자궁경부암에서 혈관 내피 성장인자 ( VEGF ) 및 VEGF Mrna 의 발현에 대한 연구

이선경(Seon Kyung Lee),김승보(Seung Bo Kim),지성길(Sung Gil Chi),염윤석(Yoon Seok Yum),이주희(Ju Hee Lee) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.1

N/A Objective : Angiogenesis, the formation of blood vessels by sprouting from pre-existing ones, is essential for the growth of solid tumors beyond 2~3mm in diameter and for tumor metastasis. Vascular endothelial growth factor (VEGF), is known as vascular permeability factor(VPF) and mediates vascularization and tumor-induced angiogenesis. This study examined the potential of growth, invasion, and metastasis of uterine cervical carcinomas associated with neovascularization. Methods : From January 1996 to December 1999, at the Department of Obstetrics and Gynecology, Kyung-Hee University Hospital, 37 uterine cervical carcinomas and 7 normal cervical tissues were obtained and the samples were immediately frozen and stored at -70℃. Immunohistochemical staining for VEGF was carried out to study VEGF localization, and the levels of VEGF subtype mRNAs were determined by quantitative RT-PCR in specimens. The relation between VEGF subtypes expression of cervical cancers was analysed. Results : The positive staining for VEGF is seen dominantly in the cytoplasm of the cancer cells, and faintly in interstitial cells. The intensity of staining was stronger in squamous carcinomas than in adenocrcinomas, but there was no significant difference (p>0.05). Quantitative RT-PCR analysis demonstrated significantly increased VEGF121/VEGF165 mRNA expression levels (>0.56/>0.72) in 21 (56.8%) and 15 (40.5%) of 37 cervical carcinomas comparing to control groups (mean: 0.28/0.36). There was no obvious relationship between VEGF121/VEGF165 mRNA expression levels and the clinical parameters examined including age, pathology, differentiation, tumor size, lymphovascular space invasion, LN involvement and invasion depth except clinical stage (p<0.05). Conclusions : The overexpression of VEGF mRNA may be an important contributing factor in cervical carcinomas. There is no significant differenece of VEGF mRNAs levels according to clinical parameters, so it seems that the expression of VEGF is involved in the promotion of angiogenesis on cervical cancer and plays an important role in early invasion.

Immunoblot 법을 이용한 간흡충항원(肝吸蟲抗原)의 발육단계별(發育段階別) 항원성분석(抗原性分析)에 관한 연구(硏究)

이선경,주경환,정명숙,임한종,Lee, Seon-Kyung,Joo, Kyoung-Hwan,Chung, Myung-Sook,Rim, Han-Jong 한국농촌의학지역보건학회 1991 농촌의학·지역보건 Vol.16 No.1

Serodiagnosis of Clonorchis sinensis infections will probably be a first choice tool for screening of clonorchiasis in a future because of increasing difficulties in collection and examination of stools. The sensitive test such as ELISA can he used effectively. However there are some limitations in serological diagnosis for the detection of serum antibody. One of the major problems is the non-specificity of the antigens which produce cross reaction with other helminthic infection sera. To solve this problem. many investigators have tried to purify the antigens used. In this study, we determined the antigenic profile of the crude saline extract antigen of C. sinensis at early developmental stage based on SDS-PAGE and immunoblotting techniques for the purpose of understanding the nature of C. sinensis worm antigen The following results were obtained : 1) The SDS-PAGE showed many protein hands ranging from 10Kd to 91Kd relative molecular weight. Among them, 66, 46, 40, 33, 27, 24, 16, 14 and 10Kd bands were observed as a principle bands. The protein components of C. sinensis changed chronologically during their early developmental period. 44Kd band was stained unclearly in antigen of 2 weeks worm, but changed to concentrated state in antigen of 5 weeks worm. 35Kd band was found in antigen of 2 weeks worm, however this band was disappeared in antigen of 5 weeks worm. 22Kd band also lost its staining property gradually. 2) In spite of differences in antigenic profile, there was no differences in the data obtained by microplate ELISA using each antigen preparation. Absorbance value began to rise in between 2 to 3 weeks after infection. 3) By EITB. serum antibody recognized major protein bands with molecular weight of 91, 85, 63, 46, 40, 33, 24, 14 and 10Kd hand respectively. Among them 66, 33, 17 and 14Kd bands were observed as non-specific band because they reacted even in normal control sera. Generally, gradual increase of positive reactions were observed as the infection period of C. sinensis was prolonged. In other hand, the reaction of 10Kd hand did not occurred when 26th week sera was tested. 4) The positive reactions using antigens of 2 weeks worm, especially on 40 and 24Kd bands, were most strong and sharply demarcated compared to those of 3~5 weeks worm antigen.

