LED용Mg²⁺·Ba²⁺Co-Doped Sr₂SiO₄:Eu 노란색 형광체의 발광특성

최경재,지순덕,김창해,이상혁,김호건,Choi, Kyoung-Jae,Jee, Soon-Duk,Kim, Chang-Hae,Lee, Sang-Hyuk,Kim, Ho-Kun 한국세라믹학회 2007 한국세라믹학회지 Vol.44 No.3

An improvement for the efficiency of the $Sr_{2}SiO_{4}:Eu$ yellow phosphor under the $450{\sim}470\;nm$ excitation range have been achieved by adding the co-doping element ($Mg^{2+}\;and\;Ba^{2+}$) in the host. White LEDs were fabricated through an integration of an blue (InGaN) chip (${\lambda}_{cm}=450\;nm$) and a blend of two phosphors ($Mg^{2+},\;Ba^{2+}\;co-doped\;Sr_{2}SiO_{4}:Eu$ yellow phosphor+CaS:Eu red phosphor) in a single package. The InGaN-based two phosphor blends ($Mg^{2+},\;Ba^{2+}\;co-doped\;Sr_{2}SiO_{4}:Eu$ yellow phosphor+CaS:Eu red phosphor) LEDs showed three bands at 450 nm, 550 nm and 640 nm, respectively. The 450 nm emission band was due to a radiative recombination from an InGaN active layer. This 450 nm emission was used as an optical transition of the $Mg^{2+},\;Ba^{2+}\;co-doped\;Sr_{2}SiO_{4}:Eu$ yellow phosphor+CaS:Eu red phosphor. As a consequence of a preparation of white LEDs using the $Mg^{2+},\;Ba^{2+}\;co-doped\;Sr_{2}SiO_{4}:Eu$ yellow phosphor+CaS:Eu red phosphor yellow phosphor and CaS:Eu red phosphor, the highest luminescence efficiency was obtained at the 0.03 mol $Ba^{2+}$ concentration. At this time, the white LEDs showed the CCT (5300 K), CRI (89.9) and luminous efficacy (17.34 lm/W).

자외선 여기용 청색 및 황색 형광체의 발광특성

최경재,박정규,김경남,김창해,김호건,Choi, Kyoung-Jae,Park, Joung-Kyu,Kim, Kyung-Nam,Kim, Chang-Hae,Kim, Ho-Kun 한국세라믹학회 2006 한국세라믹학회지 Vol.43 No.5

We have synthesized a $Eu^{2+}-activated\;Sr_3MgSi_2O_8$ blue phosphor and $(Sr,Ba)_2SiO_4$ yellow phosphor and prepared white LEDs by combining these phosphors with a InGaN UV LED chip. Three distinct emission bands from the InGaN-based LED and the two phosphors are clearly observed at 405 nm, 460 nm and at around 560 nm, respectively. The 405 nm emission band is due to a radiative recombination from a InGaN active layer. This blue emission was used as an optical transition of the $Sr_3MgSi_2O_8:Eu$ blue phosphor and $(Sr,Ba)_2SiO_4:Eu$ yellow phosphor. The 460 nm and 560 nm emission band is ascribed to a radiative recombination of $Eu^{2+}$ impurity ions in the $Sr_3MgSi_2O_8:Eu$ and $(Sr,Ba)_2SiO_4$ host matrix. As a consequence of a preparation of UV White LED lamp using the $Sr_3MgSi_2O_8:Eu$ blue phosphor and $(Sr,Ba)_2SiO_4:Eu$ yellow phosphor, the highest luminescence efficiency was obtained at the ration of epoxy/two phosphor (1/0.2361). At this time, the CIE chromaticity was CIE x = 0.3140, CIE y = 0.3201 and CCT (6500 K).

