인천지역 설사환자에서 분리한 Salmonella enterica serovar Enteritidis의 항생제 내성 및 다제내성 양상

황경화,오보영,김정희,김명희,제갈승,이은주,이은정,조남규,고종명,김용희,Hwang, Kyoung-Wha,Oh, Bo-Young,Kim, Jung-Hee,Kim, Myeong-Hee,JeGal, Seung,Lee, Eun-Ju,Lee, Eun-Jung,Cho, Nam-Kyu,Go, Jong-Myoung,Kim, Yong-Hee 한국미생물학회 2009 미생물학회지 Vol.45 No.2

본 연구에서는 2004년 1월부터 2008년 12월까지 인천지역 5개 종합 병 의원에 설사증상으로 입원 또는 내원한 환자의 대변에서 분리한 115주의 S. Enteritidis의 항생제 내성 및 다제내성 양상을 모니터링하였다. 그 결과, 가장 내성율이 높은 항생제는 nalidixic acid (54.8%)이었고, ampicillin (40.0%), ticarcillin (38.3%), chloramphenicol (20.9%), tetracycline (17.4%) 순이었다. 분리균의 91.3%는 1제 이상의 항생제에 내성이 있었다. 항생제 내성 유형을 살펴본 결과 nalidixic acid 단일 내성이 44.3%로 가장 높았으며, ampicillin-chloramphenicol-ticarcillin (11.3%) 및 ampicillin-ticarcillin (10.4%) 순이었다. 전체적으로 1제 내성이 51.9%, 2제 내성 18.2%, 3제 내성 14.5%, 4제 내성 5.8% 그리고 5제 이상 내성은 9.7%이었다. This study describes the antimicrobial susceptibility and multidrug resistance patterns in 115 isolates of Salmonella enterica serovar Enteritidis from diarrhea patients in Incheon from Jan. 2004 to Dec. 2008. Overall, the highest rate of resistance was found to the following antimicrobial agents: nalidixic acid 54.8%, ampicillin 40.0%, ticarcillin 38.3%, chloramphenicol 20.9%, and tetracycline 17.4%. 91% (104 isolates) were resistant to one or more antimicrobial agents tested. Among the described antimicrobial resistant patterns, the predominant patterns were only nalidixic acid (44.3%), ampicillin-chloramphenicol-ticarcillin (11.3%,) and ampicillin-ticarcillin (10.4%). Overall, the resistant rate to one drug was 51.9%, two drugs 18.2%, three drugs 14.5%, four drugs 5.8%, and five or more drugs 9.7%.

인천연안 해역의 Vibrio속 세균분포 및 증식특성

황경화(Kyoung-Wha Hwang),공용우(Young-Woo Gong),이제만(Jae-Mann Lee),고종명(Jong-Myoung Go),김용희(Yong-Hee Kim),Bo-Young Oh 대한환경위생공학회 2008 대한환경위생공학회지 Vol.23 No.3

This study was conducted to investigate the effects of environmental factor such as temperature, salinity, turbidity, pH and dissolved oxygen on the growth of Vibrio spp.. In this survey, total 56 seawater samples were obtained from 8 different sites of the Incheon coastal area during the periods from april 2008 to october 2008. Enumeration of Vibrio spp. was determined by using the most probable number(MPN) procedure. Isolation rates of V. parahaemolyticus, V. vulnificus, V. cholerae in all samples tested were 44.0%, 21.4% and 13.1%, respectively. The enumeration of Vibrio spp. was very low correlated with water temperature and pH and negatively correlated with salinity, dissolved oxygen and turbidity. We found salinity to be the parameter most highly correlated with the enumeration of Vibrio spp. The highest rate of antibiotic resistance of V. vulnificus and V.parahaemolyticus was Cefazolin(11.5%), Ampicillin(70.8%), respectively.