자궁경부암 조직에서의 Telomerase 활성도와 hTERT mRNA 발현 HPV E6 검출의 상관관계에 관한 연구

홍종우(Jong Woo Hong),이선경(Seon Kyung Lee),반태학(Tae Hak Ban),지성길(Sung Gil Ji) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.3

N/A Objective: Telomerase is a ribonucleoprotein enzyme which stabilizes chromosomal structure, thereby inducing cellular immortality. We investigated telomerase activity and human telomerase reverse transcriptase (hTERT) mRNA expression in relation to high-risk human papillomavirus (HPV) DNA presence in cervical carcinomas. Met hods: From December 1995 to December 1999, at the department of obstetrics and Gynecology of Kyung-Hee University Hospital,32 cervical carcinomas and 5 corresponding nontumor cervical tissues were obtained and the samples were immediately frozen and stored at -70℃. Telomerase activity was measured by using telomerase PCR ELISA, a modified version of the TRAP. Analysis of the expression of hTERT mRNA was performed by quantitative RT-PCR and the analysis of the HPV E6 gene was performed by DNA-PCR. Result: All of the carcinomas examined exhibited strongly positive for telomerase activity (OD>0.24), whereas telomerase activity was week or not found in the 5 corresponding nontumor cervical tissues. Quantitative RT-PCR analysis demonstrated significantly increased hTERT mRNA expression levels (>0.024) in most carcinomas comparing to control groups. There was no obvious relationship between telomerase activity levels and the clinical parameters examined including age, clinical stage, pathology, differentiation, tumour size, LN involvement and invasion depth except lymphovascular space invasion (p=0.03). In the correlation between the levels of hTERT mRNA expression and telomerase activity, correlation index which was 0.916,shows high correlation (p=0.01). According to the analysis of HPV E6 gene,29 of 32 (90.6%)carcinomas showed HPV E6 positivity. Conclusion: There is a strong association between telomerase activity and hTERT mRNA expression, and up-regulation of hTERT probably plays a role in the progression of cervical carcinomas. Telomerase is at least partially activated by viral oncogenes of high-risk types. There is no obvious relationship between telomerase activity levels and the clinical parameters except LSVI (p=0.03). These findings provides that telomerase may play an important role in the early stage of carcinogenesis.

부인암에서의 Telomerase 활성도와 자궁경부암 조직에서의 HPV E6 의 발현과 Telomerase 활성도의 상관관계

유용신(Yong Sin You),이선경(Seon Kyung Lee),김승보(Seung Bo Kim),지성길(Sung Gil Chi) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.8

N/A Object : In this study, to evaluate the putative role of telomerase in gynecologic malignancies (cervical ca, ovarian ca, endometrial ca), we measured telomerase activity in malignant gynecologic tumor tissues and normal tussues, and compared it with prognostic factors in cervical cancer. To evaluate the correlation of telomerase activity and human papillomavirus (HPV) infection in cervical cancer, the analysis of HPV E6 gene was performed. Method : Specimens were obtained from 51 women who underwent gynecologic radical operation and 13 normal tissues (from December 1995 to December 1996) in the Department of Obstetrics and Gynecology, Kyung-Hee Univ. Medical Center. With Telomerase PCR ELISA (Boehring Mannheim), modified TRAP (Telomere Repeat Amplication Protocol), we examined telomerase activity of 32 cervical carcinomas, 11 ovarian carcinomas, 8 endometrial carcinomas, 5 normal cervical tissues, 4 normal ovarian tissues and 4 normal endometrial tissues. The analysis of HPV E6 gene was performed by PCR amplication. We compared the abnormally high telomerase activity with prognostic factors, also compared the telomerase activity with the expression of HPV E6 gene in cervical cancer tissues. Result : We detected the abnormally high telomerase activity in all cervical carcinomas, 10 of 11 (90.9%) ovarian carcinomas, 6 of 8 (75.0%) endometrial carcinomas, but couldn't detect in each normal tissues. There was statistically no significant difference of telomerase activity levels according to age, clinical stage, pathology, differentiation, LN involvement, depth of invasion and tumor size except lymphovascular space invasion in cervical carcinomas (p<0.05). According to the analysis of HPV E6 gene, 29 of 32 (90.6%) in 32 cervical cancer tissues showed HPV E6 positivity. So it was considered that telomerase activation was closely related with the expression of HPV E6 gene. Conclusion : Telomerase activation is associated with immortalization or malignant transformation of gynecologic cancers. The expression of HPV E6 gene is considered to activate telomerase in cervical cancer.

