쥐의 자궁에서 아릴썰파타아제 A의 분리 및 그 성질에 관한 연구

황인환,양철학 ( In Hwan Hwang,Chul Hak Yang ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.1

Arylsulfatase A (aryl sulfate sulfohydrolase; E.C. 3. 1. 6. 1) from rat uterus was highly purified by ammonium sulfate fractionation, DEAE-cellulose chromatography, Sephadex G-200 gel filtration. Conconavalin A-Sepharose affinity chramatography, and second gel filtration on Sephadex G-200. Arylsulfatase A was separated from arylsulfatases B and Bm by DEAE-cellulose column where arylsulfatase A was eluted at 0. 2 M of NaCl linear gradient. The enzyme was purified 207-fold with 8 % recovery, but disc polyacrylamide-gel electrophoresis showed that the purified preparation was not homogeneous. Arylsulfatase A showed the anomalous time-activity relationship. The K_m and V_(mas) were 6.8 mM and 15.6 nmol/min, respectively. Arylsulfatase A had two pH optima at pH 5. 2 and 5.6 for 10 min incubation time, and these optima shifted to pH 5.0 and 5.6 with incubation time of 60 min. SO₄^(2-), PO₄₃^(2-), and PO₄^(2-) were strong inhibitors with Ki values of 4.3×10^(-3)M, 6.6×10^(-5)M, and 3.0×10^(-6)M, respectively. Pyrophosphate and Cu^(2+) ion inhibited arylsulfatase A but Mn^(2+), Ca^(2+), Co^(2+), and ascorbic acid activated the enzyme activity. Siver and Cl- ion did not affect the enzyme activity. Enzyme activity was increased to 60 ℃ for 10 min and thermally stable at 60 ℃ for 70 min with 82 % of activity. By Sephadex G-200 gel filtration the molecular weight of arylsulfatase A was 120,000 at both pH 5.0 and 7.2.

'AKR2 유전자' 통해 엽록체 생성 기원 밝혔다

황인환,Hwang, In-Hwan 한국과학기술단체총연합회 2008 과학과 기술 Vol.41 No.2

사무용 건물의 에너지 절약설계수법

황인환,Hwang, In-Hwan 한국설비기술협회 1992 설비 : 공조ㆍ냉동ㆍ위생 Vol.9 No.7

Arylsulphatases B and Bm from Rat Uterus: Their Purifications and Characterizations

황인환,양철학,Hwang, In-Hwan,Yang, Chul-Hak 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.2

아릴썰파타아제 B 및 Bm(아렬썰페이트 썰포하이드롤라아제 ; E.C.3.1.6.1)이 쥐의 자궁으로부터 분리되고 그 특성이 연구되었다. 아릴썰파타아제 B는 286배로 정제되고 그 수율은 23%이었으며 Bm은 112배 정제되고 그 수율은 5.4%이었다. 통판 전기영동에 의해 마지막으로 얻어진 아릴썰파타아제 B 및 Bm은 하나의 단백질 띠로 나타났다. 이 효소들은 파라-나이트로카테콜썰페이트를 기질로 사용했을 때 정상적인 시간-활동도의 관계를 나타냈다. $K_m$과 $V_{max}$ 값은 아릴썰파타아제 B가 $2.0{\times}10^{-3}M$ 및 20.8 nmol/min 이었고 아릴썰파타아제 Bm은 $3.9{\times}10^{-3}M$ 및 67 nmol/min 이었다. 쥐의 자궁은 상당한 양의 음성형의 아릴썰파타아제 B(즉 아릴썰파타아제 Bm)를 함유하고 있었다. 아릴썰파타아제 Bm은 물리화학적 성질 가운데서 pH 적정점, 열에 대한 안정도, 썰페이트, 썰파이트 및 포스페이트에 대한 방해작용이나 시간-활동도의 관계에 있어 아릴썰파타아제 B와 거의 비슷한 결과를 나타냈다. Arylsulphatases B and Bm (arylsulphate sulphohydrolase; E.C.3.1.6.1) from rat uterus were purified and characterized. Arylsulphatases B and Bm were purified 286-fold and 1I2-fold with an overall recovery 23% and 5.4%, respectively. On disc gel electrophoresis, both final preparations of arylsulphatases B and Bm showed one protein band. These enzymes showed normal time-activity relationship with p-nitrocatechol sulphate as substrate. The $K_m$ and $V_{max}$ values were $2.0{\times}10^{-3}\;M$ and 20.8 nmol/min for arylsulphatase Band $3.9{\times}10^{-3}\;M$ and 67 nmol/min for arylsulphatase Bm, respectively. Rat uterus contained significant quantities (16~20% of total arylsulphatase) of an anionic form of arylsulphatase B (arylsulphatase Bm). The physico-chemical properties of arylsulphatase Bm were very similar to those of arylsulphatase B with respect to pH optimum, heat stability, the types of inhibition by sulphate, sulphite and phosphate ions, molecular weight, temperature optimum, and time-activity relationship.

