뇌혈관장애에서 인지유발전위검사의 유용성

정흥채(Heung-Chae Chung),전진숙(Jin-Sook Cheon),이경민(Kyung-Min Lee),정호중(Ho-Joong Jung),오병훈(Byoung-Hoon Oh) 대한노인정신의학회 2001 노인정신의학 Vol.5 No.2

Objectives:The purpose of this study is to evaluate the clinical applicability of cognitive evoked potentials (CEPs) to identify the cognitive disorder in cerebrovascular diseases (CVD). Methods:The P3 latencies, amplitudes and latency to amplitude ratios (LARs) of CEPs were measured in 25 healthy controls and 35 patients with CVD. The association of CEPs with variables including age, sex, Mini-Mental State Examination (MMSE), CVD types, loci of hemiplegic limbs, duration, education, Brief Psychiatric Rating Scale (BPRS), and Instrumental Activities of Daily Living (IADL) were also analyzed. Results:1) The P3 latencies (447.87±113.06 msec) and LARs (65.83±43.25) were prolonged in CVD (p<0.01), while the amplitudes (8.18±2.51 μV) were not changed. 2) The P3 latencies (537.31±101.14 msec) and LARs (94.89±46.44) in CVD with MMSE <24 were prolonged, and the amplitudes (6.45±1.98 μV) were reduced significantly (p<0.01, respectively), as compared with those in CVD with MMSE≥24 (363.39±19.67 msec, 38.40± 8.37 and 9.81±1.76 μV, respectively). 3) The BPRS and IADL in CVD with MMSE<24 were significantly (p<0.01) different from those in CVD with MMSE≥24. 4) On analyzing the association of CEPs with variables in CVD, the P3 latencies were correlated positively with age, BPRS and IADL, while negatively with MMSE and DLFA. The amplitudes were correlated positively with MMSE, while negatively with age, BPRS and IADL. The LARs were correlated positively with age, BPRS and IADL, while negatively with MMSE. 5) On analyzing the association of CEPs with variables in CVD with MMSE≥24, the P3 latencies were correlated positively with age, while negatively with MMSE. The amplitudes and LARs were not correlated with variables. 6) On analyzing the association of CEPs with variables in CVD with MMSE<24, the P3 latencies were correlated positively with BPRS, while negatively with MMSE. The amplitudes were positively correlated with age. The LARs were positively correlated with IADL. Conclusions:The P3 latencies and LARs of cognitive evoked potentials seemed to a useful clinical measures to assess cognitive disorders in CVD.

MR 댐퍼를 이용한 철도차량 승차감 반능동 제어

신유정(Yu-Jeong Shin),유원희(Won-Hee You),정흥채(Heung-Chae Jung) 한국철도학회 2011 한국철도학회 학술발표대회논문집 Vol.2011 No.10

Recently the maximum speed becomes the most important performance in high speed train. But the speed up of train will not give the passenger good riding comfort. The semi-active suspension system by using variable damper with hydraulic solenoid valve is used to solve this problem. But the variable damper with hydraulic solenoid valve requires tank for supplying fluid. In this study the MR(Magneto Rheological) damper was considered instead of hydraulic variable damper in order to improve riding comfort. Dynamic simulation was conducted for semi-active suspension system with MR damper was made by using Matlab-Simulink S/W. According to control strategy of MR damper for improving ride comfort in railway vehicle The riding comfort of the railway vehicle with semi-active suspension system was analyzed and compared with conventional suspension system by using the program.

고초균 포자를 이용한 Zymomonas mobilis 유래의 levansucrase 표면 발현

김준형(June-Hyung Kim),최수근(Soo-Keun Choi),정흥채(Heung-Chae Jung),반재구(Jae-Gu Pan),김병기(Byung-Gee Kim) 한국생물공학회 2011 KSBB Journal Vol.26 No.3

Using Bacillus subtilis spore display system, with cotG as an anchoring motif, levansucrase from Zymomonas mobilis, was displayed on the outer surface of Bacillus subtilis spore. Flow cytometry of DB104 (pSDJH-cotG-levU) spore, proved the surface localization of CotG-LevU fusion protein on the spore compared to that of DB104. Enzymatic activity of DB104 (pSDJH-cotG-levU) spore showed more than 1.5 times higher levansucrase specific activity compared to that of the host spore, which is a remarkable increase of enzymatic activity considering the existence of sacA (sucrase) and sacB (levansucrase) in the Bacillus subtilis chromosome. The spore integrity, revealed by sporulation frequency test after heat and lysozyme treatment of spore, did not changed at all in spite of the CotG-LevU fusion protein incorporation into the spore coat layer during spore formation process. These data prove again that Bacillus subtilis spore could be considered as good live immobilization vehicle for efficient bioconversion process.