인천지역 해양환경에서 분리된 비브리오패혈증군의 분포 및 항생제 내성 양성

황경화(Kyoung-Wha Hwang),오보영(Bo-Young Oh),공용우(Young-Woo Gong),이제만(Jae-Mann Lee),고종명(Jong-Myoung Go),김용희(Yong-Hee Kim) 대한환경위생공학회 2007 대한환경위생공학회지 Vol.22 No.4

We investigated the distribution and drug susceptibility test of V. vulnificus isolated from environmental sources in Incheon. In this survey, total 4,302 samples were obtained from different sites of the Incheon coastal area during the periods from march 2004 to November 2006. Among the 4,302 samples, 310 strains were isolated. The isolation rates of Vibrio vulnificus from fish, shellfish, estuarine water and sediment were 6.7%, 4.7%, 12.4% and 23.2%, respectively. The highest isolation rate was 14.6% in September and in spite of low temperature the isolation rate was 5.1% in November. The highest rate of antibiotic resistance was observed against streptomycin(15.3%), oefazclin(8.5%), oephalcthin(8.3%), amikacin(8.3%), oefoxitin(6.7%) and nalidixic acid(6.7%). Seventeen peroent of isolates were observed to be resistant to two or more of the antibiotics tested.

수원시 거주 기초생활수급자들의 노인 질병양상 연구

황경화(Kyoung Wha Hwang),문옥륜(Ok Ryun Moon) 한국노년학회 2003 한국노년학 Vol.23 No.1

This study was analyzed general characteristics and disease patterns of Beneficiary of National basic livelihood to the elderly who were really poor. This study was utilized data from the Health Interview Survey for the Recipients of Assistance in Suwon. The finding of the study are as follow: The prevalences of chronic diseases were 75.9%, and 78.8%, men and women, respectively. Those who had the prevalence of comorbidities more than two categories of disease were 51.7%. Regardless of the sex, the prevalences of musculoskeletal disease such as arthritis and cardiovascular disease such as hypertension were the highest. The obesity prevalence was 26.4% and 44.8%, men and women, respectively. There was a remarkable difference in the prevalence of hypertension between the health interview survey and health examination survey (male: 25.3% female: 36.0% vs male: 49.1%, female: 45.1%). This indicates that 24% of male and 9% of female did not recognize their prevalence status. 본 연구는 노인 인구 중에서도 노화와 빈곤이라는 중복장애를 가지고 있는 65세 이상 기초생활수급자들의 인구 사회학적 특성과 질병양상을 분석하는데 있다. 분석자료는 자산조사가 실제로 시행된 이후의 수원시에 거주하는 65세 이상 기초생활수급자 건강면접조사자료를 이용하였다. 연구결과 남성 75.9%, 여성 78.8%가 만성질환을 갖고 있는 것으로 조사되었고, 2가지 이상의 만성질환을 갖고 있는 복합이환상태는 51.7%였다. 성별과 무관하게 관절염등 근골격계질환과 고혈압등 심혈관계질환의 유병률이 높았다. 대상자들의 비만 유병율은 남성 26.4%, 여성 44.8%로 나타났다. 대표적인 순환기계 질환이면서 다른 질환의 위험요인인 고혈압 유병율은 건강면접조사에서는 성별간 유의하게 차이가 났으나(남성 25.3%, 여성 36.0%), 건강검진에서는 유의한 차이가 나지 않았다(남성 49.1%, 여성 45.1%). 그리고 조사대상자 남성의 24%, 여성의 9%가 자신이 고혈압에 노출되어 있다는 것을 모르고 있었다.

일반인 및 닭도축장 근무자에서 분리한 대장균의 항생제 내성 양상

황경화 ( Kyoung Wha Hwang ),김혜영 ( Hye Young Kim ),이미연 ( Mi Yeon Lee ),고연자 ( Yeon Ja Koh ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1

We monitored antibiotic resistance of Escherichia coli isolates from healthy volunteers of community and chicken butchers from February to July in 2006. From disc diffusion test on 473 E coli isolates from healthy volunteers of the community, the resistance rates of tetracycline, ampicillin, and ticarcillin were 44.8%, 40.8%, and 37.4%, respectively. In the disc diffusion test on 18 E coli isolates from chicken butchers , the resistance rates of tetracycline, nalidixic, streptomycin, and ampicillin were 94.4%, 61.1%, 55.6%, and 50.0%, respectively.

