대장균의 저온 충격단백질에 관한 연구

조명선,정태진,김남덕,정재성,정재훈,이영훈,Cho, Myung-Sun,Cheong, Tae-Chin,Kim, Nam-Deok,Jung, Jae-Sung,Chung, Jae-Hoon,Lee, Young-Hoon 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.6

정상적인 온도에서 생장하고 있는 박테리아 배양액을 저온으로 옮겨 주었을 때 생성이 증가되는 단백질의 양상을 1차원 및 2차원 전기영동에 의해 조사하였다. 이러한 저온 충격단백질들의 생성은 충격을 받은 온도에 따라 다소 다르게 나타났으며 일단 생성된 저온 충격단백질들은 $37^{\circ}C$ 로 다시 온도를 옮겼을 때에도 안정한 것으로 관찰되었다. 저온 충격단백질의 생성기작을 이해하기 위해 저온 충격단백질로 알려진 RecA의 유전자를 pUC19에 클로닝한 후 재조합 플라스미드를 함유하는 군주들에서 저온 충격에 의해 RecA 단백질의 생성이 증가됨을 분자수준에서 증명하였다. The cold shock proteins induced by temperature shift from $37^{\circ}C$ to $10^{\circ}C$ were assayed by the two-dimensional polyacrylamide gel electrophoresis after the proteins were pulse-labeled with $^{35}S$-methionine. The cold shock proteins were quite stable even if the temperature was returned to $37^{\circ}C$. The cloned recA gene was used to understand the molecular mechanism of the cold shock response. Synthesis of RecA protein from the recA gene harbored on the multicopy plasmid was increased upon cold shock, suggesting that the cold shock response involves the regulation by unlimited cellular factors such as RNA polymerase.

저온 충격유전자 recA의 발현 증가기작에 관한 연구

조명선,정태진,정재성,이영훈,조철오,정재훈,Cho, Myung-Sun,Cheong, Tae-Chin,Jung, Jae-Sung,Lee, Young-Hoon,Joe, Cheol-O,Chung, Jae-Hoon 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.8

저온 충격단백질의 생성기작을 분자수준에서 이해하고자 저온 충격단백질의 하나로 알려진 RecA를 선택하여 이것의 저온 충격에 의한 생성 증가기작을 연구하였다. Northern 브럿에 의한 전사체 양의 비교 및 융합된 CAT(크로람페니콜 아세틸기 전달효소) 유전자의 발현을 통하여 저온 충격에 의해 recA 유전자의 발현이 2배 증가되는 것이 관찰되었다. 이러한 결과로 미루어 저온 충격 대응기작이 전사단계에서 이루어짐을 알수 있었다. 또한 저온 충격에 관여하는 조절인자의 존재여부를 조사하기 위하여 recA 유전자의 촉진유전자 부위에 결합하는 단백질에 대한 실험결과 recA 유전자의 촉진유전자에 조절인자가 결함하는 것이 저온 충격에 의해 오히려 억제되는 것으로 나타났다. Temperature downshift from $37^{\circ}C$ to $10^{\circ}C$ or $18^{\circ}C$ induces over 10 cold shock proteins in Escherichia coli. We have reported that the synthesis of RecA, one of the cold shock proteins, was increased two-fold upon cold shock when it was expressed in multicopy plasmid. In an attempt to understand the molecular mechanism of cold shock response, the expression of the recA gene was examined at transcriptional level in this study. Northern blotting and chloramphenicol acetyl transferase activity from the recA-CAT fusion plasmid revealed that the recA transcription was increased 2~3 fold by cold shock, indicating that the cold shock response is at the level of transcription. The gel mobility shift experiment suggested that cold shock might induce the transcription by releasing the regulatory factor(s) from the recA promoter region.

대장균의 저온 충격단백질에 관한 연구

조명선,정태진,김남덕,정재성,정재훈,이영훈 ( Myung Sun Cho,Tae Chin Cheong,Nam Deok Kim,Jae Sung Jung,Jae Hoon Chung,Young Hoon Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.6

The cold shock proteins induced by temperature shift from 37℃ to 10℃ were assayed by the two-dimensional polyacrylamide gel electrophoresis after the proteins were pulse-labeled with ^(35)S-methionine. The cold shock proteins were quite stable even if the temperature was returned to 37℃. The cloned recA gene was used to understand the molecular mechanism of the cold shock response. Synthesis of RecA protein from the recA gene harbored on the multicopy plasmid was increased upon cold shock, suggesting that the cold shock response involves the regulation by unlimited cellular factors such as RNA polymerase.

