한 종합병원에서의 1994년도 신생아와 영아의 혈액배양 결과

정옥봉 기전여자대학 1995 論文集 Vol.15 No.-

This study was analyzed the results of isolated bacteriae from blood culture of neonate and infant at a general hospital in Chon-Ju City, 1994. The following results were obtained ; 1. Out of total 849 blood specimens cultured, 95 obtained from 86 Patients were found positive, thus representing the rate of positivity as 11.0% Positive culture ratio in the aged, neonate group showed 11.1% and infant group showed 10.6% 2. Distribution of bacteriae involved in patients with bacteriologically proven bacteremia were facultative anaerobic Gram neg. rods 46.2%, Gram positive cocci 37.4%, glucose nonfermentative Gram neg. rods 16%. The most commonly isolated pathogens were Enterobacter cloacae(24.2%), Kl. pneumoniae(8.8%), E. coli(8.8%), S. aureus(8.8%). The ratio of relative isolation of Alcaligenes xylasoxidans spp. among glucose nonfermentative Gram neg. rods were increased. 3. Different bacteriae isolation in additional blood culture (bacteremic superinfection) were found in 7.1% of patients with positive blood culture and they were all neonatal patients and frequently isolated bacteriae in decreasing order were Kl. pneumoniae, Enterobacter cloacae, and S. aureus.

전주시내 한 종합병원에서의 1992년도 혈액배양 결과

정옥봉 기전여자대학 1993 論文集 Vol.13 No.-

Total 6315 blood specimens from an undetermined number patients were cultured at a general hospital in Chon-Ju City, 1992. Laboratory data were analyzed and following results were obtained. 1) A total of 6315 blood specimens were cultured and 507 positive results (437 patients) were obtained 2) Distribution of organisms involved in patients with bacteriologically proven bacteremia were gram-negative bacilli 48.3%, glucose non-fermenters 30.7%, gram-positive cocci 17.2%, anaerobes 1.8%, and fungi 2.1%. The most commonly isolated pathogens were S. aureus, K. pneumoniae, Enterobacter, E.cozi, P.cepacia, P. aeruginosa and Aeromonas hydrophilia were an important new pathogen. 3) Polymicrobial bacteremia were found in 8.9% of patients with positive blood cultures. Most of the organism involved were E.coli, S.aureus, K.pneumoniae. 4) Salmonella typhi was susceptable to commonly used antimicrobial agents but many of the isolates of S. aureus, E. coli, and P. aeruginosa were resistant to routinely tested antimicrobial agents.

局所組織 Calcergy에 關한 光學 및 電子顯微鏡的 硏究

李武三,鄭玉峯,金永順 전북대학교 의과학연구소 1984 全北醫大論文集 Vol.8 No.1

The purpose of this study was to investigate the local mastocalcergy induced by lead acetate(2mg/kg) i.v. and compound 48/80(0.1%) 0.25ml s.c. Histochemical and electron microscopic studies were carried out on the skin taken from the rats which were sacrificed at various time interval after the injection of drugs.

흰쥐에서 WGA-HRP와 pseudorabies virus를 이용한 정관의 신경로에 대한 연구

이창현,정옥봉,고병문,이봉희,김수명,김인식,양홍현,Lee, Chang-Hyun,Chung, Ok-Bong,Ko, Byung-Moon,Lee, Bong-Hee,Kim, Soo-Myung,Kim, In-Shik,Yang, Hong-Hyun 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.1