선행 제왕절개술 후 질식 분만: 성공의 예측인자들

동서연 ( Seo Yun Tong ),김여홍 ( Yeo Hong Kim ),최영준 ( Young Jun Choi ),김소라 ( So Ra Kim ),이보연 ( Bo Yon Lee ),이선경 ( Seon Kyung Lee ),허주엽 ( Chu Yeop Huh ),김승보 ( Seong Bo Kim ) 대한주산의학회 2003 Perinatology Vol.14 No.4

목적: 선행제왕절개 후 질식분만을 시도한 산모들 중 성공군과 실패군을 비교하여 성공을 예측할 수 있는 인자를 알아보고자 하였다. 방법: 1997년 3월부터 2003년 2월까지 경희대학교 의과대학 산부인과에서 선행제왕절개 후 질식분만을 위하여 내원한 환자중 질식분만의 금기가 아닌 경우 위험요소 등에 대하여 충분히 상의한 후 분만을 시도한 382명의 환자를 대상으로 하였다. 대상군의 의무기록을 통하여 분만당시 산모의 나이, 분만력, 제태연령, 신생아 몸무게, 입원시 자궁경부 상태 및 Bishop score, 분만의 진행과정, 결과 및 합병증을 조사하여 성공군과 실패군의 차이를 비교분석 하였다. 결과: 292(76.5%)명이 질식분만에 성공하였다. 여러 인자 중 태아의 몸무게(odds ratio, 0.2 ; 95% confidence interval (CI), 0.1-0.4), 입원시 Bishop score(odds ratio, 1.8 ; 95% CI, 1.5-2.1), 질식분만의 과거력(odds ratio, 3.6 ; 95% CI, 1.1-11.6)이 VBAC의 성공과 관련이 있었다. 또한 선행제왕절개시 적응증이 태위이상인 경우에 성공률이 높은 것으로 나타났다(odds ratio, 3.2 ; 95% CI, 1.2~8.6). 입원시 Bishop score로 VBAC의 성공을 예측할 수 있는 지 알아보기 위하여 receiver-operating characteristic curve 분석을 시행하였고, 5점을 cut-off로 하였을 때 47.4%의 민감도와 87.6%의 특이도를 보였다(AUC, 0.765 ; p<0.05). 결론: 출생체중, 질식분만의 과거력, 입원시 Bishop score가 선행제왕절개 후 질식분만을 고려하는 경우 성공의 예측인자로서 유용하게 사용되어 질 수 있을 것이다. Objective: The purpose of this report is to determine the predictable factors for successful vaginal birth after cesarean (VBAC). Methods: In this study, 382 women who attempted VBAC at Kyung-Hee university hospital were included. The medical records of them were reviewed retrospectively. Informations was collected about demographics, medical history, obstetric history, neonatal birth weight, complications, treatment, and outcome of the index pregnancy. Results: The overall success rate was 76.5% (292 cases). Among variables, neonatal birth weight (odds ratio, 0.2; 95% confidence interval, 0.1-0.4), Bishop score at admission (odds ratio, 1.8; 95% confidence interval, 1.5-2.1 ; p<0.05), and history of vaginal delivery (odds ratio, 3.6 ; 95% confidence interval, 1.1-11.6 ; p<0.05) were significant. The Bishop score showed most significant relationship with successful VBAC. The Bishop score 5 at admission had a sensitivity of 47.4% and a specificity of 87.6% in the prediction of successful VBAC. Malpresentation as indications of previous cesarean delivery showed more tendency to succeed than others. Conclusion: Several factors such as birth weight, Bishop score at admission, and history of vaginal delivery may be used to predict the success of VBAC.

