http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
이화운,김유근,정우식,오은주,임헌호,노순아,반수진,최현정,김민선,김헌숙 부산대학교 환경문제연구소 2002 環境硏究報 Vol.20 No.-
In the view of the results investigated the effect of air pollutants that is emitted from a new generator facilities, the total estimated concentration which is the summation of a contributional concentration and observational concentration is much less than that of atmospheric environment criterion for the air pollutants. So we can predict that the operation of the new generator facilities with mitigation equipment don't seriously affect around environment. At the same time, we compared the contributional concentration of before mitigation equipment with that of after it to examine the decreasing amount due to mitigation equipment. The result showed that the concentration of SO2, NO2 and PM-10 is reduced to 25%, 30% and 26% of original value, respectively.
In vitro and in vivo Activities of SM-101, a Micture of Metampicillin and Sulbactam
Choi, Keum-Hwa,Kim, Sook-Kyung,Baek, Moon-Chang,Kim, Byong-Kak,Lee, Dong-Young,Choi, Eung-Chil The Pharmaceutical Society of Korea 1995 Archives of Pharmacal Research Vol.18 No.6
SM-101 is a mixture of metampicillin and sulbactam(2:1). The antibacterial activities of SM-101 were compared with those of metampicillin, piperacillin and Augmentin. It showed powerful antibacterial activities against major strains. Except P. anruginosa and S. marcescens, the in vitro antibacterial activity of SM-101 was higher than those of metampicillin, piperacillin and Augmentin against Staphylococcus spp., Streptococcus spp., Moganella morganii, E. Coil, and Proteus spp. The $ED_{50}$ values of SM-101 were two-fold or greater than those of metampicillin, piperacillin and Augmentin against $\beta-lactamase$ producing strains, p. mirabilis GN79 and M. morganiii MB4-11. The in vivo efficacy of SM-101 was more active than metampicillin and pipeeracillin and similar to Augmentin against S. aureus Smith, E coli MB4-01 and K. pneumoniae MB4-02.
Resistance Mechanism of Acinetobacter spp. Strains Resistant to DW-116, a New Quinolone
Choi, Keum-Hwa,Baek, Moon-Chang,Kim, Byong-Kak,Choi, Eung-Chil The Pharmaceutical Society of Korea 1998 Archives of Pharmacal Research Vol.21 No.3
DW-116 is a new fluoroquinolone antimicrobial agent with a broad spectrum. In order to elucidate the resistance mechanism to DW-116 in Acinetobacter spp. bacteria, total chromosomal DNA was isolated from 10 strains of Acinetobacter spp. resistant to DW-116. Quinolone resistance determinant region (QRDR) of DNA gyrase gene was amplified by PCR. The 345 bp nucleotide fragment yielded was inserted into pKF 3 which was used as the vector. Comparisons of the DNA sequences of 8 strains with that of the wild type strain revealed a Ser-83 to Leu mutation in mutants and all ten strains contained one silent mutation$(T{\rightarrow}G)$in QRDR. From Acinetobacter MB4-8 strain, DNA gyrase was isolated and purified, through novobiocin-sepharose, heparin-sepharose affinity column chromatography. The enzyme was composed of two subunits and the molecular mass of subunits A and B were 75.6 and 51.9 kDa, respectively. The supercoiling activity of the reconstituted DNA gyrase composed of subunit A from Acinetobacter MB4-8 and subunit B from E. coli was not inhibited by $128{\mu}\textrm{g}$ml of ciprofloxacin. It might be said that one of the resistance mechanisms to DW-116 in Acinetohacter MB4-8 was subunit A alteration of DNA gyrase.