This experimental studies was to investigate the location of PNS and CNS labeled neurons following injection of 2% WGA-HRP and pseudorabies virus (PRY), Bartha strain, into the ductus deferens of rats. After survival times 4-5 days following injection of 2% WGA-HRP and PRV, the rats were perfused, and their brain, spinal cord, sympathetic ganglia and spinal ganglia were frozen sectioned ($30{\mu}m$). These sections were stained by HRP histochemical and PRY inummohistochemical staining methods, and observed with light microscope. The results were as follows ; 1. The location of sympathetic ganglia projecting to the ductus deferens were observed in pelvic ganglion, inferior mesenteric ganglion and L1-6 lwnbar sympathetic ganglia. 2. The location of spinal ganglia projecting to the ductus deferens were observed in T13-L6 spinal ganglia. 3. The PRY labeled neurons projecting to the ductus deferens were observed in lateral spinal nucleus, lamina I, II and X of cervical segments. In thoracic segments, PRY labeled neurons were observed in dorsomedial part of lamina I, II and III, and dorsolateral part of lamina IV and V. Densely labeled neurons were observed in intermediolateral nucleus. In first lumbar segment, labeled neurons were observed in intermediolateral nucleus and dorsal commisural nucleus. In sixth lumbar segment and sacral segments, dense labeled neurons were observed in sacral parasympathetic nuc., lamina IX and X. 4. In the medulla oblongata, PRV labeled neurons projecting to the ductus deferens were observed in the trigeminal spinal nuc., A1 noradrenalin cells/C1 adrenalin cells/caudoventrolateral reticular nuc., rostroventrolateral reticular nuc., area postrema, nuc. tractus solitarius, raphe obscurus nuc., raphe pallidus nuc., raphe magnus nuc., parapyramidal nuc., lateral reticular nuc., gigantocellular reticular nuc.. 5. In the pons, PRV labeled neurons projecting to the ductus deferens were ohserved in parabrachial nuc., Kolliker-Fuse nuc., locus cooruleus, subcooruleus nuc. and AS noradrenalin cells. 6. In midbrain, PRV labeled neurons projecting to the ductus deferens were observed in periaqueductal gray substance, substantia nigra and dorsal raphe nuc.. 7. In the diencephalon, PRV labeled neurons projecting to the ductus deferens were observed in paraventricular hypahalamic nuc., lateral hypothalamic nuc., retrochiasmatic nuc. and ventromedial hypothalamic nuc.. 8. In cerebrum, PRV labeled neurons projecting to the ductus deferens were observed in area 1 of parietal cortex. These results suggest that WGA-HRP labeled neurons of the spinal cord projecting to the rat ductus deferens might be the first-order neurons related to the viscero-somatic sensory and sympathetic postganglionic neurons, and PRV labeled neurons of the brain and spinal cord may be the second and third-order neurons response to the movement of smooth muscles in ductus deferens. These PRV labeled neurons may be central autonomic center related to the integration and modulation of reflex control linked to the sensory and motor system monitaing the internal environment. These observations provide evidence for previously unknown projections from ductus deferens to spinal cord and brain which may be play an important neuroanatornical basic evidence in the regulation of ductus deferens function.

白鼠 口蓋의 實驗的 創傷 治癒 時期에 따른 肥滿細胞의 數的 變化

千景斗,鄭玉峰,李武三 전북대학교 의과학연구소 1985 全北醫大論文集 Vol.9 No.2

The changes in palatal mast cell numbers as healing time(1day, 3day, 6day, 10day, 14day) after experimental wounds were studied. Mast cells in each field which is 200㎛ distant on the medial and lateral side from the lateral side of greater palatine nerve are counted. The following conclusions are obtained. Healing of wound progressed rapidly between 3day and 6day. The greatest change in mast cell numbers as a distance occurred within the fields 200㎛ distant on the medial side from greater palatine nerve. In the fields on the medial side from greater palatine nerve, mean of mast cell numbers decreased significantly less than that of control rat in 3 day group and increased significantly more than that of 3 day group in 14 day group. Whereas the paucity of mast cells within the 200㎛ distant of medial side from greater palatine nerve was evident in 3day group, near normal levels were achieved by 6 day group. Mast cells which observed in 10 and 14 day groups were almost small size.

