Isolation and Expression Analysis of Brassica rapa WRKY 7

황덕주,김선철,고유진,장지영,이데레사,임명호,박상렬,배신철,윤충효,박범석 한국식물병리학회 2008 Plant Pathology Journal Vol.24 No.4

The cDNA clone of Brassica rapa WRKY7 (BrWRKY7) was obtained from EST collection in Brassica genomics team and its DNA sequence was determined. The cDNA clone is 1,037 bp long in nucleotides and encodes an open reading frame of 307 amino acids. Based on a phylogenetic tree, BrWRKY7 belongs to group IId. BrWRKY7 was induced by wound and SA. It was also induced by pathogen attack such as Xanthomonas campestris pv. campestris (Xcc), suggesting that this BrWRKY may play an essential role in defense response of chinese cabbages.

Zymomonas mobilis 의 Plasmid Vector 제조에 관한 연구

황덕주(Duk - Ju Hwang),이상기(Sang - Ki Rheer),박무영(Moo - Young Park) 한국미생물생명공학회 1987 한국미생물·생명공학회지 Vol.15 No.5

PCR방법을 이용한 국화흰녹병균 검출

전푸름,박상렬,김정구,안일평,황덕주,배신철 한국화훼산업육성협회 2014 화훼연구 Vol.22 No.4

우리나라의 절화국화는 국내 화훼작물 중 큰 비중을 차 지하고 있다. 수출 또한 2011년에는 2001년대비 약 1.9배 증가하였지만 생산단가 상승과 주변 경쟁국들과의 가격 경쟁력으로 수출농가가 어려움을 겪고 있다. 이를 극복하기 위해서는 소비자의 기회에 맞는 품종을 개발하 고 또한 고품질 국화를 생산하는 것이 필요하다. 국화 품 질을 저하시키는 요인 중 하나는 병해충 감염이며, 국화 흰녹병이 가장 심각한 피해를 주고 있다. 국화흰녹병 병 발생을 근절할 수 있는 근본적인 해결책은 저항성 품종 개발이지만 이는 많은 시간이 필요하기에 단기적 방법으 로 가시적으로 병징이 나타나기 이전에 국화흰녹병 감염 여부를 검정할 수 있는 기술을 개발한다면 병 발생 피 해를 최소화할 수 있을 것이다. 국화흰녹병 검정 기술 개 발을 위해 실험실 조건에서 이병주의 연중 생산이 필수 적이기에 이병주를 생산하는 시스템을 우선적으로 확립 하였다. 그리고 자체 생산한 이병주에서 증식된 병원균 의 담자포자의 형태적 특성 및 rDNA의 DNA 염기서열 분석 결과로 국화흰녹병균임을 입증하였다. 또한 국화흰 녹병균 유전체 정보를 이용하여 국화흰녹병균만을 검출 할 수 있는 유전자를 선발하였고 이 유전자를 대상으로 국화흰녹병균 게놈 DNA 0.25ng까지 검출할 수 있는 프 라이머 쌍을 개발하였다. 동일한 프라이머를 이용하여 Real-time PCR를 실시하면 게놈 DNA 6pg까지 검출이 가능하였다.향후 이러한 방법을 이용하여 병원균 접종후 육안으로 병징이 확인되기 이전에 국화흰녹병균의 검출 을 확인할 예정이다. In South Korea, chrysanthemum cut flowers have a large share in the flower industry and, compared to 2001, the export of chrysanthemum cut flower increased by 1.9 times in 2011. However, Korean growers have faced with difficulty in keeping competitiveness in world chrysanthemum cut flower market due to increase production cost and selling price competition. In order to overcome these problems, it is needed to develop cultivars that consumers demand and to produce cut flower chrysanthemum with high quality. Diseases and insects can cause severe quality problem of cut flower chrysanthemum. The most serious damage is caused by chrysanthemum white rust. The best option to minimize damage caused by white rust is to develop cultivars resistant to chrysanthemum white rust, but it will take so long time to develop a resistant cultivar. Therefore, an alternative way can be development of technique for early detection of infection by chrysanthemum white rust, which can minimize damage of cut flower chrysanthemum. We preferentially established the system for production of white rust susceptible chrysanthemum plants because it is required to supply susceptible plants in laboratories throughout the year to develop the detection technique for white rust. Pathogens sampled from susceptible plants were identified as chrysanthemum white rust through investigation of shape and size of basidiospore using microscope and analysis of rDNA sequence of the pathogen. In addition, the specific gene for chrysanthemum white rust was selected from genome database of Puccinia horiana and 0.25 ng of its genomic DNA could be obtained by PCR using primers designed to target the specific gene of chrysanthemum white rust. Six pg of its genomic DNA could be also amplified by Real-time PCR. In the near future, we will confirm if chrysanthemum white rust can be detected through the PCR method mentioned above before symptom caused by infection of white rust is become visible.

