http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 차대룡 (검색결과 216 건)
- 무료
- 기관 내 무료
- 유료
-
-
INH 및 β-Lactam 항생제에 의해 유발된 급성간질성 신염 2예
차대룡,이영호,김선숙,권영주,조원용,김형규,원남희 대한내과학회 1993 대한내과학회지 Vol.44 No.3
저자들은 β-lactam 항생제 및 INH 사용후 신조직 검사로 확진된 급성간질성 신염 2예를 경험하였기에 문헌고찰과 함께 보고하였다. Acute Interstitial Nephritis is a common disorder characterized by a spectrum of clinical manifestations ranging from asymptomatic urinary abnormalities to acute oliguric renal failure. The histologic picture of AIN is a prominent inflammatory cell infiltrate within the renal interstitium, and major glomerular and vascular lesions are absent. In recent years, AIN has been recognized most commonly as a complication after exposure to a variety of drugs associated with newly introduced chemotherapeutic agent, especially antibiotics. Recently, we experienced of two cases of INH and β-lactam antibiotic induced acute interstitial nephritis, and β-lactam associated case presented as anuria for several days. The first case was 59 years old male who experienced acute subdural hematoma 2 months ago and developed a generalized edema and anuria after treatmeut with β-lactam antibiotics (ceradolan 3.0g/day for 7 days) to control urinary tract infection. Renal biopsy revealed an interstitial nephritis with mononuclear cell infiltration in the interstitium, renal tubular atrophy with degenerative change without any abnormal findings in the glomerulus. The second case was 59 years old male who has been suffered from bladder cancer 1 month ago and performed surgical resection and chemotherapy. He complained lower abdominal pain, fever, chilling sensation 1 week before admission. Physical examination and laboratory finding showed the picture of acute peritonitis. INH(600mg daily) was introduced for 1 week as diagnostic purpose, and he presented as a progressive azotemia and oliguria. Renal biopsy revealed the same nature as case 1, and focal deposits of eosinophil were found in electron microscope. In both cases, renal function has been recovered with conservative management including the discontinuation of the antibiotics and steroid administration.
-
-
배양된 메산지움세포에서 당수송체-1 발현에 대한 Protein Kinase C 억제제의 효과
차대룡,김신곤,김난희,최동섭,백세현,최경묵,신동현,박이병,김동림 대한당뇨병학회 2001 Diabetes and Metabolism Journal Vol.25 No.3
Background: Recent studies have suggested that increased glucose uptake via GLUT1 may be a major determinant of glucose utilization and extracellular matrix formation in mesangial cells. This study was to evaluate the effect of protein kinase C inhibitor on glucose transporter-1 (GLUTI) expression in cultured rat mesangial cells. Methods: The GLUT1 expression was evaluated in mesangial cells exposed to various glucose concentrations of media (5.5 mM, 15 mM or 30 mM) and incubation times (6 hr, 24 hr or 72 hr) by semiquantitative RT-PCR and western blot analysis. The effect of protein kinase C (PKC) inhibitor, calphostin C and phorbol 12-myristate 13-acetate (PMA) on GLUT1 expression was also evaluated under the same conditions. Results: The GLUTI mRNA expressions were significantly increased in MG (15 mM) and HG (30 mM) than those in NG (5.5 mM) with incubation of 6 hr, 24 hr and 72 hr, respectively. In HG media, the GLUT1 mRNA expression with incubation of 24 hr and 72 hr were significantly increased than that with incubation of 6 hr, respectively. In HG media, the GLUTI mRNA expressions were significantly reduced in calphostin C and PMA treated groups compared with those in untreated groups. In western blot analysis of HG media, GLUT1 proteins were identified in PMA- or calphostin C-untreated group and PMA b hr treated group, but not identified in PMA 24 hr treated group and in calphostin C-treated groups with incubation of 6 hr and 24 hr. Conclusion: PKC inhibitors decrease glucose-induced GLUT1 expression under high glucose concentration in mesanglal cells. These results suggest that PKC pathway may regulate GLUT1 expression under high glucose concentration in cultured rat mesangial cells.
