Pasteurella multocida에 대(對)한 간이적혈구(簡易赤血球) 응집반응(凝集反應)과 적혈구(赤血球)의 안정화(安定化)에 관(關)한 연구(硏究)

이학철,정영건,김교준,Lee, Hak Cheul,Chung, Yung Gun,Kim, Kyo-Joon 대한수의학회 1970 大韓獸醫學會誌 Vol.10 No.1

Recently Carter(1952) reported the capsule antigens of Pasteurella multocida could be divided into four serological types A,B,C and D by means of precipitation tests. Subsequently he showed that the most sensitive for identification of these types involved the use of capsule substance adsorbed by erythrocytes in hemagglutination test. It may be somewhat difficult to conduct the hemagglutination test in small laboratory, because relatively large amounts of antisera and erythrocytes of the human O type are required for the test. A simple method for serological typing of P. multocida was the slide agglutination test employed by Little et al. (1943) and Namioka et al. (1962), but this method is still in controversy. The author tried adapting Carter's hemagglutination method to the slide method so called "micromethod technique", and studied on the stabilization of erythrocytes for use of slide hemagglutination to P. multocida although many invesigators reported the stabilization of erythrocytes. The results obtained are summarized as follows: 1. A simplified method (slide method) for capsule typing of the organism was developed by adapting Carter's hemagglutination reaction(tube method). Antibody-containing serum can be diluted serially on Boerner's microtest slide with capillary or serological pipetts with a considerable accuracy. The slide reaction can be carried out with case on the slide by adding $0.05m{\ell}$ of antigen-sensitized erythrocytes suspension diluted to one percent on $0.05m{\ell}$ of serially diluted antibody-containing sera, and the final result can be read after 60 minutes at the room temperature ($15^{\circ}C$). 2. It is difficult to determine superiority of inferiority between the slide method and the tube method on the pattern of the reaction of hemagglutination. 3. The pH range of 6.6 to 8.3 is optimal for the slide hemagglutination reaction. 4. The antigen-sensitization against erythrocytes at $37^{\circ}C$ is optimal for the slide hemagglutination. 5. Both the doses and concentration of antigen do not influence the antigen-adsorbing capacity of erythrocytes. 6. The reduction of antigen-sensitizing hours does not influence the antigen-adsorbing capacity of erythrocytes even 30 minutes. 7. The tannic acid treatment against formalinized and non-formalinized erythrocytes showed no effect on the reaction of hemagglutination. 8. The erythrocytes preserved at $4^{\circ}C$ in the ACD solution do not decrease the reactivity on the reaction of hemagglutination for 60 days, while they begin slight hemolysis 30 days after preserving. 9. The stable preparation of erythrocytes can be obtained by treating the cells at $37^{\circ}C$ for 20 hours with from 4 to 8 percent of formalin in saline or buffer. These cells can be preserved at $4^{\circ}C$ for more than 8 months experimented without hemolysis. With low concentration of formalin, the cells were not sufficiently stabilized resulting in the hemolysis after short period of preservation at $4^{\circ}C$. 10. The erythrocytes treated with 16 percent of formalin remain constantly or increase the reactivity for the reaction of hemagglutination. On the contrary, the cells treated with I to 8 percent of formalin decrease the reactivity. 11. There is no difference between nontreated fresh erythrocytes and the erythrocytes preserved in the ACD solution on the reactivity against the hemagglutination, and the erythrocytes treated with 16 percent of formalin showed the reactivity of higher level than that of the above two kinds of erythrocytes. 12. There is no difference between the saline and the isotonic buffer solution on the reaction of hemagglutination.

우리나라 축우(畜牛)의 Eperythrozoon 감염(感染)에 관(關)한 연구(硏究) I. Eperythrozoon 감염(感染)의 실태조사(實態調査)

이학철,손제영,유동열,정영건,Lee, Hak Cheul,Son, Jae Young,Yu, Tong Yeul,Chung, Yung Gun 대한수의학회 1969 大韓獸醫學會誌 Vol.9 No.2

A survey on the Eperythrozoon infection of cattle was conducted by means of examinations of Giemsa staining blood films obtained from 2,263 cattle which had been fed in the middle and south parts of Korea. On the other hand, continuous hematological examinations to five splenectomized calves were performed. The results obtained were summarized as follows: 1. Eperythrozoon wenyoni were detected in the blood films of five cattle from Kyungpook and Cheju areas. 2. The infections of Eperythrozoon wenyoni were observed in four of five splenectomized calves from Taegu and Sungwhan areas. 3. From these results it is considered that the infections of Eperythrozoon wenyoni in cattle are quite common in the middle and south parts of Korea.