쥐의 자궁에서 아릴썰파타아제 B 및 Bm의 분리 및 그 효소의 특성에 관한 연구

황인환,양철학 ( In Hwan Hwang,Chul Hak Yang ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.2

Arylsulphatases B and Bm (arylsulphate sulphohydrolase; E.C. 3.1.6.1) from rat uterus were purified and characterized. Arylsulphatases B and Bm were purified 286-fold and 112-fold with an overall recovery 23% and 5.4% respectively. On disc gel electrophoresis, both final preparations of arylsulphatases B and Bm showed one protein band. These enzymes showed normal time-activity relationship with p-nitrocatechol sulphate as substrate. The K_m and V_(max) values were 2.0×10^(-3) M and 20.8 nmol/min for arylsulphatase B and 3.9×10^(-3) M and 67 nmol/min for arylsulphatase Bm, respectively. Rat uterus contained significant quantities (16∼20% of total arylsulphatase) of an anionic form of arylsulphatase B (arylsulphatase Bm). The physico-chemical properties of arylsulphatase Bm were very similar to those of arylsulphatase B with respect to pH optimum, heat stability, the types of inhibition by sulphate, sulphite and phosphate ions, molecular weight, temperature optimum, and time-activity relationship.

족관절 삼과 골절과 동반된 장 비골건 탈구(1예 보고)

황인환,김광열,김형천,임문섭,이지훈,조성준,Hwang, In-Hwan,Kim, Kwang-Yul,Kim, Hyung-Chun,Lim, Moon-Sup,Lee, Ji-Hoon,Cho, Sung-Jun 대한족부족관절학회 2009 대한족부족관절학회지 Vol.13 No.2

Traumatic dislocation of the peroneus longus tendon is an often unrecognized injury which has been reported to occur most commonly during sports activity. Most acute cases with early detection can be treated by simple repair of the tendon or retinaculum using one of several procedures available. Accurate treatment through a comprehensive evaluation of the ankle injury prevents suboptimal and sometimes unrecorrectable outcomes. The authors experienced concomitantly the peroneus longus tendon dislocation associated with a trimalleolar fracture of the ankle. We report this case a brief review of the literature.

조현병 환자의 입원 치료시 약물처방 경향의 변화 : 일 대학병원에서 1996~2000년과 2006~2010년의 차이 비교

황인환,김대호,오대영,Hwang, In-Hwan,Kim, Daeho,Oh, Dae-Young 대한생물정신의학회 2014 생물정신의학 Vol.21 No.2

