양극성 장애의 약물치료 가이드라인 비교

진서연,김효영,김예슬,허채원,권보영,최보윤,이보배,이지예,권채은,문영도,Fernando Kaveesha,박지현 한국임상약학회 2023 한국임상약학회지 Vol.33 No.3

Objective: Bipolar disorder displays a spectrum of manifestations, including manic, hypomanic, depressive, mixed, psychotic, and atypical episodes, contributing to its chronic nature and association with heightened suicide risk. Creating effective pharmacotherapy guidelines is crucial for managing bipolar disorder and reducing its prevalence. Treatment algorithms grounded in science have improved symptom management, but variations in recommended medications arise from research differences, healthcare policies, and cultural nuances globally. Methods: This study compares Korea’s bipolar disorder treatment algorithm with guidelines from the UK, Australia, and an international association. The aim is to uncover disparities in key recommended medications and their underlying factors. Differences in CYP450 genotypes affecting drug metabolism contribute to distinct recommended medications. Variances also stem from diverse guideline development approaches—expert consensus versus metaanalysis results—forming the primary differences between Korea and other countries. Results: Discrepancies remain in international guidelines relying on meta-analyses due to timing and utilized studies. Drug approval speeds further impact medication selection. However, limited high-quality research results are the main cause of guideline variations, hampering consistent treatment conclusions. Conclusion: Korea’s unique Delphi-based treatment algorithm stands out. To improve evidence-based recommendations, large-scale studies assessing bipolar disorder treatments for the Korean population are necessary. This foundation will ensure future recommendations are rooted in scientific evidence.

대학 예비교수자의 TPACK 역량에 대한 교육요구도 분석

진서연,엄미리,김수인,김효정 고려대학교 교육문제연구소 2021 敎育問題硏究 Vol.34 No.3

The purpose of this study is to derive the educational needs of prospective professors for TPACK (Technology, Pedagogy, and Content Knowledge) competency and prepare a foundation for the design and improvement of educational programs that can assist competency development. To this end, an online survey was conducted for prospective professors at K University in Seoul, using a questionnaire adapted from the TPACK questionnaire. Survey responses from 100 prospective professors were analyzed using the paired t-test, the Borich’s needs assessment model, and The Locus for Focus model. As a result of this study, it was found that among the technology competencies, prospective professors' priorities in educational needs were derived from Technological Knowledge (TK), Content Knowledge (CK), and Pedagogical Knowledge (PK). The result of this study is valuable in that it can be used to set a direction in developing teaching support and educational programs by grasping the TPACK competency and educational needs of prospective professors. 본 연구는 대학 예비교수자의 TPACK 역량에 대한 교육요구를 도출하고 역량개발에 도움을 줄 수 있는 교육프로그램 개발과 개선을 위한 기초자료를 마련하는 데 목적이 있다. 이를 위해 TPACK 설문을 번안한 문항을 활용하여 서울 소재 K대학교의 예비교수자를 대상으로 온라인 설문을 시행하였다. 예비교수자 100명의 설문 응답은 대응표본 t 검정과 Borich 요구도 분석, The Locus for Focus Model을 활용하여 분석하였다. 연구 결과, 예비교수자는 테크놀로지 지식, 내용지식, 교수학적 지식 역량에서 최우선적인 교육요구도가 도출되었으며, 세부적으로는 다양한 종류의 테크놀로지를 알고, 이를 활용하는데 필요한 기술을 가지며, 기술적인 문제를 스스로 해결할 수 있는 능력, 전공과목에 대해 충분한 지식을 가지고 이를 잘 이해하기 위한 다양한 방법과 전략을 가지는 능력, 수업에서 학생의 학습성과를 측정하는 능력, 학생들의 이해도를 측정하는 능력, 학생의 이해도에 따라 다른 수업 방법을 적용하는 능력에 대해 최우선적인 교육을 요구하는 것으로 나타났다. 본 연구의 결과는 대학 예비교수자의 테크놀로지 내용교수지식에 대한 인식과 교육요구도를 파악하여 역량개발에 도움을 줄 수 있는 교육프로그램 개발과 개선을 위한 기초자료로 활용할 수 있다는 점에서 가치가 있다.

