High-speed Three-dimensional Surface Profile Measurement with the HiLo Optical Imaging Technique

Kang, Sewon,Ryu, Inkeon,Kim, Daekeun,Kauh, Sang Ken Optical Society of Korea 2018 Current Optics and Photonics Vol.2 No.6

Various techniques to measure the three-dimensional (3D) surface profile of a 3D micro- or nanostructure have been proposed. However, it is difficult to apply such techniques directly to industrial uses because most of them are relatively slow, unreliable, and expensive. The HiLo optical imaging technique, which was recently introduced in the field of fluorescence imaging, is a promising wide-field imaging technique capable of high-speed imaging with a simple optical configuration. It has not been used in measuring a 3D surface profile although confocal microscopy originally developed for fluorescence imaging has been adapted to the field of 3D optical measurement for a long time. In this paper, to the best of our knowledge, the HiLo optical imaging technique for measuring a 3D surface profile is proposed for the first time. Its optical configuration and algorithm for a precisely detecting surface position are designed, optimized, and implemented. Optical performance for several 3D microscale structures is evaluated, and it is confirmed that the capability of measuring a 3D surface profile with HiLo optical imaging technique is comparable to that with confocal microscopy.

Rosacea: our inquiry from epidemiology to microbiome

( Sewon Kang ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.1

Behçet’s disease risk association fine-mapped on the <i>IL23R</i> – <i>IL12RB2</i> intergenic region in Koreans

Kang, Eun Ha,Kim, Sewon,Park, Min Young,Choi, Ji Yong,Choi, In Ah,Kim, Min Jung,Ha, You-Jung,Lee, Eun Young,Lee, Yun Jong,Lee, Eun Bong,Kang, Changwon,Song, Yeong Wook BioMed Central 2017 ARTHRITIS RESEARCH AND THERAPY Vol.19 No.-

<P><B>Background</B></P><P>Behçet’s disease (BD) susceptibility had been associated with single-nucleotide polymorphisms (SNPs) in <I>IL23R</I>–<I>IL12RB2</I>, <I>IL10, STAT4,</I> or <I>ERAP1</I> locus in Japanese, Turkish, Chinese, and other populations, but not in a Korean genome-wide association study (GWAS). We aimed to fine-map BD risk association of these four loci using extensive imputation and additional genotyping for replication.</P><P><B>Methods</B></P><P>In the discovery phase, 369 patients with BD enrolled in the previous Korean GWAS and 2000 controls retrieved from a population-based cohort of healthy Koreans were imputed for their genotypes of all SNPs in the four loci using the Asian data of the 1000 Genomes Project as reference. For genotype imputation of <I>ERAP1</I> SNPs, the adjacent <I>ERAP2</I> SNPs were also covered. For the 10 most significantly associated SNPs (8 imputed and 2 GWAS-genotyped), an additional 84 patients with BD and 283 healthy controls were genotyped for replication. The results from the discovery and replication phases were pooled for meta-analysis using the Mantel-Haenszel test to estimate the odds ratio (OR) and 95% confidence interval (CI).</P><P><B>Results</B></P><P>An <I>IL23R</I>–<I>IL12RB2</I> intergenic SNP rs1495965 was significantly associated with BD risk (OR (95% CI) = 1.5 (1.3, 1.7), <I>P</I> = 2.5 × 10<SUP>−7</SUP>) in the pooled meta-analysis of the discovery (1.4 (1.2, 1.7), <I>P</I> = 4.9 × 10<SUP>−7</SUP>) and replication (1.9 (1.3, 2.6), <I>P</I> = 6.0 × 10<SUP>−4</SUP>) phases. BD risk association was fine-mapped on the intergenic region rather than the two flanking genes, as rs1495966 and rs4655535, almost perfectly correlated with rs1495965 (<I>r</I><SUP>2</SUP> = 0.99), were also located in the same intergenic region. Consistent with previous reports, the <I>P</I> values tended to be lower within <I>IL23R</I> than <I>IL12RB2</I>. On the other hand, several <I>IL10</I> SNPs were suggested for association in the discovery phase but all failed in the replication phase. No SNP in <I>ERAP1</I>–<I>ERAP2</I> and <I>STAT4</I> was suggested even in the discovery phase.</P><P><B>Conclusions</B></P><P>BD susceptibility association was fine-mapped on the intergenic region between <I>IL23R</I> and <I>IL12RB2</I> as marked by three correlated SNPs, rs1495965, rs1495966, and rs4655535.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s13075-017-1435-5) contains supplementary material, which is available to authorized users.</P>

