PCR을 이용한 식품 내 Salmonella 균주의 신속 검출방법

정상훈,김묘영,김현중,김태운,유상렬,김해영,Jung, Sang-Hun,Kim, Myo-Young,Kim, Hyun-Joong,Kim, Tae-Woon,Ryu, Sang-Ryeol,Kim, Hae-Yeong 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.3

여러 종류의 식품에서 Salmonella 균주를 손쉽고 빠르게 검출하기 위하여, Salmonella 장독소 유전자(stn)를 기초로 제작한 primer (STN1, STN2)를 이용하여 PCR을 수행한 결과 617 hp의 특이적인 DNA단편을 얻을 수 있었다. PCR 민감도는 순수 배양한 균체에서 추출한 template DNA는 1 pg까지 검출이 가능하였고, 직접 균체를 template로 이용한 경우에는 $10^2\;cells$까지 검출이 가능하였다. Salmonella typhimurium을 인위적으로 접종시킨 식품에서는 식품 1 g당 $10^3{\sim}10^4$ cells까지 검출할 수 있었다. 이러한 결과는 PCR을 이용하여 Salmonella에 오염된 식품에서 이들 균주를 간편하고 신속하게 검출할 수 있을 것이다. This study was carried out to investigate the simple and rapid detection of Salmonella species in different kinds of food using PCR method. The specific primer sets (SIN1 and SIN2) was designed and utilized to amplify a 617 bp DNA fragment from salmonella species. The sensitivity of PCR was 1 pg of purified template DNA or $10^2$ cells from pure culture. The detection limit of Salmonella typhimurium on agarose gel electrophoresis was $10^3{\sim}10^4$ cells/g in the artificially contaminated food samples. These results suggested that this simple method could be applied to industrial fields for detection of Salmonella species in food.

PCR을 이용한 식품 내 Salmonella 균주의 신속 검출방법

정상훈 ( Jeong Sang Hun ),김묘영 ( Kim Myo Yeong ),김현중 ( Kim Hyeon Jung ),김태운 ( Kim Tae Un ),유상렬 ( Yu Sang Lyeol ),김해영 ( Kim Hae Yeong ) 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.3

This study was carried out to investigate the simple and rapid detection of Salmonella species in different kinds of food using PCR method. The specific primer sets (STN1 and STN2) was designed and utilized to amplify a 617 bp DNA fragment from Salmonella species. The sensitivity of PCR was 1 pg of purified template DNA or 10² cells from pure culture. The detection limit of Salmonella typhimurium on agarose gel electrophoresis was 10³~10⁴cells/g in the artificially contaminated food samples. These results suggested that this simple method could be applied to industrial fields for detection of Salmonella species in food.

Omitting radiation therapy in preoperative treatment of rectal cancer

정상훈(Sang Hun Jung) 대한외과학회 2014 대한외과학회 학술대회 초록집 Vol.2014 No.11

다양한 형상의 관내에서 화염전파시 틀립화염으로 전환되는 시간과 거리

정상훈(Sang-Hun Jung),이은도(Uen-Do Lee),김남일(Nam-IL Kim),신현동(Hyun-Dong Shin) 한국연소학회 2002 KOSCOSYMPOSIUM논문집 Vol.- No.-

황색육아종성염증을 동반한 맹장암

정상훈(Sang Hun Jung),김재황(Jae Hwang Kim) 대한외과학회 2008 Annals of Surgical Treatment and Research(ASRT) Vol.74 No.5

Xanthogranulomatous inflammation is a chronic inflammatory condition characterized by aggregation of lipid-laden foamy macrophages (xanthoma cells). This disease entity is wellrecognized in the kidney and gallbladder. However, involvement of the colon is extremely rare. Radiologically, xanthogranulomatous inflammation could be misinterpreted as a locally invasive cancerous lesion. Indeed, coexisting malignancy has been reported in xanthogranulomatous inflammation. In this case report, a woman complained of fever and right lower quadrant abdominal pain for a period of 5 days. A huge mass lesion with severe pericolic infiltration was found on abdominal CT scan. Right hemicolectomy was performed along with removal of the adjacent soft tissue and right ovary and fallopian tube. Pathology examination demonstrated xanthogranulomatous inflammation coexisting with cecal cancer (T3N0M0).

