시맨틱 웹 서비스를 위한 Topic Maps 기반의 온톨로지 언어

황윤영(Yun-Young Hwang),유정연(Jeong-Youn Yu),유소연(So-Youn Yu),이규철(Kyu-Chul Lee) 한국전자거래학회 2003 한국전자거래학회 학술대회 발표집 Vol.2003 No.-

토픽 맵 기반의 지능적 웹서비스 발견 시스템 설계 및 구현

황윤영(Yun-Young Hwang),유정연(Jeong-Youn Yu),유소연(So-Yeon Yo),이규철(Kyu-Chul Lee) 한국전자거래학회 2004 한국전자거래학회지 Vol.9 No.4

현재, 많은 연구가 진행되고 있는 시맨틱 웹 서비스 발견 기술은 DAML-S와 MIT의 Process Handbook 프로젝트의 온톨로지를 기반하고 있다. 하지만, 이러한 발견 기술들은 현재 상황에서 지능적으로 웹 서비스를 발견하기 위해서는 여러 가지 제약 상황을 가지고 있다. 따라서 본 논문에서는 지능적 웹 서비스 발견을 위해 시맨틱 웹 서비스 발견 기술에 이용되는 기존의 온톨로지를 분석하였으며, 기존의 온톨로지가 가지는 장점을 수용하면서 단점을 보완하는 시맨틱 웹 서비스 표현 모델과 지능적 웹서비스 발견이 가능하도록 질의 언어를 제안하였다. Currently, developed technologies for semantic web services discovery are based on ontologies. These ontologies are DAML-S(DARPA Agent Markup Language for Services) and Process Handbook Project of MIT. These technologies have some problems for intelligent web services discovery. So, in this paper we analyzed those ontologies and proposed TM-S, Topic Maps for Services. TM-S is the presentation model for semantic web services. And TM-S includes benefits and complements weaknesses of those ontologies. And we proposed TMS-QL, TM-S Query Language. TMS-QL is query language for intelligent web services discovery. At last, we designed and implemented intelligent web service discovery system that deals TM-S ontology and TMS-QL.

지능적 웹 서비스 발견 시스템에서의 질의 처리

황윤영(Yun-Young Hwang),유정연(Jeong-Youn Yu),유소연(So-Yeon You),이규철(Kyu-Chul Lee) 한국정보과학회 2004 한국정보과학회 학술발표논문집 Vol.2004 No.5

부인암 환자의 생물학적 표지자로서 Lysophosphatidic Acid (LPA)의 효용성

김영재 ( Young Jae Kim ),신동열 ( Dong Yul Shin ),김승룡 ( Seung Ryong Kim ),김경태 ( Kyung Tai Kim ),황윤영 ( Youn Yeung Hwang ),윤혜란 ( Hye Ran Yoon ),조삼현 ( Sam Hyun Cho ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.11

난소암 세포주와 cisplatin 내성주에서의 MDR 관련 유전자의 발현에 관한 연구

문영진(Young Jin Moon),황윤영(Youn Yeung Hwang),이재규(Jai Kyu Lee),나영정(Young Jeong Na),이영미(Young Mi Lee),장성렬(Sung Yeoul Chang),조삼현(Sam Hyun Cho),김경태(Kyung Tai Kim) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.9

