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차인호,김용환,Cha, In-ho,Kim, Yong-hwan 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.2
The objects of the present study were to establish the method of purification, subunit dissociation of verotoxin-2 (VT2) produced by Escherichia coli O157:H7, and to investigate the characteristics of purified verotoxin-2 such as molecular weight and composition of amino acid. The results were summerized as follows; Verotoxin-2 was extracted by addition of polymyxin B sulfate into bacterial cell lysate prepared from Escherichia coli O157:H7(KSC109). As an initial step, the bacterial cell lysate was precipitated with 30% saturated ammonium sulfate. The precipitated crude toxin was then subjected to anion-exchange, chromatofocusing and cation-exchange chromatography. Using this scheme, we obtained highly purified toxin with a specific activity of $1.1{\times}10^9$ $CD_{50}/mg$. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) for purified VT2 showed two protein bands. The upper band, approximately 32 Kd, was supposed as A subunit and the lower band, approximately 7.7 Kd, was supposed as B subunit. When the toxin was separated in the subunit-dissociating solution, two peaks emerged with retention times of 15 and 28 min by HPLC. These peaks represented A subunit and B subunit, respectively. The amino acid composition of purified VT2 were made up in order of glutamic acid, histamine, asparaginic acid, histidine, lysine, alanine and leucine etc. The largest amount among the amino acid composing VT2 was methionine.
차인호,김용환,Cha, In-ho,Kim, Yong-hwan 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.2
Three and 2 strains of E coli O157 were isolated from fecal materials of cattle (390) and pigs (420) in Korea, respectively. One strain of O157:H7 isolated from cattle and 2 strains of O157:H7 isolated from pigs were identified as verotoxin-1 (VT-1) produing strains and 2 strains (O157:H7 and O157:H-) isolated from cattle were identified as verotoxin-2 (VT-2) producing strains by neutralization test on HeLa and Vero cells. Culture supernatants of the isolates were cytotoxic to HeLa and Vero cells. The levels of cytotoxin produced by isolates were $10^2{\sim}10^4$ cytotoxic dose($CD_{50}$)/ml. Also, VT-2-converting bacteriophage was isolated from KSC109 strain which had been isolated from cattle. Molecular weight of the phage DNA was determined as approximately 45 Kb in 0.8% agarose gel electrophoresis, and morphology of the phage stained with phosphotungstic acid was observed by transmissible electron microscopy.
차인호,김희진,정영수,이충국,정인혁,Cha, In-Ho,Kim, Hee-Jin,Jeong, Young-Soo,Yi, Choong-Kook,Chung, In-Hyuk 대한악안면성형재건외과학회 1998 Maxillofacial Plastic Reconstructive Surgery Vol.20 No.3
To clarify the clinical utility of the calvarial bone graft in the maxillofacial reconstruction, we performed on anatomical study by measuring the regional thickness of the parietal bone on 17 Korean adult dry skulls. Before the sectioning the calvarium, the anatomical landmarks were marked on each specimens. And then we measured the total thickness of the parietal bone, the thickness of the outer and inner cortical plates on various points in each sections of parietal bones using a digital caliper under the stereomicroscope. The total thickness of the parietal bone was ranged from 5.17mm to 7.50mm, and there were no statistical difference in the total thickness of the parietal bone on the same points bilaterally. But there was a tendency that the thickness of the parietal bone was thicker toward to the lambda point than the coronal suture area. At the other hand, the thickness of the outer and inner plate of the parietal bone was the thickest at the first point of the right aspect on the line 1, the first point of the left aspect on the line 5, respectively. In conclusion, this study showed that the donor site of the parietal bone for the maxillofacial reconstruction should be located at more posterior and medial area of the parietal bone than the prevalent known donor site.
구강악안면외과 영역에서의 MIDFACIAL DEGLOVING APPROACH의 유용성
차인호,윤현중,이의웅,Cha, In-Ho,Yoon, Hyun-Joong,Lee, Eui-Wung 대한악안면성형재건외과학회 1998 Maxillofacial Plastic Reconstructive Surgery Vol.20 No.3
Midfacial degloving approach의 장점으로는 비강, 비중격, 상악동, 사골동, 접형골동, 비인두 부위 병소의 접근에 좋은 시야를 제공하며, 수술방법이 기존의 방법에 비해 수월하고, 두피관상 절개법, 측두부 절개법, 구개부 절개법과 함께 사용하여 수술시야를 넓힐 수 있으며, 안면부에 나타나는 흉터가 없어 심미적으로 만족스러우며 특히, Keloid 체질에서 유용하다는 것을 들 수 있다. 이에 저자 들은 구강악안면외과 영역에서도 중안면부 외상, 두개안면부 기형 처치, 상악골 절제술을 요하는 경우, 상악골과 비골의 동시 수술을 요하는 경우 등에 midfacial degloving approach가 유용한 수술 접근 방법으로 사료되었다. In the treatment of maxillary lesion including a maxillary sinuses, most of oral and maxillofacial surgeons have used the vestibular incision or the Weber-Fergusson incision. However, the vestibular incision has disadvantage which it provides a rather limited exposure and the Weber-Fergusson incision leaves visible scar in the midface. Furthermore, because the scar is confined on unilateral side only, the technique is hesitated to apply especially in children, younger patients and women. Since Casson first introduced midfacial degloving technique, this approach has been used frequently to treat the lesion on nasal cavity, nasopharynx, skull base and paranasal sinuses by ENT surgeons. But, we think this technique can be used usefully in oral and maxillofacial regions. So, we experienced favorable results which it provided a proper exposure, no visible facial scar and it could be used on bilateral midfacial lesions.
