치매방지 작업기구 사용이 항산화효소 활성에 미치는 영향

조경혜,박정은,윤지윤,문세보,박효영,정인옥,이정원 서울여자대학교 자연과학연구소 2001 자연과학연구논문집 Vol.13 No.-

We developed an occupational therapy tool to maintain and improve the cognitive function for the elderly who have senile dementia. 86 elderly people took a mininal mental state examination (MMSE) for the therapy to perform the occupational therapy, and divided into three groups based on avarage ages and MMSE values. The occupational therapy was treated three times per week for 10 weeks. The results showed that there were significant differences in the performing scores before and after the treatment ; the scores for the occupational therapy were increased significantly (p<0.001) and the performing time was shortened from 6-week throughout 10-week regimen. This study was to examine the effect on the activity of antioxidant enzymes by occupational therapy. The activities of antioxidant enzymes such as catalase, Glutathion-6-phosphate dehydrogenase (G6PDH), and superoxide dismutase (SOD) were increased to the normal standard by the occupational therapy for the elderly having dementia. The recovery of activities of antioxidant enzymes was obvious in severe demented elderly compared to those in normal or mild demented elderly. These results suggest that the occupational therapy had an beneficial effect on the protection against the oxidative stress especially for severe demented elderly, and the occupational tool need to be modified so as to improve the protection against the oxidative stress for normal and/or mild demented elderly.

생쥐 뇌조직 체외배양법을 활용한 향산화제의 향독성효과에 관한 연구

이수현,박정은,김희옥,서혜원,조경혜 서울여자대학교 자연과학연구소 2000 자연과학연구논문집 Vol.12 No.-

In an effort to establish an in vitro model of Parkinson's disease (PD), we prepared slice cultures of midbrain from newborn mouse. The slice section containing substantia nigral region was cultured for 10days. The striatal culture was grown to preserve the distribution of interneurons with minimal contamination of any other brain tissue. Using this explant culture containing both glial cells and nitro-striatal dopamine neurons, the protective effect of antioxidant pineal hormone and melatonin on 1-methyl-4-phenyl-1, 2,3,6-tetrahydropyridine (MPTP) -induced loss of the dopamine neurons was investigated. MPTP selectively damaged dopaminergic neurons, as indicated by immunoblot and immunohistochemistry for glial fibrillary acidic protein (GFAP) and tyrosine hydroxylase, the marker enzymes for glial and dopaminergic neurons, respectively. Twenty-four-hour treatment of MPTP (50 μM) in the explant culture induced 37% loss of dopaminergic neurons while no apparent damage was observed in glial region. Type B monoamine oxidase (MAO-B) that resides in glial cells, produces free radicals during catecholamine metabolism and also oxidizes MPTP to its active form, MPP+, was activated by 2.2 times after MPTP treatment. Melatonin was able to recover both the dopaminergic neuronal loss and the elevated MAO-B activity level fully to the untreated control level. Similar observation was made bur the co-treatment of vitamin E with MPTP, suggesting the generation of oxygen radical species by MPTP. On behalf of pursuing molecular mechanisms of Parkinsonism, the data illustrate the proposed mechanism of MPTP-induced dopaminergic cell death ; namely, MPP+ that is processed by MAO-B in glial cells is taken up avidly by dopaminergic neurons and causes a toxic effect at least partly, if not mostly, through oxidative stress. The culture system, therefore, can serve as an experimental model of PD in which the putative neuroprotective effects of antioxidants can be investigated. This model is also useful for screening novel drugs and growth factors without a massive sacrifice of animals, and ultimately for developing neuroprotective therapies.

난소 적출 동물모델에서 고흥산 석류 농축액의 갱년기 증상 개선 효과

위지향(Ji-Hyang Wee),정현정(Hyun Jung Jung),정경옥(Kyung Ok Jung),성혜미(Hea Mi Sung),신유림(Yu-Rim Shin),박주현(Ju-Hyun Park),서현영(Hyeon-Young Seo),임정민(Jung-Min Lim),채한정(Han-Jeong Chae),이기웅(Ki Yung Lee) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.4