N/A Objective : Expressions of P-glycoprotein, the multidrug resistance-associated protein (MRP) and the lung resistance protein (LRP) with the MDR phenotype widely divergent in human cancer cell lines. This study focused on the altered gene expression related drug transport. Methods : To examine correlations between MDR-associated genes and PKC isozyme with cisplatin resistance on the level of the mRNA expression, we analyzed MDR-associated gene (LRP, MDR1/P-gp and MRP) expression and PKC isozyme, topoisomerase II alpha and beta in cisplatin-sensitive ovarian cancer cell line A2780 and cisplatin - resistant cell line A2780cp using cDNA-PCR approach. Results : LRP mRNA levels were significantly increased in A2780cp compared to the drug sensitive variant. In contrast, MRP mRNA levels were not significantly correlated with drug sensitivity. A modest increase in PKCη and MDR1/P-gp mRNA expression activity was also observed in ovarian cancer A2780cp cell lines that were resistant to CDDP. The level of topoisomerase II alpha and beta were not affected. Conclusion : These results showed that MDR1/P-gp expression may be an important determinant of the MDR phenotype in cell lines intrinsically resistant to cancer chemotherapeutic agents and a multifactorial emergence of MDR phenotype of tumor with a possible involvement of the PKC isozymes may be associated with CDDP resistant ovarian cancer cell line.

제왕절개술 후 발생한 T3 Thyrotoxicosis 1 예

성영모(Young Mo Sung),윤상순(Sang Soon Yoon),구미경(Mi Kyung Koo),황정혜(Jung Hye Hwang),박문일(Moon Il Park),정성노(Sung Ro Chung),황윤영(Youn Yeung Hwang),문형(Hyung Moon),이재웅(Jae Ung Lee),안유헌(You Hern Ahn) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.10

Thyroid storm (thyrotoxicosis) is rarely encountered during pregnancy or the puerperium, even in untreated women and characterized by hypertension, hyperthermia, and multiple systems involvement. Much more common is heart failure, apparently caused by the long-term myocardial effects of thyroid hormone and intensified by other pregnancy complications that include severe preeclampsia, infection, anemia, or combinations of these. Prompt diagnosis and treatment of thyroid storm are essential in reducing morbidity and mortality of a patient from this disorder. We experienced a case of triiodothyroxine induced thyroid storm (T3 thyrotoxicosis) developed after Cesarean section. This case highlights both physicians should be aware of the symptoms and risk factors and reversing of heart failure successfully by reducing cardiac workload is important through immediate medical treatment-adequate hydration, thermoregulation, and control of hypertension, infection and anemia et al.

선행 제왕절개술 후의 질식 분만 - 10 년간의 연구 -

나영정(Young Jeong Na),구미경(Mi Kyung Koo),박문일(Moon Il Park),김경태(Kyung Tai Kim),정성로(Sung Ro Chung),황윤영(Youn Yeung Hwang),문형(Hyung Moon),이유경(Yoo Kyoug Lee) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.3

N/A Objective: Recently, enormous amount of the studies about trial of labor after cesarean section has been reported to decrease the rate of cesarean section. This study focused on analyzing multiple variables and results to acknowledge the safety and complications of VBAC (vaginal birth after cesarean section). Methods & materials: This study was performed based on 97 pregnant women who were tried vaginal delivery after cesarean section at Hanyang University Hospital from January 1990 to December 1999.The age of mother, gestational age, estimated fetal body weight by ultrasonography, neonatal body weight, cervical dilatation, cervical effacement, Bishop score, the number of previous vaginal delivery, complications of mother, and complications of fetus were analyzed between successful group (82 patients)and failure group (15patients)with trial of labor. Student T-test and Chi-square test were used for statistical analysis. Results: In the 97 cases with trial of labor after cesarean, the success rate was 84.5%and failure rate was 15.5%. The estimated fetal body weight by ultrasonography, cervical dilatation and effacement, Bishop score, Apgar score had notable differences between successful group and failure group, while the other factors had not. There was no maternal death or uterine rupture in the cases of trial of labor. Conclusion: Trial of labor after cesarean section is relatively safe method of delivery if it is performed under strict indication. It could be suggested that VBAC has relatively little complication in both mother and fetus. The studies with large populations and performed by multi-centers will be needed for the evaluating safety of VBAC and developing safe protocols to decrease the risk of complications.

