하제로 쓰인 광물성 지방에 의한 지방성 폐렴 1례

조선자,이광민,최진옥,박강서 대한 소아알레르기 및 호흡기학회 2000 소아알레르기 및 호흡기학회지 Vol.10 No.1

Lipoid pneumonia is chronic, interstitial, proliferative inflammation resulting from aspiration of lipoid material. Mineral oil is a hydrocarbon that physicians often use to treat chronic constipation in children and adults. Mineral oil may not elicit a normal protective cough reflex and may impair mucociliary transport. We experienced a case of exogenous lipoid pneumonia caused by aspiration of mineral oil given as a laxatives confirmed by fiberoptic bronchoscopy with bronchoalveolar lavage and bronchial biopsy in a 9-month-old boy with chronic cough and radiologic evidence of parenchymal lung disease. We reported this case with a brief review of related literatures.

Identification and Characterization of 3,6-anhydro-L-galactonate Cycloisomerase Belonging to the Enolase Superfamily

조선자,김정아,이선복 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3

Recently, we have shown that Postechiella marina M091 degrades 3,6-anhydro-L-galactose (L-AnG) to pyruvate and D-glyceraldehyde-3-phosphate via six enzyme-catalyzed reactions and that the L-AnG dehydrogenase, an enzyme catalyzing the first step of L-AnG degradation, converts L-AnG to 3,6-anhydro-L-galactonate (L-AnGA). In this study, we report the identification and characterization of L-AnGA cycloisomerase (L-AnGACI), a novel enzyme that catalyzes the second step of L-AnG metabolism in agar-degrading microorganisms. To characterize this enzyme, the L-AnGACI gene (M091_0722) from P. marina (Pm_LAnGACI) was cloned and expressed in E. coli. The recombinant Pm_L-AnGACI catalyzed conversion of LAnGA to 2-keto-3-deoxy-L-galactonate (L-KDGal), which was confirmed by the L-KDGal aldolase reaction and the LC-MS analysis of the aldolase reaction products. The enzyme showed activity only towards L-AnGA (100%) and galactarate (1.8%) among the 12 sugar acids and carboxylates tested, and the enzyme activity was maximal at 30°C and pH 8.0. Enzyme activity was enhanced by addition of divalent ions such as Co2+ and Mg2+, as is observed from enzymes of the enolase superfamily. LAnGACI conserves several residues commonly found in the enolase superfamily, including three metal-ion binding ligands (Asp198, Glu224, Glu250) and five active-site residues (Lys167, Lys169, Asp273, His300, Glu320). Phylogenetic analysis of amino acid sequences indicates that Pm_L-AnGACI belongs to a novel family within the mandelate racemase subgroup of the enolase superfamily. A reaction mechanism for cycloisomerization of L-AnGA to L-KDGal is proposed, which implies that the absolute configuration does not change during the reaction. To our knowledge, this is the first report on the characterization of L-AnGACI.

Identification and Characterization of 3,6-anhydro-L-galactose Dehydrogenases Belonging to the Aldehyde Dehydrogenase Superfamily from Marine and Soil Microorganisms

조선자,이선복 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.6

The complete hydrolysis of agarose producesits monomeric sugars, D-galactose and 3,6-anhydro-Lgalactose(L-AnG). Although enzymes of D-galactosemetabolism are well characterized, those involved in L-AnGmetabolism have not yet been investigated. In this study,we report the identification and characterization of L-AnGdehydrogenase (L-AnGDH), an aldehyde dehydrogenase(ALDH), catalyzing the first step of L-AnG degradation. To compare substrate and cofactor specificities of L-AnGDH,two L-AnGDH genes obtained from the marine bacteriumPostechiella marina (Pm_L-AnGDH) and the soil bacteriumStreptomyces coelicolor (Sc_L-AnGDH) were cloned andexpressed in E. coli. Whereas the recombinant Pm_LAnGDHand Sc_L-AnGDH were similar in their oligomericstate (homotetramer) and optimum reaction conditions(30°C, pH 8.0), the two enzymes were distinguishable bytheir substrate and cofactor specificities. Sc_L-AnGDHcatalyzed the oxidation of L-AnG using both NAD+ andNADP+, with a preference for NAD+. It also catalyzed thedehydrogenation of L-glyceraldehyde, glycolaldehyde, andL-lactaldehyde in the presence of NAD+. On the otherhand, Pm_L-AnGDH showed exclusive selectivity towardsNADP+ and did not oxidize aldehydes other than L-AnGand L-glyceraldehyde. The phylogenetic analysis of aminosequences indicated that L-AnGDH belongs to a novelsubfamily within the ALDH superfamily. To our knowledge,this is the first report on the characterization of L-AnGDH.

