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Haemophilus influenzae 티올 과산화효소의 특성
황영선(Young Sun Hwang),김강화(Kanghwa Kim) 전남대학교 생활과학연구소 2000 生活科學硏究 Vol.10 No.-
Haemophilus influenzae HI0571 protein that shows homology to E. coli thiol peroxidase uses yeast thioredoxin system as an electron donor[Hwang. Y. S., Chae. H. Z., and Kim. K. (2000) J. Biochem. Mol. Biol. 33, 514~518]. The microorganism has two open, reading frames. HI1158 and HI0084, showing homology to a thioredoxin reductase and a thioredoxin, respectively. Both proteins consisting thioredoxin system were expressed in E. coli, and then the proteins were subsequently purified. The H. influenzae thioredoxin system showed 5.5’-dithiobis-(2-nitrobenzoic acid) reduction activity. H, influenzae HI0751 protein, thiol peroxidase was characterized using the thioredoxin system as an electron donor. The thiol peroxidase showed the thioredoxin dependent peroxidase activity. And glutamine synthetase protection activity of thiol peroxidase against Thiol/Fe<sup>3+</sup>/O₂ system was enhanced by addition of the thioredoxin. An antioxidant function of thiol peroxidase in intact cells was demonstrated by the observation that E. coli cells overexpressed with H. influenzae thiol peroxidase were less sensitive to growth inhibition by alkyl hydroperoxides.
분말형 프로바이오틱스 섭취방법에 따른 구강 내 pH 변화
황영선 ( Young-sun Hwang ),이민경 ( Min-kyung Lee ),김명희 ( Myoung-hee Kim ) 한국치위생학회(구 한국치위생교육학회) 2019 한국치위생학회지 Vol.19 No.6
Objectives: The purpose of this study was to investigate the changes in pH in the oral cavity using the probiotic intake method. Methods: A total of 109 participants were enrolled and randomly assigned to three groups. Participants in the control group did not ingest powdered probiotics, those in experimental group 1 ingested powdered probiotics by dissolving them on the tongue, and those in experimental group 2 dissolved powdered probiotics on the tongue and rinsed with water. pH values were measured 5 times in all. The significance of each group was examined by the Kruskal-Wallis test. The trend over time was expressed as a graph with groupwise means and confidence intervals, considering repeated measurement data. Results: A significant difference was found between the control group and experimental group 1 at two time-points, i.e., immediately after intake and 3 min after ingestion. As a result of the time trend, the pH value of experimental group 2 was smaller than that of experimental group 1, compared to the control group. Conclusions: Studies have shown that taking probiotics with water may help reduce changes in oral pH. Probiotics should be aware of live bacteria and provide consumers with more detailed information on proper dosage and precautions.
황영선 ( Young-sun Hwang ),김기쁨 ( Gi-ppeum Kim ),안경근 ( Kyung-geun Ahn ),김선태 ( Sun Tae Kim ),윤원병 ( Won Byong Yon ),한원영 ( Won Young Han ),정명근 ( Myoung-gun Choung ) 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-
The distribution and variation of the contents of targeted metabolites in soybean [Glycine max (L.) Merr.] seeds are affected by both genetic and environmental factors. In this study, the 192 soybean germplasm accessions collected from two provinces of Korea to elucidate the effects of seed coat color and 100 seeds dry weight on the metabolic variation and responses of targeted metabolites. The main and interaction effects of seed coat color and 100 seeds dry weight were present in sucrose, total oligosaccharides, total carbohydrates and all measured fatty acids. The targeted metabolites were clustered within three groups, 1) daidzein and genistein, 2) glycitein, protein, and linoleic acid, and 3) the rest of the targeted metabolites. These metabolites were not only differently related to 100 seeds dry weight, based on the difference of seed coat color by regression analyses but also responded differentially to seed coat color in principle component analysis(PCA) loading plots. The inter- relationship between the targeted metabolites was highly present in the result of correlation analysis. Overall, results revealed that the targeted metabolites were diverged in relation to seed coat color and 100 seeds dry weight within locally collected soybean seed germplasm accessions.
