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코로나19 발생 전후 서울시 동남권 생활인구 수의 변화에 미친 영향 요인 - 슈퍼블록 단위의 분석 -
조동재,황정현,김신,조항훈,김흥순 국토지리학회 2023 국토지리학회지 Vol.57 No.4
This study analyzed the correlation between urban characteristics within superblocks and the living population. The temporal scope covered two years, 2019 before the outbreak of COVID-19 and 2021 after the outbreak. The spatial scope was the southeastern region of Seoul divided into superblocks, and spatial regression analysis was conducted. The analysis results indicated that among the road infrastructure factors, road density showed a positive correlation with the living population in all time periods in 2019 and 2021. Also, except for weekdays from 19:00 to 24:00 in 2019, the crosswalk density showed a positive correlation with the living population in all time periods in both years. Among the building factors, residential density showed a negative correlation with the living population from 07:00 to 19:00 on weekdays in 2019, while business density showed a positive correlation with the living population in both years from 07:00 to 19:00. In terms of public transportation factors, the subway station area ratio was positively correlated with the living population in all time periods for both years.
김민정,김지영,이승재,윤용달,조동재,김해권 한국발생생물학회 2001 발생과 생식 Vol.5 No.1
포유류의 난자가 수란관내로 배란될 때는 난포액 성분도 같이 수란관내로 들어간다. 본 연구에서는 처음으로 난포액의 일부 성분이 수란관액에 의해서 변화하는 것을 관찰하였다. 사람의 난포액을 gelatin zymogram으로 분석한 결과 621kDa gelatinase 이외에 110kDa gelatinase (GA110) 등의 여러 gelatinase 활성이 나타났다. 이 활성들은 EDTA나 phenanthroline에 의해 억제된 반면 PMSF 처리에 의해 When mammalian oocytes ovulate into the oviduct, associating follicular fluid components are exposed to the oviductal environment, possibly resulting in the mutual interaction between fillicu1ar and oviductal fluids. In the Present study, we have demonstrated for the first time that components of fallicular fluid could be modified by the oviductal fluid. Gelatin zymographic analyses of human follicular fluid (hFF) obtained from IVF patients showed consistently the presence of 110 kDa gelatinase (GA110) in addition to many bands among which 62 kDa gelatinase was predominant. Addition of EDTA or phenanfhroline to the gelatinase substrate buffer during gel incubation abolished GA110 band whereas phenylmethylsulffnyl fluoride (PMSF) did not. In contrast, bovine oviductal fluid(bOF) exhibited only 62 kDa gelatinase. Surprisingly, when bOF was added to hFF in 1:1 ratio and then the mixture was incubated for 3 h at 37C, GA110 of hFF disappeared. Disappearance of GA110 by bOF was observed even within 30 min after mixing with hFF. Addition of aminophenylmercuric acetate (APMA) to hFF also abolished enzymatic activity of GA110 but increased the activityof 62 kDa gelatinase. However, APMA abolished many other gelatinases as well unlike bOF. Interestingly, treatment of hFF with EDTA for 3 h remarkably increased the enzymatic activity of GA110 but not that of other gelatinases. Addition of phenanthroline, PMSF or soybean trypsin inhibitor (SBTI) did not affect overall gelatinase activities. Again, addition of bOF to the hFF pretreated with any of the above proteinase inhibitors abolished the appearance of GA110. Human serum also showed GAI 10 of which activity was greatlyenhanced by EDTA treatment. Similar to hFF, serum GA110 also disappeared by the addition of bOF. Human granulosa cell homogenate did not reveal any appreciable gelatinase activity except 92 kDa gelatinase. Anti-human gelatinase A antibody reacted with 62 kDa gelatinase of hFF. Based upon these results, it is concluded that bOF could selectively degrade an isoform of gelatinase A present in hFF and human serum.