두경부 편평상피세포암 세포주의 VEGF 아형의 분포와 종양증식 및 이종이식성 : relation to xenotransplantability and tumor progression in mice.

김동욱,이종대,박진규,이재형,이병돈,장혁순 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.2

Overexpression of vascular endothelial growth factor(VEGF) is related to tumor progression and xenotransplantability in various human solid tumors, but the specific impact of the VEGF-subtypes is still under discussion. The aim of this study was to analyse a possible association of the major VEGF-isoforms and the growth characteristics of xenotransplanted human head and neck squamous cell carcinoma tumors in nude mice. Seven squamous cell carcinoma cell lines were analysed by quantitative RT-PCR using the Taqman^(TM)-System. We investigated the expression of VEGF-total-mRNA and of the major subtypes VEGF-121, -165, and -189 by using subtype specific primers. The cell lines were xenotransplanted in three mice each, and the data of tumor growth and progression were correlated to the expression of VEGF-isoforms. We also investigated an "growth response rate" measured by tumor growth per detected VEGF-level. Six out of the seven cell lines analysed expressed all isoforms of VEGF in different quantities. One cell line expressed generally low levels of VEGF and no VEGF-189 at all, In this cell line xenotransplantation failed in one mice out of three. In a second cell line transplantation failed in one out of seven mice, too. Success rates for the other five cell lines were 100%. The cell lines with higher transplantation success were expressing higher VEGF-121/165-189 ratios comparing to those without success. In contrast, linearity of tumor growth and lack of necrosis were associated with a lower VEGF-121/165-189 ratio. The findings demonstrate a predominant expression of VEGF-165 and VEGF-189, compared VEGF-121. In highly proliferating tumors this rate appeared to be about 10 times higher than in low proliferating tumors. We conclude that the ratio between the VEGF-subtypes during tumor implantation and growth is a prerequisite for progression and hypothesize an individual and different response of each tumor cell line to VEGF.

운동 유발성 횡문근 융해를 동반한 요로결석 2례

허진,최원혁,조진혁,함영희,홍정범,정성규,김현,허동 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Two men were admitted to hospital with flank pain, hematuria, which was diagnosised as ureteral stone. Elevation of aspartate aminotransferase (AST) without typical pattern of toxic hepatitis was observed. Careful history taking, several laboratory tests, abdominal and pelvis computered tomography was done. Findings from theses examinations supported the clinical diagnosis of ureteral stone complicated of exercised induced rabdomyolysis. Early recognization of rhabdomyolysis in clinical setting is important, because clinical manifestations have ranged from asymtomatic elevation of creatine kinase to acute renal failure which is a life threating medical emergency. Authors report two cases of exercised induced rhabdomyolysis initially admittied as ureteral stone managed with hydration

운동이 비만 여성의 심근산소소비량과 심전도 QTc 간격에 미치는 영향

이혁종,박윤정,김용권,한구석,진영수 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of exercise staining on MVO_2 and electrocardiogram QTc interval in obese woman and to propose the desirable method for weight loss. Subjects were fifteen obese women for this study. 8 subjects were prolonged QTc interval group and 7 subjects were normal QTc interval. They participated in exercise program consisted of aerobic exercise and weight training exercise at A Medical Center. The body composition including Boyd Weight, BMI, WHR and % Body Fat was measured. The resting and exercise(Bruce protocol 7th min) rate-pressure product(RPP) could be measured with HR and systolic BP for MVO_2 measurement. The electrocardiogram QTc interval was calculated with Bazzett's formula using automated software program. The measured data of this study were as follows: 1) After participating the exercise program consisted of aerobic exercise and weight training exercise, Body Weight, BMI, WHR and % Body Fat were significantly changed in two groups. (p<.05). 2. After participating in the exercise program consisted of aerobic exercise and weight training exercise, the resting and exercise MVO_2 using RPP were significantly changed in two groups(p<.05). 3) After participating in the exercise program consisted of aerobic exercise and weight training exercise, the electrocardiogram QTc interval were decreased but were not significantly changed in prolonged QTc interval group. (p<.29). However, it was significantly decreased in prolonged QTc interval(p<.00).

UGR(Unified Glare Rating)의 보정 및 불쾌글레어 주관평가를 위한 실험적 연구

이진숙,김병수,권혁일 대한건축학회 2003 대한건축학회논문집 Vol.19 No.7

Glare in one of the major factors influencing comfort appraisal for lighting. Although a predictive formula for such glare has been proposed by CIE, it has not been investigated for domestic adjustment. The correlation between glare and the closely related dazzling-sensation has not been investigated enough. In the study, subjective discomfort glare appraisal and dazzling-sensation appraisal for discomfort glare was performed to produce a corrected UGR formula. Analyses between appraisal for dazzling-sensation and results of subjective appraisal for discomfort glare were performed to produce a formula that could represent the correlation between discomfort glare and dazzling-sensation. The results of the comparison and analysis is that results computed by UGR differed from those of subjective perception, so a corrected UGR formula and corrected glare index and dazzling-sensation was produced. Also, a formula that could represent the state of dazzling-sensation verying with change of luminance was produce.

