RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기

      선택해제
      • 좁혀본 항목 보기순서

        • 원문유무
        • 원문제공처
          펼치기
        • 등재정보
        • 학술지명
          펼치기
        • 주제분류
        • 발행연도
          펼치기
        • 작성언어
        • 저자
          펼치기

      오늘 본 자료

      • 오늘 본 자료가 없습니다.
      더보기
      • 무료
      • 기관 내 무료
      • 유료
      • KCI등재

        미곡종합처리장 곡류시료 내 곰팡이 오염과 Fusarium 독소 발생

        이데레사,이수형,김이한,류재기 한국식물병리학회 2014 식물병연구 Vol.20 No.4

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 9시까지 원문보기가 가능합니다.

        Rice samples including paddy, husk, brown rice, blue-tinged rice, broken rice, discolored rice and polished ricewere collected from rice processing complexes(RPC) nationwide to determine the contamination of fungi andmycotoxins on rice during 2010-2013. Among the samples, paddy rice had the highest frequencies of fungaland Fusarium occurrence, and the frequencies decreased along with milling as husk was the next. Blue-tingedrice or discolored rice was similar with brown rice for fungal occurrence, and polished rice showed the lowestfrequency. Among Fusarium species, F. graminearum species complex occupied 87% in 2012 but did 35-39%in 2011 and 2013. Aspergillus and Penicillium species appeared at low frequency in most samples but occurredat higher frequency in certain RPC samples. Alternaria, Nigrospora, and Epicoccum species occurred similarlyto the pattern of total fungi. The rice samples from 2010-2012 were analyzed for the occurrence of Fusariummycotoxins including deoxynivalenol, nivalenol, zearalenone, and other trichothecenes. The most highly contaminatedsample was discolored rice in terms of frequency, level, ratio of simultaneous contamination withmultiple toxins, followed by blue-tinged rice. Rice samples including paddy, husk, brown rice, blue-tinged rice, broken rice, discolored rice and polished rice were collected from rice processing complexes(RPC) nationwide to determine the contamination of fungi and mycotoxins on rice during 2010-2013. Among the samples, paddy rice had the highest frequencies of fungal and Fusarium occurrence, and the frequencies decreased along with milling as husk was the next. Blue-tingedrice or discolored rice was similar with brown rice for fungal occurrence, and polished rice showed the lowestfrequency. Among Fusarium species, F. graminearum species complex occupied 87% in 2012 but did 35-39%in 2011 and 2013. Aspergillus and Penicillium species appeared at low frequency in most samples but occurredat higher frequency in certain RPC samples. Alternaria, Nigrospora, and Epicoccum species occurred similarlyto the pattern of total fungi. The rice samples from 2010-2012 were analyzed for the occurrence of Fusariummycotoxins including deoxynivalenol, nivalenol, zearalenone, and other trichothecenes. The most highly contaminatedsample was discolored rice in terms of frequency, level, ratio of simultaneous contamination withmultiple toxins, followed by blue-tinged rice.

      • KCI등재

        후자린산(Fusaric acid) 생성 Fusarium 종의 신속 검출 PCR

        이데레사,김소수,함현희,이수형,홍성기,류재기,Lee, Theresa,Kim, Sosoo,Busman, Mark,Proctor, Robert H.,Ham, Hyeonhui,Lee, Soohyung,Hong, Sung Kee,Ryu, Jae-Gee 한국식물병리학회 2015 식물병연구 Vol.21 No.4

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 9시까지 원문보기가 가능합니다.