저온 충격유전자 recA의 발현 증가기작에 관한 연구

조명선,정태진,정재성,이영훈,조철오,정재훈 ( Myung Sun Cho,Tae Chin Cheong,Jae Sung Jung,Young Hoon Lee,Cheol O Joe,Jae Hoon Chung ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.8

Temperature downshift from 37℃ to 10℃ or 18℃ induces over 10 cold shock proteins in Escherichia coli. We have reported that the synthesis of RecA, one of the cold shock proteins, was increased two-fold upon cold shock when it was expressed in multicopy plasmid. In an attempt to understand the molecular mechanism of cold shock response, the expression of the recA gene was examined at transcriptional level in this study. Northern blotting and chloramphenicol acetyl transferase activity from the recA-CAT fusion plasmid revealed that the recA transcription was increased 2∼3 fold by cold shock, indicating that the cold shock response is at the level of transcription. The gel mobility shift experiment suggested that cold shock might induce the transcription by releasing the regulatory factor(s) from the recA promoter region.

지역사회에 있는 정신장애인의 희망과 회복: 치료자와의 치료적 관계의 매개효과

현명선 ( Myung Sun Hyun ),박은영 ( Eun Young Park ),김영희 ( Young Hee Kim ),김유라 ( Yu Ra Kim ),조명선 ( Myung Sun Cho ) 한국간호과학회 정신간호학회(구 대한간호학회정신간호학회) 2014 정신간호학회지 Vol.23 No.3

Purpose: This study was conducted to examine the association among therapeutic relationship with mental health professionals, hope, and recovery, and to explore the mediation of therapeutic relationship with mental health professionals in the relation between hope and recovery in South Korean patients with a mental illness. Methods: A cross-sectional study design was employed. Eligible participants were 99 people with a mental illness who were enrolled as members at two Community Mental Health Centers in Gyeonggi Province. Data were collected from October, 4 to 14, 2011. Data were analyzed using descriptive statistics, Pearson correlation coefficients, and multiple regression analysis. Results: Hope was positively correlated with recovery, and therapeutic relationship with mental health professionals was also positively associated with recovery. The mediating effect of therapeutic relationship with mental health professionals was found in the relation between hope and recovery. Conclusion: The results suggest that therapeutic relationship with mental health professionals plays an important role in promoting recovery of the patients with a mental illness. Therefore, mental health professionals should focus on developing a therapeutic relationship with their clients and also consider these recovery-related factors for persons who have a mental illness.

트리테르페노이드의 코르티코이드 유사활성물질에 관한 연구 ( 2 ) 11 - 옥소올레아놀산 및 유도체의 합성과 그 항염증 활성

한병훈,조명선,한용남,박명환 ( Byung Hoon Han,Myung Sun Kim - Cho,Yong Nam Han,Myung Hwan Park ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.1

The effect of 11-oxo-oleanlic acid [I] and its derivatives on carrageenin-induced edema in rat kind paw was investigated. The following derivatives were synthesized ; 11-oxo-oleanolic acid methylester [II] C_(31)H_(48)O₄ mp 196-198° was derived from [I] by methylation with CH₂N₂. 3-Oxo-oleanolic acid [III] C_(30)H_(46)O₃, mP 198-202° was obtained from oleanolic acid by CrO₃-pyridine oxidation. Oxidation of [III] by CrO₃-HAc produced 3,11-dioxooleanolic acid [IV] C_(30)H_(46)O₃, mp 268-270° VU (λ max) 252 ㎚ and 3,11-dioxo-oleanolic acid lactone [V] C_(30)H_(44)O₄, mp 265-267° as a by-product. [IV] showed strong competitive inhibition against corticoid-5β-reductase, K_i= 4. 2×10-qM, compared to 4. 6×10^(-4)M of [I]. The anti-inflammatory activities of 11-oxo-oleanolic acid derivatives were evaluated by rat kind paw carrageenin edema test after s.c, injection with 30㎎/㎏ doses. [I], [II], [IVY and [V] suppressed significantly the edema volumes by 63, 78, 70 and 66%, respectively, compared to 66% of hydrocortisone. The above results suggest that the replacement of OH group in C₃ position by ketone group increased the corticomimetic activity, but that the change on the steric structure in C and D rings by lactone formation reduced the activity increase. [II] showed the most powerful activities probably due to increased lipophilicity.

일개 시 지역의 정신건강 인식에 대한 10년 전후 비교 분석

전우혁(Woo-Hyeok Jeon),조명선(Myung Sun Cho),전덕인(Duk-In Jon),홍현주(Hyun Ju Hong),홍나래(Narei Hong),정명훈(Myung Hun Jung) 대한사회정신의학회 2020 사회정신의학 Vol.25 No.2