진균성 부비동염의 임상적 고찰

이승원,김장욱,박영진,송태현,이병돈,장혁순 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

The fungal sinusitis is relatively rare and mainly develops in immunocompromised hosts, but is sporadically appears in the immunocompetent patients, where its incidence tends to be increasing. Because the clinical features of this disease are similar to those of chronic suppurative sinusitis, this entity may be overlooked. We analysed thirteen fungal sinusitis from January 1997 through December 1999 based on clinical features, laboratory characteristics and operative findings. The results are as follows. 1)Fungal sinusitis occurred 3.3 times as frequently in female patients as in male patients and the peak age groups are 4th and 5th decades. 2) The time intervals between the onset of symptoms and the first hospital visit were as follows: 5 cases within 3 months, 1 case between 4 and 6 months, 3 cases between 7 and 12 months, and 4 cases over 1 year. 3) The chief complaints were nasal obstruction, postnasal drip, rhinorrhea, cheek pain, headache, ocular pain, sneezing. 4) The characteristic computerized tomographic findings were hyperattenuated lesions in the maxillary sinus and in the nasal cavity near the natural ostium. 5) The most common pathogen was Aspergillus(92.3%) 6) We performed Caldwell-Luc's operation only in 4 cases, Caldwell-Luc's operation with intranasal ethmoidectomy in 3 cases, Caldwell-Luc's operation with endoscopic sinus surgery in 3 cases and endoscopic sinus sugery only in 3 cases. 7) Most of the gross specimens revealed a fungus ball containing a black-brown necrotic debris or caseous material.

랫드에서 신우황청심원의 급성 및 아급성독성시험

오승민,남혜윤,김준수,연제덕,신대희,이진영,박대규,조명행,정규혁 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

The acute and subacute toxicity of New Woohwangchungsimwon(NWCH) which was used l-muscone as substitutive material of musk were investigated in S.D. rats. In intraperitoneal acute toxicity test, rats(Sprague-Dawley, SPF) were injected intraperitoneally with dosages of 0, 540, 750, 1,070, 1,500 and 3,000 ㎎/㎏. Body weights were significantly decreased at 540 ㎎/㎏ dose group in both sexes and abnormal autopsy findings were founded in both sexes at all treated groups. Intraperitoneal LD_50 of NWCH was 812.3 ㎎/㎏ in male and 872.3 ㎎/㎏ in female rats. In the subacute toxicity study, NWCH was administrated orally to both sexes of rats for 4 weeks as several doses(0, 320, 800 and 2,000 ㎎/㎏). There were neither dead animals nor significant changes of body weights during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, urinalysis, hematology, serum biochemical analysis, and other findings. Above data strongly suggest that no observed adverse effect level of NWCH might be over 2,000 ㎎/㎏/day in this study.

위선암에 병발된 혈액학적 합병증 1예 : 백적아구증, 파종성 혈관내 응고, 미세 맥관성 용혈성 빈혈

정혜경,김희진,이선영,최진혁,이순남,이홍수,정화순,한운섭 梨花女子大學校 醫科大學 醫科學硏究所 1994 EMJ (Ewha medical journal) Vol.17 No.2

Among the hematologic complications of solid tumors, leukoerythroblastosis, DIO and MAHA have been known to be clinically significant According to the recent increasing incidence of cancer, these hematologic complications have been reported frequently. However, the simutaneous developement of such manifestations is rare. Recently, we have experienced a patient with gastric adenocarcinoma in which case severe bleeding tendency were observed and laboratory studies including bone marrow examination demonstrated the findings of leukoerythroblastosis, DIC and MAHA. She died of upper gastrointestinal bleeding despite the supportive care such as vlood transfusions. We should give attention to the findings of peripheral golld smear for the patient with malignancy and it is mandatory for therapeutic plan to perform bone marrow examination if the above mentioned hemorrhagic complications are suspected.

