배양된 메산지움세포에서 당수송체-1 발현에 대한 Protein Kinase C 억제제의 효과

차대룡,김신곤,김난희,최동섭,백세현,최경묵,신동현,박이병,김동림 대한당뇨병학회 2001 Diabetes and Metabolism Journal Vol.25 No.3

Background: Recent studies have suggested that increased glucose uptake via GLUT1 may be a major determinant of glucose utilization and extracellular matrix formation in mesangial cells. This study was to evaluate the effect of protein kinase C inhibitor on glucose transporter-1 (GLUTI) expression in cultured rat mesangial cells. Methods: The GLUT1 expression was evaluated in mesangial cells exposed to various glucose concentrations of media (5.5 mM, 15 mM or 30 mM) and incubation times (6 hr, 24 hr or 72 hr) by semiquantitative RT-PCR and western blot analysis. The effect of protein kinase C (PKC) inhibitor, calphostin C and phorbol 12-myristate 13-acetate (PMA) on GLUT1 expression was also evaluated under the same conditions. Results: The GLUTI mRNA expressions were significantly increased in MG (15 mM) and HG (30 mM) than those in NG (5.5 mM) with incubation of 6 hr, 24 hr and 72 hr, respectively. In HG media, the GLUT1 mRNA expression with incubation of 24 hr and 72 hr were significantly increased than that with incubation of 6 hr, respectively. In HG media, the GLUTI mRNA expressions were significantly reduced in calphostin C and PMA treated groups compared with those in untreated groups. In western blot analysis of HG media, GLUT1 proteins were identified in PMA- or calphostin C-untreated group and PMA b hr treated group, but not identified in PMA 24 hr treated group and in calphostin C-treated groups with incubation of 6 hr and 24 hr. Conclusion: PKC inhibitors decrease glucose-induced GLUT1 expression under high glucose concentration in mesanglal cells. These results suggest that PKC pathway may regulate GLUT1 expression under high glucose concentration in cultured rat mesangial cells.

Mineralocorticoid receptor blockade for renoprotection

차대룡 대한신장학회 2018 Kidney Research and Clinical Practice Vol.37 No.3

Extensive animal and clinical studies have demonstrated that inhibition of the renin-angiotensin-aldosterone system (RAAS) has a widespread protective role in various tissues. Although angiotensin II is the primary mediator of RAAS in the kidney, the effects of blockade with angiotensin-converting enzyme inhibitors (ACEi) or angiotensin II receptor antagonists (ARB) are limited with respect to prevention of end-stage renal disease.

Interleukin-6 signaling in podocyte hypertrophy

차대룡 대한신장학회 2016 Kidney Research and Clinical Practice Vol.35 No.4

Diabetic kidney disease is characterized by increased albuminuria associated with structural changes, such as thickening of the glomerular basement membrane, mesangial expansion, and podocyte depletion followed by glomerulosclerosis. Glomerulosclerosis is preceded by early hypertrophic changes in the glomerulus [1].

Otsuka Long-Evans Tokushima Fatty(OLETF)쥐에서 Glycosaminoglycan 투여가 N-acetyl-β-D-Glucosaminidase 배설에 미치는 영향

차대룡,김난희,최동섭,백세현,최경묵,김동림,한상엽 대한당뇨병학회 2000 Diabetes and Metabolism Journal Vol.24 No.5