LED용 Ba²⁺ Co-Doped Sr₂SiO₄:Eu 황색 형광체의 발광특성

최경재,박정규,김경남,김창해,김호건,Choi, Kyoung-Jae,Park, Joung-Kyu,Kim, Kyung-Nam,Kim, Chang-Hae,Kim, Ho-Kun 한국세라믹학회 2006 한국세라믹학회지 Vol.43 No.3

We have synthesized a $Eu^{2+}-activated\;{(Sr,Ba)}_2SiO_4$ yellow phosphor and investigated the development of blue LEDs by combining the phosphor with a InGaN blue LED chip (${\lambda}_{em}$=405 nm). The InGaN-based ${(Sr,Ba)}_2SiO_{4}:Eu$ LED lamp shows two bands at 405 nm and 550 nm. The 405 nm emission band is due to a radiative recombination from a InGaN active layer. This 405 nm emission was used as an optical transition of the ${(Sr,Ba)}_2SiO_{4}:Eu$ phosphor. The 550 nm emission band is ascribed to a radiative recombination of $Eu^{2+}$ impurity ions in the ${(Sr,Ba)}_2SiO_4$ host matrix. In the preparation of UV Yellow LED Lamp with ${(Sr,Ba)}_2SiO_{4}:Eu$ yellow phosphor, the highest luminescence efficiency was obtained at the epoxy-to-yellow phosphor ratio of 1:0.45. At this ratio, the CIE chromaticity was x=0.4097 and y=0.5488.

LNG 외조 저장탱크의 구조적 특성을 고려한 상태평가 기준 개발

최경재(Kyoung-Jae Choi),서창주(Chang-Joo Seo),김영구(Young-Gu Kim),조영도(Young-Do Jo),김정훈(Jung-Hoon Kim) 한국가스학회 2017 한국가스학회지 Vol.21 No.5

한국은 세계 2위의 LNG 수입국이고 2017년 기준 약 70기의 LNG 저장탱크가 상업운전 중에 있다. LNG산업의 핵심설비인 LNG 저장탱크는 1986년 상업운전 이후 설계보증 연한인 30년이 경과 되면서 도시가스사업법 개정을 통해 2014년부터 정밀안전진단을 수행하고 있다. 정밀안전진단 시 LNG 저장탱크의 외조 콘크리트에 대해서 상태평가를 할 수 있는 기준이 부재한 상태이다. LNG 저장탱크의 구조적 특성과 기존 토목구조물 상태평가 기준 분석을 통해 저장탱크의 대표부재에 대한 상태평가기준을 개발하였다. 개발된 객관적 상태평가기준은 LNG 저장탱크의 안전성 및 신뢰성을 향상시키고 보수기준을 제시할 수 있다. South Korea is the world`s second-largest importer of LNG and possess about 70 tanks which are in operation by 2017. Thirty years as the design warranty period have exceeded since LNG storage tanks as the core facility of LNG industry were constructed in 1986. The LNG storage tank is under precision safety diagnosis from 2014 due to urban gas business act amendment. There is no criteria of condition evaluation for outer tank of LNG storage tank at the time of precision safety diagnosis. Through analysis of structural characteristic of LNG storage tank and civil structure condition evaluation standards, the criteria of condition evaluation for main members was developed. The criteria of objective condition evaluation can improve safety and reliability of LNG storage tank and suggest matenance criteria.

Haemophilus influenzae의 Acetohydroxyacid Synthase Catalytic Subunit 재조합 단백질 발현 및 특성

노경미,최경재,박준식,윤문영,Noh, Kyoung-Mi,Choi, Kyoung-Jae,Park, Joon-Shik,Yoon, Moon-Young 한국미생물학회 2007 미생물학회지 Vol.43 No.1