부인암 세포주에서 항암제 투여에 따른 신생혈관 형성 관련 유전자 전령 리보핵산 및 단백질 표현 양상에 관한 연구

김영재 ( Young Jae Kim ),공미숙 ( Mi Sook Kong ),이영미 ( Young Me Lee ),장성렬 ( Sung Yeoul Chang ),김승룡 ( Seung Ryong Kim ),김경태 ( Kyung Tai Kim ),황윤영 ( Youn Yeung Hwang ),조삼현 ( Sam Hyun Cho ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.3

연구목적: 본 연구자는 확립된 세포주에서 세포독성제 및 면역 조정제에 의한 항신혈관 형성효과를 확인하고자 하였다. 유방암 세포주 MCF-7, 자궁 경부 편평 상피암 세포주 SiHa, 난소암 세포주 A2780, 그리고 cisplatin 내성 난소암 세포주 A2780-CDDP에서 cisplatin, paclitaxe l(taxol), 및 thalidomide로 처리한 후에 vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), 그리고 thrombospondin-1,2 (TSP-1,2)의 전령 리보 핵산 및 단백질의 표현 양상의 변화를 규명하고자하였다. 연구대상 및 방법: 유방암 세포주 MCF-7, 자궁 경부 편평 상피암 세포주 SiHa, 난소암 세포주 A2780, 그리고 cisplatin 내성 난소암 세포주 A2780-CDDP에서 cisplatin, paclitaxel (taxol), 및 thalidomide로 각각 처리한 후에 vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), 그리고 thrombospondin-1,2 (TSP-1,2)의 전령 리보 핵산 및 단백질의 표현 양상의 변화를 규명하고자 RT-PCR, 단백 추출 및 Western blot assay를 사용하였다. 결과: 난소암 세포주 A2780과 동일 세포주에서 분리한 cisplatin 내성 주 A2780-CDDP, 유방암 세포주 MCF-7 및 자궁경부 편평 상피암 세포주인 SiHa에서 VEGF gene은 212 bp, bFGF는 238 bp, 그리고 TSP-1은 492 bp로 합성되었으나 TSP-2 는 합성되지 않았으며 VEGF 및 bFGF 단백질은 각각 21 KDa와 17 KDa로 합성되었으나 TSP-1 단백질은 합성되지 않았다. 각 세포주의 VEGF gene의 발현 정도는 cisplatin, taxol 및 thalidomide에 별 큰 영향을 받지 않았다. bFGF gene의 발현 정도는 taxol처리 후 유방암 세포주에서 만 감소하였으며 TSP-1 gene의 발현도는 taxol 처리 후 유방암 세포주에서 자궁경부암 세포주 보다 큰 폭의 감소가 있었고 난소암 세포주에서 thalidomide처리 후 뚜렷한 감소를 보였다. Cisplatin, taxol, 그리고 thalidomide에 VEGF 및 bFGF 단백질 발현도는 아무런 영향을 받지 않았다. 결론: 난소암 세포주 A2780, cisplatin 내성 난소암 세포주 A2780-CDDP, 유방암 세포주 MCF-7, 그리고 자궁경부 편평 상피암 세포주 SiHa에서 VEGF, bFGF 그리고 TSP-1 gene들 및 단백질의 발현 양상은 cisplatin 처리에 의해 변화되지는 않는 것으로 확인되어 난소암, 유방암 그리고 자궁경부암에서 cisplatin의 신혈관 형성 억제 효과 판정에는 VEGF, bFGF 및 TSP-1 등의 직접적인 단백 발현 양상만을 표적으로 삼기 보다는 기저층 파괴를 담당하는 신호 전달체등의 발현 변화를 동시에 분석하여야 할 것으로 사료되며, 통계적으로 유의하지는 않았지만 taxol 처리에 따른 신혈관 형성 단백 유전자들의 발현 양상에 있어 bFGF가 유방암 세포주에서 약간 감소되었고, TSP-1은 유방암 세포주에서 자궁경부암 세포주보다 약간 더 많은 감소를 보여 bFGF와 TSP-1은 유방암 및 자궁경부암에서 taxol의 신혈관 형성 저해 연구의 평가 기준으로 재연구될 필요가 있을 것으로 생각된다. 역시 통계적으로 유의한 정도는 아니었으나 Thalidomide 처리에 따른 전기 단백질들의 표현 양상에는 난소암 세포주 A2780에서 TSP-1 단백 발현의 감소가 다른 세포주보다 많은 것으로 나타나, 실험 방법에 따라 같은 세포 독성제라도 신혈관 형성 저해 효과 판정이 다르다는 것을 확인시켜 줌과 동시에 향후 thalidomide를 난소암의 보조적 치료제로 이용하는 연구의 필요성을 제시하였다. Objective: Angiogenesis is central role to both the proliferation and metastasis of malignant tumor. The intense interest in angiogenesis has also lead to a re-examination of the activity of established cytotoxic agents which are known to be an antiangiogenic effect anecdotally. In this study, anti-angiogenic effect of cisplatin, paclitaxel and thalidomide was evaluated in human ovarian cancer cell lines and cervical cancer cell line. Methods: Human ovarian cancer cell line A2780, cisplatin resistant human ovarian cancer cell line A2780-CDDP, human breast cancer cell line MCF-7, and squamous cell uterine cervical carcinoma cell line SiHa were used to evaluate the level of mRNA and protein expression of VEGF, bFGF and TSP-1, 2 before and after the treatment with cisplatin, paclitaxel, and thalidomide using RT-PCR, protein extraction, and Western blot. The results were analyzed with Wilcoxon signed rank test in the SAS ver 8.1. Results: Targeted mRNAs were synthesized as 212 bp VEGF, 238 bp bFGF, and 492 bp band sized except mRNA of TSP-2 via RT-PCR. The protein of VEGF and bFGF were appeared as 21KDa and 17 KDa size, however, the protein of TSP-1 was not appeared through western blot. No effect of cisplatin on protein expression was measured in these cell lines, but paclitaxel influenced the expression of bFGF in MCF-7 cell line and the expression of TSP-1 in MCF-7 and SiHa cell lines. TSP-1 expression was influenced by thalidomide in A2780 cell line. The protein expression of VEGF and bFGF were not influenced following treatment with cisplatin, paclitaxel, and thalidomide. Conclusion: These results were suggested that bFGF and TSP-1 will be used as a target gene for the assay of antiangiogenic effect of paclitaxel in breast and uterine cervical cancer tissue and TSP-1 will be used as that of thalidomide in ovarian cancer. Furthermore, thalidomide will be tried as an adjunctive agent for the improvement of the survival in the case of the patient with ovarian cancer.

