked fibrin degradation products(XL-FDP) were measured by appropriate functional or immunological assays. Results: The IHD group showed significant increases in PT,aPTT, fibrinogen, PAI-I and XL-FDP and significant reduction in AT Ⅲ concentration. No significant changes were observed between myocardial infarction and angina pectoris subgroups in the IHD. After thrombolytic therapy, PT and fibrinogen showed significant changes. In comparsion with normal controls, post 1 hour results showed significant changes in PT, fibrinogen, tPA, ATⅢ but no significant changes in post 7 days results. Conclusion: Our findings suggest that measurements of PT and fibrinogen are helpful for diagnosis in patients with IHD. And combined with tPA, PAI-1 and AT Ⅲ, they may be useful test for monitoring after thrombolytic therapy, predicting prognosis and risk of recurrence in IHD patients.

SO₂와 NOx 동시제거를 위한 흡수제의 반응특성

이형근,최원길,박영성 大田大學校 環境問題硏究所 1997 환경문제연구소 논문집 Vol.2 No.-

Recently the more stringent emission standards of government promulgate the incentive for developing the technology capable of simultaneous removal of SO₂ and NOx from flue gas. This study was to develope an enhanced absorbent in reactivity and adsorption ability for removing SO₂and NOx from flue gas. The characteristics of spray drying absorption(SDA) process and status of SDA process were studied, as well as analysis of properties of absorbent and its manufacturing method. CaO, MgO, and NaHCO₃were selected as a alkali reagent, and they were mixed with fly ash(FA) or activated carbon powder(AC) and their the reactivity and adsorption ability toward SO₂and NOx were greatly enhanced through got water curing process. Among the highest. The surface area of MgO mixed with FA(30%) was greater than NaHCO₃, but the SO₂and NOx removal performance of MgO mixed with FA(30%) was much lower than that of NaHCO₃. The removal quantity of SO₂and NOx per mole of NaHCO₃mixed with FA(30%) was 2.7 mole, this meant that the effect of physical adsorption was greater than chemical reaction between absorbent and acid gases.

새로운 Transfusion-transmitted Virus (TTV)의 성상

이원길 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

호흡기 완자의 대조군의 혈청학적검사에 의한 Chlamydia Pneumoniae의 감염률

이해숙,천병력,진수희,이원길 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

HBsAg양성 원발성간암에 있어서 HBV-DNA표지자의 PCR성적

이원길,김재식,김정철,서장수,강은자,이중원,김기연,송경은,양정선 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

목적 : HBV-DNA를 정성적 그리고 정량적으로 검출함으로써 강한 전염성과 높은 유병율로 인해 사회적으로 문제가 되는 B형 간염과 원발성간암의 연관성을 알아보고자 하였다. 대상 및 방법 : B형 간염 표면항원(HBsAg)이 양성을 보이는 원발성간암 환자 38명을 대상으로 하였으며 HBV-DNA 중합효소연쇄반응법을 이용하여 정성적 검출 그리고 luminometry법을 이용하여 정량적 검출을 하였다. 결과 : HBV-DNA 중합효소연쇄반응법과 luminometry법의 양성율은 각각 57.9%, 39.5%를 보였다. 그리고 HBsAg과 HBeAg가 동시에 양성을 보인 11명에서는 HBV-DNA 양성율이 81.8%를 보였다. 결론: HBV-DNA 중합효소연쇄반응법의 양성율이 luminometry법에 의한 양성율보다 높은 결과를 보였고 HBV-DNA 검사는 B형 간염을 조기 발견하고 치료하여 만성간질환으로의 진행을 예방하고 특히 원발성간암의 병율을 낮추는데 많이 기여할 것으로 사료되었다. Background : Primary hepatocellular carcinoma(PHCC) is the most common malignant neoplasm in most countries of Asia and Africa. Hepatitis B virus is known to be strongly related to the pathogenesis of PHCC. HBV-DNA PCR and HBV-DNA quantitation assay were attempted to apply to 38 cases of HBsAg positive PHCC. Methods : The ordinary HBV markers(HBsAg, anti-HBs, anti-HBc(Ig-G and -M), HBeAg and anti-HBe) were examined with ELISA. Qualitative screening of HBV-DNA PCR and HBV-DNA quantitation-luminometric measurement were performed. Results : Among 38 cases of HBsAg positive PHCC, positive rates for HBV DNA-PCR and HBV DNA-quantitation were 57.9% and 39.5%, respectively. And 11 cases which were positive for both HBsAg and HBeAg showed 81.8% positive rate in HBV-DNA PCR. Conclusion : For 38 cases of HBsAg positive PHCC, the positive rate for HBV DNA-PCR was higher than HBV-DNA quantitation-luminometry. In HBV-DNA PCR, both HBsAg and HBeAg positive cases showed high positive rate.