-
차대룡,김난희,최동섭,백세현,최경묵,김동림,한상엽 대한당뇨병학회 2000 Diabetes and Metabolism Journal Vol.24 No.5
Background: Increased loss of proteoglycan (PG) characterized by an increased loss of anionic charges in the basement membrane has been considered as one of main factors causing urinary loss of albumin. The glycosaminoglycans (GAGs) are linear polymers of repeated disaccharides and the GAG chains are covalently bound to core proteins, forming proteoglycans. It is known that urinary N-acetyl-β -D-glucosaminidase (NAG) excretion is a sensitive marker of renal damage and is increased before other renal functional parameters. The aim of this study was to investigate whether GAG treatment is capable of influencing urinary protein and NAG excretion in Otsuka Long-Evans Tokushima Fatty (OLETF) rats which are known as type 2 diabetic animal model. Methods: Fifteen male OLETF rats and twenty male Long-Evans Tokushima Otsuka (LETO) rats were used for this study. LETO rats are non-diabetic control rats. All OLETF rats were randomly assigned to 2 groups: control group (n=10) given only . tap water and GAG group (n=5) feeding with GAG 10 mg/kg from 7 weeks to 55 weeks of age. Measurement of body weight, blood glucose, serum BUN and creatinine was performed periodically. 24-hour urine collection for measurement of urinary protein and NAG excretion was done at 17, 25, 37, 46, 55 weeks of age. Results: 1) OLETF rats showed higher body weight, blood glucose, 24-hour urinary protein and NAG excretion compared with LETO rats. But serum concentration of BUN and creatinine were not different between OLETF and LETO rats. 2) GAG-treated OLETF rats exhibited lower urinary protein/creatinine excretion (17.48±0.50 vs 22.49±0.11 mg/mg Cr, p $lt; 0.05) and NAG (17.40±5.94 vs 43.73±7.44 nmol/h/mg Cr, p $lt; 0.05) excretion compared with non-treated OLETF rats. But body weights, blood glucose, serum concentration of BUN and creatinine were not different between GAG-treated OLETF rats and non-treated OLETF rats. Conclusion: 1) The urinary excretion of NAG may be a possible early marker of diabetic nephropathy in OLETF rats. 2) Urinary protein and NAG excretion were decreased in the GAG-treated OLETF rats. GAG seems to have a protective effect against development of diabetic nephropathy.
-
-
-
-
-
허혈성 신손상후 c - fos 및 Epidermal Growth Factor 유전자 발현양상
차대룡(Dae Ryong Cha),이영호(Young Ho Lee),장미경(Mi Kyung Jang),김난희(Nan Hee Kim),구자룡(Ja Ryong Koo),권영주(Young Joo Kwon),조원용(Won Yong Cho),김형규(Hyoung Kyu Kim),김창수(Chang Soo Kim) 대한내과학회 1994 대한내과학회지 Vol.47 No.6
N/A Objectives: Acute renal failure (ARF) is a syndrome that can he broadly defined as rapid deterioration of renal function resulting in the accumulation of nitrogenous wastes such as urea and creatinine. The incidence of ARF will most likely increase in the future as a predictable by-product of the continuous advances in surgical techniques and pharmacotherapy. The severity of renal dysfunction after ARF depends on the exent of the initial renal damage as well as the pace of repair process, and the regeneration of tubular epithelial cell is essential in the recovery of ARF. Recently several studies reveal the importance of specific growth factor such as epidermal growth factor (EGF), transforming growth factor-alpha (TGFα) and the expression of these genes is increased during the recovery stage of ARF. To evaluate the expression of c-fos and EGF genes involved in cellular proliferation during acute ischemic renal failure, author performed the northern and dot hybridization of renal tissue at different reperfusion time in the ischemic ARF rats. Methods: The experimental animals were divided into three groups. Group I (n=3) was control without any procedure, group II (n=3) was sham operation group (bilateral flank incision and decapsulation were made without renal artery clamping), group III (n=15) was ischemic ARF model by right nephrectomy and left renal artery clamping for 40 minutes. In ischemic group (Group III), rats were divided into three subgroups according to reperfusion time such as 1, 24, 72 hours (IIIa, IIIb, IIIc in each). For these studies, the non-ischemic right kidney removed at the time of initial surgery served as a paired control. Becaus of inter-animal variation in mRNA content for specific genes, five rats were included for each time period. In all cases, whole blood were collected for measurement of serum creatinine at each reperfusion time. Total renal RNA was purified from intact whole kidneys by deproteinization with guanidinium isothiocyanate and phenol/chloroform/isoamyl alcohol. After the isolation of RNA, electrophoresis were done in a 1% agarose gel containing 20 mM MOPS, 1 mM EDTA, 5 mM Na acetate PH 7.0 and 2.2M formaldehyde and confirmed the intact RNA by the 18 S and 28 S ribosomal RNA. RNA was transferred to nylon membrane via vaccum transfer and then hybridization were performed at 65℃ with isotope labelled probes for 24hours, Autoradiographs were obtained and quantitated by computer-assisted dual- wave length flying spot scanner (CS-9000) at 530 nm. Results: In the control group, the serum creatinine was 0.9±0.2 mg/dl; in the sham operation group 0.8±0.3 mg/dl; in the ischemic group after 1 hour reperfusion, the serum creatinine was 0.9±0.3 mg/dl; In the ischemic group after 24hous and 72 hours reperfusion, the serum creatinine was 1.9±0.5 mg/dl and 3.6±1.4 mg/dl respectively. The mean serum creatinine level was statistically significant between control and post-ischemic 24, 72 hours reperfusion group (p<0.05). c-fos gene was rapidly induced by renal ischemia with peak after 60 minutes of reflow and 24 hours later c-fos message was markedly decreased. The expression of c-fos gene was. more markedly increased in the more severe ischemic injury. EGF gene expression was markedly decreased after 1 hour of reflow and substantially increased in activity ?2 hours after ischemia. Conclusion: From the above findings, c-fos gene expression is rapidly induced by ischemia and this gene expression is essential in the recovery phase of acute ischemic renal failure. EGF gene expression substantially increased in activity is probablely associated with renal epithelial cell proliferation. Although the specific roles of c-fos and EGF genes in tissue recovery are not known, the expression of these genes may be play an important role in the recovery phase of acute ischemic renal failure.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