신생아의 유문부 중복성 낭종 1례

조선자,이강호,주명진,이오경 대한소아청소년과학회 2001 Clinical and Experimental Pediatrics (CEP) Vol.44 No.9

주위에서 잘못 전해지고 있는 육아 상식에 관한 연구

조선자,이강호,정영택 대한소아청소년과학회 2002 Clinical and Experimental Pediatrics (CEP) Vol.45 No.1

Purpose : An inexperienced mother depends on the recommendation by an experienced person on how to bring up her child. Therefore, we performed a study to evaluate the wrong methods of childcare and to establish effective childcare counselling. Methods : Two thousand eighty questionnaires filled out by mothers who visited local pediatric clinics and medical centers in Chonbuk, from March 2001 to April 2001, were analyzed according to the distribution of location, age of infant, maternal age and the question of each field. Results : Many mothers have been known to squeeze the breast causing hypertrophy of the neonate. Most infants are placed on their side or supine for sleep. The most common reason for stopping breast feeding was insufficient amount of breast milk. The appropriate age to start whole cow's milk is after the first year of life. The main food used for weaning was commercial baby food. The main oral hydration solution for diarrhea has been boiled water. The main reasons for using a pacifier were to help the mother. The most common reason to use herbs was for the prevention of disease. The use of infant walkers has been widespread among infants and young children. The most common reason was to keep the infant quiet and happy. Most mothers did not use car safety seats for young children. Conclusion : The findings of this study revealed that many mothers were misinformed concerning childcare, so pediatricians should make more efforts and perform more studies to establish rational methods of childcare. 목 적 : 우리나라에서는 아이를 키울 때 경험이 없는 초보 엄마들이 집안어른이나 이웃 사람들에게서 전해들은 육아 상식에 상당히 의존하는 경향이 있는데 그 중에는 아주 현명하고 과학적인 육아 방식도 많이 있으나 간혹 잘못되어 있는 내용도 있어 오히려 질병을 야기하는 경우도 있다. 이에 우리 주위에서 전해지고 있는 육아 방법 중 어떤 것이 잘못 전해지고 있는 내용인지 살펴보고 올바른 방법을 제시하고자 이 연구를 시작하였다.방 법 : 2001년 3월부터 2001년 4월까지 전라북도 지역에 있는 종합병원 및 개인 소아과 의원에 방문한 환아의 어머니를 대상으로 육아 상식에 대한 설문 조사를 시행하였고 회수된 총 2,080부의 설문지를 이용하여 지역별, 아이 연령별, 어머니 연령별 분포 및 각 문항별 질문에 대한 빈도를 조사하였다.

원예활동이 지역아동센터 학생의 정서지능에 미친 영향에 관한 연구

권아현A-hyun,Guan,원옥분Ok-bun,Won,조선자Sun-ja,Cho,제의숙Eu-Sook,Jea 인간식물환경학회 2016 인간식물환경학회 학술대회 Vol.2016 No.추계