본 연구는 난소 적출 동물 모델을 이용하여 고흥산 석류 농축액 시험군과 함께 이란산 석류 농축액을 주성분으로 하는 시판 제품의 혈청 생화학적 지표, 골밀도 등 몇 가지 지표를 분석하여 갱년기 증상 개선 효과를 비교하였다. 고흥산 석류 농축액과 시판 제품은 난소 절제 동물 모델의 골밀도(bone mineral density, BMD) 및 percent bone volume(bone volume/tissue volume), bone surface density(bone surface/tissue volume)를 증가시켰으며, 특히 고용량의 고흥산 석류 농축액(PE3)은 통계적으로 유의하게 골밀도 및 골볼륨을 증가시켰다. 시판 제품을 투여한 군도 골밀도를 증가시키는 양상을 보였으나 통계적 유의성은 없어 고흥산 석류 농축액이 이란산 석류 제품보다 골밀도 등 뼈 건강에 관련한 갱년기 증상 개선에 더 효과적이라고 사료된다. 고흥산 석류 농축액은 난소 절제에 의한 체중 증가 및 복부/내장지방 축적을 개선하였으며, 농도 의존적으로 HDL-C(high density lipoprotein cholesterol)는 증가시키고 low density lipoprotein cholesterol은 감소시켰다. 또한 고농도 석류 농축액(PE3)의 HDL-C/total cholesterol이 통계적으로 유의하게 증가하여 고흥산 석류 농축액이 갱년기 여성의 비만 및 혈중지질을 개선할 수 있을 것으로 기대된다. 불안장애 실험에서는 고흥산 석류 농축액 투여로 인해 불안장애가 개선되는 경향을 보였으나 통계적 유의성이 없었고, 이란산 시판제품 투여 시에는 대조군에 비해 유의하게 개선시키는 것으로 나타났다. 우울증 또한 시판 제품 투여군에서는 대조군에 비해 유의하게 개선 효과를 나타내었으나 고흥산 석류 농축액 투여군에서는 우울증 개선 효과가 확인되지 않았다. 이상과 같은 결과를 통해 고흥산 석류 농축액은 난소를 적출한 rat의 골 소실을 현저히 개선하고 복부/내장지방 및 혈중지질을 농도 의존적으로 개선하여 골다공증, 비만 및 심혈관 질환과 같은 갱년기 증상에 효과가 있음을 확인하였다. 그러나 고흥산 석류 농축액 투여군에서 대표적인 갱년기 증상인 우울증과 불안장애 개선에 유의성 있는 효과가 나타나지 않는 것은 동물실험 모델에서 사람에게 나타나는 다양한 갱년기 증상을 모두 평가하기에 한계가 있는 바, 향후 추가적인 연구를 통해 고흥산 석류 농축액이 갱년기 여성에게 나타나는 여러 가지 증상 중 특정 증상에 대해 선택적으로 효과를 나타내는지에 대해 임상시험을 통해 검증할 필요가 있겠다. In this study, the effect of Goheung pomegranate extract on postmenopausal syndrome was evaluated both in vitro and in vivo in ovariectomized Sprague-Dawly (SD) rats. Sixty female SD rats were divided into six groups: sham, sham operation and distilled water; OVX, ovariectomized and distilled water; PE1, ovariectomized and pomegranate concentrate (0.75 mL/twice/d); PE2, ovariectomized and pomegranate concentrate (1.5 mL/twice/d); PE3, ovariectomized and pomegranate concentrate (2.2 mL/twice/d); and CE, ovariectomized and commercial pomegranate concentrate (2.2 mL/twice/d). Percent bone volume (bone volume/tissue volume) and trabecular thickness (Tb.Th) improved in a dose-independent manner in PE1, 2, and 3. Especially, bone mineral density was significantly improved in PE3 (P<0.05) compared to OVX. Pomegranate extract reduced body weight and visceral fat mass. High density lipoprotein cholesterol (HDL-C) level slightly increased in a dose-independent manner in the experimental group. In addition, HDL-C/total cholesterol level of PE3 significantly increased (P<0.05) compared with OVX. These results show that pomegranate concentrate improved blood lipid levels and bone metabolism in ovariectomized rats. Therefore, Goheung pomegranate concentrates are expected to improve cardiovascular and bone-related diseases in menopausal women.

