Baicalein protects HT22 murine hippocampal neuronal cells against endoplasmic reticulum stress-induced apoptosis through inhibition of reactive oxygen species production and CHOP induction

최지현,A Young Choi,Hana Yoon,최원재,윤경식,하주헌,Eui-Ju Yeo,강인숙 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.12

Baicalein is one of the major flavonoids in Scutellaria baicalensis Georgi and possesses various effects, including cytoprotection and anti-inflammation. Because endoplasmic reticulum (ER) stress has been implicated in neurodegenerative diseases, such as Alzheimer’s disease, Parkinson’s disease, and cerebral ischemia, we investigated the effects of baicalein on apoptotic death of HT22 mouse hippocampal neuronal cells induced by thapsigargin (TG) and brefeldin A (BFA), two representative ER stress inducers. Apoptosis,reactive oxygen species (ROS) production, and mitochondrial membrane potential (MMP) were measured by flow cytometry. Expression level and phosphorylation status of ER stress-associated proteins and activation and cleavage of apoptosis-associated proteins were analyzed by Western blot. Baicalein reduced TG- and BFA-induced apoptosis of HT22 cells and activation and cleavage of apoptosis-associated proteins, such as caspase-12 and -3 and poly(ADP-ribose)polymerase. Baicalein also reduced the TG- and BFA-induced expression of ER stress-associated proteins,including C/EBP homologous protein (CHOP)and glucose-regulated protein 78, the cleavage of X-box binding protein-1 and activating transcription factor 6α, and the phosphorylation of eukaryotic initiation factor-2α and mitogen-activated protein kinases,such as p38, JNK, and ERK. Knock-down of CHOP expression by siRNA transfection and specific inhibitors of p38 (SB203580), JNK (SP600125), and ERK (PD98059) as well as anti-oxidant (N-acetylcysteine)reduced TG- or BFA-induced cell death. Baicalein also reduced TG- and BFA-induced ROS accumulation and MMP reduction. Taken together, these results suggest that baicalein could protect HT22 neuronal cells against ER stress-induced apoptosis by reducing CHOP induction as well as ROS accumulation and mitochondrial damage.

분쟁해결을 위한 대체적 수단으로서 ITLOS 권고적 의견 절차 활용 - SRFC 권고적 의견 사건(사건번호 21)을 중심으로 -

최지현,Choi, Jee-hyun 한국해양과학기술원 2022 Ocean and Polar Research Vol.44 No.2

SRFC (Sub-Regional Fisheries Commission) requested to the ITLOS (International Tribunal for the Law of the Sea) an advisory opinion relating to the IUU (Illegl, Unreported, and Unregulated) fishing (Case No-21 of the ITLOS). Since, in the UNCLOS, there is no article authorizing the jurisdiction of the ITLOS full court's Advisory opinion, so various scholarly opinion wad divided. But ITLOS delivered its Advisory opinion confirming its jurisdictional competence over the Advisory proceedings with its legal opinion about the IUU issues. It opens new possibility of the alternative dispute settlement mechanism of the ITLOS through the advisory procedures. In reality, there has been a view that ICJ (International Court of Justice) could take the part of a kind of dispute settlement through its Advisory procedures. But the advisory procedures of the ITLOS, with no definite clause in UNCLOS about the advisory procedures, which provides more allowances for the function of advisory opinion as the alternative dispute settlement mechanism. ITLOS accepted the requests of the advisory opinion by the State parties through international organization or themselves directly. And the advisory opinion of the ITLOS aims the interpretation and application into the special issues-specially IUU fishing in Case No. 21 of the ITLOS-. Those factors could enable more enhanced role of the ITLOS as an alternative dispute settlement mechanism. But those possibility has contain risk of excessive and unlimited advisory role of the ITLOS. So it is important to focus on the restriction on the role of the State parties in the request of the advisory opinion to the ITLOS. In this regard it is meaningful that the ITLOS has suggested a kind of legal standing in the advisory procedures in that only coastal States could request the Advisory opinion about the IUU in their EEZ. Furthermore the discretionary power of the ITLOS in the Article 138 of the Rules of the Tribunal could curtail the abuse of the Advisory opinion initiated by the States parties of the UNCLOS. Under this framework, Advisory opinion could broaden more alternative option to the disputes between State parties of the UNCLOS in that after being delivered detailed interpretation of the UNCLOS about the specific issues, States parties could devote themselves to searching for flexible solution for the disputes between State parties. It could obtain legal explanation about the dispute under the Article 297 and Article 298 by detouring the jurisdiction limits through advisory procedures.

