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윤보현,김영훈,Nguyen Bao Hung,오경환,김원일,함현휘,김현주,류경열,김세리 한국응용생명화학회 2018 Applied Biological Chemistry (Appl Biol Chem) Vol.61 No.5
To ensure the safety of Kimchi, the safety of Napa cabbage is the most important. Contaminated irrigation water can be a major cause of pathogens during growth of Napa cabbage. The purpose of this study was to investigate the microbial quality of irrigation water used in the cultivation of Napa cabbage. A total of 111 samples including surface water (n = 75) and groundwater (n = 36) collected from four different regions in Korea where Napa cabbage is intensively cultivated were analyzed for a fecal indicator (Escherichia coli) Moreover, 164 E. coli isolates from irrigation water were investigated for pathogenic characteristics including antibiotic resistance, pathogenic genes, serotype, and toxicity using Caenorhabditis elegans. E. coli was detected in 96% of surface water samples and 25% of groundwater samples. The level of E. coli in surface water (0.2–3.2 log MPN/100 mL) was higher than that in groundwater (0–2.0 log MPN/100 mL). When the 164 E. coli isolates were investigated concerning antibiotic resistance, resistance rates were 11.0%, 2.4%, 3.0%, 1.8%, 2.4%, 4.3%, and 3.0% for ampicillin, ampicillin/sulbactam, cefazolin, cefoxitin, gentamicin, levofloxacin, and trimethoprim/sulfamethoxazole, respectively. In addition, 10 (6.1%) of the isolates were positive for the eaeA gene, indicative of enteropathogenic E. coli. Eight of these 10 isolates were obtained from the surface water of the mountainous region II and were toxic to C. elegans. The results indicate the need to manage the microbial risk of irrigation water to enhance the safety of cultivated Napa cabbage.
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임종국,김기영,모창연,오경민,김건섭,유현채,함현휘,김영태,김성민,김문수 한국농업기계학회 2017 바이오시스템공학 Vol.42 No.4
Purpose: This study was conducted to discriminate between normal hulled barley and Fusarium (Fusarium asiaticum and Fusarium graminearum) infected hulled barley by using the near-infrared spectroscopy (NIRS) technique. Methods: Fusarium asiaticum and Fusarium graminearum were artificially inoculated in hulled barley and the reflectance spectrum of the barley spike was obtained by using a near-infrared spectral sensor with wavelength band in the range 1,175-2,170 nm. After obtaining the spectrum of the specimen, the hulled barley was cultivated in a greenhouse and visually inspected for infections. Results: From a partial least squares discriminant analysis (PLS-DA) prediction model developed from the raw spectrum data of the hulled barley, the discrimination accuracy for the normal and infected hulled barley was 99.82% (563/564) and 100% (672/672), respectively. Conclusions: NIRS is effective as a quick and nondestructive method to detect whether hulled barley has been infected with Fusarium. Further, it expected that NIRS will be able to detect Fusarium infections in other grains as well.
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