프로게스테론과 프로게스틴이 인간 제대 정맥 내피세포의 세포 고사에 미치는 영향

류기영(Ki Young Ryu),박영준(Young Joon Park),권희선(Hee Sun Kwon),나영정(Young Jeong Na),조수현(Soo Hyun Cho),조삼현(Sam Hyun Cho),황윤영(Youn Yeung Hwang) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.10

목적 : 폐경 여성에서 호르몬대체요법을 시행함에 있어, 자궁이 있는 경우 프로게스테론 또는 프로게스틴 제재의 첨가는 필수적 사항이다. 그러나 프로게스테론 및 프로게스틴이 혈관에 미치는 영향은 불확실하며, 특히 심혈관계 질환의 예방 효과는 계속 연구 중인 사항이다. 이에 본 연구는 동맥 경화 발생의 초기 단계로 여겨지는 혈관 내피세포의 세포 고사에 미치는 프로게스테론과 프로게스틴의 효과와 에스트로겐과 함께 투여된 프로게스테론과 프로게스틴의 영향을 알아보고자 시행하였다. 연구 방법 : 산부인과적인 합병증이 없는 반복제왕절개술을 시행받는 산모를 대상으로 태아의 분만 직후 무균적으로 탯줄을 채취하여 인간 제대 정맥 내피세포 (Human Umbilical Vein Endothelial Cell: HUVEC)를 획득 하였다. HUVEC은 다음과 같은 9개의 군으로 분류하여 배양하였다. 1군은 배양액만 투여한 대조군, 2군은 Tumor Necrosis Factor-α (TNF-α) 50 ng/ml, 3군은 TNF-α 50 ng/ml과 estradiol (E2) 1×10-7 M을 투여, 4군은 TNF-α 50 ng/ml과 progesterone (P4) 1×10-7 M을 투여, 5군은 TNF-α 50 ng/ml과 medroxyprogesterone acetate (MPA) 1×10-7 M을 투여, 6군은 TNF-α 50 ng/ml과 norethindrone acetate (NETA) 1×10-7 M을 투여, 7군은 TNF-α 50 ng/ml과 E2 1×10-7 M, P4 1×10-7 M을 투여, 8군은 TNF-α 50 ng/ml과 E2 1×10-7 M, MPA 1×10-7 M을 투여, 9군은 TNF-α 50 ng/ml과 E2 1×10-7 M, NETA 1×10-7 M을 투여하였다. HUVEC의 세포 고사의 정도는 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay와 Fluorescence Activated Cell Sorting (FACS) analysis를 통해 알아보았다. 결과 : MTT assay에서 TNF-α는 생존 세포의 비율을 감소시켰으며, 여기에 E2, P4, MPA, NETA를 투여한 경우 생존 세포의 비율이 증가함을 발견하였고, E2와 P4, MPA, NETA 투여군 간에 TNF-α의 영향을 차단하는 효과는 차이가 없었다. 그러나 E2 단독 투여군과 비교하여, E2에 P4, MPA, NETA를 투여한 군에서 그 차단 효과 가 떨어지는 것으로 나타났다. 위 실험에서 나타난 세포사가 세포 고사에 의한 것인지 알아보기 위해 시행한 FACS analysis에서 TNF-α에 24시간 노출되었던 HUVEC 세포는 44.0±2.6%의 세포 고사가 나타났으며, 여기에 E2와 P4, MPA, NETA를 투여한 경우 각각 28.4±2.9, 33.6±3.6, 35.7±1.3, 34.0±3.3%로 통계학적으로 유의하게 (p<0.05) 세포 고사가 감소한 결과를 보여 주었다. 그러나 E2 단독 투여군과 비교하였을 때, MPA 투여군과 E2에 P4, MPA, NETA를 투여한 군에서 36.0±2.5, 36.3±1.9, 37.0±2.0%로 세포 고사의 억제가 E2 단독 투여군 보다는 떨어짐을 알 수 있었다 (p<0.05). 결론 : E2 뿐만 아니라 P4, MPA, NETA 역시 TNF-α에 의해 유도된 세포 고사를 억제하는 것을 알 수 있었지만, E2 단독 투여보다 P4, MPA, NETA를 함께 투여한 경우, HUVEC 세포의 세포 고사를 억제하는 E2의 효과를 감쇠시키는 것으로 판명되었다. 