연구목적 : 정신질환에 대한 적절한 관리의 필요성이 점점 증가하는 한편 이에 대한 사회적인 인식과 태도는 여전히 부정적인 것이 현실이다. 본 연구는 각각 2008년과 2018년에 조사한 안양 시민의 정신건강에 대한 인식과 정신건강 수준을 비교 평가하고자 한다. 방 법 : 안양시에 거주 중인 20세 이상의 성인 남녀 600여 명을 대상으로 설문조사 하였다. 설문 내용은 사회 인구학적 요인과 함께 정신 건강 종합서비스 욕구, 정신건강 문제 해결을 위한 질문, 연령에 따른 대상자 별 정신건강 서비스 욕구에 관한 질문, 자살 사고에 대한 인식도 및 경험 유무 등으로 이루어졌다. 결 과 : 안양시민은 2008년에 비해 2018년에 스스로의 정신건강 점수를 더 낮게 평가하였으며, 정신건강에 대한 도움의 필요성을 인식하는 비율 및 실제 도움을 받은 비율 또한 감소하였다. 연령별로는 청소년과 노인에서 정신건강 문제의 중요도가 증가하였으며 연령 전반에서는 ‘정서’ 문제의 중요도가 크게 상승하였다. 한편 자살에 대한 인식에 개선이 뚜렷하지 않았고 자살 위기 시 정신건강의 도움에 대한 필요성은 낮게 평가되었다. 결 론 : 일반 시민들이 정신건강에 대한 도움을 부정적으로 인식하는 것은 정신질환 자체에 대한 편견과 부정적인 인식에 의한 것으로 추론된다. 이와 같은 정신건강에 대한 인식 및 편견 해소와 정신건강 서비스 이용을 향상시키기 위하여 다각도의 노력이 필요할 것이다. 또한 청소년 및 노인의 정신건강 문제가 중요하게 보고되며 시민들의 자살 문제는 여전히 개선되지 않아 해당 연령층 및 위험 군을 대상으로 한 세분화된 개입과 지원이 필요할 것이다. Objectives : While the necessity of proper management for mental illness increases gradually, the attitude toward mental health and psychiatric treatment is still remaining negative. The purpose of this study was to compare Anyang citizens’ aware-ness and level of mental health surveyed in 2008 and 2018, respectively. Methods : About six hundred men and women over 20 years old residing at Anyang city were recruited for this study. The selfreport questionnaire consists of the sociodemographic data, needs for overall mental health service, methods to solve their own mental health problem, age-specific needs for the mental health service, and awareness and experience of sui-cidal ideation. Results : Anyang citizens rated their mental health score lower in 2018 than in 2008, and the rate of the need for help to mental health as well as the rate of getting actual help were decreased. Age-specifically, there were large increases in the impor-tance of adolescent and senior citizens’ mental health problem. In all ages, there was a large increase in the importance of emotional problem. Meanwhile, there is no clear improvement in the perception of suicide, and the need for help to mental health at suicidal crisis was estimated low. Conclusion : It is inferred that a negative awareness of the help to mental health is due to negative perception and prejudice toward mental illness itself. Therefore we suggest that multifarious efforts are needed to reduce citizens’ negative perception and prejudice toward mental health as well as to make use of the mental health services. Furthermore, as adolescent and se-nior citizens’ mental health problem were reported importantly, as well as suicide problem is still unimproved, specific intervention and support targeting the relevant groups are necessary.

고등학생의 인터넷 중독경향과 기질특성의 관계

진범수(Bum-Su Chin),정은영(Eun-Young Chung),백경아(Kyung-Ah Baek),조하나(Ha-Na Cho),조명선(Myung-Sun Cho) 한국중독정신의학회 2008 중독정신의학 Vol.12 No.2

Objectives:The purpose of this study was to investigate the relationship between internet addiction tendency and temperament characteristics in high school students. Method:Participants were 280 high school students who resided in Anyang (male/female=148/132). The participants completed a standard questionnaire related to internet use patterns, the Temperament and Character Inventory (TCI), the Korea Internet Addiction Scale (KIAS), and the Beck Depression Inventory (BDI). The subjects were classified into either a risk group or a non-risk group according to their KIAS scores. Results:There was no difference between male and female students in internet addiction tendencies. Compared to the non-risk group, the risk group showed significantly higher ‘novelty seeking’ and ‘harm avoidance’, lower ‘reward dependence’ and ‘persistence’, and a higher BDI score. Persistence was positively correlated with tolerance in the KIAS subscales. Conclusion:This study suggests that high school students with internet addiction tendencies have specific temperament characteristics.

Serratia marcescens 의 R plasmid 로부터 gentamicin 저항성을 갖는 유전자의 cloning

신창호,김인규,정재훈,최영길,연승우,조명선,정재성 한국유전학회 1991 Genes & Genomics Vol.13 No.3

Gentamicin resistance gene residing in Serratia marcescens R plasmid pIS199G2 was cloned after it was transferred to Escherichia coli ML1410 by conjugation. Partial digests of pIS199G2 with Sau3A1 were cloned into the BamHI site of pUC9. One plasmid pSY1 carried 1.5kb insert and showed gentamicin resistant phenotype. A polypeptide product of 31kd was identified from pSY1 in minicell expression system. During the cloning procedure a plasmid narboring a replication origin in R plasmid, ampicillin resistance gene and gentamicin resistance gene was obtained. The plasmid, pSW1, also produced the polypeptide of the same size by the minicell expression system. The restriction enzyme mapping of pSY1 and pSW1 showed homology to other aacC2 type of gentamicin resistance gene and suggests that the two plasmids contain aacC2 gene family.