Background: Increased loss of proteoglycan (PG) characterized by an increased loss of anionic charges in the basement membrane has been considered as one of main factors causing urinary loss of albumin. The glycosaminoglycans (GAGs) are linear polymers of repeated disaccharides and the GAG chains are covalently bound to core proteins, forming proteoglycans. It is known that urinary N-acetyl-β -D-glucosaminidase (NAG) excretion is a sensitive marker of renal damage and is increased before other renal functional parameters. The aim of this study was to investigate whether GAG treatment is capable of influencing urinary protein and NAG excretion in Otsuka Long-Evans Tokushima Fatty (OLETF) rats which are known as type 2 diabetic animal model. Methods: Fifteen male OLETF rats and twenty male Long-Evans Tokushima Otsuka (LETO) rats were used for this study. LETO rats are non-diabetic control rats. All OLETF rats were randomly assigned to 2 groups: control group (n=10) given only . tap water and GAG group (n=5) feeding with GAG 10 mg/kg from 7 weeks to 55 weeks of age. Measurement of body weight, blood glucose, serum BUN and creatinine was performed periodically. 24-hour urine collection for measurement of urinary protein and NAG excretion was done at 17, 25, 37, 46, 55 weeks of age. Results: 1) OLETF rats showed higher body weight, blood glucose, 24-hour urinary protein and NAG excretion compared with LETO rats. But serum concentration of BUN and creatinine were not different between OLETF and LETO rats. 2) GAG-treated OLETF rats exhibited lower urinary protein/creatinine excretion (17.48±0.50 vs 22.49±0.11 mg/mg Cr, p $lt; 0.05) and NAG (17.40±5.94 vs 43.73±7.44 nmol/h/mg Cr, p $lt; 0.05) excretion compared with non-treated OLETF rats. But body weights, blood glucose, serum concentration of BUN and creatinine were not different between GAG-treated OLETF rats and non-treated OLETF rats. Conclusion: 1) The urinary excretion of NAG may be a possible early marker of diabetic nephropathy in OLETF rats. 2) Urinary protein and NAG excretion were decreased in the GAG-treated OLETF rats. GAG seems to have a protective effect against development of diabetic nephropathy.

Where do we stand on human diabetic nephropathy?

차대룡 대한신장학회 2013 Kidney Research and Clinical Practice Vol.32 No.3

The prevalence of type 2 diabetes is rising rapidly in Asian populations due to lifestyle changes and increased life expectancy. With the rapidly increasing number of patients with diabetes worldwide, diabetic nephropathy (DN) is becoming one of the most common causes of renal disease. Indeed, approximately 20–40% of diabetic patients develop DN [1].

혈액투석시 Double Lumen Silicone Rubber Catheter ( = Permcath ) 사용의 임상적 경험

차대룡(Dae Ryong Cha),김선숙(Sun Sook Kim),이영호(Young Ho Lee),권영주(Young Joo Kwon),조원용(Won Yong Cho),김형규(Hyoung Kyu Kim),선경(Kung Sun),김정숙(Jung Sook Kim),함인귀(In Gui Ham),김미경(Mi Kyung Kim) 대한내과학회 1994 대한내과학회지 Vol.46 No.4

Background: Repeated, long-term access of the vascular system is a prerequisite for successful extended care of the patients with end stage renal disease (=ESRD) treated with hemodialysis. Despite recent technical advances in percutaneous venous cannulation, vascular access remains a major problem in patients requiring acute Hemodialysis. Although the subclavian cannula has gained a large clinical acceptance in recent years, it carries specific risks such as hemothorax, pneumothorax, venus stenosis, thrombus formation and infection. Recently, a double-lumen, central venous catheter made of Silicone Rubber (=Perm cath) has developed for use as a vascular access device, This device is particularly useful in patients who have exhausted other vascular access sites or who have severe cardiovascular disease. Methods: A total of 23 dual-lumen silicone rubber catheters were placed through the internal jugular vein in 23 patients as a vascular access at our institution during the period of April, 1992 through November, 1992. The perm-cath (Hemocath, Quinton, Seattle) is 36 Cm long and each lumen has an internal diameter of 2 mm. Implantation of the catheters occurred in the operating room and under strict aseptic conditions through the right internal jugular vein. A 10 cm subcutaneous tunnel was then created, which extended from the in-cison to a point 2 cm above the ipsilateral clavicle. The catheter was passed out through the upper portion of the subcutaneous tunnel with the Dacron cuff placed 2 cm from the lower end of the tunnel, acting as both an anchor and a barrier to infection. The tip of the catheter was inserted through an internal jngular vein terminating in the right atrium under EKG monitoring. Each lumen of the catheter was filled with 1000 units of heparin sodium (1.5 ml in venous line, 1.5 ml in arterial line) and capped. Results : The mean duration of catheter use was 24±16 days (8 to 119 days), and the complication of perm-cath occurred in 3 cases such as venous thrombosis in 1 case, catheter exit site infection in 1 case, and exit site hematoma in 1 case. During hemodialysis, blood flow rate ranged from 190 to 313ml/min (mean:235±26ml/ min), and venous retrun pressure ranged from 20 to 150 mmHg(mean:65±16mmHg). Total 14 catheters were removed during treatment and the causes of catheter removal were patient death in 8 cases, venous thrombosis 1, recovery from acute rena1 failure 2, fistula maturation 2, infection 1. Conclusion: Internal jugular vein cannulation with silastic catheter which offers a new percutaneous method was provided safe and reliable as the temporary central vein access. The catheters are well tolerated by the patients and have the advantages of immediate use after placement, high blood flow rates, no repetitive venipuncture, and no cardiac dysfunction. Permcath is particulary useful in patients who have exhausted other vascular access site, severe cardiovascular disease, and terrified by repetitive venipuncture. Though our initial experience has been favorable, there will be needed to evaluate the outcome of permcath over longer period of time.

