http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Lee Hak-Cheul (검색결과 98 건)
- 무료
- 기관 내 무료
- 유료
-
Wassermann反應의 變法에 關한 硏究 : 特히 Kolmer氏法에 對하여 Especially Kolmer's Method
李學喆,梁學道 慶北大學校 1962 論文集 Vol.5 No.-
The serum of the human suspected of being infected with Treponema pallidum has been tested by the Wassermann reaction, precipitation test (including flocculation test), Treponema pallidum agglutination test of Treponema pallidum immobilization test etc. The Wassermann reaction, however, has been found to be one of the most useful and reliable methods employed as laboratory aids to diagnosis. The methods of performing the test are legion and many modifications have been suggested to increase its reliability and sensitivity. Especially the cardiolipin-lecithin-cholesterol antigen investigated by Pangborn replaced effectively the crude tissue extract in most of current serodiagnostic test for syphilis. The authors undertook to disgnose a number of serum of the human suspected of being infected with syphilis in Pusan area, and the specimens were examined by Kolmer's method (serum dilution method) from the spring to the autumn of 1946. As a antigen the alcholic extract(added an alcholic solution of cholesterol) of heart-muscle from the healthy calf was employed in this examination. In performing the above procedure, the employed complement had to be treated with the cold method so as to be free of normal hemolysin in it, and varying amounts of the definite dilution of each of hemolysin, antigen and parient's serum were set up in a series of test tube, then they were titrated or examined with red blood cells and the other reagents respectively. Inasmuch as these amounts vary the volume of fluid in each tube was regulated by adding the amount of saline solution which brings the volume to 3㎖. or 4㎖. The various amounts are given in Table 1. In these respects the above method was extremely inconvenient, so that following modified method was made in our laboratory. The method is given in Table 3. 1. The amount of suspension of red blood cells was kept constant (2.5% 0.5㎖.) and the reagent to be titrated or examined in the preliminary test and complement fixation test was set up in a series of test tube with each 0.5㎖. of it diluted by two-fold series dilution(except the titration of complement), then each 0.5㎖. of saline solution was added to a series of test tube in order to bring the volume to 2.5㎖. 2. In the first place, the titration of hemolysin was tested with the complement including normal hemolysin and red blood cells, then the titration of complement was tested by using two units of the previously titrated hemolysin (included into 0.5㎖. of the definite dilution). And each two units of the titrated hemolysin and complement (included into 0.5㎖. of the definite dilution) were used in the following preliminary test (the titration of anticomplementary effect of antigen and antigenicity of antigen) and complement fixation test. 3. The complement fixation test was cardiod out with 0.5㎖. of the definite dilution of antigen determined by previous titraion and above each two units of hemolysin and complement. Repeated experiments with the above modified method have given consistently satisfactory results. The princeples of its procedure are in no way different from Kolmer's method and rationale of complement fixation in general.
-
-
Newcastle病毒 接種雛의 眠房水로부터의 病毒分離에 對하여
李學喆,趙漢喆 慶北大學校 1962 論文集 Vol.6 No.-
Methods requiring virus isolation and confirmation of the virus as a means of diagnosing Newcastle disease (ND) are time consuming. Simple and rapid tests that have been proposed take advantage of the fact that ND virus belong to the myxovirus gropp and hemoagglutinates red blood cells. Clark et al. reported the use of the aqueous humor from eyes of chickens in the incipient stages of ND as a souce of antigen for HA testing. They indicate that the virus exists in this fluid before the blood serum inhibits the HA reaction. The same authors, after further studies with aqueous humor from asymptomatic vaccinated and unvaccinated chickens, concluded that the aqueous humor from asymptomatic vaccinated and unvaccinated chickens, concluded that the aqueous humor served as a reservior for ND virus. Dardiri et al., in contrast, could not demonstrate the virus in the aqueous humor of vaccinated chickens that survived challenge. Further study is needed to determine the persistence of the virus in the aqueous humor of convalesent and immune birds. This study, therefore, was conducted to investigate the persistence of ND virus In the aqueous humor of young chickens experimentally inoculated with the virulent strain (Kyojoungwon strain) and experimentally vaccinated with single dose of the B_1 living virus vaccine and challenged. The results are surmmarized as fellows : 1. ND virus was isolated from the aqueous humor both of vaccinated and unvaccinated chickens for the first about week following exposure to the virulent strin, however in previously vaccinated chickens, isolation of the challenged virus was sparse but in susceptible chickens, readily isolated from almost all of them except the few case. 2. Both of the vaccinated and unvaccinated chickens which took a prolonged course were not isolated readily.