주요 식품영양소의 수요분석

최지현,이계임 ( Ji Hyeon Choi & Kyei Im Lee ) 한국농업경제학회 1997 農業經濟硏究 Vol.38 No.1

소아 급성 위장관염의 중증도 평가를 위한 기존 중증도 점수척도들의 비교

최지현,정태웅,김성준,정주영,김민성,한승범,강진한,김상용,임정우,김황민,박재홍,조대선,마상혁,정혜숙,천두성,고대균,김종현,Choi, Jee-Hyun,Jung, Tae Woong,Kim, Seong Joon,Chung, Ju-Young,Kim, Min-Sung,Han, Seung Beom,Kang, Jin-Han,Kim, Sang Yong,Rhim, Jung 대한소아감염학회 2014 Pediatric Infection and Vaccine Vol.21 No.1

목적: 급성 위장관염으로 입원한 소아 환자들의 중증도를 여러 척도로 평가하여 각 척도간의 차이를 비교하고, 원인 바이러스가 밝혀진 예를 따로 분류하여 원인 병원체에 따른 임상양상 및 중증도의 차이를 알아보고자 하였다. 방법: 전향적으로 전국 8개 지역 9개 의료기관의 소아청소년과에 급성 위장관염으로 진단받아 입원한 5세 미만의 환자를 대상으로 Vesikari 척도, Clark 척도, 수정 Flores 척도로 중증도를 평가하였다. 연구군으로부터 수집된 분변 검체로 로타바이러스, 장 아데노바이러스는 효소면역법으로, 노로바이러스, 아스트로바이러스, 사포바이러스는 RT-PCR 방법으로 검출하였다. 결과: 최종 연구 대상군은 총 214례로 남녀비는 1.58:1이고, 연령층은 6개월 미만이 35명(16.4%), 6-23개월이 105명(49.1%), 24-59개월이 74명(34.5%)이었다. Vesikari 척도와 Clark 척도간 일치율은 0.521 (P<0.001)이고, '중증'인 경우가 Vesikari 척도는 60.7%, Clark 척도는 2.3%로 Clark 척도가 '중증' 평가에 더욱 엄격하였다. 결론: 급성 위장관염의 중증도를 평가하는 각 척도들간에는 차이가 있었다. 각 연구자들의 결과를 객관적으로 비교하기 위해서는 척도를 하나로 통일하거나 각 척도들의 평가기준을 모두 조사하여 각 척도의 중증도를 동시에 얻는 것이 필요하다. Purpose: This study aimed to evaluate the disease severity of children suffering from gastroenteritis using different scales. The results are compared and subsequently classified on the basis of the type of virus causing the disease in order to investigate the differences in clinical characteristics and disease severity according to pathogen. Method: This study was conducted prospectively with patients under 5 years of age diagnosed with acute gastroenteritis and hospitalized at 9 medical institutions in 8 regions across the Republic of Korea. Disease severity was evaluated using the Vesikari Scale, the Clark Scale, and the modified Flores Scale. Fecal samples collected from patients were used to detect rotavirus and enteric adenovirus by enzyme immunoassay, and for RT-PCR of norovirus, astrovirus, and sapovirus. Results: There were a total of 214 patients with a male : female ratio of 1.58 : 1, of which 35 were under the age of 6 months (16.4%), 105 were aged 6-23 months (49.1%), and 74 were aged 24-59 months (34.5%). The rate of concordance between the Vesikari and Clark Scales was 0.521 (P<0.001) and, in severe cases, the Vesikari Scale was 60.7% and Clark Scale was 2.3%, indicating that the Clark Scale was stricter in the evaluation of severe cases. Conclusions: In children with gastroenteritis, there were differences in disease severity based on the scale used. Therefore, to achieve consistent results among researchers, either only a single scale or a measure of all scales should be used to determine disease severity.