Objectives Previous literature on the prescription change among patients with schizophrenia mainly focused on antipsychotics. This study investigated chronological change in the patterns of discharge medication among inpatients with schizophrenia at a psychiatric inpatient unit of a university-affiliated hospital. Methods All admission records at a psychiatric unit of Hanyang University Guri Hospital with discharge diagnosis of schizophrenia during two different five-year time frames (1996-2000 and 2006-2010) were reviewed including the demographic and clinical data and discharge medications. The data were gathered from a total of 207 patients (95 in 1990s and 112 in 2000s). Results The frequency in use of atypical antipsychotics (p < 0.01), antidepressants (p < 0.05), beta-blockers (p < 0.01), and benzodiazepine (p < 0.01) was significantly higher in 2000s. Anticholinergic drugs were less likely used in 2000s (p < 0.01). We did not find significant differences in the equivalent dose of antipsychotic drugs, the use of mood stabilizers and cholinergic drugs between two time frames. Conclusions Increased proportion of atypical antipsychotics and decreased use of anti-parkinsonian drugs are in line with literature. Our results show that more diverse classes of psychotic medications are used for schizophrenia in recent years. It is likely that psychiatrists are becoming more conscious of negative symptoms, anxiety, and depression in the pharmacotherapy of schizophrenia as well as positive symptoms of the illness.

Purification and Characterization of Arylsulfatase A from Rat Uterus

황인환,양철학,Hwang, In-Hwan,Yang, Chul-Hak 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.1

$(NH_4)_2\;SO_4$, 분획분리, DEAE-셀룰로즈 크로마토그라피, Sephadex G-200젤 여과법, Con A-Sepharose 흡착크로마토그라피 및 Sephadex G-200젤 여과법등을 이용하여 쥐의 자궁에서 아럴썰파타아제 A를 거의 순수한 상태로 정제하였다. 아랄쎌파타아제 A는 DEAE-셀룰로즈 column에 의하여 아릴썰파타아제 B와 Bm으로부터 분리되어졌고, DEAE-셀룰로즈 column으로 부터 0.2M NaCl 선형 농도증가에 의해서 흘러나왔다. 이 효소는 마지막 단계의 회수율이 8%였으며, 207배 정제되어졌으나 Disc-폴리아크릴아마이드 젤 영동법에 의하여 단일 단백질로 정제되지는 않았다. 아릴쎌파다아제 A는 비정상적인 시간에 따른 활동도 곡선을 나다냈다. 아릴썰파타아제 A는 $37^{\circ}C$에서 10 분동안의 반응에서는 pH 적정점은 pH 5.2와 pH 5.6이었는데, 60분 동안의 반응에서는 pH 5.0과 pH 5.6으로 바뀌어졌다. ${SO_4}^{2-}$, ${SO_3}^{2-}$ 및 ${PO_4}^{2-}$는 강한 방해제로서 작용하며 그들의 Ki값은 각각 $4.3{\times}10^{-3}M$, $6.6{\times}10^{-5}M$ 및 $3.0{\times}10^{-5}M$이었다. $P_2{O_7}^{2-}$와 $Cu^{2+}$ 이온은 아릴쎌파다아제 A 활성도를 방해하였으나 $Mn^{2+}$, $Co^{2+}$, $Ca^{2+}$와 ascorbic acid는 활성도를 오히려 증가시켰다. 반면에 $Ag^+$와 $Cl^-$이온은 효소의 활동에 전혀 영향을 미치지 않았다. 효소의 활성도는 $60^{\circ}C$까지 온도의 증가에 따라 계속 증가되 었으며 70분 동안 $60^{\circ}C$에서 82%의 활동도를 유지하였다. Sephadex G-200젤 여과법에 의하여 아랄쎌파타아제 A의 분자량은 pH 5.0 과 pH 7.2에서 모두 120,000 daltons으로 나타났다. Arylsulfatase A (aryl sulfate sulfohydrolase; E.C. 3.1.6.1) from rat uterus was highly purified by ammonium sulfate fractionation, DEAE-cellulose chromatography, Sephadex G-200 gel filtration. Conconavalin A-Sepharose affinity chramatography, and second gel filtration on Sephadex G-200. Arylsulfatase A was separated from arylsulfatases B and Bm by DEAE-cellulose column where arylsulfatase A was eluted at 0.2 M of NaCl linear gradient. The enzyme was purified 207-fold with 8% recovery, but disc polyacrylamide-gel electrophoresis showed that the purified preparation was not homogeneous. Arylsulfatase A showed the anomalous time-activity relationship. The $K_m$ and $V_{max}$ were 6.8 mM and 15.6 nmol/min, respectively. Arylsulfatase A had two pH optima at pH 5.2 and 5.6 for 10 min incubation time, and these optima shifted to pH 5.0 and 5.6 with incubation time of 60 min. ${SO_4}^{2-}$, ${SO_3}^{2-}$, and ${PO_4}^{2-}$ were strong inhibitors with Ki values of $4.3{\times}10^{-3}M$, $6.6{\times}10^{-5}M$, and $3.0{\times}10^{-5}M$, respectively. Pyrophosphate and $Cu^{2+}$ ion inhibited arylsulfatase A but $Mn^{2+}$, $Co^{2+}$, $Ca^{2+}$, and ascorbic acid activated the enzyme activity. Siver and $Cl^-$ ion did not affect the enzyme activity. Enzyme activity was increased to $60^{\circ}C$ for 10 min and thermally stable at $60^{\circ}C$ for 70 min with 82% of activity. By Sephadex G-200 gel filtration the molecular weight of arylsulfatase A was 120,000 at both pH 5.0 and 7.2.