발레,한국무용,일반학생간의 유산소 능력 및 체지방율 비교

조선자,김태운 釜山大學校 附設 體育科學硏究所 1991 體育科學硏究所 論文集 Vol.7 No.-

This study is to investigate the physiological influence of dancing on the body by comparision of %Fat and pulmonary function among three groups of college students-Sixteen ballet majoring students(Group A), thirteen Korean dance majors(Group B) and seventeen general stdents(Group C). A total of 46 freshmen and sophomores of P university were tested in view of, the Heart rate at rest, Vital capacity, Vo₂max, Vo₂max/wt, % Fat, Body Density. The results are as follows. 1. The heart rate at rest of Group A(69.9±6.8beats/min)and those of Group B(72.9±6.6beats/min) showed significantly lower statistics than those of Group C(80.2±9.4beats/min) 2. Vital capacity of Group A(3270.5±261.3㏄) and Group B(3048.3±310.9㏄) showed significantly higher statistics than those of group C(3025.9±312.7㏄) 3. Vo₂max was the highest among Group A(1.96±0.27ℓ/min), the second highest among Group B(1.78±0.27ℓ/min) and the lowest among Group C(1.74±0.15ℓ/min) 4. Vo₂max/wt of Group A and Group B was 38.00±5.92㎖/㎏/min(the highest) and 36.58±6.28㎖/㎏/min respectively. Vo₂max/wt of Group A showed meaningful difference from that of Group C.(32.61±5.31㎖/㎏/min) 5. % Fat of Group A and Group B was 20.30±3.57 %(the lowest), and 20.53±4.90%, respectively, which showed difference from that of Group C(23.63±3.09%) 6. Body density of Group A(1.0519±0.0086g/㎖) and Group B(1.0513±0.0117g/㎖) was meaningful higher than that of group C(1.0438±0.0117g/㎖)

초등 실과 교육과정에 기반한 진로교육 프로그램이 진로인식에 미치는 영향

김유정,조선자 한국실과교육연구학회 2010 實科敎育硏究 Vol.16 No.4

본 연구의 목적은 초등 실과 교육과정에 기반한 진로교육 프로그램이 초등학생의 진로인식에 미치는 영향을 검증하는데 있다. 이를 위해 실과 교육과정에 기반한 진로교육 프로그램을 개발하여 초등학교 5학년 실과시간에 주 1회 연차시로 80분씩 총 24차시에 걸쳐 진로교육 프로그램을 실시한 후 진로인식의 변화 정도를 살펴보았으며, 수집된 자료의 분석은 SPSSWIN 12.0 프로그램을 활용하였다. 이 연구를 통하여 얻은 결과는 다음과 같다. 첫째, 진로교육프로그램을 개발하기 위해 실과교과에서 단원별, 차시별로 진로교육 관련 지도요소를 선정하고 지도 요소에 따라 동기유발자료 및 활동자료로 16개의 프로그램을 개발하였으며, 단원 마지막 차시에는 단원과 관련 있는 직업을 탐색하여 직업사전 만들기 활동을 하였다. 둘째, 본 연구에서 개발한 실과 교육과정에 기반한 진로교육 프로그램의 효과를 검증한 결과, 초등학생의 진로인식에 긍정적인 영향을 주는 것으로 나타났다. 셋째, 실과 교육과정에 기반한 진로교육 프로그램은 진로인식의 하위 요소인 아동의 자아인식 능력, 일의 직업의 세계 능력, 일에 대한 긍정적 태도 능력, 의사결정능력 향상에 긍정적으로 기여하였음이 검증되었다. The purpose of this study was to verify the effect of career education program in practical course on the elementary students' recognition of career. The research contents set up according to such purpose are ; First, to develop the career education program that can improve elementary students' recognition of career ; Second, to analyze the effect of elementary students' recognition of career by applying the career education program. In the career education program developed by this research, career education program-related teaching elements were selected from the practical course by chapter and lesson, and 16 programs including motivation sources and activity sources were developed according to the teaching elements. At the last lesson of a chapter, vocation that is related the chapter was searched to make a vocational dictionary. The career recognition test was administered to both control and comparison group to verify the effect of career education program in practical course on the elementary students' recognition of career. Collected data were analyzed using SPSSWIN 12.0 and t-test and paired-t test were carried out to verify the content of research. Following is the conclusion of this study. First, the verification of both control and comparison group that went through the career education program of practical course showed a significant difference suggesting that the career education program had a positive effect on elementary students' recognition of career. Second, it was proved that the practice of career education program in practical course contributed positively to the sub-elements of career recognition, children's self-recognition ability, vocational ability, positive attitude ability toward job and improvement of decision-making ability. Therefore, it was proved that career education program in elementary practical course subject had a very positive effect on elementary students' career recognition.