다면적 인성검사 II 재구성판으로 살펴본 주요우울장애 자살 시도자의 심리적 특성

최지현,박은희,Choi, Ji-Hyun,Park, Eun-Hee 한국정신신체의학회 2021 정신신체의학 Vol.29 No.1

연구목적 본 연구는 MMPI-2-RF를 통해 주요우울장애 환자군 중 자살 시도자들의 심리적 특성을 확인하고자 하였다. 방 법 본 연구는 한림대학교 성심병원 정신건강의학과를 방문한 입원 및 외래 환자 중 한국판 K-MINI-Plus 5.0을 통해 DSM-IV의 주요우울장애 진단적 준거를 충족시키는 자 107명을 대상으로 하였다. 이들을 C-SSRS를 사용하여 자살 시도군(n=43)과 비시도군(n=64)으로 분류하였고 ANOVA를 통해 두 집단 간 MMPI-2-RF 척도 점수에서의 차이를 조사하였다. 더불어 우울 증상의 심각도와 공존질환의 이환 여부가 자살 시도 여부에 영향을 주었을 가능성을 고려해 이를 공변인으로 하여 추가적으로 ANCOVA 분석을 수행하였다. 결 과 본 연구에서 자살 시도군이 비시도군에 비해 효능감 결여(NFC), 대인관계 수동성(IPP), 자살/죽음 사고(SUI) 척도에서 유의하게 높은 점수를 나타냈다(p<0.05). 다만 자살/죽음 사고(SUI) 척도의 경우 우울 증상과 공존질환의 이환 여부를 통제한 이후에는 두 집단 간 차이에서 통계적으로 유의한 경향성을 보여주었다(p<0.10). 결 론 본 연구의 결과를 통해 MMPI-2-RF가 중등도 이상의 증상 심각도를 지닌 주요우울장애 집단에서 실제 자살 시도로 이행될 위험이 있는 환자를 심리적 특성을 확인하는데 유용한 도구임을 확인하였다. 이러한 결과를 토대로 본 연구의 한계와 향후 연구 방향에 대해 논의하였다. Objectives : This study was conducted to investigate differences in psychological characteristics between major depressive disorder (MDD) patients with and without suicide attempt using MMPI-2-RF. Methods : Subjects were 107 MDD patients who had visited the department of psychiatry of hallym university hospital and met the DSM-IV diagnostic criteria of MDD by the korean version of MINI-Plus 5.0.0. The patients were divided into suicidal attempters (n=43) and non-suicidal attempters (n=64) using C-SSRS. The one-way ANOVA was used to compare MMPI-2-RF scale scores between two groups. Additionally, ANCOVA was conducted considering the severity of depressive symptom and comorbidity as covariate. Results : Our results showed that Suicide/Death Ideation (SUI), Inefficacy (NFC) and Interpersonal Passivity (IPP) scales were significantly higher in the MDD patients with suicidal attempt compared to MDD patients without suicidal attempt (p<0.05). However, after controlling for the severity of depressive symptom and comorbidity, SUI scale showed a significant tendency (p<0.10). Conclusions : The result suggests that MMPI-2-RF scales could be a useful tool for identifying patients transitioning to actual suicidal attempts in the moderate or severe major depressive disorder group. Limitations of this study and directions for further research are also discussed.

평균기온과 식생의 영향을 고려한 격자기반 일 지표토양온도 예측 모형 개발

최지현 ( Chi Hyun Choi ),최대규 ( Dae Gyu Choi ),최현일 ( Hyun Il Choi ),김경헌 ( Kyung Hyun Kim ),김상단 ( Sang Dan Kim ) 한국물환경학회 2012 한국물환경학회지 Vol.28 No.1

Land surface temperature in ecohydrology is a variable that links surface structure to soil processes and yet its spatial prediction across landscapes with variable surface structure is poorly understood. And there are an insufficient number of soil temperature monitoring stations. In this study, a grid-based land surface temperature prediction model is proposed. Target sites are Andong and Namgang dam region. The proposed model is run in the following way. At first, geo-referenced site specific air temperatures are estimated using a kriging technique from data collected from 60 point weather stations. Then surface soil temperature is computed from the estimated geo-referenced site-specific air temperature and normalized difference vegetation index. After the model is calibrated with data collected from observed remote-sensed soil temperature, a soil temperature map is prepared based on the predictions of the model for each geo-referenced site. The daily and monthly simulated soil temperature shows that the proposed model is useful for reproducing observed soil temperature. Soil temperatures at 30 and 50 cm of soil depth are also well simulated.