        후자린산은 Fusarium이 생성하는 독소로서 다른 Fusarium 독소와 함께 독성을 증진시킬 수 있다. 후자린산 독소를 특이적으로 검출하기 위해 후자린산의 생합성유전자 중 전사인자인 FUB10을 증폭하는 프라이머를 제작하였다. Fub10-f와 Fub10-r 프라이머쌍으로 PCR을 수행했을 때, F. oxysporum, F. proliferatum, F. verticillioides, F. anthophilum, F. bulbicola, F. circinatum, F. fujikuroi, F. redolens, F. sacchari, F. subglutinans, F. thapsinum에서 약 550 bp의 단일밴드가 증폭되었으며 이들은 모두 후자린산을 생성하는 것으로 알려졌다. 반면 트라이코쎄신을 생성하는 종에서는 FUB10 특이 밴드가 증폭되지 않았다. 후자린산은 푸모니신을 생성하는 종에서 함께 생성될 수 있기 때문에 FUB10 프라이머와 푸모니신 생합성유전자인 FUM1 프라이머를 이용한 multiplex PCR을 수행하였다. 그 결과 푸모니신 생성종인 F. proliferatum과 F. verticillioides에서 밴드가 모두 증폭되었으며 이는 두 가지 독소를 생성할 수 있는 종에서 동시 검출이 가능함을 시사하였다. Fusaric acid is a mycotoxin produced by species of the fungus Fusarium and can act synergistically with other Fusarium toxins. In order to develop a specific detection method for fusaric acid-producing fungus, PCR primers were designed to amplify FUB10, a transcription factor gene in fusaric acid biosynthetic gene cluster. When PCR with Fub10-f and Fub10-r was performed, a single band (~550 bp) was amplified from F. oxysporum, F. proliferatum, F. verticillioides, F. anthophilum, F. bulbicola, F. circinatum, F. fujikuroi, F. redolens, F. sacchari, F. subglutinans, and F. thapsinum, all of which were known for fusaric acid production. Whereas the FUB10 specific band was not amplified from Fusarium species known to be trichothecene producer. Because production of fusaric acid can co-occur in species that also produce fumonisin mycotoxins, we developed a multiplex PCR assay using the FUB10 primers as well as primers for the fumonisin biosynthetic gene FUM1. The assay yielded amplicons from fumonisin producers such as F. proliferatum and F. verticillioides, allowing for the simultaneous detection of species with the genetic potential to produce both types of mycotoxins.

      • KCI등재

        Comparison of Trichothecene Biosynthetic Gene Expression between Fusarium graminearum and Fusarium asiaticum

        이데레사,류재기,이승호,신진영,김희경,윤성환,김황용,이수형 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.1

        Nivalenol (NIV) and deoxynivalenol (DON) are predominantFusarium-producing mycotoxins found ingrains, which are mainly produced by Fusarium asiaticumand F. graminearum. NIV is found in most of cerealsgrown in Korea, but the genetic basis for NIV productionby F. asiaticum has not been extensively explored. In thisstudy, 12 genes belonging to the trichothecene biosyntheticgene cluster were compared at the transcriptional levelbetween two NIV-producing F. asiaticum and four DONproducingF. graminearum strains. Chemical analysisrevealed that time-course toxin production patterns over14 days did not differ between NIV and DON strains,excluding F. asiaticum R308, which was a low NIVproducer. Both quantitative real-time polymerase chainreaction and Northern analysis revealed that the majorityof TRI gene transcripts peaked at day 2 in both NIV andDON producers, which is 2 days earlier than trichotheceneaccumulation in liquid medium. Comparison of the geneexpression profiles identified an NIV-specific pattern intwo transcription factor-encoding TRI genes (TRI6 andTRI10) and TRI101, which showed two gene expressionpeaks during both the early and late incubation periods. In addition, the amount of trichothecenes produced byboth DON and NIV producers were correlated withthe expression levels of TRI genes, regardless of the trichothecenechemotypes. Therefore, the reduced productionof NIV by R308 compared to NIV or DON by the otherstrains may be attributable to the significantly lowerexpression levels of the TRI genes, which showed earlyexpression patterns.

      • KCI등재

        Restriction Enzyme-Mediated Integration 방법으로 확보한Fusarium oxysporum 형질전환체의 후자리산 생성능 분석

        이데레사,신진영,손승완,이수형,류재기 한국식물병리학회 2013 식물병연구 Vol.19 No.4

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 9시까지 원문보기가 가능합니다.

