Antiviral effect of dietary germanium biotite supplementation in pigs experimentally infected with porcine reproductive and respiratory syndrome virus

정복기,이진아,이봉주 대한수의학회 2013 JOURNAL OF VETERINARY SCIENCE Vol.14 No.2

Germanium biotite (GB) is an aluminosilicate mineral containing 36 ppm germanium. The present study was conducted to better understand the effects of GB on immune responses in a mouse model, and to demonstrate the clearance effects of this mineral against Porcine reproductive and respiratory syndrome virus (PRRSV) in experimentally infected pigs as an initial step towards the development of a feed supplement that woul promote immune activity and help prevent diseases. In the mouse model, dietary supplementation with GB enhanced concanavalin A (ConA)-induced lymphocyte proliferation and increased the percentage of CD3+CD8+ T lymphocytes. In pigs experimentally infected with PRRSV, viral titers in lungs and lymphoid tissues from the GB-fed group were significantly decreased compared to those of the control group 12 days post-infection. Corresponding histopathological analyses demonstrated that GB-fed pigs displayed less severe pathological changes associated with PRRSV infection compared to the control group, indicating that GB promotes PRRSV clearance. These antiviral effects in pigs may be related to the ability of GB to increase CD3+CD8+ T lymphocyte production observed in the mice. Hence, this mineral may be an effective feed supplement for increasing immune activity and preventing disease.

Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice

정복기,Sun-Ju Cho,Jae-Hyung Ko,Bong-Joo Lee 대한수의학회 2010 JOURNAL OF VETERINARY SCIENCE Vol.11 No.3

Interleukin (IL)-10 exerts potent anti-inflammatory effects by suppression of both T-help (Th) 1 and Th2 cells. Previous studies have reported that IL-10 can ameliorate various inflammatory disorders. The present study was performed to examine whether IL-10 plasmid DNA could suppress development of atopic dermatitis (AD)-like skin lesions in NC/Nga mice, as an initial step towards the development of an appliance for use in dogs with AD. Intradermal injection of IL-10 plasmid DNA markedly inhibited the development of AD-like skin lesions, as evidenced by a marked decrease in skin symptoms and reduced inflammation within the skin lesions. Efficacy was confirmed by significant decreases in eosinophil ratio and serum IgE concentration, and a reduction in the number of Staphylococcus aureus recovered from the ear. Moreover, relative mRNA expression levels of IL-4 and interferon-γ in the skin lesions of mice injected with IL-10plasmid DNA were also decreased compared with those of control mice. Of note, higher serum IL-10 levels in mice injected with IL-10 plasmid DNA were maintained compared with those in control mice. Taken together, the results indicate that IL-10 plasmid DNA can suppress the development of AD-like skin lesions by suppressing both Th1 and Th2 cell responses. Beneficial effects of IL-10 plasmid DNA may be expected in dogs with AD.

Lateral Oropharyngeal Wall Coverage with Buccinator Myomucosal and Buccal Fat Pad Flaps

정복기,송승용,김소현,김영석,이원재,홍종원,노태석,유대현,김세헌 대한성형외과학회 2015 Archives of Plastic Surgery Vol.42 No.4

Background Reconstruction of oropharyngeal defects after resection of oropharyngeal cancer is a significant challenge. The purpose of this study is to introduce reconstruction using a combination of a buccinator myomucosal flap and a buccal fat pad flap after cancer excision and to discuss the associated anatomy, surgical procedure, and clinical applications. Methods In our study, a combination of a buccinator myomucosal flap with a buccal fat pad flap was utilized for reconstruction after resection of oropharyngeal cancer, performed between 2013 and 2015. After oropharyngectomy, the defect with exposed vital structures was noted. A buccinator myomucosal flap was designed and elevated after an assessment of the flap pedicle. Without requiring an additional procedure, a buccal fat pad flap was easily harvested in the same field and gently pulled to obtain sufficient volume. The flaps were rotated and covered the defect. In addition, using cadaver dissections, we investigated the feasibility of transposing the flaps into the lateral oropharyngeal defect. Results The reconstruction was performed in patients with squamous cell carcinoma. The largest tumor size was 5 cm×2 cm (length×width). All donor sites were closed primarily. The flaps were completely epithelialized after four weeks, and the patients were followed up for at least six months. There were no flap failures or postoperative wound complications. All patients were without dietary restrictions, and no patient had problems related to mouth opening, swallowing, or speech. Conclusions A buccinator myomucosal flap with a buccal fat pad flap is a reliable and valuable option in the reconstruction of oropharyngeal defects after cancer resection for maintaining functionality.

Immunoprophylactic Effects of Shiitake Mushroom (Lentinula edodes) against Bordetella bronchiseptica in Mice

정복기,이진아,이봉주 한국미생물학회 2012 The journal of microbiology Vol.50 No.6

Antimicrobials are used as feed additives to improve growth performance and to prevent subclinical disease challenge in industrial animals. However, these drugs can lead to the development of resistant strains of bacteria. Shiitake mushrooms (SM) (Lentinula edodes) have long been popular as a health food in East Asia. Moreover, SM-derived polysaccharides are well-known as immunostimulants that possess antimicrobial properties. The aim of the present study was to evaluate the immunoprophylactic effects of SM against Bordetella bronchiseptica infection in mice as an initial step towards the development of eco-friendly feed additives to reduce the use of antimicrobials. Although SM had no effect on body weight gain under the un-infected conditions, SM alleviated progressive weight loss and helped in the recovery of body weight in B. bronchiseptica infected mice. Dietary supplementation with SM reinforced bacterial clearance in the infected mice. Of note, SM markedly increased the percentage of various T lymphocytes and the relative mRNA expression levels of tumor necrosis factor-α and interferon-γ in the bronchial lymph node early in the infection. Taken together, these findings suggest that SM could help in the improvement of body weight gain during B. bronchiseptica infection and may enhance the protective immune activity against a subclinical disease challenge, such as B. bronchiseptica infection in mice, probably by a strong stimulation of non-specific immune responses. Hence, SM may provide an alternative to reduce use of antimicrobials. Confirmation of the beneficial effects of SM as a feed additive is now required in industrial animals.