인천지역에서 분리된 비브리오 패혈증균의 특성

오보영,김정희,공용우,제갈승,김혜영,이미연,황경화,고연자,이제만,고종명,김용희,Oh, Bo-Young,Kim, Jung-Hee,Gong, Young-Woo,JeGal, Seung,Kim, Hye-Yeung,Lee, Mi-Yeon,Hwang, Kyoung-Wha,Koh, Yeon-Ja,Lee, Jae-Mann,Go, Jong-Myoung,Kim, Yong-H 한국미생물학회 2007 미생물학회지 Vol.43 No.4

2006년도 인천지역 해양환경에서 분리된 Vibrio vulnificus에 대한 생화학적, 분자생물학적 특성 및 항생제 감수성 결과를 알아보았다. 본 실험에 사용된 균주는 총 233주로 해수, 갯벌, 어패류, 수족관수에서 분리되었다. API 20E kit 실험 결과 15개 profile로 분류되었으며, 모든 균주가 ONPG와 Amygdalin 양성이었다. 209주를 대상으로 vvhA와 viuB 유전자 부위에 대해 PCR 실험결과 vvhA는 206주(98.6%) 양성, viuB는 110주(52.6%)가 양성이었으며, 특히 viuB 유전자부위에 대한 PCR 결과는 해수, 어패류, 갯벌에서 분리한 균주의 48%, 48.5%, 61.1%가 양성인 것으로 나타났다. 시험균주 175주에 대해 항생제 감수성 실험 결과 Imipenem (100.0%), Sulfamethoxazole/trimethoprim (98.9%), Tetracycline, Ciprofloxacin (98.3%), Ampicillin/sulbactam (97.1%), Chloramphenicol (96.6%), Cefepime (94.9%), Ceftriaxone (94.8%)이 감수성을 나타내었고, 항생제 하나 또는 그 이상의 약제에 대해 내성을 나타낸 것은 56주(32%)로 해수 28주(31.5%), 갯벌 21주(34.4%), 어패류 7주(29.2%)였다. V. vulnificus 233주에 대해 PFGE를 실시하여 dendrogram으로 분석한 결과 90%이상의 상동성 기준으로 126개의 유형으로 분리되었고, 58%이상의 상동성을 기준으로 13개의 cluster로 분류되었다. Cluster I는 104주(44.6%)로 가장 많은 균주들이 분포하였고 채취시기 대부분 I에 가장 많은 균주들이 분포하였으나 10월과 6월에 채취한 검체는 J에 16주(69.6%)와 13주(36.1%)로 가장 많은 균주들이 분포하였다. 7월에 채취한 검체에서 분리된 균주는 9개의 cluster에 속하였고 8월은 8개, 6월은 7개, 9월은 6개, 10월은 5개, 5월은 3개, 3월은 1개를 나타냈다. We performed the biochemical characteristics, molecular epidemiologocal analysis, and drug susceptibility test on V. vulnificus isolated from environmental sources in Incheon. For this study, 233 strains were isolated from seawater, sediment, shellfish. V. vulnificus isolates were divided into 15 biochemical groups, which were positive for ONPG and Amygdalin test. Among the 209 strains, 206 (98.6%) strains and 110 (52.6%) strains revealed positive for vvhA and viuB gene, and the viuB gene detection rates of V. vulnificus from seawater, shellfish and sediment were 48%, 48.5% and 61.6%, respectively. From disc diffusion test on 175 isolates, most of strains were sensitive to Imipenem (100.0%), Sulfamethoxazole/trimethoprim (98.9%), Tetracycline, Ciprofloxacin (98.3%), Ampicillin/sulbactam (97.1%), Ohloramphenicol (96.6%), Cefepime (94.9%) and Ceftriaxone (94.8%), multi-drug resistance rates was 31.5% of seawater, 34.4% of sediment and 29.2% of shellfish. PFGE was performed on 233 V. vulnificus isolates with the objective of investigating the extent of genetic diversity of these isolates in our region. We could find that at least 126 different PFGE patterns were generated according by 90% of similarity and 13 clusters by 58% of similarity. The major cluster was type I (44.6%) during the most of the year, and type J was frequent pattern in June and October. There were 9 distinct PFGE types in July, 8 types in August, 7 types in June, 6 types in September, 5 types in October 3 types in May and 1 type in March.