허혈성 신손상후 c - fos 및 Epidermal Growth Factor 유전자 발현양상

차대룡(Dae Ryong Cha),이영호(Young Ho Lee),장미경(Mi Kyung Jang),김난희(Nan Hee Kim),구자룡(Ja Ryong Koo),권영주(Young Joo Kwon),조원용(Won Yong Cho),김형규(Hyoung Kyu Kim),김창수(Chang Soo Kim) 대한내과학회 1994 대한내과학회지 Vol.47 No.6

Objectives: Acute renal failure (ARF) is a syndrome that can he broadly defined as rapid deterioration of renal function resulting in the accumulation of nitrogenous wastes such as urea and creatinine. The incidence of ARF will most likely increase in the future as a predictable by-product of the continuous advances in surgical techniques and pharmacotherapy. The severity of renal dysfunction after ARF depends on the exent of the initial renal damage as well as the pace of repair process, and the regeneration of tubular epithelial cell is essential in the recovery of ARF. Recently several studies reveal the importance of specific growth factor such as epidermal growth factor (EGF), transforming growth factor-alpha (TGFα) and the expression of these genes is increased during the recovery stage of ARF. To evaluate the expression of c-fos and EGF genes involved in cellular proliferation during acute ischemic renal failure, author performed the northern and dot hybridization of renal tissue at different reperfusion time in the ischemic ARF rats. Methods: The experimental animals were divided into three groups. Group I (n=3) was control without any procedure, group II (n=3) was sham operation group (bilateral flank incision and decapsulation were made without renal artery clamping), group III (n=15) was ischemic ARF model by right nephrectomy and left renal artery clamping for 40 minutes. In ischemic group (Group III), rats were divided into three subgroups according to reperfusion time such as 1, 24, 72 hours (IIIa, IIIb, IIIc in each). For these studies, the non-ischemic right kidney removed at the time of initial surgery served as a paired control. Becaus of inter-animal variation in mRNA content for specific genes, five rats were included for each time period. In all cases, whole blood were collected for measurement of serum creatinine at each reperfusion time. Total renal RNA was purified from intact whole kidneys by deproteinization with guanidinium isothiocyanate and phenol/chloroform/isoamyl alcohol. After the isolation of RNA, electrophoresis were done in a 1% agarose gel containing 20 mM MOPS, 1 mM EDTA, 5 mM Na acetate PH 7.0 and 2.2M formaldehyde and confirmed the intact RNA by the 18 S and 28 S ribosomal RNA. RNA was transferred to nylon membrane via vaccum transfer and then hybridization were performed at 65℃ with isotope labelled probes for 24hours, Autoradiographs were obtained and quantitated by computer-assisted dual- wave length flying spot scanner (CS-9000) at 530 nm. Results: In the control group, the serum creatinine was 0.9±0.2 mg/dl; in the sham operation group 0.8±0.3 mg/dl; in the ischemic group after 1 hour reperfusion, the serum creatinine was 0.9±0.3 mg/dl; In the ischemic group after 24hous and 72 hours reperfusion, the serum creatinine was 1.9±0.5 mg/dl and 3.6±1.4 mg/dl respectively. The mean serum creatinine level was statistically significant between control and post-ischemic 24, 72 hours reperfusion group (p<0.05). c-fos gene was rapidly induced by renal ischemia with peak after 60 minutes of reflow and 24 hours later c-fos message was markedly decreased. The expression of c-fos gene was. more markedly increased in the more severe ischemic injury. EGF gene expression was markedly decreased after 1 hour of reflow and substantially increased in activity ?2 hours after ischemia. Conclusion: From the above findings, c-fos gene expression is rapidly induced by ischemia and this gene expression is essential in the recovery phase of acute ischemic renal failure. EGF gene expression substantially increased in activity is probablely associated with renal epithelial cell proliferation. Although the specific roles of c-fos and EGF genes in tissue recovery are not known, the expression of these genes may be play an important role in the recovery phase of acute ischemic renal failure.