케라틴/PLGA 복합체 필름의 적층 원통형 지지체가 섬유륜 재생에 미치는 영향

이선경(Seon Kyoung Lee),홍희경(Hee Kyung Hong),김수진(Su Jin Kim),김용기(Yong Ki Kim),이동원(Dong Won Lee),강길선(Gil Son Khang) 한국고분자학회 2010 폴리머 Vol.34 No.5

조직공학에 이용되는 고분자인 poly(lactic-co-glycolic acid) (PLGA)는 높은 생체적합성 및 생분해성의 장점을 지니지만, 생체활성물질의 결여와 소수성으로 인해 세포의 부착에 어려움을 가진다. 또한 PLGA의 가수분해 과정 중 생성되는 산 분해물이 조직주변의 pH를 감소시켜 염증을 유발하는 단점을 가지고 있다. 따라서 이러한 단점을 보완하기 위한 방법으로 천연고분자인 케라틴을 첨가한 PLGA필름을 제작하였으며 조직공학적 디스크재생에 응용하기 위하여 케라틴/PLGA 복합체필름의 원통형 지지체를 제작하였다. 이 원통형 지지체의 내부는 케라틴/PLGA 필름을 말아서 사용하였으며 외부는 PLGA 다공성 지지체 링을 제작하여 사용하였다. 본 연구에서는 이 지지체에 섬유륜세포를 파종하여 PLGA 필름과 케라틴/PLGA 필름을 이용한 원통형 지지체에서의 세포의 부착 및 생존율을 비교하고 케라틴을 첨가한 지지체의 우수성을 확인하였으며 강도의 우수성 또한 확언하였다. 이 결과는 디스크재생을 위한 지지체의 연구에 유용한 정보를 제공할 것이다. We developed laminated cylindrical scaffolds composed of poly(lactide-co-glycolide) (PLGA) and keratin, and investigated their potential for tissue engineering and disk regeneration. The scaffold was designed to have two parts, i.e. inner cylinder and outer disk, to mimic a natural disk. The outer disk was composed of PLGA and the inner cylinder was prepared using PLGA film or PLGA/keratin hybrid film. In this study, we investigated the effects of keratin on the growth and proliferation of annulus fibrous(AF) cells in the cylindrical scaffolds. Scaffolds containing PLGA/keratin films showed a significantly higher cell proliferation and expression of collagen I and II than the counterpart with PLGA films. Keratin containing scaffolds also exhibited an excellent mechanical strength, demonstrating that keratin influences the proliferation of annulus fibrous cells. The results provide valuable information on PLGA/keratin films for tissue engineered disk regeneration.

유아교사의 사회적 지지 및 직무스트레스가 교수몰입도에 미치는 영향

이선경(Lee Seon Kyung),송미정(Song Mi Jeoung),온은아(On Eun A) 한국유아교육·보육복지학회 2016 유아교육·보육복지연구 Vol.20 No.1

The purpose of this study was to reveal the relationship between early childhood teachers’ social support, job stress, and teaching flow. We applied three rating scale tools to the subjects of this research, 209 early childhood teachers in Jeonra Buk province, in order to measure their social support, job stress, and teaching flow. The results of this study are as follows. First, significantly high correlation exists between social support, job stress and teaching flow. The higher social support and the lower job stress early childhood teachers have, the higher teaching flow is observed. Second, early childhood teachers' teaching flow is influenced by emotional support and appraisal support in that order. Early childhood teachers' job stress has comparatively slight influence on teaching flow. Further studies on the correlation between these two factors is needed. These results are expected to be used as baseline data for increasing early childhood teachers' teaching flow and also show that high teaching flow may improve the quality of early childhood teachers' life and then contribute toward their improved professionalism. 본 연구는 유아교사의 사회적 지지 및 직무스트레스와 교수몰입도의 관계는 어떠하며, 사회적 지지 및 직무스트레스가 교수몰입도에 미치는 영향력은 어떠한지 밝히는 것을 목적으로 한다. 이를 위해 전북지역 유아교사 209명을 대상으로 사회적지지, 직무스트레스, 교수몰입도에 대한 평정척도를 활용하였다. 본 연구의 결과는 다음과 같다. 첫째, 유아교사의 사회적 지지와 직무스트레스 및 교수몰입도 간에는 유의미하게 높은 상관관계가 나타났다. 즉, 유아교사의 사회적 지지가 높을수록, 직무스트레스가 적을수록 교수몰입도가 높은 것으로 나타났다. 둘째, 유아교사의 교수몰입도에 정서적 지지, 평가적 지지 순으로 영향을 미치는 것으로 나타났다. 한편 유아교사의 직무스트레스는 교수몰입도에 상대적으로 미미한 영향력을 미쳐 이들 간의 관계를 명확히 파악하기 위한 추후 연구가 필요하다. 이러한 결과는 유아교사의 교수몰입도를 높이기 위한 기초자료가 될 수 있으며, 높은 교수몰입도는 유아교사의 삶의 질을 향상시키고 교육의 질을 증진시켜 수업전문성 향상에 기여할 수 있음을 시사한다.