비대면 수업환경에서 대학생의 자기주도학습능력에 대한 요구분석

진서연(Seo-Yeon Jin) 고려대학교 교육문제연구소 2023 敎育問題硏究 Vol.36 No.2

대학의 플립드러닝에서 학습 이해도 및 교수·학습자 역할 평가

간진숙, 권명순, 진서연 부산대학교 교육발전연구소 2019 교육혁신연구 Vol.29 No.4

Angiotensin II-Induced Generation of Reactive Oxygen Species Is Regulated by a Phosphatidylinositol 3-Kinase/L-Type Calcium Channel Signaling Pathway

Jin Seo Yeon(진서연),Ha Jung Min(하정민),Kim Young Whan(김영환),Lee Hye Sun(이혜선),Bae Sun Sik(배순식) 한국생명과학회 2015 생명과학회지 Vol.25 No.2

Angiotensin II (AngII)는 혈관평활근세포의 수축을 통해 혈관을 수축시키는 강력한 작용을 나타낼 뿐만 아니라 혈관세포의 성장 등에 중요한 역할을 한다. 본 연구에서는 AngII에 의해 형성되는 활성산소가 phosphatidylinositol 3-kinase (PI3K)에 유리되는 칼슘에 의해 조절된다는 것을 검증하였다. 쥐의 대동맥으로부터 분리된 혈관평활근세포에서 AngII에 의해 활성산소가 농도 의존적, 그리고 시간 의존적으로 형성됨을 관찰하였다. AngII에 의해 형성되는 활성산소는 PI3K의 억제제에 의해 봉쇄되었으나 EKR의 억제제에 의해서는 봉쇄되지 않음을 알 수 있었다. AngII에 의해 유리되는 칼슘은 L-type 칼슘이온통로 봉쇄제인 Nifedipine 또는 배양액에 칼슘이 제거된 환경에서 억제됨을 확인할 수 있었다. 마지막으로 AngII에 의해 형성되는 활성산소는 배양액에 칼슘이 없는 조건이나 L-type 칼슘이온통로 억제제를 전처리 하였을 경우 억제되는 것을 확인하였다. 이러한 결과들을 바탕으로 쥐의 대동맥으로부터 분리된 혈관평활근세포에서 AngII에 의한 활성산소의 형성은 PI3K/L-type 칼슘이온통로를 통한 기전을 통해 조절됨을 제안한다. Angiotensin II (AngII) is an essential hormone that affects vascular physiology. For example, stimulation of vascular smooth muscle cells (VSMCs) rapidly induces vasoconstriction and results in the up-regulation of blood pressure. Chronic stimulation of VSMCs with AngII also results in hypertrophy. In this study, we confirmed an involvement of phosphatidylinositol 3-kinase (PI3K)-dependent calcium mobilization in AngII-induced generation of reactive oxygen species (ROS). Stimulation of rat aortic smooth muscle cells (RASMCs) with AngII significantly induced the generation of ROS in a dose- and time-dependent manner. AngII-induced generation of ROS was completely abolished by pharmacological inhibition of PI3K (with LY294002), but inhibition of the ERK signaling pathway had no effect. AngII-induced calcium mobilization was completely blocked by inhibition of PI3K, whereas inhibition of the ERK signaling pathway by PD98059 was ineffective. Depletion of extracellular calcium or inhibition of the L-type calcium channel by nifedipine completely blocked AngII-induced calcium mobilization. Depletion of extracellular calcium by EGTA and incubation of RASMCs with calcium- free medium both significantly blocked AngII-induced ROS generation. Inhibition of the L-type calcium channel also significantly blocked AngII-induced ROS generation. These results suggest that AngII-induced ROS generation is regulated by calcium mobilization, which, in turn, is modulated by a PI3K/L-type calcium channel signaling pathway.