제한속도 규제와 그 영향에 대한 분석

이원영,성낙문,박길수 한국안전교육학회 2002 한국안전교육학회지 Vol.5 No.2

This study purposes to provide a rational direction to the policies associated with speed limits of Korea through literature reviews. As a result of analysis, it is generally accepted that the higher running speed and speed limit bring about the more traffic accident frequencies and the higher severity. Traffic safety experts strongly confirm that speed variances affect the occurrences of the traffic crashes. An inappropriate speed limit increases the speed variances and becomes a factor of the occurrences of traffic crashes. Accordingly, the speed limit should be determined in appropriate manner. This means that speed limit should be installed considering running speeds of vehicles and geometries of the road. There have not been reliable researches related to speeds and traffic crashes in Korea, even though the speeds have an very important role for the occurrence of traffic crashes. Therfore it is required that researches related to the traffic crash and traffic safety should be conducted actively in Korea.

일본인 한국어학습자의 한국어문법교육·(1)

이길원 동아대학교 어학연구소 2002 언어와 언어교육 Vol.17 No.-

B형간염 바이러스(Hepatitis B Virus:HBV)의 성상과 HBV DNA 정량검사

이원길 대한임상미샐물학회 2003 Annals of clinical microbiology Vol.6 No.2

1-Bromoacetylpyrene 誘導體化劑를 利用한 Carboxyl基 含有成分의 分析에 관한 硏究(Ⅰ) : 高級不飽和脂肪酸의 HPLC에 依한 定量 Determination of Unsaturated Fatty Acids by HPLC

李允中,曺正吉,朴元敎,李康春 成均館大學校 科學技術硏究所 1988 論文集 Vol.39 No.1

A simple and highly sensitive method for the determination of unsaturated fatty acids (C_16:1, C_20:4, C_20:5) is described. I-Bromoacetylpyrene was used as the pre-column fluorescent labelling reagent for HPLC. Fatty acids were derivatized quantitatively into fluorescent compounds by treating with 1-bromoacetylpyrene in the presence of 18-crown- 6 in acetonitrile. The optimum conditions for the derivatization such as, concentrations of KOH, 18-crown-6, and 1-bromoacetylpyrene, reaction temperature and reaction time, were investigated. The derivatives were separated on a reversed phase column (LiChrosorb RP-8, 5 ㎛) using the tertiary mixture of acetonitrile, methanol, and water as the mobile phase. The effluent was monitored by fluorometer (excitation wavelength; 366 nm, emission wavelength; 454 nm). Linearities of calibration curve were obtained between 5.0 p mol and 40.0 p mol. The detection limit of fatty acids was 1 p mol in a 20μl of injection volume.

공동주택의 포름알데히드 농도특성 분석에 관한 연구

이윤규,한길원 대한건축학회 2003 대한건축학회논문집 Vol.19 No.10

This study aims to investigate the concentration of formaldehyde, which is one of the most well-known VOCs(Volatile Organic Compounds), and analyze the characteristic of its concentration in newly-constructed apartment houses. The field measurement was conducted in different five apartment house complexes located in Seoul and Kyonggi province. Indoor concentration of formaldehyde showed between 0.018ppm and 0.607ppm. The average concentration was 0.150ppm and above the foreign standards. It result from the new construction. Also, the higher measurement position and the bigger measurement extent, the greater concentration of formaldehyde. And it was treated by the statistic program to examine the objective relations among the concentration of formaldehyd, measurement factors and basic thermo-environment elements. As a result, the temperature, the relative humidity and the measurement position had a validly effect on the concentration of formaldehyde and the temperature extremely influenced it.