Comparison of Embryo Culture Efficiency in Early Maturing Peach (Prunus persica) according to Developmental Stages of Embryo and Medium Composition

Sewon Oh,Byeonghyeon Yun,Se Hee Kim,Sang-Yun Cho,Namhee Jung,Kyung Ran Do,Kang Hee Cho,Hyun Ran Kim 한국원예학회 2023 한국원예학회 학술발표요지 Vol.2023 No.10

Convergence ability and convergence education needs in nursing

강세원, 전은미 배재대학교 자연과학연구소 2019 自然科學論文集 Vol.30 No.1

The purpose of this study was to investigate the convergence ability, convergence education needs in nursing students. The participants of this study were 131, first and third grade nursing students. Data collection was conducted from August to September 2019 and structured questionnaires were used. Collected data were analyzed using the IBM SPSS Statistics 22.0 Program. Data analysis was performed using descriptive analysis, independent samples t-test. As a result, the convergence ability score was 3.50 (out of 5) and the convergence education need was 2.33 (out of 5). The convergence ability had a difference according to grade level, and first grade was higher than third grade (t=3.596, p<0.001). Also, the group with higher convergence education need had higher convergence ability score (t=-3.098, p <0.002). In order to play a responsible role in the future, it is necessary to develop nursing students' convergence capacity.

Preliminary Experiment of Radioactive Caesium Analysis in Seawater Using Ammonium Phosphomolybdate Coprecipitation

Sewon Park,Hyojin Kim,Jieun Lee,Yeong-rok Kang 한국방사성폐기물학회 2023 한국방사성폐기물학회 학술논문요약집 Vol.21 No.1

Cs-137, a radioactive isotope of caesium, is a commonly occurring fission product that is generated during the nuclear fission of U-235 and other fissionable isotopes in both nuclear reactors and weapons. Due to its long half-life of about 30 years and propensity to accumulate in sediments and marine organisms, Cs-137 is considered a major radionuclide for environmental radioactivity monitoring. In April 2021, as the Japanese government decided to discharge Fukushima contaminated water into the sea, the monitoring of marine radioactivity in South Korea has become increasingly significant. In this study, as an initial step towards establishing a standardized procedure for analyzing radioactive caesium in seawater, the radioactivity of Cs-137 was analyzed on a 2 L of seawater spiked with 10 Bq of Cs-137 standard solution supplied by KRISS. The seawater was collected from Im-nang Beach, situated at a distance of approximately 2 kilometers from DIRAMS. The radioactivity of Cs-137 in seawater was determined according to the improved AMP procedure presented by M.Aoyama in 2000. The seawater was pretreated using Ammonium Phosphomolybdate (AMP) coprecipitation, which has a high selectivity for caesium (Kd = ~5500), and the activity of Cs-137 was determined by gammaspectroscopy and subsequently corrected via the weight yield. The weight yield of the dried AMP/Cs compound was more than 93%. For the gamma-spectroscopy analysis, the AMP/Cs compound was dissolved in a cylindrical U8 beaker with NaOH to ensure that its shape and volume were consistent with the CRM (KRISS, 221U890-1) used to calibrate the detector. The dissolved compound was then positioned directly onto the detector housing and subjected to a measurement duration of 80,000 seconds utilizing a p-type HPGe (Ortec, GEM60) with a relative efficiency of 54%. The activity of Cs-137 was determined to be 10.81 Bq, confirming the reproducibility of the AMP coprecipitation and weight yield methods. The present experiment was carried out using a 2 L sample, but a large volume of seawater would be required to achieve a sufficient minimum detectable activity (MDA) for Cs-137 in natural seawater. Thus, a standardized procedure for analysis of radioactive caesium in natural seawater will be established through the analysis of a large volume of seawater in future studies.