국내 예비교사 대상 TPACK 향상 교육 프로그램의 효과에 관한 메타분석

정슬기,진서연,박인우 한국교육정보미디어학회 2023 교육정보미디어연구 Vol.29 No.2

As technology integration competencies are emphasized as one of the competencies that pre-service teachers should have, research on TPACK(Technological Pedagogical and Content knowledge) improvement education programs for pre-service teachers have increased. However, the results of individual studies show different effects depending on the research subject and program content. Therefore, this study systemically analyzed and synthesized the effectiveness of TPACK improvement education programs for pre-service teachers conducted in Korea. To this end, 16 studies were selected out of 654 studies published in Korea from 2007 to July 2022, and 41 effect sizes from 16 studies were analyzed. Sub-group analyses were conducted to identify the effect of moderating variables. The results of the study are as follows. First, the overall effect size of TPACK improvement education programs was =0.773, which represents a large effect size. Second, as a result of analyzing the effect size of each dependent variable, teaching expertise, self-efficacy for technology integration and TPACK had large effect sizes. Attitudes toward convergence had medium effect sizes. Third, differences in results moderated by publication types, research participants, subjects, and treatment duration were not significant. Fourth, among the program contents, only reflection had a positive effect on pre-service teachers. This study identified the effectiveness of TPACK improvement education programs and provided preliminary data for developing TPACK improvement education programs. 예비교사가 갖춰야 할 역량 중 하나로 테크놀로지 활용 역량이 강조됨에 따라, 예비교사 대상의 TPACK(테크놀로지 내용교수지식, Technological Pedagogical and Content knowledge, TPACK) 향상 교육 프로그램에 관한 연구가 증가해왔다. 그러나 개별 연구의 결과는 연구대상, TPACK 향상 교육 프로그램의 구성내용에 따라 각기 다른 효과를 나타내고 있으므로, 체계적인 분석을 통해 이질성의 원인을 파악할 필요가 있다. 따라서 본 연구는 국내에서 수행된 예비교사 대상의 TPACK 향상 교육 프로그램의 효과성을 체계적으로 분석하고 종합하였다. 이를 위해 국내 연구가 시작된 2007년부터 2022년 7월까지 발표된 654편의 연구물 중 선정기준에 부합하는 16편의 연구에서 총 41개의 효과크기를 분석하였다. 또한 종속변인의 유형, 자료유형, 연구대상, 교과, 교육내용 등에 따른 집단 간 효과크기의 차이를 분석하였다. 연구 결과는 다음과 같다. 첫째, TPACK 향상 교육 프로그램의 전체 효과크기는 =0.773으로 큰 효과크기가 나타났다. 둘째, 종속변인별 효과크기를 분석한 결과, 교수 전문성, 테크놀로지 통합에 대한 자아효능감, TPACK에서 큰 효과크기가 나타났고, 융합에 대한 태도에서는 중간 정도의 효과크기가 나타났다. 셋째, 자료유형, 연구대상, 교과, 처치기간은 TPACK 향상 교육 프로그램의 효과에 유의한 영향을 미치지 않았다. 넷째, 교육내용 중 성찰을 포함한 TPACK 향상 교육 프로그램이 예비교사에게 긍정적인 효과를 미쳤다. 본 연구는 TPACK 향상 교육 프로그램의 효과크기와 교육내용에 따른 효과크기를 규명하였다. 이는 예비교사 대상 TPACK 향상 교육 프로그램의 가치를 규명하고, 향후 TPACK 향상 교육 프로그램 개발을 위한 기초자료를 제시하였다는 점에서 의의가 있다.

Lysophosphatidic Acid Stimulates SKOV-3 Cell Migration through the Generation of Reactive Oxygen Species via the mTORC2/Akt1/NOX Signaling Axis

김은경,진서연,하정민,배순식 한국생명과학회 2023 생명과학회지 Vol.33 No.2

Reactive oxygen species (ROS) play an essential role in a variety of cellular physiological phenomena. The present study assessed the signaling axis that mediates the lysophosphatidic acid (LPA)-induced migration of SKOV-3 cells. Insulin-like growth factor-1 (IGF-1) stimulated SKOV-3 cell migration in a time- and dose-dependent manner. Similarly, LPA stimulated SKOV-3 cell migration and the phosphorylation of Akt in a time- and dose-dependent manner. The pharmacological inhibition of LPA receptors (LPA1/LPA3) significantly suppressed LPA-induced SKOV-3 cell migration. However, IGF- 1-induced SKOV-3 cell migration was not affected by the inhibition of LPA1 and LPA3. Pharmacological inhibition of phosphoinositide 3-kinase (PI3K) or Rho-associated kinase (ROCK) significantly suppressed LPA-induced migration, whereas the inhibition of MAPK kinase (MEK) had no effect. Inhibition of PI3K or ROCK completely suppressed LPA-induced ROS generation, and suppression of nicotinamide adenine dinucleotide phosphate oxidase (NOX) or chelation of ROS by N-acetylcysteine (NAC) blocked LPA-induced SKOV-3 cell migration. LPA-induced ROS generation was suppressed by silencing Rictor or Akt1 but not Raptor or Akt2. Silencing Rictor or Akt1 significantly suppressed LPA-induced SKOV-3 cell migration, whereas silencing Raptor or Akt2 had no effect. Finally, the overexpression of the constitutively active form Akt1 (CA-Akt1) significantly enhanced the LPA-induced migration of SKOV-3 cells. Given these results, we suggest that LPA stimulates SKOV-3 cell migration by ROS generation, which is mediated by the mTORC2/Akt1/NOX signaling axis.