농어업 부가가치의 새로운 창출을 위한 식품산업의 중장기 발전 전략(3/5차연도)

최지현(Ji-Hyeon Choi),전창곤(Chang-Gon Jeon),이용선(Yong-Sun Lee),장재봉(Jae Bong Chang),조소현(So-Hyun Cho),하홍근,박규은,전혜미,박재홍,강혜정 한국농촌경제연구원 2011 한국농촌경제연구원 기본연구보고서 Vol.- No.-

중학교 디자인 수업의 흥미도, 이해도, 만족도 평가 연구

최지현(Ji Hyun Choi),장광집(Kwang Chib Chang) 경희대학교 산학협력단 예술디자인연구원 2014 예술· 디자인학연구 Vol.17 No.1

일부 남성 소방직공무원의 수면의 질 및 그의 관련요인

최지현(Jee-Hyeon Choi),김광환(Kwang-Hwan Kim),조영채(Young-Chae Cho) 한국산학기술학회 2018 한국산학기술학회논문지 Vol.19 No.5

본 연구는 소방직공무원들의 수면의 질을 평가하고, 이들의 일반적 특성, 건강습관특성 및 직업적 특성과 수면의 질과의 관련성을 규명하고자 시도하였다. 조사는 2016년 10월에 D광역시의 5개 소방서에 근무하고 있는 남성 소방직공무원 600명을 대상으로 Pittsburgh대학에서 개발한 수면의 질에 관한 질문 표(PSQI)를 사용한 자기기입식 설문조사에 의하였다. 연구결과, 전체 조사대상자의 13.2%는 「수면의 질이 좋은 군」으로, 86.8%는 「수면의 질이 좋지 않은 군」으로 나타났다. 일반적 특성 및 건강습관 특성별 수면의 질은 주관적인 건강상태가 좋지 않을수록, 흡연군보다 비흡연군에서, 주관적인 수면의 질이 높다는 군보다 낮다는 군에서 「수면의 질이 좋지 않다는 군」의 비율이 유의하게 높았다. 직업적 특성별 수면의 질은 다른 업무보다 화재진압을 한다는 군에서, 비교대근무군보다 교대근무군에서, 업무의 신체적 부담이 낮은 군보다 높은군에서, 업무에 대한 만족도가 높은 군보다 낮은 군에서, 업무에 대한 적성도가 높은 군보다 낮은 군에서, 현재의 소방직에 대한 직업전환의사가 없다는 군보다 있다는 군에서 「수면의 질이 좋지 않다는 군」의 비율이 유의하게 높았다. 다중회귀분석결과, 수면의 질에 영향을 미치는 요인으로는 주관적 건강상태, 음주상태, 주관적인 수면의 질, 근무경력, 직위, 업무의 신체적 부담정도가 유의한 변수로 선정되었으며 이들의 설명력은 24.4%이었다. 위와 같은 연구결과는 소방직공무원들의 수면의 질은 일반적 특성, 건강습관 특성 및 직업적 특성의 여러 요인들과 유의한 관련성이 있음을 시사하고 있다. This study was conducted to determine the association between quality of sleep (QOL) and the general characteristics, the health-behavioral factors, and the occupational characteristics of firefighting officers. The survey was conducted in October 2016 with a self-administered questionnaire using the Pittsburgh Sleep Quality Index (PSQI) developed by Pittsburgh University, targeting 600 male firefighting officers working at five fire stations in D city. As a result, the QOL of the study subjects measured by the PSQI revealed 13.2% to be good, and 86.8% to be poor. In relation to general characteristics and health habits, the rate of the group who had poor quality sleep was significantly higher in those with a lower subjective health status, in the non-smoking group, and in the low sleep-quality group, compared to the good sleep-quality group. For occupational characteristics, the rate of those with poor quality sleep was significantly higher in groups such as the fire suppression group, the shift work group, the hard physical labor group, the group with a lower sense of satisfaction in their work, and in the group classed as unfit for the job but who were not considering quitting their jobs, compared to their respective counterparts. Multiple regression analysis revealed factors of influence with explanatory powers of 24.4% for subjective health status, alcohol use, subjective quality of sleep, career, job position, and physical labor. The results of this study suggest that the quality of sleep for firefighting officers is significantly related to general characteristics, health-behavioral characteristics, and occupational characteristics.

차량 노면 진동을 이용한 공진형 선형 발전기 시스템의 강건최적설계

최지현(Ji Hyun Choi),김진호(Jin Ho Kim),박상신(Sang-Shin Park),서태원(TaeWon Seo) 한국생산제조학회 2014 한국생산제조학회지 Vol.23 No.4