전이성대장암환자에서 체외 항암약물 감수성 검사와 일차항암화학요법 간의 임상적 연관성

정상훈(Sang Hun Jung),김소현(So Hyun Kim),김재황(Jae Hwang Kim) 대한종양외과학회 2015 Korean Journal of Clinical Oncology Vol.11 No.2

Purpose: The study investigated correlations between adenosine triphosphate-based chemotherapy response assay (ATP-CRA) and clinical response after first line chemotherapy for metastatic colorectal cancer patients. Methods: From June 2009 to June 2012, 24 patients were enrolled in this study. ATP-CRA was performed to evaluate the chemosensitivities of three combination anticancer drugs such as 5-fluorouracil (FU)+oxaliplatin and irinotecan, and capecitabine+oxaliplatin for metastatic colorectal cancer. A sensitive group (SG) and resistant group (RG) for chemotherapy regimen (oxaliplatin+5-FU+leucovorin [FOLFOX], n=14; oxaliplatin+capecitabine [CapeOx], n=2; irinotecan+5-FU+leucovorin [FOLFIRI], n=8) was defined as cut-off value 30%. The outcomes were a correlation between the ATP-CRA results and the clinical response and progression-free survival. Results: Patients were dichotomized into the SG (19 patients) and RG (5 patients) groups. There was no difference between groups, in terms of regimen, frequency and interval of chemotherapy. The disease control rate after first response evaluation was not shown statistically difference (89.5% in SG and 60.0% in RG, respectively, P=0.15). And there was also no significance in second and third response evaluation. The median progression-free month were 7.0 (range, 2–27 month) and 5.0 (range, 3–12 month), respectively and shown marginal significance. However, there was no statistical difference in progression-free survival curve (P=0.16). Sensitivity, specificity, positive prediction rate and negative prediction rage for disease control after first response was 85.0%, 50.0%, 89.5%, and 40.0%. Conclusion: Although ATP-CRA may be helpful in predicting chemotherapy response in metastatic colorectal cancer, there was not conclusive. Therefore, we recommend further clinical studies to confirm the efficacy of chemoresponse assay for metastatic colorectal cancer.

횡행결장의 황색육아종성염증

정상훈 ( Sang Hun Jung ) 영남대학교 기초/임상의학연구소 2007 Yeungnam University Journal of Medicine Vol.24 No.2S

Xanthogranulomatous inflammation is an uncommon benign inflammatory disease characterized by aggregation of lipid-laden foamy macrophages (xanthoma cells) that usually presents with tumor-like appearance. The clinical and radiological findings are suggestive of advanced carcinoma of involved organ. A 66-year-man presented fever, upper abdominal mass during 10 days. By computed tomography, barium enema and PET-CT, his case suspected transverse colon malignancy. A laparotomy was performed. On operation, huge mass originated from mid-transverse was identified and transverse colectomy was performed. The pathologic report demonstrated xanthogranulomatous inflammation in transverse colon.

실내공기질측정을 위한 NDIR 방식 복합가스측정시스템 설계 및 구현

정상훈(Sang Hun Jung),백승현(Seung Hyun Paik),정상우(Sang Woo Jung),박홍배(Hong Bae Park) 대한전자공학회 2016 대한전자공학회 학술대회 Vol.2016 No.6

In this paper, we design and implement NDIR multi-gas detecting system. The optical chamber and hardware board for indoor air quality(IAQ) measurement is studied. The optical chamber has three optical cavity and air circulation hole with optical shielding. One light and three Infrared detectors for CO2, CO, and NO2 are located in the optical chamber. The hardware board is designed to operate by low power, and has three amplifier circuit for each gas concentration range. We evaluate the performance of NDIR multi-gas detecting system by the experiment to measure the air quality standard gas concentration.

대장 신경내분비종양의 임상 경과와 병리 특징

정상훈 ( Sang Hun Jung ),김희철 ( Hee Cheol Kim ),유창식 ( Chang Sik Yu ),장흥문 ( Heung Moon Chang ),류민희 ( Min Hee Ryu ),이재련 ( Jae Lyun Lee ),김정선 ( Jung Sun Kim ),김진천 ( Jin Cheon Kim ) 대한소화기학회 2006 대한소화기학회지 Vol.48 No.2