실리콘 튜브를 이용한 동물세포 배양장치의 산소전달

정흥채,김정회 한국산업미생물학회 1992 한국미생물·생명공학회지 Vol.20 No.4

동물세포 배양기에서 silicone tube를 oxygenator로 이용하여 산소전달 정도를 조사하였다. Silicone tube를 이용할 때 표면통기에 비해 산소전달 상수 k_La는 약 30배 이상 증가하였다. 통기속도와 교반속도가 증가할수록 k_La값도 증가하지만 교반속도가 휠씬 효과적이었고, 물질전달에 관한 무차원 상수 tubing Sherwood number(Sh)와 유체 흐름에 관한 상수 impeller Reynolds number(Re)는 log-log 좌표에서 직선관계가 있었고 기울기는 tube 길이에 관계없이 0.26이었다. Tube 길이는 2m가 적당하였고 impeller는 pitched blade type이 효과적이었다. 5% serum이 첨가된 medium에서는 k_La가 40%로 감소하였다. HepG2를 이용한 실제 동물세포 배양에서는 전형적인 세포농도인 4∼6×10^6 cells/㎖를 얻을 수 있었다. An enhancement of the oxygen transfer rate in a 1ℓ bioreactor for mammalian cell culture by using a silicone rubber tubing as an oxygenator was investigated. When the silicone membrane was used to supply oxygen to the culture broth, the oxygen transfer coefficients (k_La) measured in deionized-distilled water were markedly increased. Effect of surface aeration without the tubing aeration was very low under 1.0 hr^-1 of k_La. The enhancing effects of agitation rates on k_La were much more effective than those of aeration rates. The increase of k_La with increasing tube length was observed as a result of the large surface area for oxygen supply. However, 2m of the tube length was adequate for a 1ℓ vessel. The larger blade type of impeller was effective to enhance the k_La values because of its high mixing intensity. In culture medium supplemented with 5% serum, k_La values were reduced to approximately 40% probably due to the viscosity. We also obtained the normal cell concentration of 5×10^6 cells/㎖ of HepG2 on microcarriers, which could be achieved in a typical bioreactor for animal cell culture.

한국에서 정상 및 치매 노인의 성 문제

전진숙,정흥채,송혜란,오병훈,김인성,김현수,조웅,윤한철 대한신경정신의학회 2004 신경정신의학 Vol.43 No.4

Objectives : The aging process can influence sexual functions by physiological, Pathological, behavioral and psychosocial changes. Studies on sex among elderly are scarce. However, sexual activities remain throughout life in different forms. The aim of this study was to identify sexual problems and dysfunctions among dementia patients and compare with healthy aged persons. Methods : The sexual problems and affecting factors were evaluated by structured interview and sexual problem screening instruments for 46 dementia patients according to DSM-IV and 60 healthy Koreans aged over 60. Results : 1) Frequency of erection was less than once a day in 68.0% of controls, while more than once a day in 47.6% of dementia. Trouble in attaining erection was found in 50.0% of dementia as compared with 65.4% of controls. Awake with erection was less than once a week in 37.8% of controls, while less than once a week in 22.2% and more than once a week in 10.0% of dementia. Maintaining erection was better in controls (92.0%) as compared with dementia (55.0%) (p<0.01). Number of sex partner was one in 75.0% and none in 25.0% of controls, while one in 80.0%, none in 16.0% and more than one in 4.0% of dementia. Patn during with intercourse was less in dementia (22.7%) than controls (73.2%) (p<0.001). Frequency of ejaculation and masturbation, frequency of intercourse, thoughts and dreams of sex, feeling of desire was higher in dementia than controls (p<0.05). In 41.7% of dementia patients, sexual activity cf. sexual desire was 1ess(desired>activity). Satisfaction in sex life (41.7%), Partner's satisfaction (31.8%), satisfaction with partner (45.5%) and men's interest in sex decline with age (54.5%) were higher in dementia (p<0.001, respectively). 2) The recognition of sexual problems was higher in healthy elderly (74.1%). Most of sexual dysfunctions except sexual desire disorder (83.3% in controls vs 40.0% in dementia) was higher in demetia, that is, erectile disorder (30.0%), Premature ejaculation (20.0%), and orgasmic disorder (10.0%). The duration over 5 years of sexual problems was the most in both (46.7% in controls vs 53.3% in dementia). 3) Problematic sexual behaviors seen among dementia patients were obscene remarks/sexual hallucination (30.8%, respectively), Public ex-posure (15.4%), inappropriate contact/physical contact with others/sexual seduction toward other patients (1.7%, respec-tively) in that order. Appea-rance time of sexual problems was over 1 year of dementia (50.0%), within 1 year of dementia (32.4%), and with the initiation of dementia (17.6%). The frequency was more than once a week in 60.0% and less than once a week in 40.0% of dementia. Place of sexual misbehavior was one's own room (68.6%), other place (22.9%), toilet (5.7%), activity therapy room (2.9%) in that order. Targets of sexual misbehavior were other patients (40.0%), caregivers (31.4%), medical personnel/others (14.3%, respectively) in that order. For the management of sexual misbehavior, most of the doctor schose non-pharmacological methods (83.9%). Conclusion : The sexual activity among Korean elderly with age over 60 was more active in healty controls than dementic patients, while sexual dysfunction except sexual desire disorder was more in dementic patients than healthy controls. Therefore, special interest and proper management was needed toward hypersexuality as well as sexual hypoactivity, and most of all, Con-ceptual changes of doctors and caregivers toward sexual problems in the elderly must be preceded.