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쥐 뇌의 Q-dianisidine Peroxidase의 특성 및 부분 정제
정태숙,이명은,최명언,Jeong, Tae-Sook,Lee, Myung-Eun,Choi, Myung-Un 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.1
뇌에서의 catecholamine 대사와 $H_2O_2$ 이용에 관한 기초 연구로 뇌의 peroxidase를 용해시켜 분광학적 방법으로 그 특성을 연구하였다. 쥐 뇌의 peroxidase를 0.05% Triton X-100, 0.2 M KCl를 포함하는 pH 7.4의 완충용액으로 용해시켰으며, 이 용해된 효소는 pH 5.2-5.3 사이에서 최고 활성도를 보였고 기질인 O-danisidine과 $H_2O_2$에 대한 $K_m$ 값은 각각 0.19 mM과 7.0 mM로 얻어졌다. 이들 기초 결과로부터 용해된 효소의 표준분석조건을 수립하였다. 이 효소의 정제방법으로 sephadex G-200 젤 여과법과 DEAE-cellulose 이온교환 크로마토그래피법을 사용하였으며 30% 회수율로 27배 가량의 부분 정제가 이루어졌다. 또한 이 효소의 기질에 관한 특수성도 살펴 보았다. For a basic study of catecholamine metabolism and of the utilization of hydrogen peroxide in brain. rat brain peroxidase was solubilized and its properties were examined spectrophotometrically. The peroxidase was solubilized in 0.01 M phosphate buffer, pH 7.4 containing 0.05% Triton X-100 and 0.2 M KCl. The $K_m$ values of brain peroxidase for O-dianisidine and $H_2O_2$ were 0.19 mM and 7.0 mM respectively. From these basic results, the standard assay condition of solubilized enzyme was established. The peroxidase was partially purified from solubilized protein by sephadex G-200 gel filtration and DEAE-cellulose ion exchange chromatography. The specific activity of brain peroxidase was increased 27-fold with 30% recovery. Substrate specificity of the enzyme was also investigated.
쥐 뇌의 O - dianisidine Peroxidase 의 특성 및 부분 정제
정태숙,이명은,최명언 ( Tae Sook Jeong,Myung Eun Lee,Myung Un Choi ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.1
For a basic study of catecholamine metabolism and of the utilization of hydrogen peroxide in brain, rat brain peroxidase was solubilized and its properties were examined spectrophotometrically. The peroxidase was solubilized in 0.01 M phosphate buffer, pH 7.4 containing 0.05% Triton X-100 and 0.2 M KCI. The K_m values of brain peroxidase for 0-dianisidine and H₂O were 0.19 mM and 7.0 mM respectively. From these basic results, the standard assay condition of solubilized enzyme was established. The peroxidase was partially purified from solubilized protein by sephadex G-200 gel filtration and DEAE-cellulose ion exchange chromatography. The specific activity of brain peroxidase was increased 27-fold with 30% recovery. Substrate specificity of the enzyme was also investigated.