도축돈에서의 Haemophilus Pleuropneumoniae 감염에 관한 연구

이학철,유병삼,Lee Hak-Cheul,Yoo Byong-Sam 한국임상수의학회 1986 한국임상수의학회지 Vol.3 No.1

In recent years much attention has been paid to swine respiratory infection caused by Haemophilus(H.) Pleuropneumoniae with rapid expansion of pork industry in Korea. The organism may cause an acute respiratory infection with high morbidity and mortality, or it may also cause chronic persistent infection, resulting in loss of body weight. The present study was performed to observe the etiologic situation of H. Pleuropneumoniae infection by evaluating bacteriological, serological examinations with the lungs and sera collected from slaughtered pigs in Daegu city, respectively. In addition, antibiotic susceptibility test was carried out against the isolated strains of H. Pleuropneumoniae. The results obtained are summarized as follows : 1. Only 10 strains of H. Pleuropneumoniae were isolated from the 120 cases of swine lungs examined, and biological and biochemical characteristics of the isolates were identical with that of standard strains(serovar 1 to 6). 2. Out of 336 swine sera examined, 108(32.1%) were shown to be positive against H. Pleuropneumoniae by latex agglutination test. 3. Antibiotic susceptibility test revealed that 10 isolates of H. Pleuropneumoniae were sensitive to 16 kinds of antibiotic such as ampicillin, benzylpenicillin, piperacillin, cephalothin, cefazolin, cefamandol, cefoperagon, lactomoxycefa, tetracycline, minocycline, chloramphenicol, gentamicin, kanamycin, amikacin, colistin and nalidixic acid, whereas cloxacillin, erythromycin, kitasamycin, lincomycin, oleand-amycin, and bacitracin were resistent.

'81 , '82 년도 연구소원 본지 게재외 논문초록 : 뉴캣슬병 면역에 대한 검토 ; 1 . 뉴캣슬병 유행지역에서의 계군면역에 대한 실태조사

이학철(Hak Cheul Lee),정유열(Yue Yerl Chung) 영남대학교 자원문제연구소 1983 資源問題硏究 Vol.2 No.-

마우스 및 랫트의 Sendai virus, mouse hepatitis Virus, Mycoplama pulmonis 감염(感染)에 대한 보체결합반응(補體結合反應)과 효소표식면역흡착측정법(酵素標識免疫吸着測定法)과의 비교(比較)

정유열,이학철,이은,유병삼,Chung, Yoo-yeul,Lee, Hak-cheul,Lee, Eun,Yoo, Byung-sam 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.4

This study was undertaken to establish reliable diagnostic-procedures for the microbiological monitoring of laboratory animals. Murine(mice and rats) antibodies against hemagglutinating virus of Japan(HVJ), mouse hepatitis virus(MHV) and Mycoplasma pulmonis(Mp) were detected sensitively and specifically in experimentally and naturally infected animals' sera by an indirect enzyme-linked immunosorbent assay(ELISA), using urease conjugated antimurine immunoglobulin. The sensitivity and specificity of the complement fixation test which has been apllied widely for serodiagnosis of HVJ, MHV and Mp infections were apparently lower than those of ELISA. From these results, the ELISA was found to be available for the serodiagnosis of HVJ, MHV and Mp infections in mice and rats.

실험용(實驗用) 마우스의 Mycoplamsa감염(感染) 실태(實態)와 분리주(分離株)의 항생제(抗生劑) 감수성(感受性)에 관한 연구(硏究)

정유열,조성룡,이학철,Chung, Yoo-yeal,Cho, Sung-yong,Lee, Hak-cheul 대한수의학회 1986 大韓獸醫學會誌 Vol.26 No.2

Isolation and identification of Mycoplasma were performed to clarify Mycoplasma infection of mice fed by conventional feeding at two ($K_1$, $K_2$) institutes in Korea. The twenty mice to be tested were randomly sampled from each of 10 breeding colonies in respective institute. Identification of the Mycoplasma strains isolated from the nasal cavity, lung and synovia of mice was made according to the morphology of colonies, biological and biochemical properties with special reference to M. pulmonis, M. arthrotodis and M. neurolyticum. In addition, growth inhibition test was performed using hyperimmune rabbit antisera to the strain PG-22 of M. pulmonis, the strain PG-6 of M, arthritidis and the strain PG-28 of M. neurolyticum and also differentiation of isolates from L-form bacteria was dont by Dieses staining and culture method with passage of the isolates on liquid media eliminated antibacterial drug. On the other hand, a total of 13 strains out of the 44 isolated M. pulmonis from mice was investigated for their susceptibility against 16 antibiotics in vitro. The antibiotic sensitivity test was made using $3{\times}10^4$ organisms/0.3ml on each plate(90mm diameter) with antibiotic mono-or tri-disk. The results obtained are summarized as follows: 1. Out of 20 mice from 10 breeding colonies in Kl institute, mycoplasma-like strains from the nasal cavity of 16 mice(80%) and from the lung of 8 mice(40%) were isolated, while out of 20 mice in K2 institute, M-like strains were isolated from the nasal cavity of 14 mice(70%) and from the lung of 6 mice(30%). However, no mycoplasma-like organisms were isolated from the synovia of the 40 mice examined. All the 44 strains isolated were identified as the organisms of M. pulmonis. 2. Out of the 16 antibiotics tested, penicillin, oleandomycin and bacitracin showed no activity against all the 13 M. pulmonis strains. On the contrary, lincomycin, clindamycin, chloramphenicol, tetracycline, minocycline, kanamycin, gentamycin and tobramycin showed high activity with three different antibiotic concentration of tridisk, but amikasin and spiramycin showed intermediate activity. Other antibiotics such as polymyxin B and colistin showed low activity, while erythromycin showed lower activity than others.