Acetohydroxylacid synthase (E.C.2.2.1.6.,AHAS)는 박테리아, 곰팡이, 식물 등에서 필수 아미노산중 세 가지 아미노산(Val, Leu, Ile)의 생합성에 관여하는 효소중 하나이다. Haemophilus influenzae에 대한 AHAS의 효소특성을 규명하기 위하여 H. influenzae의 AHAS catalytic subunit 유전자(TIGR access code HI2585)를 pET28a 발현 벡터에 삽입시켰고, 대장균 BL21(DE3)에서 C-말단에 일련의 histidine을 갖는 재조합 단백질로 발현시켰고, Histidine-tag affinity chromatography 및 gel filtration chromatography를 이용하여 단일 단백질로 정제하였다. 정제하여 얻은 단백질은 최대 15 mg/ml까지 농축이 가능하였다. 정제된 단백질의 분자량은 SDS-PAGE 전기 영동법을 이용하여 약 63.9 kDa의 분자량을 확인하였다. AHAS 효소 활성은 discontinuous colorimetric assay방법을 이용하여 측정하였다. H. influenzae AHAS catalytic subunit의 specific activity는 3.22 U/mg 이었다. 또한AHAS의 최적 활성 온도와 pH는 각각$37^{\circ}C$와 pH 7.5이었다. AHAS 효소 활성은buffer의 종류에 따라 차이가 있었으며, 유기용매가 증가함에 따라 효소 활성도 감소하였다. Acetohydroxyacid synthase (E.C.2.2.1.6., AHAS) is the enzyme that catalyses the first step in the synthesis of the branched-chain amino acids valine, leucine and isoleucine. The AHAS gene (TIGR access code HI2585) from Heamophilus influenzae was cloned into the bacterial expression vector pET-28a and expressed in the Escherichia coli strain BL21(DE3). The expressed enzyme was purified by $Ni^{2+}-charged$ HiTrap chelating HP column. The purified enzyme appears as a single band on SDS-PAGE with a molecular mass of about 63.9 kDa. The enzyme exhibits absolute dependence on the three cofactors FAD, $MgCl_{2}$ and thiamine diphosphate for activity. Specific activity of purified enzyme has 3.22 unit/mg and optimum activity in the pH 7.5 at $37^{\circ}C$. This enzyme activity has an effect on the buffer. When comparing the enzyme activity against the organic solvent, it followed in type and the difference it is but even from the aqueous solution where the organic solvent is included with the fact that the enzyme activity is maintained.

Yeast내에서 탄저병 원인균인 Bacillus anthracis의 치사독소인 Lethal Factor 단백질 발현

황혜현,김정목,최경재,정회일,한성환,구본성,윤문영,Hwang Hyehyun,Kim Joungmok,Choi Kyoung-Jae,Chung Hoeil,Han Sung-Hwan,Koo Bon-Sung,Yoon Moon-Young 한국미생물학회 2005 미생물학회지 Vol.41 No.4

Bacillus anthracis는 탄저병의 병원체이다. 탄저병의 독소는 Bacillus anthracis가 가진 세가지 독소로 이루어져 있다. protective antigen (PA), lethal factor (LF)그리고 edema factor (EF)로 구성되어 있다. PA는 세포수용체와 결합하여 활성화 과정을 거친 후 LF 흑은 EF를 세포질 안으로 이동시켜 주는 역할을 한다. LF는 금속이온 $(Zn^{2+})$ 의존적 단백질 가수분해 효소로써 탄저병에 감염된 동물들의 치사독소로 작용하게 된다. 따라서 LF에 대한 특성 분석 및 억제재 개발에 관한 연구는 탄저치료제 개발에 매우 중요한 과정이라 할 수 있다. 본 연구에서는 탄저독소의 치료제 개발을 위해 선행되어야 하는 LF 고처리량 활성검증방법 및 저해제 선별에 더 높은 효율을 가지기 위해 이러한 시스템 방법 등을 이용하여 세포내 검정방법의 기초 자료를 마련하고자 하였다. 이를 위하여 yeast를 숙주로 한 LF 발현 vector의 구축과, 구축한 발현 시스템을 yeast에 형질전환 하여 plasmid의 안정성 및 LF유전자의 발현을 확인하였다. 본 연구는 LF유전자의 발현을 진핵세포 내에서 처음으로 시도했으며, 세포내 검증 시스템 도입의 기초적 자료를 제공하였다. Yeast내에서의 LF의 발현은 탄저병의 저해제 선별이나 활성측정검증을 생체 내에서 용이하게 할 수 있다는 가능성을 나타냈다. Anthrax is an infectious disease caused by the gram-positive bacterium, Bacillus anthracis. Anthrax toxin is a tripartite toxin comprising of protective antigen (PA), lethal factor (LF) and edema factor (EF). PA is the receptor-binding component, which facilitates the entry of LF or EF onto the cytosol. LF is a zinc-dependent metalloprotease, which is a critical virulence factor in cytotoxicity of infected animals. Therefore, it is of interest to develop its potent inhibitors for the neutralization of anthrax toxin. The first step to identify the inhibitors is the development of a rapid, sensitive, and simple assay method with a high-throughput ability. Much efforts have been concentrated on the preparation of powerful assays and on the screening of inhibitors using these system. In the present study, we have tried to construct anthrax lethal factor in yeast expression system to prepare cell-based high-throughput assay system. Here, we have shown the results covering the construction of a new vector system, subcloning of LF gene, and the expression of target gene. Our results are first trial to express LF gene in eukaryote and provide the basic steps in design of cell-based assay system.