대두 철분결합단백질 유전자 발현 형질전환 감자의 감자무름병 방어 증진효과

배신철,황덕주,고승주,변명옥,허성기,여윤수 한국식물생명공학회 2002 JOURNAL OF PLANT BIOTECHNOLOGY Vol.29 No.4

Expression Analysis of the Metacaspase Gene Family in Arabidopsis

권순일,황덕주 한국식물학회 2013 Journal of Plant Biology Vol.56 No.6

Metacaspase is discovered gene family with distantcaspase homologs in protozoa, fungi, and plants. TheArabidopsis genome has nine metacaspase genes. Based onthe presence or absence of an N-terminal domain, they canbe divided into two types of metacaspase genes: type I(AtMC1 to 3) and type II (AtMC4 to 9). To examine theexpression patterns of the Arabidopsis metacaspase genefamily, we measured the levels of expression of themetacaspase genes in various tissues and during bioticstresses by quantitative reverse transcription-polymerasechain reaction. As expected, the nine AtMC genes wereexpressed differently among tissues and during diverse stressconditions. In addition, to address the spatial expression ofAtMC genes, their expression patterns using the promoterregion of AtMCs (0.85 kb to 2.55 kb) were examined by theGUS reporter system. By observing GUS activity intransgenic plants containing nine AtMCpro::GUS constructs,we found that expression of these nine AtMC genes wasregulated developmentally and spatially. We also observedthat AtMC::green fluorescent fusion proteins were mainlylocalized in the nucleus using the Arabidopsis protoplasttransient assay. In summary, our data suggest that AtMCsgene family be involved in various defense responses duringbiotic stresses. Keywords: Cell

Evaluation of Fusarium Head Blight in Barley Infected by Fusarium graminearum

강우리,황덕주,배신철,이데레사,Soonok Kim,안일평 한국미생물학회 2013 The journal of microbiology Vol.51 No.4

Fusarium head blight, which is primarily caused by Fusarium graminearum, is a devastating disease in the barley field. A real-time PCR protocol was developed to evaluate the growth of this pathogen in the host plant tissues. All four strains harbored the gene encoding ATP-BINDING CASSETTE TRANSPORTER (FgABC; FGSG_00541) as a single copy within their genomes. Our Southern blot result was identical with the genomic data for F. graminearum strain PH-1. Based on the crossing point (CP) values obtained in our TaqMan real-time PCR analysis, two standard curves describing the relationship among the CP value, FgABC copy number, and amount of fungal DNA were constructed. Chronological enumeration of fungal growth was coincided with the symptom development.

무름병에 감수성인 애기장대 돌연변이체 Atstp1 선발

최창현,황덕주,김민갑,안일평,박상렬,배신철 한국식물병리학회 2010 식물병연구 Vol.16 No.3

Pectobacterium carotovorum subsp. carotovorum (Pcc) causes soft rot disease in various plants. Although many studies about Pcc have been going on, little is known yet about the defense genes from plants. To identify defense associated genes in response to Pcc, we screened about 20 thousand Arabidopsis T-DNA knock out lines by inoculation with Pcc. We obtained a line (Atspt1) showing more susceptible symptom compared to WT (Col-0) on 1 day after the inoculation of Pcc on leaves of Arabidopsis with toothpicks. In this study, we optimized the system to select resistant and susceptible lines to Pcc from T-DNA inserted pool of Arabidopsis and expect the system and Atspt1 might be used for molecular breeding to produce resistant vegetables against Pcc. 본 연구는 애기장대에서 무름병에 대한 저항성 유전자를 탐색하고자 2만여개의 T-DNA 삽입 돌연변이군을 이용하여 Pcc에 대한 스크리닝을 수행하고 이 방법을 소개한 연구다. 1차 선발을 통하여 15개의 저항성 line과 20개의 감수성 line을 선발하였으며, 이로부터 2차 선발하여 3개의 저항성 line과 4개의 감수성 line을 선발하였고, 최종적으로 3차 선발을 통하여 1개의 감수성 line (Atstp1)을 선발할 수 있었다. 현재 Atstp1을 이용해 flanking sequencing 하여 유전자를 탐색하고 있으며, 앞으로 클로닝을 통하여 다양한 무름병 저항성 식물 개발에 유용하게 이용될 것으로 기대한다.