Case Reports : Diagnosis and Symptomatic Treatment of Early Reactive Cardiac Amyloidosis in Systemic Sclerosis

( Rae Seok Lee ),( Hyun Jin Sung ),( Jung Im Jung ),( Hea Ok Jung ),( Seung Min Jung ),( Jennifer Jooha Lee ),( Seung Ki Kwok ),( Ji Hyeon Ju ),( Sung Hwan Park ) 대한류마티스학회 2015 대한류마티스학회지 Vol.22 No.2

Systemic sclerosis as a connective tissue disease could affect all internal organs of the body and could also manifest as a cutaneous lesion. Cardiac involvement leading to cardiac manifestations in systemic sclerosis patients is not rare. However, cardiac amyloidosis combined with systemic sclerosis is extremely rare. Although there were no definite treatment options in this case, symptomatic treatment is the cornerstone of the management plan. In this case report, we described a correct diagnosis and symptomatic medical care of early reactive cardiac amyloidosis with systemic sclerosis and summarize the current state of the relevant literature. (J Rheum Dis 2015;22:132-136)

Deceased donor liver transplantation under the Korean model for end-stage liver disease score-based liver allocation system: 2-year allocation results at a high-volume transplantation center

Hea-Seon Ha,Jung-Ja Hong,In Ok Kim, M.Sc.,Sae-Rom Lee,Ah-Young Lee,Tae-Yong Ha,Gi Won Song,Dong-Hwan Jung,Gil-Chun Park,Chul-Soo Ahn,Deok-Bog Moon,Ki Hun Kim,Sung Gyu Lee,Shin Hwang 대한이식학회 2019 Korean Journal of Transplantation Vol.33 No.4

Background: The Korean model for end-stage liver disease (MELD) score-based liver allocation system was started in June 2016 in Korea. Methods: This study analyzed the detailed allocation results of deceased donor liver transplantation (DDLT) during the first 2 years after the MELD score-based liver allocation system implementation at a high-volume liver transplantation (LT) center in Korea. Results: This study included 174 patients with age above 12 years. The patient ABO blood groups were A (n=65, 37.4%), B (n=51, 29.3%), O (n=28, 16.1%), and AB (n=30, 17.2%). The LT types were primary LT in 141 patients (81.0%) and retransplantation in 33 (19.0%). The Korean Network for Organ Sharing status categories at LT were as follows: status 1 (n=11, 6.3%), status 2 (n=82, 47.1%), status 3 (n=63, 36.2%), and status 4 (n=18, 10.3%). The mean MELD score at LT and waiting period were 36.6±4.6 and 62.1±98.2 days in blood group A; 37.6±3.6 and 25.7±38.1 days in blood group B; 38.8±2.7 and 26.0±30.5 days in blood group O; and 34.8±5.5 and 68.4±110.5 days in blood group AB (P<0.001 and P=0.012), respectively. Patients with blood group O and AB had the highest and lowest mean MELD scores at LT allocation, respectively. Conclusions: Serious deceased organ donor shortage resulted in very high MELD score cutoffs for DDLT allocation. Additionally, a significant inequality was observed in the possibility for DDLT according to blood group compatibility. Nationwide follow-up studies are necessary to precisely determine the allocation status of DDLT.

Poster Session 3 : Metabolism and Enzymology Apoptosis and Cell Cycle Neurobiology ; The Effect of Bisphenol A on Skeletal Muscle of ICR Male Mice

( Jung Won Lee ),( Hyo Young Park ),( In Ok Jung ),( Ji Youn Youn ),( Keum Hwa Choi ),( Moon Jong Boo ),( Kyung Hea Cho ) 한국생화학분자생물학회 (구 한국생화학회) 2002 추계학술대회집 Vol.2002 No.-

혈청 Ferritin 측정에 있어서 Nephelometry 법과 RIA 법의 비교

정옥주,박수현,정보경,박혜란,이종영 대한임상병리학회 1996 대한임상병리학회지 Vol.16 No.6

IgA Levels Are Associated with Coronary Artery Lesions in Kawasaki Disease

Jae-Jung Kim,Hea-Ji Kim,Jeong Jin Yu,Sin Weon Yun,Kyung-Yil Lee,Kyung Lim Yoon,Hong-Ryang Kil,Gi Beom Kim,Myung-Ki Han,Min Seob Song,이형두,Hyun Ok Jun,Kee Soo Ha,Young Mi Hong,장기영,Jong-Keuk Lee 대한심장학회 2021 Korean Circulation Journal Vol.51 No.3