이동 트랜잭션의 중복 데이터에 대한 낙관적 일치성 제어기법

황인환(In-Hwan Hwang),이종현(Jong-Hyun Lee) 한국정보과학회 1997 한국정보과학회 학술발표논문집 Vol.1997 No.4

생인손을 동반한 경피증 환자의 말단골용해

황인환(In Hwan Hwang),임문섭(Moon Sup Lim),김광열(Kwang Yul Kim),김형천(Hyung Chun Kim),문상호(Sang Ho Moon),이지훈(Ji Hoon Lee),조성준(Sung Joon Cho) 대한정형외과학회 2010 대한정형외과학회지 Vol.45 No.1

경피증(scleroderma)은 피부 및 전신의 결체 조직에 교원질이 과도하게 침착하고 미세혈관의 병변으로 가끔씩 말단골용해(acroosteolysis)을 동반하기도 한다. 말단골용해는 수족지 말단의 기저부는 유지된 채로 골파괴 또는 골용해가 발생되는 현상으로 대부분의 말단골용해는 경피증을 포함하는 결체조직 질환에서 혈류순환 장애로 인해 이차적으로 발생되는 것으로 알려져 있으나, 연부 조직의 궤양이나 부분적인 감염 및 골수염에 의해서도 발생 가능하다고 한다. 경피증을 가진 환자들은 결체 조직의 이상으로 미세혈관의 폐쇄, 피하조직의 소실, 건조하고 비후된 피부 및 면역체계의 이상 등으로 사지 밀단부에 생인손(felon) 및 피부 궤양과 같은 감염이 지속적으로 나타나기 쉽다. 저자들은 생인손을 주소로 내원하여 경피증으로 진단된 환자에게서 말단골용해 1예를 경험하였기에 이를 보고하는 바이다. The Scleroderma is a chronic inflammatory disease of the connective tissue with involvement of the skin and other organs. It can be a manifestation of various disorders and occasionally acroosteolysis in the phalanges. Acroosteolysis is characterized by bone resorption or destruction in the phalanges, while the base is preserved. The pathogenesis of acroosteolysis in patients with scleroderma is a blood-flow disorder that is mainly associated with an abnormal accumulation of collagen in all tissues, microangiopathy and infections in the phalanges. The phalanges in patients with scleroderma are prone to continuous infections as a felon or skin ulcers due to atrophy of the subcutaneous tissue, dry and sclerotic skin, or a disorder of the immune system. We experienced a patient who had acroosteolysis with scleroderma of the phalanges, and this was associated with a felon. We report on this case along with a brief review of the literature.