-
李學喆 慶北大學校 1958 論文集 Vol.2 No.-
Nakamura and his associates reported that the pathogenesis of rabbit-passaged Strain Ⅲ of the Nakamura lapinized rinderpest virus (hereinafter referred to as the "Strain Ⅲ") is fixed in rabbit and cattle. However, we found that a serial passage of this "Strain Ⅲ" and its p thogenesis become more virulent and almost all the rabbits that were infected with this "Strain Ⅲ" died in 1947. If we assume that the increase in its virulence is due to the variation of its pathogenesis by the more serial passage of this "Strain Ⅲ", it is an importanr problem in the preventaion of epidemics of Rinderpest virus in cattle. An attempt was made in our laboratory to investigate the variation of the "Strain Ⅲ" and the following conclusions were made. 1) The increase of death rate in rabbit is not due to a mixinfection of other pathogenic organisms nor symbiosis with other organisms but it has been proved that it is due to the variation of its pathogenesis. 2) The pathologic change and the necrotic process of the lymph follicular cells in infected rabbits seem to be different in this study from the one reported previously by others. Particularly the cause of sever hemorrhagic change in the mucosa of gastrointestinal tract is not clear and more study will be needed to clearly its pathogenesis. 3) The minial infectious dose of this "Strain Ⅲ" in rabbits is higher in this study than in previous studies. 4) The neutralizaing potency of the convalescent serum of infected rabbit is more lower in this study than in previous works. 5) The following results were obtained by an inoculation of this "Strain Ⅲ" to Korean calves. a) The minimal infectious dose for calves is much higher in this study than in previous studies. b) There were died case and recovered case in claves inoculate with this "Strain Ⅲ". This is similar in this study to the previous works. c) Some difference was noted in appearance of critical symptoms in this study. d) The entire course and incubation period of infected calves were shorted considerably in this study than in previous works. 6) The results of simultaneous inoculation with this "Strain Ⅲ" plus antirinder pest hyperimmune serum to Korean calves also indicated that the pathogenesis of this "Strain Ⅲ" becaome more virulent than in previous studies. 7) The successive passage of theis "Strain Ⅲ" through Korean calves showed the following results. a) In calves the pathogenesis become more virulent but at no time it acquired the virulence of Rinderpest virus. b) The death rate of infected calves is higher in this study than in previous studies. c) When the blood of infected calves were inoculated into rabbits, fever and necrosis of the lymph follicular cells were seen and this characteristics of this "Strain Ⅲ" was maintained unitl the last passage of this study. d) Minimal infectious dose of blood from infected calves was markedly low ans this is due to the difficulty in reproducation of this "Strain Ⅲ" in calves. 8) The simultaneous inoculation with this "Strain Ⅲ" plus antirinderpest hype rimmune serum which is used in Korea is safe for cattles but it is dangerous for calves. For this reason increased dose of immune serum must be used for calves.
-
大邱市內에 있어서의 山羊 및 緬羊의 結核症에 對한 調査
孫濟英,金容珌,李學喆 慶北大學校 1962 論文集 Vol.5 No.-
Tuberculosis is rare in sheep and goats. In sheep most of the infection that have been typed in the United States were found to be of the avian-type, but in orther countries most have proved to be of the bovine-type. The data on tuberculosis in goats are very meager, so this fact gives an impression on us that goats are relatively resistant to infection. Infections, however, with human-type, bovine-type and aviane-type have been rarely reported. The obtained specimens (lung, liver, spleen) from a died goat at Taegu Agriculture and Forestry High school in March 1960 were examined bacteriologically and pathologically to diagnose the cause of its death. Moreover, the 10 sheep and 13 goats which had been together in the same pen with the died goat and the 11 sheep and 202 goats which had been fed in the surrounding area of Taegu city were tested on the tuberculin reaction with the intradermal method. The tuberculin was mamalian type (O.T.). The results thus obtained would be outlined as follows: 1. The death of the goat at above school was caused with a generalized tuberculosis infected through respiratory route by the bovine-type of tubercle bacilli. 2. The result of tuberculin test have proved that among 10 sheep and 13 goats which were fed together with the died goat, the positive reaction was found in 2 sheep (20%) and 2 goats (about 15.4%) and the doubtful reaction was found in 1 sheep (10%) and 2 goats (about 15.4%), but among 11 sheep and 202 goats which were fed in the surrounding area of Taegu city, the positive and doubtful reaction were found in 2 goats (about 1%) and 3 goats (about 1.5%) respectively, and all of the sheep reacted in the negative.
