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도시 녹화를 위한 질소고정 균 선별 및 식물 생장 평가
정순환,이상섭 한국미생물·생명공학회 2018 한국미생물·생명공학회지 Vol.46 No.2
Currently, urban greening projects and research are attracting attention as a way to mitigate urban heat island phenomenon. In this study, nitrogen fixative bacteria were isolated and their effects on plant growth were confirmed. First, enrichment was performed in a nitrogen-free medium to isolate the nitrogen- fixing bacteria, and the colony showing high growth in a medium with limited nitrogen source was isolated and purified. Separated bacterial isolates were reduced by more than 90% acetylene by ARA and indirectly confirmed the activity of nitrogenase by ethylene production. Cedecea sp. MK7 and Enterobacter sp. Y8 with confirmed reproducibility were selected as nitrogen fixative bacteria. Nitrogen fixing bacteria were applied to the growth of perennial rye grass, and it was found that the dry weight increased to 34.80 mg (186.60%) compared with the control with 18.65 mg dry weight. After plant growth, microbial community analysis of soil applied by bacteria showed similarity to the control group. Therefore, in this study, it is expected that the efficiency will be increased if plant growth is promoted by using nitrogen fixing bacteria in urban greenery system. 현재 도시의 도시열섬현상을 완화시키는 방안으로 도시 녹화사업 및 연구가 주목 받고 있다. 이 연구에서는 질소고정균을 분리하고, 식물 성장에 미치는 영향을 확인했다. 먼저질소고정 균을 분리하기 위해, 질소원이 없는 배지에서enrichment를 실시했고, 질소원이 제한된 배지에서 높은 성장을 보인 colony를 분리하여 순수분리 했다. 순수 분리된 균은 ARA를 통해 acetylene이 90% 이상 감소되고, ethylene 생성을 통해 nitrogenase의 활성을 간접적으로 확인했다. 재현성이 확인된 Cedecea sp. MK7과 Enterobacter sp. Y8을선별했다. 선별된 질소고정 균을 perennial rye grass의 성장에 적용한 결과 건조중량이 18.65 mg인 대조군에 비해34.80 mg (186.60%)으로 증가한 것을 확인했다. 식물 성장후, 질소고정 균이 접종된 토양의 미생물 군집 분석은 대조군과 유사했다. 따라서 본 연구에서는 도시녹화 시스템에 질소고정 균을 이용하여 식물 성장을 촉진한다면 그 효율이 증대될 것이다.
丁順煥,吳貞姬 고려대학교 의과대학 1982 고려대 의대 잡지 Vol.19 No.1
It is important that there be easily replicated measures for demonstration the efficiency and effectiveness of services provided, as well as for transmitting information on patient functional status when transferring services responsibilities from one agency or facility to another. The authors used the PULSES profile and the Barthel index to measure severity of disability and to monitor rehabilitation progress over a span of time, for 87 brain dysfunction patients ranging from 18-25 years old(mean age:56.3yrs). The PULSES and Barthel scores were measured by medical records on Admission, Discharge, and by observation and interview on follow up. The results were as follows: 1. Rehabilitation programs were valuable to improve the functional level of patients. 2. The authors could easily evaluate patients functional status using the PULSES Profile and the Barthel Index. 3. The prognosis of patients was good below 16 in PULSES score, especially better below 12. 4. The prognosis of patients was good above 40 in Barthel score, especially better above 60.
丁順煥,吳貞姬 고려대학교 의과대학 1985 고려대 의대 잡지 Vol.22 No.1
It is generally accepted that the nerve conduction study is a valuable method in evaluation of peripheral neuropathy including entrapment syndrome. Meralgia paresthetica is a clinical benign entrapment neuropathy of the lateral removal cutaneous nerve of the thigh. It often occurs in association with obesity, pregnancy or diabetes, and may appear as a result of trauma to the pelvic bones or scanning in the lateral inguinal region. The diagnosis is usually based on clinical symptoms. This study is to establish the normal values of lateral femoral cutaneous nerve conduction velocity including latency, amplitude and duration of the evoked potential for the objective diagnostic aid. In this study. 120 nerves were measured bilaterally in 60 healthy subjects, 39 males and 21 females, ranging in age from 16 to ?? years of age. Nerve was stimulated at 1㎝ medial to the anterion superior. iliac spine by needle electrode and the action potential was recorded with surface electrodes 12 cm below the stimulating point: with antidromic method. The results are summarized as follows; 1. The mean conduction velocity of lateral femoral cutaneous nerve 47.96±4.81. M/sec. 2. The mean value of the latency to the peak of negative phase is 2.53±0.25msec. 3. The amplitude of the action potential is 11.27±4.23uV. 4. The mean value of the duration of the action potential is 1.09±0.39 msec. 5. Nostatistical variation is noted with advancing age in conduction velocity, latency, amplitude and duration of the evoked potential. 6. There are no significant differences related to sex and the side of the extremities examined.