Background and Objectives: Kawasaki disease (KD) is an acute systemic vasculitis that affects the coronary arteries. Abnormal immune reactions are thought to contribute to disease pathogenesis. The effect of immunoglobulin (Ig) isotype (IgG, IgA, IgM, and IgE) on inflammatory data and clinical outcomes of patients with KD was examined. Methods: Ig levels in 241 patients with KD were measured during the acute, subacute, convalescent, and normal phases of the disease. Results: Compared with reference Ig values, IgG, IgA, and IgM levels were significantly higher in the subacute phase, while IgE levels were elevated in 73.9% (178/241) of patients with KD in all clinical phases. However, high IgE levels were not associated with clinical outcomes, including intravenous immunoglobulin unresponsiveness and coronary artery lesions (CALs). Significantly more CALs were observed in the high IgA group than in the normal IgA group (44.7% vs. 20.8%, respectively; p<0.01). In addition, IgA levels in the acute phase (p=0.038) were 2.2-fold higher, and those in the subacute phase were 1.7-fold higher (p <0.001), in the CAL group than in the non-CAL group. IgA concentrations increased along with the size of the coronary artery aneurysm (p <0.001). Furthermore, there was a strong correlation between IgA levels and CAL size (r=0.435, p<0.001), with a high odds ratio of 2.58 (p=0.022). Conclusions: High IgA levels in patients with KD are prognostic for the risk of CALs.

청각장애대학생의 심리상담 경험에 대한 내용의 질적 분석

정혜인 ( Hea In Jung ),정옥경 ( Ok Kyung Jeong ) 한국특수교육문제연구소 2009 특수교육저널 : 이론과 실천 Vol.10 No.4

본 연구는 수화통역사를 대동한 청각장애대학생과의 상담에서 청각장애대학생들이 상담경험을 어떻게 하고 있는지 이해하고자 하였다. A시 소재 대학에서 개인 상담과 집단 상담에 참여한 10명의 상담과정을 녹음한 축어록 자료를 근거로 합의적 질적 분석(CQR) 방법을 사용하여 분석하였다. 분석결과 청각장애대학생이 상담의 초기과정에서는 이전에 하지 못했던경험, 정보전달과는 다른 느낌, 문제를 보는 관점의 새로움과 신기함, 부끄러움과 당황스러움, 의사소통에 답답함과 오해 등을 보고하였다. 상담자와 수화통역사에 관련한 것 중 상담자는 상담자의 전문성, 상담자에 대한 신뢰, 문제해결 방법의 상이성, 상담자의 경청과 질문,눈 맞춤, 기다리기, 새로운 시각의 제시 등을 들었으며 수화통역사는 비밀에 대한 염려, 빠른의사전달, 친근감 등의 요인을 들었다. 상담의 말기과정에서는 처음과 비교한 문제의 해결정도를 어느 정도까지 되었다고 생각하고 있으며, 상담을 통해서 어떠한 기대를 하는가에 관련하여서는 나의 문제를 털어놓은 것만으로도 속이 시원하다는 보고가 가장 많았으며 처음에 생각했던 것과는 다른 해결책을 얻게 됨, 상담이 마무리가 되어가자 신기하게도 문제가 자연스럽게 해결됨을 느낌, 아무리 표현해도 아무도 알지 못할 것이라 생각 등이 있었다. In this study, the counselling processes of 10 students of a university locate in A City who participated in personal counselling and group counselling were analyzed using Consensual Qualitative Research (CQR) based on the recorded word-for-word records to understand how the people with hearing impairment are experiencing their counselling in their counselling sessions accompanied by sign language interpreter. In the analysis result, people with hearing impairment reported in their early process of counselling the experiences they hadn’t experienced before, different feel than information delivery, newness and novelty of the view of problems, humiliation and consternation and their frustration and misunderstanding in communications. It can be said that the experience of counselling for people with hearing impairment who are less exposed to counselling is an experience of new culture.

Capsaicin effects on brain - derived neurotrophic factor in rat dorsal root ganglia and spinal cord

(Sun Ok Ha),(Hea Jin Yoo),(So Yun Park),(Hae Sook Hong),(Dong Sun Kim),(Hee Jung Cho) 경북대학교 유전공학연구소 2000 遺傳工學硏究所報 Vol.14 No.-