Four cases of median nail dystrophy treated with intralesional steroid injection

( Sewon Hwang ),( Hwa Young Jung ),( Jin Hee Kang ),( Miri Kim ),( Hyun Jeong Park ),( Baik Kee Cho ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

Median nail dystrophy(MND) is an uncommon and idiopathic dystrophic change, typically showing a central nail groove, beginning at or distal to the proximal nail fold, from which small lateral fissures may emerge. The onset of this nail dystrophy has occasionally been associated with either prior local trauma or initiation of some medical treatment. Various agents have been tried to treat the disease, but it is often resistant to many topical treatments. We performed a clinical study of four patients who were diagnosed with MND, between 2008 and 2014 at the Dermatology Department of Yeouido St. Mary’s Hospital. In three cases, thumbnails were affected and in one case, one 3rd fingernail was affected. All patients were male, with a mean age of 54.3 years (range, 33-63 years). One patient had repeated habitual nail biting. The involved nails showed typical clinical features of MND,including longitudinal groove or split involving one or both thumbnails. All the patients were treated by intralesional triamcinolone injection once a month. After treatment, the patients showed good responses. Here, we report four cases of MCND which were almost cleared by intralesional triamcinolone injection. We suggest intralesional triamcinolone injection as a safe and effective option for the treatment of MND.

R-1336mzz(Z) 냉매를 적용한 유하액막식 증발 열전달 특성에 관한 실험적 연구

이세원(Sewon Lee),변선웅(Seonwoong Byun),백창현(Changhyun Baek),강훈(Hoon Kang),김용찬(Yongchan Kim) 한국유체기계학회 2023 유체기계 연구개발 발표회 논문집 Vol.2023 No.7

Expression-associated polymorphisms of <i>CAV1</i> - <i>CAV2</i> affect intraocular pressure and high-tension glaucoma risk

Kim, Sewon,Kim, Kyunglan,Heo, Dong Won,Kim, Jong-Sung,Park, Chan Kee,Kim, Chang-sik,Kang, Changwon Molecular Vision 2015 Molecular vision Vol.21 No.-

<P><B>Purpose</B></P><P>The human <I>CAV1</I>-<I>CAV2</I> locus has been associated with susceptibility to primary open-angle glaucoma in four studies of Caucasian, Chinese, and Pakistani populations, although not in several other studies of non-Korean populations. In this study with Korean participants, the <I>CAV1</I>-<I>CAV2</I> locus was investigated for associations with susceptibility to primary open-angle glaucoma accompanied by elevated intraocular pressure (IOP), namely, high-tension glaucoma (HTG), as well as with IOP elevation, which is a strong risk factor for glaucoma.</P><P><B>Methods</B></P><P>Two single nucleotide polymorphisms (SNPs) were genotyped in 1,161 Korean participants including 229 patients with HTG and 932 healthy controls and statistically examined for association with HTG susceptibility and IOP. One SNP was rs4236601 G>A, which had been reported in the original study, and the other SNP was rs17588172 T>G, which was perfectly correlated (<I>r</I><SUP>2</SUP>=1) with another reported SNP rs1052990. Expression quantitative trait loci (eQTL) analysis was performed using GENe Expression VARiation (Genevar) data.</P><P><B>Results</B></P><P>Both SNPs were associated with HTG susceptibility, but the rs4236601 association disappeared when adjusted for the rs17588172 genotype and not vice versa. The minor allele G of rs17588172 was associated significantly with 1.5-fold increased susceptibility to HTG (p=0.0069) and marginally with IOP elevation (p=0.043) versus the major allele T. This minor allele was also associated with decreased <I>CAV1</I> and <I>CAV2</I> mRNA in skin and adipose according to the Genevar eQTL analysis.</P><P><B>Conclusions</B></P><P>The minor allele G of rs17588172 in the <I>CAV1</I>-<I>CAV2</I> locus is associated with decreased expression of <I>CAV1</I> and <I>CAV2</I> in some tissues, marginally with IOP elevation, and consequently with increased susceptibility to HTG.</P>

생산성 영향요인을 고려한 건설현장 토공사 시뮬레이션 모델 개발

사세원 ( Sa Sewon ),이찬우 ( Lee Chanwoo ),조훈희 ( Cho Hunhee ),강경인 ( Kang Kyeong-in ) 한국건축시공학회 2021 한국건축시공학회 학술발표대회 논문집 Vol.21 No.1

Earthwork is the starting work of construction process, which has the greatest impact on the productivity of the construction project among the works. However, it is difficult to derive accurate productivity becuase the earthwork plan is affected by geological condition of the jobsite. Therefore, a simulation model for productivity analysis of earthwork was developed using CYCLONE modeling method in this study. In this paper, simulation model was made considering the impact factors of earthwork productivity. The proposed model can be utilized for sensitivity analysis in future studies.