전력지원체계의 체계적 획득전략 수립을 위한 기술조사·분석 기법의 개발 및 적용
황영선(Yong-Son Hwang),이동헌(Donghun Lee) 한국산학기술학회 2023 한국산학기술학회논문지 Vol.24 No.4
국방과학기술혁신 촉진법의 시행에 따라, 전력지원체계에도 무기체계와 같이 소요기획 및 기술조사·분석 등 시스템 공학에 기반한 체계적인 획득 절차가 정립되었다. 획득이 요구되는 품목 또한 무인 및 자동화 장비, 로봇류 등 4차산업의 발전에 따라 사물인터넷(IoT), 드론, 인공지능(AI) 등이 결합된 장비류로 변화되고 있으며, 이에 따라 기존에 수행되던 기술수준조사의 발전이 요구되고 있다. 본 연구에서는 전력지원체계 기술조사·분석법을 개발하여 기존의 기술수준조사 보다 더 고도화된 연구결과를 제공할 수 있도록 하였다. 이를 위해 MIL-STD-961을 기반으로 전력지원체계 특성에 맞게 군사요구도 항목을 표준화하여 군 요구사항을 분석하고, 대상 품목별 작업분할구조(WBS)와 핵심기능을 이용하여 핵심기술요소(CTE)을 도출하였으며, 하나의 군사요구도만을 제시한 전의 방법과는 다르게 획득시기 및 국내 산업 발전, 기술 수준을 고려한 범위형 군사요구도를 제시하였다. 또한 기술성숙도(TRL) 결과를 분석하여 획득방안을 더 구체화하고 이에 따른 비용분석을 실시하였다. 본 연구에서 제시된 전력지원체계 기술조사·분석법을 적용하여 이전의 기술수준조사 보다 더 체계적인 전력지원체계 획득전략을 수립하고, 군수품 품질 향상에 기여할 수 있을 것으로 기대된다. In combatant support systems, a systematic acquisition procedure based on system engineering was established. Items requiring acquisition are changing into equipment that combines IoT(Internet of Things) and drones. This study developed the technology research and analysis method for the combatant support systems to provide more advanced results than the previous technology-level survey. Based on MIL-STD-961, the list of required operational capability (ROC) was standardized according to the characteristics of the combatant support systems, and the critical technology element (CTE) was derived using the work breakdown structure(WBS) and range type required operational capability (ROC) was presented considering the acquisition period and domestic industrial development. The acquisition method was further specified by analyzing the results of technology readiness level (TRL), and cost analysis was conducted accordingly. This method can help establish a more systematic acquisition strategy of combatant support systems than before and contribute to improving the quality of munitions.
Transcription and Post-Transcription Regulation of Primordial Germ Cell in Chicken
Young Sun Hwang(황영선),Sang In Lee,Bo Ram Lee,Sujung Kim,Deivendran Rengaraj,Min Yoo,Kyung Je Park,Gwonhwa Song,Jae Yong Han 한국가금학회 2010 한국가금학회 정기총회 및 학술발표회 Vol.27 No.-
초기 생식세포 발달은 전사와 전사 후 조절에 의하여 복잡하게 이루어진다. 따라서 mRNA와 miRNA는 발달에 중요한 역할을 할 것이다. 하지만 miRNA가 어떻게 발달과정에 관여하는지는 많이 알려져 있지 않다. 이 연구에서 닭의 6일령 생식선으로부터 분리한 원시생식세포(PGCs)와 생식선 기질세포(GSCs)로부터 RNA를 얻어 microarray 분석을 하였다. 이를 토대로 특정 miRNA가 원시 생식세포 특이적으로 발현하는 것을 알아냈고, 또한 이 miRNA가 표적 mRNA의 3' 비번역영역(Untranslated reigon)에 결합하여 유전자의 발현을 억제한다는 것을 확인하였다. 이러한 결과는 원시생식세포 특이적 mRNA나 miRNA가 발생에 중요한 역할을 하며 이를 이해하는데 기여할 것이다.
연구보문 : 환경화학 ; GC-ECD/MS를 이용한 농산물 중 Captan, Folpet, Captafol 및 Chlorothalonil의 잔류분석법
이수진 ( Su Jin Lee ),황영선 ( Young Sun Hwang ),김영학 ( Young Hak Kim ),권찬혁 ( Chan Hyeok Kwon ),도정아 ( Jung A Do ),임무혁 ( Moo Hyeog Im ),이영득 ( Young Deuk Lee ),정명근 ( Myoung Gun Choung ) 한국환경농학회 2010 한국환경농학회지 Vol.29 No.2
A gas chromatographic (GC) method was developed to determine residues of captan, folpet, captafol, and chlorothalonil, known as broad-spectrum protective fungicides for the official purpose. All the fungicide residues were extracted with acetone containing 3% phosphoric acid from representative samples of five agricultural products which comprised rice, soybean, apple, pepper, and cabbage. The extract was diluted with saline, and dichloromethane partition was followed to recover the fungicides from the aqueous phase. Florisil column chromatography was additionally employed for final cleanup of the extracts. The analytes were then determined by gas chromatography using a DB-1 capillary column with electron capture detection. Reproducibility in quantitation was largely enhanced by minimization of adsorption or thermal degradation of analytes during GLC analysis. Mean recoveries generated from each crop sample fortified at two levels in triplicate ranged from 89.0~113.7%. Relative standard deviations (RSD) were all less than 10%, irrespective sample types and fortification levels. As no interference was found in any samples, limit of quantitation (LOQ) was estimated to be 0.008 mg/kg for the analytes except showing higher sensitivity of 0.002 mg/kg for chlorothalonil. GC/Mass spectrometric method using selected-ion monitoring technique was also provided to confirm the suspected residues. The proposed method was reproducible and sensitive enough to determine the residues of captan, folpet, captafol, and chlorothalonil in agricultural commodities for routine analysis.