백색 LED용 청록색 BaSi₂O₂N₂:Eu²⁺ 형광체의 합성 및 응용

지순덕,최강식,최경재,김창해,Jee, Soon-Duk,Choi, Kang-Sik,Choi, Kyoung-Jae,Kim, Chang-Hae 한국재료학회 2011 한국재료학회지 Vol.21 No.5

We have synthesized bluish-green, highly-efficient $BaSi_2O_2N_2:Eu^{2+}$ and $(Ba,Sr)Si_2O_2N_2:Eu^{2+}$ phosphors through a conventional solid state reaction method using metal carbonate, $Si_3N_4$, and $Eu_2O_3$ as raw materials. The X-ray diffraction (XRD) pattern of these phosphors revealed that a $BaSi_2O_2N_2$ single phase was obtained. The excitation and emission spectra showed typical broadband excitation and emission resulting from the 5d to 4f transition of $Eu^{2+}$. These phosphors absorb blue light at around 450 nm and emit bluish-green luminescence, with a peak wavelength at around 495 nm. From the results of an experiment involving Eu concentration quenching, the relative PL intensity was reduced dramatically for Eu = 0.033. A small substitution of Sr in place of Ba increased the relative emission intensity of the phosphor. We prepared several white LEDs through a combination of $BaSi_2O_2N_2:Eu^{2+}$, YAG:$Ce^{3+}$, and silicone resin with a blue InGaN-based LED. In the case of only the YAG:$Ce^{3+}$-converted LED, the color rendering index was 73.4 and the efficiency was 127 lm/W. In contrast, in the YAG:$Ce^{3+}$ and $BaSi_2O_2N_2:Eu^{2+}$-converted LED, two distinct emission bands from InGaN (450 nm) and the two phosphors (475-750 nm) are observed, and combine to give a spectrum that appears white to the naked eye. The range of the color rendering index and the efficiency were 79.7-81.2 and 117-128 lm/W, respectively. The increased values of the color rendering index indicate that the two phosphor-converted LEDs have improved bluish-green emission compared to the YAG:Ce-converted LED. As such, the $BaSi_2O_2N_2:Eu^{2+}$ phosphor is applicable to white high-rendered LEDs for solid state lighting.

Yeast내에서 MEK1 융합 단백질 발현 및 Lethal Factor 활성 검증

황혜현,김정목,최경재,박해철,한성환,정회일,구본성,박준식,윤문영,Hwang, Hye-Hyun,Kim, Joung-Mok,Choi, Kyoung-Jae,Park, Hae-Chul,Han, Sung-Hwan,Chung, Hoe-Il,Koo, Bon-Sung,Park, Joon-Shik,Yoon, Moon-Young 한국미생물학회 2006 미생물학회지 Vol.42 No.3