        Fusaric acid (FA) is a mycotoxin produced by Fusarium species. Its toxicity is relatively low but oftenassociated with other mycotoxins, thus enhancing total toxicity. To date, biosynthetic genes or enzymes for FAhave not been identified in F. oxysporum. In order to explore the genetic element(s) for FA biosynthesis,restriction enzyme mediated integration (REMI) procedure as an insertional mutagenesis was employedusing FA producing-F. oxysporum strains. Genetic transformation of two F. oxysporum strains by REMIyielded more than 7,100 transformants with efficiency of average 3.2 transformants/μg DNA. To develop ascreening system using phytotoxicity of FA, eleven various grains and vegetable seeds were tested forgermination in cultures containing FA: Kimchi cabbage seed was selected as the most sensitive host. Screening for FA non-producer of F. oxysporum was done by growing each fungal REMI transformant inCzapek-Dox broth for 3 weeks at 25oC then observing if the Kimchi cabbage seeds germinated in the culturefiltrate. Of more than 5,000 REMI transformants screened, fifty-three made the seeds germinated, indicatingthat they produced little or fewer FA. Among them, twenty-six were analyzed for FA production by HPLCand two turned out to produce less than 1% of FA produced by a wild type strain. Sequencing of genomicDNA regions (252 bp) flanking the vector insertion site revealed an uncharacterized genomic regionhomologous (93%) to the F. fujikuroi genome. Further study is necessary to determine if the vector insertionsites in FA-deficient mutants are associated with FA production. 후자린산(FA)는 Fusarium 속 균이 생성하는 독소로서다른 곰팡이독소보다 독성은 낮으나 다른 독소와 중복 오염시 전체 독성을 증진시키는 것으로 알려졌다. 현재까지FA 생합성 관련 효소나 유전자가 Fusarium oxysporum에서 밝혀지지 않았기 때문에 본 연구에서는 관련 생합성유전자의 발굴을 위해 제한효소를 통한 무작위 삽입 형질전환방법인 REMI를 이용하여 FA 생성 F. oxysporum균주의 생합성유전자의 결손을 시도하였다. F. oxysporum균주 2주를 대상으로 REMI를 시도한 결과, 평균 3.2주(1 μg DNA 당)의 효율로 7,100주 이상의 형질전환체를육성하였다. FA 미생성 형질전환체를 스크리닝 하기 위해 FA가 함유된 배양액에서 다양한 식물종자의 발아여부를 조사한 결과, 11종의 종자 중 가장 감수성인 배추종자를 선발하였다. 각 형질전환체는 Czapek-Dox broth에서3주간 배양한 후 배양여액을 배추종자의 발아여부 검정에 사용하였다. 검정결과 총 5,000여 주의 REMI 형질전환체 중 53주의 배양여액에서 종자가 발아하지 않아, 이들을 FA 미(저) 생성 추정 형질전환체로 선발하였다. 이중 26주의 FA 생성량을 HPLC로 분석한 결과, 2주의 형질전환체에서 모균주 생성량의 1% 이하의 FA가 검출되었다. 이 중 형질전환체 1주로부터 REMI 벡터 삽입 부위 게놈 DNA의 염기서열(252 bp)을 확보하였으며, 이 부위는 F. fujikuroi의 미동정 게놈부위와 93% 유사성이 있음을 확인하였다. 이 부위의 FA생성 관련성 증명을 위해서는 추후 연구가 필요하다.

