Time-evolving genetic networks reveal a NAC troika that negatively regulates leaf senescence in <i>Arabidopsis</i>

Kim, Hyo Jung,Park, Ji-Hwan,Kim, Jingil,Kim, Jung Ju,Hong, Sunghyun,Kim, Jeongsik,Kim, Jin Hee,Woo, Hye Ryun,Hyeon, Changbong,Lim, Pyung Ok,Nam, Hong Gil,Hwang, Daehee National Academy of Sciences 2018 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.115 No.21

<▼1><P><B>Significance</B></P><P>Leaf senescence is regulated in a complex manner, involving time-dependent interactions with developmental and environmental signals. Genetic screens have identified key regulators of senescence, particularly late-stage senescence regulators. Recently, time-course gene-expression and network analyses, mostly analyses of static networks, have predicted many senescence regulators. However, senescence is defined by time-evolving networks, involving the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks of NAM/ATAF/CUC (NAC) transcription factors, central regulators of leaf senescence in <I>Arabidopsis</I>, via time-course gene-expression analysis of NACs in their mutants. These time-evolving networks revealed a unique regulatory module of NACs that controls the timely induction of senescence-promoting processes at a presenescent stage of leaf aging.</P></▼1><▼2><P>Senescence is controlled by time-evolving networks that describe the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks for NAM/ATAF/CUC (NAC) transcription factors in <I>Arabidopsis</I> during leaf aging. The most evident characteristic of these time-dependent networks was a shift from positive to negative regulation among NACs at a presenescent stage. ANAC017, ANAC082, and ANAC090, referred to as a “NAC troika,” govern the positive-to-negative regulatory shift. Knockout of the NAC troika accelerated senescence and the induction of other <I>NAC</I>s, whereas overexpression of the NAC troika had the opposite effects. Transcriptome and molecular analyses revealed shared suppression of senescence-promoting processes by the NAC troika, including salicylic acid (SA) and reactive oxygen species (ROS) responses, but with predominant regulation of SA and ROS responses by ANAC090 and ANAC017, respectively. Our time-evolving networks provide a unique regulatory module of presenescent repressors that direct the timely induction of senescence-promoting processes at the presenescent stage of leaf aging.</P></▼2>

3차원 자기 트랜지스터의 제작 및 특성

정우철,백영희,남태철 嶺南大學校 工業技術硏究所 1993 工業技術硏究所論文集 Vol.21 No.1

The conventional magnetic sensors are generally designed for measuring one component of magnetic field vector perpendicular or parallel to the device surface. We need a magnetic sensor which can detect three components of a magnetic field vector in order to measure the magnetic field precisely, because the magnetic field is a vector which has three components. We have fabricated magnetotransistor using standard CMOS technology for measuring all three components of a magnetic field vector at a time. The magnetotransistor is npn type and is consisted of a common emitter, four base, and four collector pairs on each side of emitter for sensing magnetic field. The collector pairs on each side of emitter is used ofr detection z-component of magnetic field which is applied to the device surface perpendicularly. And each collector pairs which is sat opposite symmetrically with emitter in the center is used for detection x-, y- components of magnetic field which is applied to the device surface in parallel. We have measured the characteristics of the voltage difference of the collector pairs and sensitiviti according to the variation of magnetic field. The results have presented good linearities to the magnetic field. Under the uniform magnetic field, we have also measured the variation of sensitivity according to the variation of substrate and base current.

진화의 관점에서 본 ADHD의 기원

손정우, 남정현 충북대학교 의과대학 충북대학교 의학연구소 2016 忠北醫大學術誌 Vol.26 No.1

본문참고

TMAH에 의한 이방성 식각을 이용한 3차원 자기센서의 제작

정우철,남태철 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

This paper present an anisotropic etching in TMAH technique used in the fabrication of three-dimensional magnetic field vector sensor based on angled Hall plate structure. This sensor design relies on simultaneously detecting all magnetic field vector components using Hall plates that are imbedded into the silicon [111] sloped-surface of bulk micromachined cavity by the anisotropic etching of [100] silicon. The fabricated Hall elements has relatively improved sensitivity compare to convensional Hall elements for three-dimensional magnetic field sensing. The product sensitivity of 547V/AT at the supply current of 1.0mA has been achieved.