Generation of transposon insertion mutants from type A Pasteurella multocida
Choi, Keum-hwa,Maheswaran, Samuel K. The Korean Society of Veterinary Science 1999 大韓獸醫學會誌 Vol.39 No.2
The transposon TnphoA was used to generate avirulent mutants from a type A Pasteurella multocida. A suicide vector plasmid pRT733 carrying TnphoA, having the kanamycin resistant gene and harbored in Escherichia coli K-12 strain SM10(${\lambda}pir$), was mated with streptomycin resistant P. multocida P-1059 strain as recipient. This resulted in the generation of two TnphoA insertion mutants (transconjugants, tc95-a and tc95-b) which were resistant both to kanamycin ($Km^{R}$) and streptomycin ($Sm^{R}$), secreted alkaline phosphatase, and were avirulent to turkeys. Southern blot hybridization using two probes derived from internal fragments of TnphoA, confirmed the insertion of TnphoA into 12.9kb or 13.7kb DNA fragment from the EcoRV digested genomic fragments of transconjugants. The two transconjugants, tc95-a and tc95-b, were distinguishable from their parent strains by differences in ribotypes, and outer membrane protein profiles. TnphoA insertion in both transconjugants also resulted in constitutive expression of a 33Kd iron regulated outer membrane protein (IROMP). The gene encoding $Sm^{R}$ was also located within the same 12.9kb EcoRV genomic fragment from both transconjugants. Furthermore, our finding that the recipient P. multocida P-1059 $Sm^{R}$ strain and both transconjugants were avirulent to turkeys suggest that the either 12.9kb or 13.7kb genomic DNA contains the virulence gene and speculate that the presence of $Sm^{R}$ gene or TnphoA insertion may be responsible for regulating and inactivating the gene(s) encoding virulence in P. multocida.
최금화(Keum Hwa Choi),오태권(Tae Gwon Oh),권애란(Ae Ran Kwon),김병각(Byong Kak Kim),최응칠(Eung Chil Choi) 대한약학회 1999 약학회지 Vol.43 No.1
The bactericidal activities of DW-116, a new fluoroquinolone was estimated by comparing the minimal bactericidal concentrations (MBCs) of it against some Gram-positive and -negative bacterial concentration with the minimal inhibitory concentrations (MICs). The MBCs against the test organisms were equal to or two times higher than MICs. The results support that the antibacterial activity of DW-116 is bactericidal.
새로운 퀴놀론 항균제 DW-116의 Post-Antibiotic Effect
최금화(Keum Hwa Choi),오태권(Tae Kwon Oh),백문창(Moon Chang Baek),김병각(Byung Kak Kim),최응칠(Eung Chil Choi) 대한약학회 1998 약학회지 Vol.42 No.2
The post-antibiotic effects (PAE) of DW-116 were evaluated against Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 27348, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa MB4-16, repectively. Against gram-positive bacteria, PAEs of DW-116 were longer duration (20-35 min) than those of rufloxacin(1O-20 min), and shorter than those of ciprofloxacin (50-90 min). Especially, against E. coli, DW-116 and ciprofloxacin obtained approximately 3 hrs of PAES.
( Hwa Young Lee ),( Se Won Kim ),( Joon Young Choi ),( Jae Hee Chung ),( Mi Ra Han ),( Yong Suk Lee ),( Eun Hyoung Kang ),( Keum Sook Jeun ),( Chin Kook Rhee ),( Young Kyoon Kim ),( Seok Chan Kim ) 대한결핵 및 호흡기학회 2015 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.120 No.-
Background: The purpose of the study is to evaluate the impact of rapid response team (RRT) activity during the post-operative period. Methods: From Mar 2014 to Dec 2014, a total of 1171 patients were electronically screened during the post-operative period for at least 24 hours in departrment of orthopedic surgery, St. Mary`s hospital. Among them, 1129 were discharged without serious adverse events (SAEs), 30 were activated by requests of attending doctors or nurses or the electronic medical alert system and 12 had SAE without RRT activation. We compared the clinical characteristics and progress of 30 activated and 12 non-activated patients by retrospective chart review. Results: The most common SAE was pulmonologic origin in activated patients. Mean modified early warning scores (MEWS) at admission and post-operative day 1 (POD 1) were higher in activated patients (2.3 vs. 1.08, P = 0.009 / 2.4 vs. 0.8, P = 0.000), but APACHE II score at POD 1 were not statistically different (8.4 vs. 10.5, P = 0.146). The percentage of CPR and mortality were higher in non-activated group (13.3% vs. 25%, P = 0.063, 10% vs. 41.7%, P = 0.031). Conclusion: At-risk patients with higher MEWS at admission and POD1 were prone to activate RRT and be undertaken successful intervention during the postoperative period. But the patients who were not detected by electronic medical alert system or attending physician showed higher rate of CPR and mortality. Further development of postoperative screening system would be needed.