국화 흰녹병균 생태 및 생명공학적 진단에 대한 연구동향

박상렬,안일평,황덕주,장안철,임기병,배신철 한국화훼산업육성협회 2013 화훼연구 Vol.21 No.3

Chrysanthemum is an economically important horticultural crop in chrysanthemum-growing areas including Korea. White rust is the most dangerous disease caused by an obligate pathogen Puccinia horiana. Control of white rust is dependent on chemical treatment and other cultural practices. However, chemical control of disease results in appearance of resistant race of white rust pathogen. Hence, development of chrysanthemum cultivar resistant to white rust is the most desirable. Nevertheless, not much research has been done about white rust pathogen (e.g infection process, pathogen diversity, etc). In order to investigate the way of chrysanthemum to overcome white rust we reviewed literatures reported about white rust pathogen, especially in terms of disease/life cycle and ecology of P. horiana and disease assessment based on polymerase chain reaction in this study. This review will be helpful for disease control and molecular breeding of chrysanthemum resistant to white rust. 국화는 국내외적으로 주요 화훼작물이지만 국화 흰녹병은 안정적 생산에 큰 걸림돌이 되고 있다. 현재까지 국화 흰녹병 방제는 농약살포를 통한 화학적 방제와 다습한 재배환경을 개선하는 재배적 방제에 의존하고 있지만, 살균제 저항성을 갖는 새로운 병원균 출현으로 농약 살포를 통한 병 방제에는 한계가 있어 병 저항성 품종 개발이 절실하다. 흰녹병은 순활물기생균인 Puccinia horiana에 의해 발생하는 곰팡이 병으로 이로 인한 경제적 손실이 크지만, 다른 주요 작물병과 비교하여 상대적으로 연구가 미흡하다. 특히 병원균의 전파 경로와 병원성의 다양성(pathogenic diversity), 병원균 침입기작 및 기주식물과의 상호작용에 대한 전반적인 지식 부족으로 효과적 농약 살포 기준 설정과 병저항성 품종개발에 어려움이 있다. 따라서 본 논문에서는 이러한 과제를 해결하는데 기초자료를 제공하고자 각 연구분야별로 현재까지 발표된 연구결과를 정리하고 앞으로 요구되는 연구과제에 대하여 모색하여 보았다. 특히 효과적 약제처리 기준 설정과 병저항성 품종 개발의 출발점이 되는 흰녹병균의 생활사와 생태, 병원균 DNA 정보를 이용한 조기진단, 병원균의 병원형 등에 관해 현재까지의 연구결과들을 정리하였다. 앞으로 흰녹병균의 식물체 침입 기작에 관한 종합적 이해의 폭을 넓히고, 병원균 침입 시 식물체내에서 발병과 병저항성 유도 요인에 대한 분자 수준에서 연구가 진행된다면 흰녹병균 저항성 품종 육성 시기를 앞당길 수 있으리라 사료된다.

The WRKY Superfamily of Rice Transcription Factors

장지영,최창현,황덕주 한국식물병리학회 2010 Plant Pathology Journal Vol.26 No.2

WRKY transcription factors are known to be involved in many different biological processes including plant response to biotic stress, abiotic stress, and plant development. WRKY proteins are extensively studied in Arabidopsis. Recently, reports on WRKY proteins are rapidly increasing in the other plant species, especially in rice. Therefore, this review will discuss the function of rice WRKY proteins reported so far.

광범위 병저항성 유전자발굴 연구동향

안일평,박상렬,황덕주,장안철,배신철 한국육종학회 2012 한국육종학회지 Vol.44 No.4

Disease is one of the devastating obstacles in the stable crop production. Numerous agronomical and chemical controls have been developed to overcome this problem, but the former is not sufficient for the maintaining the disease under the economic threshold level and the latter is not free from the environmental regulation. One of the most ideal solutions is resistance breeding. Resistance breeding has been majorly dependent on the resistance (R) genes conferring race-specific vertical resistance effective for limited populations within the pathogen species harboring avirulence (Avr) genes encoding effectors exactly matching with R gene products. In spite of its outstanding efficiency, improper management of above cultivars frequently resulted in the resistance break down due to the appearance and domination of the new races in the field. During the last two decades, mechanisms of disease resistance have been characterized and analyzed in the respect of genetics, biochemistry, molecular biology, cell biology, and evolution. Especially, a growing body of investigations has been focused on the resistance effective for multiple races or even more species of pathogen’s infections and also durable and long-lasting. In this manuscript, we will introduce the investigations searching for durable and broad-spectrum resistance and the considerations for their applications in the crop production will be presented.