목적: 대장의 신경내분비종양은 매우 드물고, 통상적인 대장암에 비해 빠르게 진행하며 예후가 매우 나쁘다. 그러나 발생 빈도가 적어 국내에는 아직까지 보고가 없다. 이번 연구는 후향 분석을 통해 대장의 신경내분비종양의 임상 경과 및 병리 특징을 알고자 하였다. 대상 및 방법: 1997년 6월부터 2004년 12월까지 서울아산병원에서 H&E 및 면역조직화학염색에 의해 조직학적으로 대장의 신경내분비종양으로 진단 받은 13예의 환자를 대상으로 하였으며, 대장의 신경내분비암종 10예와 대장의 샘암종에 신경내분비분화를 포함하는 혼합암종이 3예였다. 카르시노이드 종양은 대상에서 제외되었으며, 환자들의 기록은 후향으로 조사하였다. 결과: 4,512명 중 13예(0.3%)의 환자가 대장의 신경내분비종양으로 진단되었다(신경내분비암종 10예, 혼합암종 3예). 환자 나이의 중앙값은 60세(41-83세)였으며, 남자가 6예, 여자는 7예였다. 종양의 발생부위는 직장 9예, 에스자결장 2예, 그리고 맹장과 횡행결장이 각각 1예였다. 면역조직화학염색을 시행한 신경내분비암종 9예 모두 synaptophysin 양성인 반면, chromogranin A는 3예에서 양성이었다. 6차 AJCC TNM병기에 따라 IIIB 병기 2예, IIIC 병기 3예 그리고 IV 병기가 8예였으며, 대장의 신경내분비암종 환자에서는 10예 중 7예에서 원격전이가 있었다. 신경내분비암종 10예와 혼합암종 3예의 중간생존율은 각각 16.4개월과 30개월이었다. 10예의 신경내분비암종 환자 중 항암치료를 받은 5예의 중간생존율은 III 병기 3예와 IV 병기 2예에서 각각 32개월과 17.5개월이었으나, 항암치료를 받지 않은 5예의 중간생존율은 6.2개월이었다. 혼합암종 3예는 모두 항암치료를 받았으며 중간생존율은 30개월이었다. 결론: 대장의 신경내분비종양의 빈도는 0.3%로 매우 드물다. 지금까지 적은 수의 환자와 빠르게 진행되는 종양의 특성으로 유용한 치료방침이 없었으나, 적극적인 병소의 절제와 etoposide/cisplatin을 이용한 항암치료 및 방사선치료는 환자의 생존율을 향상시킬 수 있을 것으로 기대한다. Background/Aims: Colorectal neuroendocrine carcinoma is a rare neoplasm exhibiting fulminant progression and having poor prognosis. The purpose of this study is to verify the clinicopathologic characteristics of colorectal neuroendocrine carcinoma. Methods: From June 1997 to December 2004 at Asan Medical Center, ten patients were originally identified as colorectal neuroendocrine carcinoma on the basis of H&E and immunohistochemical staining (IHC). Carcinoid tumors were excluded in this study. Medical records of thirteen patients were reviewed retrospectively. Results: Ten patients (0.2%) with colorectal neuroendocrine tumors were identified from 4,512patients with colorectal cancer; ten neuroendocrine carcinomas and three adenocarcinomas with neuroendocrine differentiation. Their median age was 60 (41-83) years. The subjects consisted of six males and seven females. Nine tumors were located in the rectum, two in the sigmoid, and each one in the transverse colon and cecum, respectively. Nine of ten neuroendocrine carcinomas expressed synaptophysin, but chromogranin A were expressed in four. All patients were advanced at the time of diagnosis, with AJCC TNM staging: stage IIIB (n= 2), stage IIIC (n=3), and stage IV (n=8). The median survival for ten neuroendocrine carcinomas and three adenocar-cinomas with neuroendocrine differentiation were 16.4 months and 30 months, respectively. Five patients who received chemotherapy showed median survival of 32 months (stage III) and 17.5 months (stage IV), whereas other five patients without chemotherapy died with a median survival of 6.2 months. Conclusions: Colorectal neuroendocrine tumors are extremely rare showing aggressive behavior biologically, i.e fulminant early distant metastasis. Nevertheless, improved survival may be achieved by aggressive multimodality therapy. (Korean J Gastroenterol 2006;48:97-103)

직장암의 치료

정상훈 ( Sang Hun Jung ),김재황 ( Jae Hwang Kim ),심민철 ( Min Chul Shim ) 영남대학교 기초/임상의학연구소 2007 Yeungnam University Journal of Medicine Vol.24 No.2S

Treatment for rectal cancer continues to develop towards the improved local control and overall survival, maintaining quality of life, and preserving sphincter, genitourinary, and sexual function. Preoperative assessment for tumor depth, lymph node, and distant metastasis has an important role on treatment plan. Preoperative staging is used to determine the indication for neoadjuvant therapy as well as the indication for local excision versus radical cancer resection. Local excision is likely to be curative in patients whose tumor are confined to the submucosa without regional lymph and systemic metastasis. Total mesorectal excision (TME) and autonomic nerve preservation are standard procedure for advanced rectal cancer. In patients with advanced tumor stage (T3/T4 and/or N1) with no distant metastasis, preoperative chemoradiation followed by radical resection has become widely accepted recently.