      • KCI등재

        벼 붉은곰팡이병 감염에 대한 기상조건의 영향과 장려품종의 반응

        이데레사,장자영,김점순,류재기 한국식물병리학회 2018 식물병연구 Vol.24 No.4

        Fusarium infection rate of the paddy rice grain after harvest seemed to be influenced by the average temperature from late July (before heading) to the end of September (during ripening). In case of 2010 and 2013 in which average temperature of the same period was similar, Fusarium infection was related to cumulative precipitation, cumulative precipitation days, and precipitation durations over two days. The distribution ratio of Fusarium species complex isolated from paddy rice grains after harvest was 57% in 2010 and 45% in 2013 for Fusarium graminearum species complex (FGSC), 35% and 50% for Fusarium incarnatum-equiseti species complex, and 8% and 5% for Fusarium fujikuroi species complex (FFSC). The distribution ratios of FGSC and FFSC were higher in 2010 than 2013. Among the total 26 promoted rice varieties, the ‘Mihyang’ showed resistant response against the natural infection with Fusarium species belonging to FGSC and the varieties of ‘Nampyeong’, ‘Hi-ami’and ‘Younghojinmi’ showed resistant response against the natural infection with overall Fusarium pathogens. Majority of the promoted rice varieties could not be classified for resistance or susceptibility. These results are valuable as basic data to determine the resistance and susceptibility of rice variety against Fusarium spp. infection in the field.

      • KCI등재

        국내산 미곡에 발생하는 곰팡이와 곰팡이독소

        이데레사,이수형,이정화,윤종철,오경석 한국식물병리학회 2012 식물병연구 Vol.18 No.4

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 9시까지 원문보기가 가능합니다.

        Inspection of deteriorated rices in Korea for fungal occurrence revealed that Aspergillus was the most frequently observed genus and some isolates of the Aspergillus spp. turned out to produce aflatoxin. Diverse fungal genera including Fusarium, Aspergillus, Penicillium, or Alternaria spp. were observed in most of the rice samples. Aflatoxin occurred infrequently and the levels of aflatoxin present in the rice samples were lower than regulatory limit but Fusarium toxins such as deoxynivalenol, nivalenol, zearalenone, and fumonisin occurred frequently. In rice processing complexes, fungal and mycotoxin contamination of rice decreased by milling process, resulting in the lowest level of mycotoxin and fungi in polished rice. Currently, it appears that Korean rice and milled by-products need a safety control for Fusarium toxins rather than aflatoxin.

      • KCI등재

        공정무역 커피의 가치평가 결정요인과 지불의사금액의 추정

        이데레사,이희찬 한국외식산업학회 2019 한국외식산업학회지 Vol.15 No.2

        The purpose of study is to analyze the determinants of economic value estimation and valuation of Fair Trade Coffee in Korea. As an analytical method, Double Bounded Dichotomous Choice(DBDC) of Contingent Valuation Method (CVM) was applied using 521 samples from Seoul and metropolitan area. As a result of estimating respondents payment amount by the Willingness to Pay(WTP) truncated mean, consumers are willingness to pay(WTP) an additional 3,024 KRW for Fair Trade Coffee. The determinants that affect willingness to pay are the value-oriented propensity to consume, the individuality-oriented propensity to consume, the Involvement, and the Level of Knowledge. That is they are consumers who are willing to pay more if the quality of products is recognized, or if the products are differentiated. On the other hand, social-economical attributes do not have effect on willing to pay.

      • KCI등재

        시판 건조 인삼류(백삼, 홍삼)의 곰팡이 발생

        이데레사,최장남,김소수,백슬기,박진주,최정혜,장자영,김점순 한국식품위생안전성학회 2022 한국식품위생안전성학회지 Vol.37 No.4

        To investigate the occurrence of fungi in dried ginseng products, we collected 24 white and 26 red ginseng samples from the retail market. Fungi were detected in 50% and 46% of white and red ginseng samples, respectively. The average level of fungal contamination was 0.5 and 0.2 log10 CFU/g in white and red ginseng, respectively. In white ginseng, Penicillium polonicum, P. chrysogenum, and Rhizopus microsporus dominated with each having an occurrence of 18.2%. In red ginseng, Aspergillus spp. was dominant with an occurrence of 87.6%, with A. chevalieri having the highest occurrence (50%). PCR screening for mycotoxigenic potential showed that the 13 isolates of 4 species (P. polonicum, P. chrysogenum, P. melanoconidium, and A. chevalieri) tested were negative for the citrinin biosynthetic gene. These results show that the samples tested in this study had low risk of mycotoxin contamination. However, there is a possibility of dried ginseng products, such as white and red ginseng, being contaminated with fungi. 시판 유통 중인 건조 인삼류(백삼 24점, 홍삼 26점) 포장 제품을 수집하여 곰팡이 발생 조사를 수행하였다. 백삼과 홍삼은 각각 50%와 46%의 시료에서 곰팡이가 검출되었고 검출 시료의 평균 곰팡이 오염도는 각각 0.5와 0.2 log10 CFU/g이었다. 백삼에서는 Penicillium polonicum, P. chrysogenum, Rhizopus microsporus가 각각 18.2%로 우점하였으나 홍삼은 Aspergillus spp.이 87.6%로 우점하였으며 이중 A. chevalieri가 50.0%로 가장 높았다. 이 중 독성 종으로알려진 P. polonicum, P. chrysogenum, P. melanoconidium, A. chevalieri 균주의 citrinin 독소 생성 가능성을 분석한 결과, 13 균주 모두 독소 유전자가 검출되지 않았다. 이 결과는 조사한 시료의 곰팡이독소 오염 위험은 매우 낮지만, 건조 인삼류에 곰팡이 오염이 가능함을 보여준다.