앞으로 더 많은 연구를 통해 에스트로겐과 프로게스테론/프로게스틴 제재의 심혈관계에 미치는 단독 효과 및 상호간의 작용을 평가해야 하고, 이에 본 연구가 초석이 될 것으로 사료되는 바이다. Objective : The addition of progesterone/progestin is mandatory in women with intact uterus for postmenopausal hormone replacement therapy. However, debate is continuing on whether there is an impact of progesterone/progestin on the hormone replacement therapy for cardiac protection. The aim of this study was to investigate the effect of progesterone/progestin and combination with estrogen on the apoptosis of vascular endothelial cells, which has been demonstrated to be an initial step in the development of atherosclerosis. Material and methods : Human Umbilical Vein Endothelial Cells (HUVEC) were cultured. HUVEC apoptosis was induced by Tumor Necrosis Factor-α (TNF-α) (50 ng/ml). 10-7M of estradiol (E2), progesterone (P4), medroxyprogesterone acetate (MPA) and norethindrone acetate (NETA) were added in to the culture media. The percentage of apoptotic HUVEC were measured by 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and Fluorescence Activated Cell Sorting (FACS) analysis. Results : MTT assay demonstrated a significant decrease in percent of survival cells after 24 hours of TNF-α exposure and a reversal of the effect of TNF-α with E2 treatment. P4, MPA and NETA treatment also reversed the effect of TNF-α. The protective effect of E2 and P4 were not different. Compared to the E2 treatment, the percent of survival cell were decreased when P4, MPA and NETA were added to E2 treatment, respectively. Similarly, FACS analysis revealed 44.0±2.6% apoptosis after 24 hours of TNF-α exposure. Treatment with E2 resulted a significant decrease (28.4±2.9%, p<0.05) in apoptosis. P4 (33.6%±2.6%, p<0.05), MPA (35.7±1.3%, p<0.05), NETA (34.0±3.3%, p<0.05) treatment also showed a reduction of cell death. The percent of apoptotic cells between E2 and MPA treatment was significantly different. The addition of P4 (36.0±2.5%), MPA (36.3±1.9%) and NETA (37.0±2.0%) to E2 treatment significantly increased the percent of apoptotic cells compared to those of E2 treatment. Conclusion : These results suggested that not only estradiol, but also progesterone, MPA and NETA inhibited HUVEC apoptosis. But the effect of estrogen on the inhibition of HUVEC apoptosis was attenuated in combination treatment of estrogen and progesterone/progestin.