Anthrax lethal toxin은 탄저병의 치사원인이 되는 독소이며, Lethal toxin은 두 종류의 단백질 PA (Protective antigen)과 LF (lethal factor)로 구성되어 진다. PA는 세포표면의 수용체와 결합하여 LF를 세포질 안으로 이동시켜 주는 역할을 한다. LF는 금속 이온$(Zn^{2+})$ 의존적 단백질 가수분해 효소로써 MKKs[MAPK (mitogen-activated protein kinase) kinases] 집단 단백질의 아미노 말단 부분을 절단하여 대상 세포를 죽음으로 유도하는 것으로 알려져 있다. 본 연구에서는 LF에 대한 특성 분석 및 억제제 개발에 과한 연구를 위해 cell-based high-throughtput screens 개발에 선행되어야 하는 기초 자료를 마련하는데 그 목적이 있다. 이를 위하여 LF의 절단 대상이 되는 기질이 MEK1을 yeast내에서 동시 발현시켜 LF의 활성을 검증하였다. 먼저 효모(Saccharomyces cerevisiae)를 숙주로 하여 LF의 기질인 MEK1 발현 vector를 구축하였고, 구축된 발현 system을 기본을 LF 활성을 검증하고자 yeast에 형질전환하여 plasmid의 안전성 및 MEK1 유전자의 발현 및 LF에 의한 MEK1 아미노말단의 절단 부위를 확인하였다. 본 연구는 세포내 검증 system 도입의 기초적 자료를 제공하였으며, yeast내의 MEK1 발현은 탄저병의 저해제 선별 및 활성 측정 검증을 생체에서 고효율적이며, 안정적으로 할 수 있다는 가능성을 나타냈다. Lethal toxin is a critical virulence factor of anthrax. It is composed two protein: protective antigen (PA) and lethal factor (LF). PA binds to specific cell surface receptors and, forms a membrane channel that mediates entry of LF into the cell. LF is a zinc-dependent metalloprotease, which cleaves MKKs [MAPK (mitogen-activated protein kinase) kinases] at peptide bonds very close to their N-termini. In this study, we suggest application of cell-based assays in the early phase of drug discovery, with a particular focus on the use of yeast cells. We constructed MEK1 expression system in yeast to determine LF activity and approached cell-based assay system to screen inhibitors, in which the results covering the construction of LF-substrate in yeast expression vector, expression, and LF-mediated proteolysis of substrate were described. These results could provided the basic steps in design of cell-based assay system with the high efficiency, rapidly and easy way to screening of inhibitors.

LED 기반 백색 조명의 색온도 및 연색지수에 따른 감성 평가

지순덕 ( Soon Duk Jee ),최경재 ( Kyoung Jae Choi ),김호건 ( Ho Kun Kim ),이상혁 ( Sang Hyuk Lee ) 한국감성과학회 2006 감성과학 Vol.9 No.4

백색 LED 조명의 색온도에 관한 감성평가

지순덕(Soon-Duk Jee),이상혁(Sang-Hyuk Lee),최경재(Kyoung-Jae Choi),박정규(Joung-Kyu Park),김창해(Chang-Hae Kim) 한국조명·전기설비학회 2008 조명·전기설비학회논문지 Vol.22 No.4

이 연구의 목적은 백색 LED 조명의 광학적 특성 중에서 색온도 특성에 따른 사람들의 감성 반응을 평가하는데 있다. 이를 위해 백색 LED 모듈을 시제품으로 만들고, 감성 평가용 모형을 제작하여 색온도에 따른 감성 반응을 평가 분석하였다. 감성 평가에는 15개의 문항에 대하여 5단계 SD법으로 하였으며, 신뢰도 검증과 타당성 검증을 통하여 조명과 관련이 있는 3개의 요인을 선정하였다. 1요인을 활동성 요인으로 명명하였고, 2요인을 안정성 요인으로 명명하였으며, 3요인을 역량성 요인으로 명명하였다. 감성 평가를 분석하기 위해 종속 변수는 감성 평가 점수로 하였으며, 독립 변수는 색온도로 분석하였다. 이 연구에서 얻어진 결과를 정리하면 다음과 같다. 형광등과 비교한 백색 LED 조명에 있어서 색온도에 따른 사람들에 대한 감성 평가는 활동성 요인과 역량성 요인에서는 색온도가 8,300[K]인 MA₃ 조명을 높게 평가하였으며, 안정감 요인에서는 색온도가 3,800[K]인 MA₁ 조명을 높게 평가하였다. The aim of this study is to investigate the sensitivity evaluation of human beings in reacting to the correlated color temperature of the optical properties of white LED lighting. For the sake of this study, white light-emitting diode modules have been fabricated, their correlated color temperature have been measured, test cabinets for the sensitivity evaluation have been constructed with the white LED modules, and their sensitivity reactions on the test cabinets have been evaluated and analyzed. The sensitivity reaction has been evaluated by the semantic differential method with 15 selected questions, and the reliability and the content validity of their lighting have been analyzed to 3 factors which are the activity as the first factor, the stability as the second one, the potency as the third one, respectively, For the data analysis on the sensitivity reaction, the dependent variable is the score of the sensitivity evaluation and the independent one is the correlated color temperature of the test module. The results of this study is as follows: In the case of the sensitive evaluation on the activity and the potency in the white LED lighting compared with the fluorescent lamp, the subjects have made higher mark on MA₃ with 8,300[K], and in the factor of the stability, they have made higher mark on MA₁ with 3,800[K].