初生雛 胃粘膜內 粘液物質의 組織化學的 硏究

李武三,白泳基,鄭玉峰 전북대학교 의과학연구소 1985 全北醫大論文集 Vol.9 No.1

This studies were designed to investigate histochemical propeites and sequntial changes of the mucosubtances according to aging in the young chicken. The tissues were taken from the ventriculus and proventriculus at the 1day, 1week, 2weeks, and 3weeks after hatching. The mucosubstances of the tunica mucosa stained with alcian blue pH 2.5, alcian blue pH 1.0, periodic acid Schiff(PAS), alcian blue pH 2.5-PAS, aldehyde fuchsin pH 1.7-alcian blue pH 2.5 staining. The results obtained were as follows : 1. In the mucosa of the proventriculus and ventriculus at all stages, the mucosubstances were composed both of acid and neutral mucin. The surface epithelial cells of upper 1/3 of the proventriculus at the 1day, 1week and 2weeks more abundant in neutral mucin than acid mucin, but they contained more acid mucin than neutral mucin at the 3weeks. The surface epithelial cells of lower 2/3 at the 1day and 1week has more acid mucin than neutral mucin, but at the 2weeks and 3weeks content of neutral mucin became increased. 2. In the surface epithelial cells of ventriculus, the mucosubstances were composed of mainly neutral mucin at the 1day after hatching, but content of acid mucin were much more than that of neutral mucin at the 1, 2, and 3weeks. The cells of gastric pits contained with mainly neutral mucin in early stage, but gradually exchanged to acid mucin in the process of time. 3. The mucosubstances in the surface epithelial cells of proventriculus were composed of sulfated and nonsulfated mucin. The surface epithelial cells of upeer 1/3 of proventriculus were mainly nonsulfated mucin before 2weeks, but they were prominent sulfated mucin in 3weeks after hatching.

병원성(病原性) 포도구균(葡萄球菌)의 동정(同定)을 위한 Coagulase, Deoxyribonuclease (DNase) 및 내열성(耐熱性) Nuclease 생산능(生産能)의 비교(比較)

김종면,송희종,정옥봉,Kim, Jong-Myeon,Song, Hee-Jong,Jeong, Ok-Vong 대한수의학회 1981 大韓獸醫學會誌 Vol.21 No.2

A total of 251 clinical isolates (human origin, 43 strains and bovine udder origin 208 strains) of the Staphylococcus that fermented mannitol aerobically were tested for their ability to produce coagulase, DNase, and thermostable nuclease. Of these, 158 isolates coagulated human or bovine plasma, produced DNase, and thermostable, nuclease and were identified as St. aureous, 146 of which produced a 1+ to 3+ clot. The remaining 12 isolated produced a -clot in citrate treated plasma but produced 1+ to 3+ clot in ethylenedi-aminetetraacetic acid (EDTA) treated plasma. It was found that 7 coagulase positive isolates failed to produced thermostable nuclease. In these organisms, we found out of the clot formation is not by coagulase activity but utilization of citrate, because EDTA treated plasma is not coagulated. Among 93 isolates which did not coagulate citrate-or EDTA treated plasma and thermostable nuclease negative, 28 strains produced DNase were identified as St. epidermidis, and other strains were not identification further. It was found that thermostable nuclese production appears to be a consistent property of St. aureus and the test is easy to perform, is rapid became quite distinct within 2 to 4 hour, and is not influenced by as many factors and variations as the coagulase test.

흰쥐에서 WGA-HRP와 Pseudorabies virus를 이용한 부고환의 신경로에 대한 연구

이창현,고병문,이봉희,정옥봉 대한해부학회 2001 Anatomy & Cell Biology Vol.34 No.2

Glucose Oxidase에 의해 손상된 배양된 신경아세포에 미치는 국화 추출물의 효과

제갈승주,서영미,박승택,임요섭,정옥봉 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.2

To clarify the oxidative stress of reactive oxygen species (ROS) and the effect of Chrysanthemum morifolium L. (CM) flower extract on the cultured neuroglial cells (C6 glioma) damaged by ROS, cell adhesion effect was measured by colorimetric assay after cultured C6 glioma cells were treated with various concentrations of glucose oxidase (GO) for 5 hours. For the antioxidative effect of CM flower extract, cell adhesion activity (CAA), superoxide dismutase (SOD)-like activity and lactate dehydrogenase (LDH) activity were assessed against GO-induced cytotoxicity on same cultures. In this study, GO remarkably decreased CAA dose-dependently, and the XTT90 and XTT50 values were measured at 15 mU/mL and 50 mU/mL following the treatment of C6 glioma cells with 5∼60 mU/mL of GO. The CM flower extract significantly increased cell adhesion activity damaged by GO-induced cytotoxicity, and it also showed the SOD-like activity and the decrease of LDH activity. From these results, it is suggested that GO was cytotoxic on cultured C6 glioma cells, and CM flower extract showed antioxidative effects as shown by the increased CAA, SOD-like activity and the decrease of LDH activity on GO-induced cytotoxicity on the same cultures. .

흰쥐 숨뇌의 맨아래구역에서 Phenylalanine처리에 따른 Monosodium Glutamate의 효과

이창헌,고병문,김명순,정옥봉 대한췌담도연구회 2000 대한췌담도연구회지 Vol.5 No.2