용서교육프로그램이 모(母) 불안정 애착아동의 용서수준과 모(母)애착수준에 미치는 효과

김남순,조선자 한국교육학회 2007 敎育學硏究 Vol.45 No.4

The purpose of this study was to examine effects of a forgiveness education program on the level of forgiveness and attachment level in elementary school children who had formed mother insecure attachment. As a result, first, the experimental group showed a higher mean in the level of forgiveness and its sub-areas than the control group, but showing a significant increase only in the behavioral area; there was a significant increase in the level of forgiveness within the experimental group. Second, the experimental group showed a higher mean in the level of mother attachment and its sub-areas, but showing a significant increase only in the communication area; there was statistically significant difference in results within the experimental group. With the process of the program, positive changes could be found in children through children's writings about their impression on activities and teachers' observations. It was therefore demonstrated that a forgiveness education program was partially effective in improving level of mother attachment and the level of forgiveness in children with insecure attachment. 본 연구는 용서교육프로그램이 모(母) 불안정 애착을 형성한 초등학생들의 용서수준과 모(母) 애착 수준에 미치는 효과를 밝혀보고자 하였다. 연구대상은 M초등학교 5학년 학생 중 모(母) 불안정 애착아동으로 선정된 30명이며 그들은 각각 실험집단과 통제집단으로 구성되었다. 이 가운데 실험집단만 용서교육프로그램을 4주간 실시하였으며, 통제집단에는 아무런 처치도 가하지 않았다. 실험 개시 전 두 집단 모두 사전검사를 하고 실험이 끝난 후 사후검사를 실시하였다. 연구 결과 첫째, 용서교육프로그램을 실시한 실험집단은 통제집단에 비해 전체 용서수준, 인지적 및 정서적 영역에서는 유의미한 차이를 보이지 않았으나 용서수준의 하위영역 중 행동적 영역만 유의미한 증가를 보였다. 둘째, 실험집단은 통제집단에 비해 전체 모(母) 애착수준, 신뢰감 및 소외감 영역에서는 유의미한 차이를 보이지 않았으나 모 애착척도의 하위영역 중 의사소통영역만 유의미한 증가를 보였다. 또한 질적 분석 결과 프로그램 과정이 진행됨에 따라 아동들 대부분은 긍정적인 변화를 보였으나 일부 아동들은 기대한 만큼의 효과를 보여주지 못하였다. 이와 같은 연구결과로 볼 때 용서교육프로그램은 불안정 애착아동의 용서수준과 모(母) 애착 수준을 긍정적으로 변화시키는데 부분적인 효과가 있음을 입증하였다.

Metabolic Pathway of 3,6-anhydro-L-galactose in Agar-degrading Microorganisms

이선복,조선자,김정아,이신엽,김석민,임현승 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.5

Recently, agarose-containing macroalgae havegained attention as possible renewable sources for bioethanolproduction because of their high polysaccharide content. Complete hydrolysis of agarose produces two monomers,D-galactose (D-Gal) and 3,6-anhydro-L-galactose (L-AnG). However, at present, bioethanol yield from agarophytemacroalgae is low due to the inability of bioethanolproducingmicroorganisms to convert non-fermentable sugars,such as L-AnG, to bioethanol. Therefore, to increase thebioethanol productivity of agarophytes, it is necessary todetermine how agar-degrading microorganisms metabolizeL-AnG, and accordingly, construct recombinant microorganismsthat can utilize both D-Gal and L-AnG. Previously, we isolated a novel microorganism belongingto a new genus, Postechiella marina M091, which hydrolyzesand metabolizes agar as the carbon and energy source. Here, we report a comparative genomic analysis of P. marinaM091, Pseudoalteromonas atlantica T6c, and Streptomycescoelicolor A3(2), of the classes Flavobacteria, Gammaproteobacteria,and Actinobacteria, respectively. In thisbioinformatic analysis of these agarolytic bacteria, wefound candidate common genes that were believed to beinvolved in L-AnG metabolism. We then experimentallyconfirmed the enzymatic function of each gene product in theL-AnG cluster. The formation of two key intermediates,2-keto-3-deoxy-L-galactonate and 2-keto-3-deoxy-D-gluconate,was also verified using enzymes that utilize these moleculesas substrates. Combining bioinformatic analysis andexperimental data, we showed that L-AnG is metabolized topyruvate and D-glyceraldehyde-3-phosphate via six enzymecatalyzedreactions in the following reaction sequence:3,6-anhydro-L-galactose → 3,6-anhydro-L-galactonate →2-keto-3-deoxy-L-galactonate → 2,5-diketo-3-deoxy-Lgalactonate→ 2-keto-3-deoxy-D-gluconate → 2-keto-3-deoxy-6-phospho-D-gluconate → pyruvate + D-glyceraldehyde-3-phosphate. To our knowledge, this is the first report on themetabolic pathway of L-AnG degradation.