어머니의 근무시간과 가사노동시간 및 가정-직장간 갈등이 자녀양육방식과 유아의 문제행동에 미치는 영향
정태숙(Jeong, Tae-Suk),김영희(Kim, Yeong-Hee) 한국유아교육·보육복지학회 2014 유아교육·보육복지연구 Vol.18 No.2
본 연구는 어머니의 근무시간과 가사노동시간 및 가정-직장간 갈등이 자녀양육방식과 유아의 문제행동에 어떠한 영향을 미치는지를 살펴보는 데 그 목적이 있다. 연구대상은 C도 소재의 유아교육기관에 소속된 만3~6세 유아기 자녀를 둔 취업모 330명으로 하였고, 자료 수집은 질문지 방식으로 유의표집하였다. 본 연구에서 수집된 자료는 SPSS 12.0을 이용하여 분석하였고, 이론적 경로모형은 AMOS 7.0 프로그램을 이용하여 검증되었다. 본 연구의 결과는 첫째, 유아의 외현화 문제행동은 어머니의 애정적 양육방식이 낮을수록, 거부적 양육방식이 높을수록, 어머니의 가정생활이 직장으로 유입되는 가정-직장간 갈등이 높을수록 높게 나타났다. 유아의 내면화 문제행동은 어머니의 거부적 양육방식이 높을수록, 어머니의 가정생활이 직장으로 유입되는 가정-직장 갈등이 높을수록 높게 나타난 반면 어머니의 애정적 양육방식은 직접적인 영향을 미치지 않았다. 둘째, 어머니의 애정적 양육방식은 어머니의 근무시간이 길수록, 어머니의 직장생활에서 가정으로 유입되는 직장-가정 갈등이 낮을수록 높게 나타난 반면 어머니의 가사노동시간이 길수록, 어머니의 가정 생활에서 직장으로 유입되는 가정-직장 갈등이 높을수록, 어머니의 직장생활에서 가정으로 유입되는 직장-가정 갈등이 높을수록 어머니의 거부적 양육방식이 높게 나타났다. 셋째, 어머니의 직장근무시간이 많을수록 어머니의 가정-직장 갈등과 직장-가정 갈등이 모두 높아진 반면 어머니의 가사노동시간은 직장-가정 갈등에만 영향을 주었다. 넷째, 어머니의 가정-직장 갈등은 어머니의 직장근무시간을 매개하여 어머니의 거부적 양육방식과 유아의 외현화, 내면화 문제행동에 간접적인 영향을 미쳤다. 반면 어머니의 직장-가정 갈등은 어머니의 직장근무시간과 가사노동시간을 매개하여 어머니의 애정적 양육방식, 거부적 양육방식, 유아의 외현화, 내면화 문제행동에 간접적인 영향을 미쳤다. The purpose of this study was to examine the effects of mother’s working and household work hours and family-work conflicts on parenting style and young children’s problem behavior. Subjects of this study were 330 working mothers who had 3 to 6 year-old preschoolers attending 6 kindergartens located in Chungbuk province. Data were analyzed by SPSS 12.0 program. The testing of model was done with analysis of correlational matrix in AMOS 7.0 package. The results of this study were as follows: First, family spillover work conflict, mother’s affectional and rejecting parenting style had a direct effect on children’s externalizing problem behavior. Children’s internalizing problem behavior was influenced by family spillover work conflict and mother’s rejecting parenting style. Second, mother’s working hours and work spillover family conflict had a direct on mother’s affectional parenting style while mother’s household work hours, family spillover work conflict, and work spillover family conflict had a direct on mother’s rejecting parenting style. Third, mother’s working hours had a direct on either family spillover work conflict or work spillover family conflict while mother’s household working hours had a direct effect only on work spillover family conflict. Fourth, family spillover work conflict had a mediating effect on mother’s working hours and indirectly influenced on mother’s rejecting parenting style, children’s externalizing and internalizing problem behaviors. Mother’s work spillover family conflict had a mediating effect on mother’s working and household working hours and indirectly influenced on mother’s rejecting and affectional parenting style, and children’s externalizing and internalizing problem behaviors. Also, mother’s affectional parenting style had a mediating effect on children’s externalizing problem behaviors while it did not have an indirect effect on children’s internalizing problem behaviors.
정태숙(Jeong, Tae-Sook),박선희(Park, Sun-Hee),김영희(Kim, Yeong-Hee) 충북대학교 생활과학연구소 2017 생활과학연구논총 Vol.21 No.2
This study examined the relative contributions of socio-demographic factors, physical health, mental health, family communication, and family conflict to family happiness across age groups. The participants of the study were 560 adults having at least one child under the age of 18. Data were analyzed through hierarchical regression using SPSS 18.0. For participants in their thirties, physical health and family communication were positively associated with family happiness, whereas family conflict was negatively associated with family happiness. For participants in their forties, family happiness was associated with family communication, mental health, and family conflict, in that order. Family monthly income in particular showed a substantial positive association with family happiness for participants in their fifties. Further, family communication, family conflict, and mental health were, in that order, related to family happiness for participants in their fifties. Family communication was the most strongly associated with family happiness regardless of the age group. These findings extend previous research on happiness in cultural contexts.