소집단 역할놀이와 토의를 통한 고등학생들의 천문개념 이해

정남식,우종옥,정진우 한국교원대학교 과학교육연구소 1996 청람과학교육연구논총 Vol.6 No.1

The purpose of this study was to apply the instructional strategies for conceptual change prescribed by Posner et al(1982) to the astronomic content domain taught in the elementary and middle school and to analyze the characteristics of students' knowledge revealed in the test before, during and after the instruction. Also, it was to investigate the intercorrelation of cognitive levels, spatial ability and science achievement. The major findings of this study are as follows: 1. Students had a great variety of misconceptions related to the motion of the moon before the instruction, that is, the phases, the names of phases and the cause of changing phases by the moon's orbit about the earth, the moon's appearance and location at the given time, the relative positions of earth, moon and sun during a lunar eclipse, the cause that a full moon is not at the line of node once a month. In the analysis of students' responses concerning the cause of changing phases of the moon and a lunar eclipse, the results indicate that the great majority of students had rote learning rather than meaningful learning in the middle school. 2. Students' reponses during the instruction concerning the changing phases of the moon and the predictive knowledge about the motions of the earth and the moon were analyzed. 1) According to the results of the test given before and after experiment, after discussion, achievement score of the whole of subjects and groups in both preformal and formal cognitive levels appeared to increase linearly. 2) There was no statistically significant differences of achievement scores before and after experiment, after discussion between preformal group and formal group in cognitive levels. 3. Distribution of achievement scores according to the whole of subjects and groups in preformal and formal cognitive levels shows that there was a statistically significant difference between pretest and posttest. 4. Types of conceptual changes concerning the cause of changing phases of the moon that occurred from pretest to posttest were classified as accommodation, incomplete accommodation, assimilation, no change and no model. Six of the seven students starting instruction with alternative frameworks didn't sustain those alternative models throughout instruction. Five of these six students accommodated completely and the last one partially. Seventy-nine percentage of students taking instruction with fragmental models assimilated correct propositions at the end of the instruction. These results suggest that conceptual change model prescribed by Posner et al(1982) has promised the meaningful learning to students taking with fragmental models, especially in cases where students with misconception enter instruction. 5. High correlation between achievement score of simple-recall items and that of written items in pretest and posttest indicates that the higher students got the score in simple-recall items the better they also performed in written items. However, there was no statistically significant differences among cognitive levels, spatial ability and science achievement in the whole of subjects and groups according to the cognitive levels.

Transcriptome profiling of olive flounder responses under acute and chronic heat stress

Woo‑Jin Kim,이규빈,Dain Lee,Hyun‑Chul Kim,Bo‑Hye Nam,Hyungtaek Jung,이선주,Kyunghwan Kim 한국유전학회 2021 Genes & Genomics Vol.43 No.2

Background The olive founder (Paralichthys olivaceus) is a saltwater fsh, which is valuable to the economy. The olive founder strives to adapt to environmental stressors through physiological, biochemical, and transcriptional responses. The rise in water temperature threatens the growth, development, reproduction, and survival of olive founder. Each organ in the olive founder can diferentially respond to heat stress. Objectives The purpose of this study is to investigate organ-specifc transcriptional changes in olive founder tissues during heat stress. Methods In this study, transcriptome dynamics of the gill, liver, and muscle of olive founder to acute or chronic heat stress were investigated. Results Principal component analysis plotting revealed that the transcriptome of each organ is quite separated. K-means clustering, gene ontology, and Kyoto Encyclopedia of Genes and Genomes pathway analysis showed the diferential transcriptome responses of each organ to heat stress. Heat stress commonly afects the pathways involved in the correct protein folding, DNA repair, and cell cycle. Conclusion Our results may provide a valuable molecular basis of heat acclimation in fshes.

한국과 일본의 건강증진정책 비교

남은우,조은주,남정자,Toshihiko HASEGAWA,Yoshi FUJISAWA 고신대학교보건과학연구소 2003 보건과학연구소보 Vol.13 No.-

활성탄 제조용 하수슬러지 활성탄화로 개발

정동현,이우주,전영남 조선대학교 환경공해연구소 2007 環境公害硏究 Vol.20 No.1

Sludge treatment has been studied with many researcher since disposal problem of sewage sludge was became powerfully. But there was still the problem of secondary pollution and heavy metal leaching in the sewage sludge treatment process. To solve the problem, the treatment was to produce active carbon through the carbonization and the steam activation. In this study attempts to optimum design a activated carbonizer and optimum operation conditions and the technology as changing the variables. parametric screening studies were carried out for variations of the following variables : input of steam "10∼80 g/min" and carbonized time "12∼31.2 min". At the result of this is chosen, that input of steam "35 g/min" and carbonized time "31.2 min". At this time, Iodine adsorptivity and output particle diameter shown that 245.5 ㎎/g and under 4 mm. And also, by using photograph and SEM were confirmed the pore development.

공구강상의 TiN 및 TiCN 피막의 기계적 성질에 관한 연구

鄭祐昌,白英男 慶熙大學校 材料科學技術硏究所 1996 材料科學技術硏究論集 Vol.9 No.-

TiCN and TiN films were deposited on high speed steel by cathode arc ion plating process. The experiment variables were the flow rates of reactive gas (CH_(4):N_(2)) under fixing deposition pressure, arc current, bias voltage and jig rpm and distance between target and substrate. The structure and composition of the TiN and TiCN films were analyzed by XRD, SEM and AES. Mechanical properties of TiN and TiCN films were compared through micro hardness test, adhesion test. Main results through this study are summarized as followings.

골격성 Ⅲ급 부정교합자의 전치부 수직피개도에 영향을 미치는 요소 - 개교군과 피개군간의 비교

정정화,남형진,강상욱,손우성 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25