20세 미만 여성에서의 난소암에 대한 임상적 고찰

김기돈(Kee Don Kim),이정한(Jung Han Lee),민경택(Kyung Taek Min),문영진(Young Jin Moon),김경태(Kyoung Tai Kim),조삼현(Sam Hyun Cho),황윤영(Youn Yeung Hwang),임호준(Ho Jun Lim),이항(Hang Lee) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.2

N/A Objective: The purpose of this study was to review the incidence, types, chemotherapy regimens, operation me#thods, pregnancy rate and propiosis of the ov#arian malignancy under the age of twenty years old. Methods: Retrospective reviews of the medical recordings for 22 patients with ovarian malignancy under the age of twenty years old in the Department of Obstetrics and Gynecology, Hanyang University from 1986 to 1997 were done. Results: Gerin cell tumor accounts for the majority of cases (77%), whereas 5 patients(23%) belong to the common epithelial poup which were all mucinous type. Our experiences with 22 cases are as followings: 5 mucinous cystadenocarcinoma, 8 immature teratoma, 4 endodermal sinus tumor, 3 dysgernma and 2 mixed germ cell tumor. The incidence of this group was 8.2% (22/266) in all ovarian malignancy. Fourteen of them are stage I, each one is, stage II and IV, and six patients are stage III. The average age was 14.9 years old in germ cell tumor and 18.4 yems old in mucinous cystadenoearcinoma. Malignant ovarian cancer under the age of twenty can be treated with conservative surgery, followed by adjuvant chemotherapy. Commonly used chemotherapeutic regimens were VAC(Vincristine, Actinomycin-D; Cyclophosphamide), VBP(Vinblastine, Bleomycin, Cisplatin) and BEP(Bleomycin, Etoposide, Cisplatin). Second look operations were done in 11 patienth and histologic positive findings were detected in 4 of them. The length of follow-up ranged ftom 3 months to 137 months and median value was 44 rnonths. Conclusions: It is concluded that for young women who wish to preserve child-bearing capacity, regardless of the stage of the tumor, fertility preserving surgery with complete surgical staging, if necessary followed by combination chemotherapy is an appropriate treatment.