딥 러닝을 이용한 CBCT 영상에서 상악동과 병변 자동 분할 방법

김다엘(Da-El Kim),유연선(Yeon-Sun Yoo),양수(Su Yang),김진(Jin Kim),강세룡(Se-Ryong Kang),천소영(So-Young Chun),유지용(Ji-Yong Yoo),최민혁(Min-Hyuck Choi),노시연(Siyeon Noh),김세훈(Sehum Kim),이원진(Won-Jin Yi) 대한전자공학회 2022 대한전자공학회 학술대회 Vol.2022 No.4

Expression of Ganglioside GT1b in Mouse Embryos at Different Developmental Stages after Cryopreservation

Kim, Bo-Hyun,Jung, Ji-Ung,Ko, Ki-Sung,Kim, Won-Sin,Kim, Sun-Mi,Ryu, Jae-Sung,Jin, Jung-Woo,Yang, Hyo-Jung,Kim, Ji-Su,Kwon, Hyuck-Chan,Nam, Sang-Yoon,Kwak, Dong-Hoon,Park, Yong-Il,Koo, Deog-Bon,Choo, Y 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.1

Gangliosides are a family of sialic acid-containing glycosphingolipids that are abundant in neurons and have a variety of functions in developing and mature tissues. We examined the expression of ganglioside GT1b in the embryonic preimplantation stage after freezing and thawing processes to determine the regulatory roles of ganglioside GT1b in early embryonic development. ICR mouse embryos at the two-cell stage obtained by flushing the oviducts were frozen by two cryopreservation procedures, slow freezing using a programmable freezer or vitrification by direct plunging into liquid nitrogen. Slow freezing was conducted with equilibration in 1.5 M 1,2-propanediol or 5% equilibration glycerol. Vitrification was applied with a 10-15 min equilibration in 7.5% ethylene glycol (EG), 7.5% dimethylsulfoxide (DMSO), and 30 sec in a solution of 15% EG, 15% EMSO and 0.5 M sucrose. Immediately after thawing, the survival rate of the embryos was assessed by their morphology and ability to develop to blastocysts in culture. The survival rate of vitrified and thawed embryos (92%) was significantly higher than that of slow frozen and thawed embryos (76%) (P<0.05). A tendency of higher blastocyst rate was found in the vitrified and thawed embryos compared to that of the slow frozen and thawed embryos. Confocal immunofluorescence staining confirmed that surviving embryos expressed ganglioside GT1b, with the strongest expression at the compacted eight-cell or later stage embryos. Ganglioside GT1b was not observed in the TUNEL-positive, apoptotic embryos, suggesting that cryopreservation had induced DNA breaks in them. These results suggest that ganglioside GT1b may play an important role in embryo survival or development.

Genomic characteristics of triple negative apocrine carcinoma: a comparison to triple negative breast cancer

Kim Ji-Yeon,Park Sabin,Cho Eun Yoon,Lee Jeong Eon,Jung Hae Hyun,Chae Byung Joo,Kim Seok Won,Nam Seok Jin,Cho Soo Youn,Park Yeon Hee,Ahn Jin Seok,Lee Semin,Im Young-Hyuck 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Apocrine carcinoma is a rare breast cancer subtype. As such, the genomic characteristics of apocrine carcinoma with triple negative immunohistochemical results (TNAC), which has been treated as triple negative breast cancer (TNBC), have not been revealed. In this study, we evaluated the genomic characteristics of TNAC compared to TNBC with low Ki-67 (LK-TNBC). In the genetic analysis of 73 TNACs and 32 LK-TNBCs, the most frequently mutated driver gene in TNAC was TP53 (16/56, 28.6%), followed by PIK3CA (9/56, 16.1%), ZNF717 (8/56, 14.3%), and PIK3R1 (6/56, 10.71%). Mutational signature analysis showed enrichment of defective DNA mismatch repair (MMR)-related signatures (SBS6 and SBS21) and the SBS5 signature in TNAC, whereas an APOBEC activity-associated mutational signature (SBS13) was more prominent in LK-TNBC (Student’s t test, p < 0.05). In intrinsic subtyping, 38.4% of TNACs were classified as luminal A, 27.4% as luminal B, 26.0% as HER2-enriched (HER2-E), 2.7% as basal, and 5.5% as normal-like. The basal subtype was the most dominant subtype (43.8%) in LK-TNBC (p < 0.001), followed by luminal B (21.9%), HER2-E (21.9%), and luminal A (12.5%). In the survival analysis, TNAC had a five-year disease-free survival (DFS) rate of 92.2% compared to 59.1% for LK-TNBC (P = 0.001) and a five-year overall survival (OS) rate of 95.3% compared to 74.6% for LK-TNBC (P = 0.0099). TNAC has different genetic characteristics and better survival outcomes than LK-TNBC. In particular, normal-like and luminal A subtypes in TNAC have much better DFS and OS than other intrinsic subtypes. Our findings are expected to impact medical practice for patients diagnosed with TNAC.