-
Lee, Hwi-Cheul,Im, Gi-Sun,Chung, Hak-Jae,Lee, Poong-Yeon,Park, Jin-Ki,Chang, Won-Kyong,Yang, Boh-Suk,Yamanouchi, Keitaro,Nishihara, Masugi The Korean Society of Animal Reproduction 2006 Reproductive & developmental biology Vol.30 No.4
Our previous research has identified granulin (grn) and p130 genes as sex steroid-inducible genes in the rat hypothalamus, which might be involved in sexual differentiation of the brain. Phthalate esters that are used as plasticizers and also found at low levels in foods such as dairy products are often mentioned as suspected endocrine disrupters. The purpose of the present study is to elucidate whether perinatal exposure to di-n-butyl phthalate (DBP), diisononyl phthalate (DINP) and di-2-ethylhexyl adipate (DEHA) affects hypothalamic sex steroid-inducible genes. The present study assessed the effects of perinatal exposure to DBP, DINP and DEHA on sex steroid hormones levels and hypothalamic gm and p130 mRNA expressions at postnatal day (PND) 3 and 7. Pregnant rats were fed a soy-free diet containing 20, 200, 2,000 and 10,000 ppm of DBP, 40, 400, 4,000 and 20,000 ppm of DINP, or 480, 2,400 and 12,000 ppm of DEHA from gestational day (GD) 15 to GD 3 or 7. At PND 3 and 7, perinatal exposure to these chemicals did not substantially affect serum concentrations of testosterone and estradiol. At PND 3, the expression of grn mRNA levels in males was decreased by DEHA, and that of p130 was decreased by DBP, DINP and DEHA, though the effects were not dose-dependent. At PND 7, the expression of gm gene in female pups was increased by higher doses of DBP and all the doses, except for 4,000 ppm, of DINP, while that in male pups decreased by 480 and 12,000 ppm of DEHA. Hypothalamic expression of p130 mRNA in males was increased by lower doses of DBP and all the doses of DINP, whereas that of females was decreased by 480 and 2,400 ppm of DEHA. These results suggest that these chemicals may affect the expression of gm and p130 genes by directly acting on the hypothalamus, thus leading to inappropriate expression of these genes.
-
-
Study on the Reproductive Function in Transgenic Pig Harboring Human Erythropoietin (hEPO) Gene
Lee, Hyun-Gi,Lee, Hwi-Cheul,Chung, Hak-Jae,Hwang, In-Sul,Choi, Myoung-Seob,Byun, Sung-June,Lee, Seung-Hoon,Kim, Min-Ji,Woo, Jae-Seok,Chang, Won-Kyong,Lee, Poong-Yeon,Lee, Hoon-Taek,Park, Jin-Ki The Korean Society of Animal Reproduction 2008 Reproductive & developmental biology Vol.32 No.2
Our previous study showed that transgenic (TG) pigs harboring human EPO (hEPO) gene have been shown to have reproductive disorders, including low pregnancy rates, irregular estrus cycle and low little size. To investigate these reasons, we assessed estrus behavior (standing response) and plasma $17{\beta}$-estradiol ($E_2$) level, which partly reflect reproductive function, during the estrus cycles after synchronization and superovulation by hormone treatments. Then, we analysed blood composition and expression of hEPO gene in TG pigs. Pigs were injected with PG600. After 10 days, pigs were fed with Regumate porcine for 6 days. Blood samples were collected from jugular vein. Analysis of blood composition and $E_2$ level were measured by Hemavet 950 and $E_2$ ELISA kit, respectively. And, the expression of hEPO gene in reproductive organs was quantitated by real-time RT-PCR. The percentage of estrus behavior in TG was significantly decreased. Hematocrit (HCT), hemoglobin (Hb) concentration and red blood cell (RBC) number were significantly higher in TG than wild type (WT). On the other hand, high expression of hEPO gene in TG was observed in the mammary gland as well as in the uterus. Moreover, plasma $E_2$ level was significantly higher in TG than WT. These results suggest that nonspecific expression of hEPO gene in the other organs of TG may affect blood composition and plasma $E_2$ level, thereby causing reproductive disorders.
-
Lee, Hak-Cheul,Son, Jae-Young 慶北大學校 1967 論文集 Vol.11 No.-
Newcastle病(ND)豫防液로서 Blacksburg Straiu(B_1) Virus를 우리 나라에導入한 以來 이 Virus를 應用한 幼雛免疫에 있어 몇가지의 報告가 있으나 모두 母鷄으로부터 이어받은 幼雛가 지니고 있는 先天的 免疫性을 考慮치 않은 것이었다. 著者는 앞서 그에 對한 基礎的 問題의 하나를 밝히기 위하여 母鷄로부터 初生雛에 移行된 ND, 血球凝集抑制(HI)抗體의 消長과 母鷄, 이에 由來한 鷄卵의 卵黃 및 初生雛間의 三者의 力價에 關해서도 比較檢討한 바가 있다. 이번의 硏究 目的은 上記知見을 基礎로하여 常規免疫鷄, 高度免疫鷄由來幼雛에 對하여 接種經路를 달리해서 B_1 Virus를 應用한 免疫成績에 關하여 比較觀察하였다. 이번 硏究에서 얻은 結論을 要約하면 다음과같다. 先天的免疫性을 지니고있는 幼雛들은 B_1 Virus 筋肉內接種에 對하여 妨害現象을 나타내어 免疫이 全혀 成立되지 않았으나 同量病毒을 鼻孔點適注入한 것에 있어서는 感染이 發展되었으며 攻擊毒의 筋肉內后毒接種에 對하여 어느 程度 低抗하였다. Chicks with congenital passive immunity appear refractory to intramuscular vaccination with Blacksburg strain(B_1) virus; however, the same chicks vaccinated with an identical dose of the same virus by nasal instillation seem to develop infection and subsequent immunity to intramuscular challenge exposure.
-
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