차인호(In Ho Cha),한지훈(Ji Hoon Han),이정구(Jeong Gu Lee),이충국(Choong Kook Lee),권칠성(C. S. Gwon),오성섭(Seong Sub Oh),김일현(Il Hyun Kim),정세용(Sei Yong Chung) 대한악안면성형재건외과학회 1987 Maxillofacial Plastic Reconstructive Surgery Vol.9 No.1
This is a case of bilateral bony ankylosis of temporomandibular joint with severe micrognathia corrected by use of metallic condylar prosthesis after ramal surgery and coronoidotomy and then body osteotomy with graft of Pyrost(R) and chin augmentation with Medpor(R). The Postoperative results were good functionally and esthetically.
차인호(Cha inho),이제균(Lee jegeun),김용열(Kim yongyeol),이종익(Lee jongik),김한일(Kim hanil) 한국유체기계학회 2007 유체기계 연구개발 발표회 논문집 Vol.- No.-
Intake pump for waterworks is badly damaged by a small amount of cavitation because of variable water quality and severe operation conditions. In general, the required NPSH for reduced cavitation can be provided by inlet condition, supply air, change pump and inducer. But once the pump has been built and installed there is little that can be done to reduce cavitation damage. In this study, we analysed the cavitation of paldang intake pump and intended to avoid the same phenomena.
Eacherichie coli O157: H7 으로부터 분리한 Bacteriophages의 특성
차인호(In Ho Cha),이영춘(Yeung Chun Lee),김용환(Young Hwan Kim) 한국예방수의학회 1996 예방수의학회지 Vol.20 No.1
Bacteriophage encoding toxin gene Was lysogenized from Escherichia coli O157:H7 by the method of UV irradiation. This phage was examined its morphology by electronmicroscopy and was investigated its stability to temperature, pH stability, inactivation by UV irradiation and characteristics of phage DNA. The morphology of bacteriophage lysogenized from verotoxin-2-producing Escherichia coli had head and tail as typical bacteriophage, The phage was stable in wide pH range (pH 6~10), and optimal pH for survival was pH 7.0. In temperature stability, phage was most stable at 35℃ and mostly inactivated by UV irradiation for 100 sec. Adsorption rates of the phage on lawn cell (E.coli C600) was represented beyond 90% by mixed incubation for 35 min.
차인호(In-Ho Cha) 대한치과의사협회 2011 대한치과의사협회지 Vol.49 No.3
Oral precancerous lesion is a morphologically altered tissue in which oral cancer is more likely to occur than is apparently normal counterpart. As dentists always do oral examination and dental treatment, with fundamental knowledge and attention of this lesion, it is relatively easy to find one. If followed by proper treatment and management, it is possible to minimize its oral cancer progression, or at least delay it. Even if it were to progress to oral cancer, very early detection is possible. However, no specific biomarkers are present at the moment that could reveal oral precnacerous lesion that is high risk of oral cancer progression. Since early detection of oral cancer followed by treatment could show good prognosis with just a simple ablative surgery. Dentists should also instruct people to avoid risk factor related oral cancer progression and take natural compound having anticancer effect. Hereby, As a primary care givers, dentists play an important role in prevention of oral cancer.
커피매장에서 빛의 수평-수직-입체적 요소에 따른 조명디자인과 사용자행태의 관계성에 관한 연구
차인호 ( In Ho Cha ),박일권 ( Il Kwun Park ) 디자인융복합학회 2014 디자인융복합연구 Vol.13 No.4
빛과 공간의 상관관계를 통해 형성되는 공간의 인상은 빛의 수평-수직-입체적인 요소에 의해 통합적으로 연출된다. 커피매장은 공간의 목적상 상업공간으로서 고객유입과 매출증진에 기여할 수 있도록 빛의 계획이 필요한 곳이며, 더불어 방문객의 쾌적한 휴식과 커뮤니케이션을 위한 문화공간으로서 빛의 양적 측면보다 질적 개선에 보다 중점을 둔 조명계획이 우선된다. 이에 본 연구는 실제 브랜드 커피매장에서 빛의 수평-수직-입체적 요소를 분석하고 빛의 요소에 따른 사용자행태를 관찰하여 빛공간이 사용자행태에 미치는 영향을 알아보았다. 연구결과 체계적인 조명계획에 따른 커피매장에서는 사용자 행태가 조명계획에 상응하는 관계성을 보이고 있었으며, 그렇지 않은 커피매장에서는 빛의 구성보다 가구적인 요소나 경관적인 요소에 의해 사용자 행태가 결정되거나 혼재되는 양상을 보여 커피매장 내에서 빛의 요소에 따라 사용자행태가 달라짐을 알 수 있었다. An Impression of space to be formed by the correlation between light and space is syntagmatically created by the horizontal-vertical-three dimensional elements of the light. A coffee shop as a commercial space is the place to need a lighting designfor increase of sales and detention of customer. The qualitative improvement of light is more important than the quantitative it for amenity space and communication for the customer. In thisresearch, we conducted a research on the effect of light on the user’s behavior by analyzing the Horizontal-Vertical-Three Dimensional elements of light and observing the user’s behavior in the brand coffee shops. Consequently, this study showed theconformity of user’s behavior on lighting space in the coffee shop with lighting design however, the coffee shop without it showed the aspect to be decided by the elements of the furniture or landscape than light. It means that the user’s behavior depends on the elements of light.