로봇용 고정밀 감속기의 각도전달오차 평가방법에 대한 연구

차대룡(Dea-Ryong Cha),김경식(Kyeong-Sik Kim),안호진(Ho-Jin An),백두산(Du-San Baek),백승연(Seung-yeon Baek),이현(Hyeon Lee),김선혁(Sun-Hyuck Kim) 대한전기학회 2024 대한전기학회 학술대회 논문집 Vol.2024 No.7

INH 및 β-Lactam 항생제에 의해 유발된 급성간질성 신염 2예

차대룡 ( Cha Dae Lyong ),이영호 ( Lee Yeong Ho ),김선숙 ( Kim Seon Sug ),권영주 ( Kwon Yeong Ju ),조원용 ( Jo Won Yong ),김형규 ( Kim Hyeong Gyu ),원남희 ( Won Nam Hui ) 대한내과학회 1993 대한내과학회지 Vol.44 No.3

Acute Interstitial Nephritis is a common disorder characterized by a spectrum of clinical manifestations ranging from asymptomatic urinary abnormalities to acute oliguric renal failure. The histologic picture of AIN is a prominent inflammatory cell infiltrate within the renal interstitium, and major glomerular and vascular lesions are absent. In recent years, AIN has been recognized most commonly as a complication after exposure to a variety of drugs associated with newly introduced chemotherapeutic agent, especially antibiotics. Recently, we experienced of two cases of INH and β-lactam antibiotic induced acute interstitial nephritis, and β-lactam associated case presented as anuria for several days. The first case was 59 years old male who experienced acute subdural hematoma 2 months ago and developed a generalized edema and anuria after treatmeut with β-lactam antibiotics (ceradolan 3.0g/day for 7 days) to control urinary tract infection. Renal biopsy revealed an interstitial nephritis with mononuclear cell infiltration in the interstitium, renal tubular atrophy with degenerative change without any abnormal findings in the glomerulus. The second case was 59 years old male who has been suffered from bladder cancer 1 month ago and performed surgical resection and chemotherapy. He complained lower abdominal pain, fever, chilling sensation 1 week before admission. Physical examination and laboratory finding showed the picture of acute peritonitis. INH(600mg daily) was introduced for 1 week as diagnostic purpose, and he presented as a progressive azotemia and oliguria. Renal biopsy revealed the same nature as case 1, and focal deposits of eosinophil were found in electron microscope. In both cases, renal function has been recovered with conservative management including the discontinuation of the antibiotics and steroid administration.

신 증후 출혈열에 항 호중구 형질항체 검사의 임상적 의의

차대룡(Dae Ryong Cha),김선숙(Sun Sook Kim),이영호(Young Ho Lee),권영주(Young Ju Kwon),조원용(Won Yong Cho),김형규(Hyoung Kyu Kim),조윤정(Yoon Jeong Cho) 대한내과학회 1992 대한내과학회지 Vol.43 No.4

HFRS seems to be systemic vasculitis such as antineutrophil cytoplasmic autoantibody (ANCA) associated disease. Author purposed to investigate the role of ANCA in the pathogenesis of HFRS and its relation with the clinical findings of HFRS. Methods: Author measured ANCA by indirect immunofluorescent method using alcohol-fixed neutrophils as substrate in 14cases of serologically proven HFRS in the patients serum of oliguric and diuretic phase. Results: 1) The study population consisted of 10oliguric and 4nonoliguric HFRS cases. There were no significant difference in clinical manifestations at admission. 2) ANCA were detected positively in 3 of 10oliguric HFRS cases (2 in oliguric stage, 1 in diuretic stage), and all of nonoliguric HFRS cases were negative. 3) Gross hematuria and proteinuria at the time of admission were showed in all of positive cases, and this abnormal urinary fir. dings spontaneously resolved during the hospital course. There were no significant difference in clinical course and laboratory findings between ANCA positive and negative groups. 4) By indirect immunofluorescence microscopy using alcohol-fixed neutrophils as substrate, all positive cases showed typical cytoplasmic staining pattern (C-ANCA) and none had extrarenal disease. 5) All three ANCA positive cases showed negative in follow up measurement of ANCA 6months later after recovery of disease. Conclusion: From the above findings, pathophysiology of vasculitis in hemorrhagic fever with renal syndrome may be related with ANCA in such eases, but further studies are requested in detail.