DBP 스폰지와 DBP/PLGA 지지체에서의 인간 디스크세포 거동분석 비교

이선경(Seon Kyoung Lee),홍희경(Hee Kyung Hong),김수진(Su Jin Kim),김용기(Yong Ki Kim),송이슬(Yi Seul Song),하윤(Yoon Ha),이동원(Dong Won Lee),강길선(Gil Son Khang) 한국고분자학회 2010 폴리머 Vol.34 No.5

본 연구팀은 DBP를 함침시킨 물성이 서로 다른 스폰지와 PLGA 지지체를 제작한 후 세포 부착, 증식 및 형태 유지를 알아보기 위한 실험을 수행하였다. WST 분석법과 SEM 관찰을 통하여 스폰지에 비해서 PLGA 지지체에서의 세포의 증식이 활발한 것을 확인하였고, RT-PCR을 통해 디스크세포에서 특이적으로 발현하는 제2형 콜라겐과 어그리칸의 발현을 확인하였다. WST 결과, 세포 증식률은 DBP/PLGA 지지체가 DBP를 함침시킨 스폰지보다 세포 증식률이 높음을 확인하였다. 본 연구팀은 스폰지보다 PLGA 지지체가 인간디스크의 표현형 유지 및 중식에 있어서 긍정적인 영향을 미치는 것을 확인하였다. We fabricated sponge and poly(lactide-co-glycolide) (PLGA) scaffolds impregnated demi-neralized bone particle(DBP) (DBP/PLGA) and investigated proper condition to proliferation and phenotype maintenance of intervertebral disc(IVD) cells by comparison between DBP/PLGA scaffold and DBP sponge. DBP/PIGGA scaffolds were prepared by solvent casting/salt leaching. Human IVD cells were seeded in scaffolds of two types. Cell viability and proliferation according to scaffolds were analyzed by WST assay and SEM. RT-PCR was assessed to measure mRNA expression of aggrecan and type Ⅱ collagen of human IVD cells. In WST assay results, cell viability in scaffolds impregnated DBP/PLGA scaffold were higher than DBP sponge. We could observe that disc cell mRNA expressed better in DBP/PLGA scaffold than DBP sponge. We concluded that the using of DBP/PLGA in terms of scaffold fabrication for bio-disc with human IVD cells is helpful growth of disc cells maintenance of phenotypes.

아로마테라피의 연구 동향과 한의학적 활용에 대한 고찰

이선경(Seon Kyung Lee),윤미영(Mi Young Yoon),손경우(Kyung Woo Sohn),채한(Han Chae) 한의병리학회 2011 동의생리병리학회지 Vol.25 No.3

유아교사의 직무스트레스가 행복감과 조직몰입에 미치는 영향

이선경 ( Seon Kyung Lee ),이민희 ( Min Hui Lee ),김경의 ( Kyung Eui Kim ) 한국어린이문학교육학회 2014 어린이문학교육연구 Vol.15 No.4

본 연구는 유아교사의 직무스트레스가 행복감과 조직몰입에 미치는 영향을 알아보기 위한 목적으로 실시되었다. 이를 위해 전라북도 J시, I시, G시 지역의 유치원·어린이집교사 310명을 대상으로 질문지를 통해 자료를 수집하였다. 연구결과는 다음과 같다. 첫째, 유아교사의 직무스트레스, 행복감, 조직몰입의 일반적 경향에서 전반적인 직무스트레스 수준은 보통보다 낮은 수준으로 나타났고 행복감과 조직몰입은 보통보다 높게 나타났다. 둘째, 유아교사의 직무스트레스가 높을수록 행복감은 낮게 나타났다. 셋째, 유아교사의 직무스트레스가 높을수록 조직몰입에 어려움이 있는 것으로 나타났다. 이러한 결과는 유아교사의 행복감과 조직몰입을 증진시킬 수 있는 방안에 대한 기초자료가 될 것이다. The study was conducted to find out the effects of early child teacher`s job stress on happiness and organizational commitment. To that end, data was collected through questionnaires from 310 early child teachers in J city, I city, and G city, Jeollabukdo. The results of the study are as follows. First, in general, early child teacher`s job stress is shown to be lower than usual, while happiness and organizational commitment are shown to be higher than usual. Second, the higher early child teacher`s job stress is, the lower happiness is. Third, the higher early child teacher`s job stress is, the harder organizational commitment is. These results will be the basic data for the improvement of early child teacher`s happiness and organizational commitment.