國內飼育 實驗動物에 대한 微生物學的 모니터링에 관한 硏究

鄭由烈(Yoo Yeul Chung),李學喆(Hak Cheul Lee) 한국실험동물학회 1994 Laboratory Animal Research Vol.10 No.1

國內飼育 實驗動物에 대한 微生物學的 모니터링에 관한 硏究

鄭由烈(Chung Yoo-Yeul),李學喆(Lee Hak-Cheul),兪炳三(Yoo Byung-Sam) 한국실험동물학회 1989 Laboratory Animal Research Vol.5 No.1

國內飼育 實驗動物에 관한 微生物學的 모니터링에 관한 硏究

鄭由烈(Chung Yoo-Yeul),李學喆(Lee Hak-Cheul) 한국실험동물학회 1992 Laboratory Animal Research Vol.8 No.1

Monensin이 反芻動物 第一胃內 微生物과 그 活性에 미치는 影響에 關한 硏究

李學喆,李垠 영남대학교 자원문제연구소 1983 資源問題硏究 Vol.2 No.-

The present experiments were conducted to determine the effect of feeding monensin on rumen bacterial flora and protozoal fauna, on bacterial enzyme activity involving amylase and urease, on ruminal pH and volattile fatty acids. Nine Korean native goats averaging 15.6 kg of body weight were randomly alloted to the diets supplemeted with monensin at the level of 0, 30 and 60ppm. Alters 3 weeks adaptation period the animals were fed the experimental diets. Ruminal fluids were collected and examined just before feeding, and 2, 4, 6 and 10 hrs after feeding on days 0, 7, 14, 28, and 42. 1. The total number of ruminal protozoa was markedly decreased with monensin diets from the 7th to 28th day of the experimental period, although it appeared to be recovered gradually towards the end of experiments. However, a significant difference (p<.05) among treatments could be noted in the total number of ruminal protozoal throughout the experimental period. 2. A total of 20 protozoal species were detected throughout the period: 17 species in Ophryoscolacidae, 3 species in Isotrichide. The number of those was decreased by mnonensin diets, so that the protozal composition of monensin groups was simpler than that of the control. 3. The protozoal fluctuation pattern was changed by the monensin treatment, and the fluctuation of each species consisted of the protozoal composition was varied according to not only species, but also treatments. The counts of protozoa of Holctricha order were appeared to be increased by monensin treatments while no significant differences were noted in counts of protozoa of Spirotricha-order except Eudiplodinium species shown slight increase number. 4. In the total number of ruminal bacteria, nonsignificant difference (p>.05) was observed throughout the experimental period. 5. Fourteen different kinds of the ruminal bacteria types were classified by means of morphology and Gram·stain reaction. In the total number of bacterial types, nonsignifi-cant difference (p> .05) was observed among treatments. 6. The bacterial fluctuation pattern was changed by the monensin treatment, and the fluctuation of each bacterial type consisted of the bacterial composistion was varied according to bacterial types and monensin levels. The total numbers of Gram-negative bacteria increased(15.78~16.41%) with monensin treatments, while the numbers of Gram-positive bacteria decreased (69.65~73.09%) throughout the experimental period. 7. Amylase activity in ruminal fluids of the monensin diet tend to be increased fromt he 7th to 27th or 42nd day of the experimental period : although no significant difference(p) .05) in amylase activity was noted among treatments. 8. Urease activity in ruminal fluids of the 30 ppm diet appeared to be increased from the 7th to 28th day of the experiod, the 60 ppm diet tend to be decreased from the 7th to the end of the experiment. However, no significant difference(p> .05) was observed between the 30 ppm and control group, and the 60 ppm group was significant from the other two groups. 9. In the ruminal pH, values nosignificant difference (p> .05) was found among treat-ments throughout the experimental period. 10. The total rumial VFA concentration was found not to be affected by the monensin treatments. 11. The molar portion of acetate and butyrate in rumen fluids were reduced(p <.06) by monensin diets, propionate, however, increased(p <.05) from the 7th to the end of the-experirnental period. The butyrate was significantly decreased at the 60 ppm level. Non-significant difference (p> .05) was observed in isovalerate and valerate. 12. A significant difference(p <.05) in the average ratio acetate to propionate between 60 ppm level and either control or 30 ppm was observed.