      • KCI등재

        로스터리 커피전문점 바리스타의 역량이 관계품질, 재방문의도에 미치는 영향

        이데레사,이애주 한국외식산업학회 2018 한국외식산업학회지 Vol.14 No.2

        With the coffee market becoming bigger, consumer level has gone up. This tendency provoked the argument that the coffee market should grow not only in terms of quantity but also in terms of quality, and much attention has been given toprofessional baristas equipped with expertise and experience. It is indispensable for businesses concerned to research into the correlation between barista's capability and relationship quality and set marketing strategies considering the outcomes of research. Even though this experience is significant, a barista’s specialized knowledge must be scientifically proved. Without experts putting efforts on the growth of the overall quality in coffee, coffee industries are unlikely to continue their success. In addition, a customer’s evaluation on a barista’s competence makes his image. The latent attributes reflects in customer satisfaction, trust, and revisit intention, it is fundamental element in marketing to actively respond to a customer’s needs and competitive advantage strategy. This paper attempts to find out correlations between 'barista's capability', 'relation quality' and 'revisit intention'. Analysis was done on how these factors influence one another and research was done to verify a hypothesis on the mediation effect of barista' capability on revisit intention.

      • KCI등재

        겉보리의 저장온도와 수분함량이 붉은곰팡이병균과 곰팡이독소 오염에 미치는 영향

        함현희,이경아,이데레사,한상현,홍성기,이수형,류재기 한국식품위생안전성학회 2017 한국식품위생안전성학회지 Vol.32 No.4

        Fusarium graminearum is a mycotoxigenic plant pathogen, which could remain in harvested barley grains and produces mycotoxins at preferable conditions during storage. To elucidate the factors affecting contamination of Fusarium and Fusariotoxin in hulled barley during storage, three hulled barley grain samples were collected from Jeolla province. Moisture content of each sample was adjusted to 14% and 20%, respectively, then stored in two warehouses where temperature was controlled differently: one controlled below 12oC, and the other with no control. While monitoring temperature and relative humidity of warehouses hourly, grain moisture content, Fusarium occurrence, and mycotoxin level was analyzed at 1, 3, 6, and 12 month after storage. The average monthly temperature and relative humidity ranged 3~29oC, and 58~70% in warehouse without temperature control, whereas 3~13oC and 62~ 74% in warehouse controlled below 12oC. Grain moisture content of the samples decreased in both warehouses except 14% samples which increased in the warehouse with temperature control. Fusarium frequency of the contaminated grains decreased continuously in the warehouse without temperature control. But in the warehouse below 12oC, Fusarium decreasing rate was slower because of high grain moisture content. In most samples, nivalenol was detected more in the warehouse without temperature control after 12 month but there was little difference after 1, 3, and 6 month. Therefore, it will be efficient to store hulled barley in the warehouse controlled below 12oC to reduce Fusarium contamination when the barley is not dried properly. In addition, when storage period exceeds 12 month, it is recommended to store hulled barley in a warehouse controlled below 12oC to reduce nivalenol contamination.

      연관 검색어 추천

      이 검색어로 많이 본 자료

      활용도 높은 자료

      해외이동버튼