대학혁신지원사업 교육영역 프로그램 분석 틀 개발 및 유형화 : 수도권 대형사립대학을 중심으로

김효정,진서연 대학교육개발센터협의회 2023 대학 교수-학습 연구 Vol.16 No.2

본 연구는 대학혁신지원사업의 교육영역 프로그램을 분석하기 위한 틀을 마련하고, 프로그램의 유형을 살펴 향후 재정지원사업의 방향성을 제시하는 데 목적이 있다. 이를 위해 대학혁신지원사업 1주기 사업에 참여한 수도권 대형사립대학 중 2개년 이상 평가 A등급을 받은 7개교의 3차년도 사업계획서를 분석하였다. 그 결과, 학사제도, 교육방법, 교육환경, 교육내용, 비교과, 성과관리의 6개 영역과 23개 유형으로 구성된 분석 틀을 개발하였다. 또한, 영역별 프로그램을 유형화하여 교육 프로그램의 구체적인 양상을 탐색하였다. 연구 결과는 교육현장에서 이루어지고 있는 재정지원사업의 현황을 탐색하고, 일관성 있게 비교하는 데 기초 자료를 제공할 수 있을 것이다.

Regulation of retinal angiogenesis by endothelial nitric oxide synthase signaling pathway

하정민,진서연,이혜선,신화경,이동형,송상헌,김치대,배순식 대한약리학회 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.5

Angiogenesis plays an essential role in embryo development, tissue repair, inflammatory diseases, and tumor growth. In the present study, we showed that endothelial nitric oxide synthase (eNOS) regulates retinal angiogenesis. Mice that lack eNOS showed growth retardation, and retinal vessel development was significantly delayed. In addition, the number of tip cells and filopodia length were significantly reduced in mice lacking eNOS. Retinal endothelial cell proliferation was significantly blocked in mice lacking eNOS, and EMG-2-induced endothelial cell sprouting was significantly reduced in aortic vessels isolated from eNOS-deficient mice. Finally, pericyte recruitment to endothelial cells and vascular smooth muscle cell coverage to blood vessels were attenuated in mice lacking eNOS. Taken together, we suggest that the endothelial cell function and blood vessel maturation are regulated by eNOS during retinal angiogenesis.

Regulation of vascular smooth muscle phenotype by crossregulation of krüppel-like factors

하정민,배순식,윤성지,진서연,이혜선,김선자,신화경 대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.1

Regulation of vascular smooth muscle cell (VSMC) phenotype plays an essential role in many cardiovascular diseases. In the present study, we provide evidence that krüppel-like factor 8 (KLF8) is essential for tumor necrosis factor α (TNFα)-induced phenotypic conversion of VSMC obtained from thoracic aorta from 4-week-old SD rats. Stimulation of the contractile phenotype of VSMCs with TNFα significantly reduced the VSMC marker gene expression and KLF8. The gene expression of KLF8 was blocked by TNFα stimulation in an ERK-dependent manner. The promoter region of KLF8 contained putative Sp1, KLF4, and NFκB binding sites. Myocardin significantly enhanced the promoter activity of KLF4 and KLF8. The ectopic expression of KLF4 strongly enhanced the promoter activity of KLF8. Moreover, silencing of Akt1 significantly attenuated the promoter activity of KLF8; conversely, the overexpression of Akt1 significantly enhanced the promoter activity of KLF8. The promoter activity of SMA, SM22α, and KLF8 was significantly elevated in the contractile phenotype of VSMCs. The ectopic expression of KLF8 markedly enhanced the expression of SMA and SM22α concomitant with morphological changes. The overexpression of KLF8 stimulated the promoter activity of SMA. Stimulation of VSMCs with TNFα enhanced the expression of KLF5, and the promoter activity of KLF5 was markedly suppressed by KLF8 ectopic expression. Finally, the overexpression of KLF5 suppressed the promoter activity of SMA and SM22α, thereby reduced the contractility in response to the stimulation of angiotensin II. These results suggest that cross-regulation of KLF family of transcription factors plays an essential role in the VSMC phenotype.