An effective aesthetic toe-shortening procedure

정복기,김윤정,이영대,김영석 대한미용성형외과학회 2023 Archives of Aesthetic Plastic Surgery Vol.29 No.2

Background Many people with a longer second toe or lesser toes experience symptoms such as corns, hammertoe, and numerous others, especially when wearing open-toe shoes. Proximal interphalangeal joint arthrodesis using intraosseous loop wiring performed through a hidden side incision is a useful method to shorten the lesser toes aesthetically. Methods Aesthetic toe-shortening procedures were performed in 30 patients. All patients were evaluated by a physical examination and X-rays, and they underwent proximal interphalangeal joint arthrodesis using intraosseous loop wiring through a medial incision. Demographic characteristics, including foot morphology, were analyzed. The number of resected toes and resection amounts of each toe were measured. Patients’ satisfaction was determined through a questionnaire administered at each follow-up. Results In total, 91 toe-shortening procedures were performed in 30 patients who were followed up for an average of 24 months (range, 6–48 months). Sixteen patients had Greek-type feet (53.3%) and 14 had square-type feet (46.7%). Twelve patients had hammer toe deformity (40.0%) and 13 had corns (43.3%). The average length of the resected second and third toes was 9.66±2.79 mm (range, 5–15 mm) and 7.78±2.51 mm (range, 5–12 mm), respectively. The vast majority of patients were satisfied with the final results. No significant complications such as nonunion occurred. Only one case of mild angulation of the second toe was noted. Conclusions Aesthetic toe-shortening using the procedure described here can prevent the development of lessor toe deformities and provide permanent, aesthetically pleasing results with a short recovery time

Effect of Relaxin Expressing Adenovirus on Scar Remodeling: A Preliminary Study

정복기,이원재,강은혜,안효민,김용욱,나동균,윤채옥,윤인식 대한두개안면성형외과학회 2017 Archives of Craniofacial Surgery Vol.18 No.1

Background: Relaxin is a transforming growth factor β1 antagonist. To determine the effects of relaxin on scar reduction, we investigated the scar remodeling process by injecting relaxin-expressing adenoviruses using a pig scar model. Methods: Scars with full thickness were generated on the backs of Yorkshire pigs. Scars were divided into two groups (relaxin [RLX] and Control). Adenoviruses were injected into the RLX (expressing relaxin) and Control (not expressing relaxin) groups. Changes in the surface areas, color index and pliability of scars were compared. Results: Fifty days after treatment, the surface areas of scars decreased, the color of scars was normalized, and the pliability of scars increased in RLX group. Conclusion: Relaxin-expressing adenoviruses improved the surface area, color, and pliability of scars. The mechanism of therapeutic effects on scar formation should be further investigated.

새뜰마을사업을 시작하면서 (19년 선정지역) : 곡성군_'19년 선정

정복기 한국농촌건축학회 2019 한국농촌건축학회 학술발표대회 Vol.2019 No.05

Kartagener's Syndrome의 1예

홍순표 · 정복기 · 조건국 조선대학교 의학연구원 1983 The Medical Journal of Chosun University Vol.8 No.1

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Kartagener's Syndrome의 1예

홍순표,정복기,조건국 朝鮮大學校 附設 醫學硏究所 1983 The Medical Journal of Chosun University Vol.8 No.1

Kartagener's syndrome is a rare disease, characterized by situs inversus, sinusitis, dextrocardia and bronchiectasis. This is a report of a rare case of kartagener's syndrome in 20-year-old-male whose chief complaint was purulent sputum, rhinorrhea, & exertional dyspnea. Chest P-A view revealed the dextrocardia and the magenblase in the right subphrenic area, suggesting situs inversus totalis. Multiple small cystic radielu-concies are also seen in lower halves of both thorax, suggesting bronchiectasis. P.N.S view revealed the homogenous increase-d density in all sinuses, tut didn't clear bony destruction. Bronchogram revealed the saccular and tubular dilation of the segmental bronchi in all bronchi, Particularly in the right lower lobe bronchus. EKG finding was alternation of voltage, right atrial hypertrophy and dextrocardia.

Autologous Fat Graft in the Reconstructed Breast: Fat Absorption Rate and Safety based on Sonographic Identification

김홍열,정복기,유대현,이동원 대한성형외과학회 2014 Archives of Plastic Surgery Vol.41 No.6

Background: Autologous fat graft has become a useful technique for correction of acquiredcontour deformity in reconstructed breasts. However, there remains controversial regardingthe efficacy and safety of the practice for reconstructive breast surgery. Methods: A retrospective review was performed on 102 patients who had secondary fatgrafting after breast reconstruction. Fat harvest, refinement and injection were done byColeman’s technique. All patients were followed up postoperatively within 1 month and after6 months including physical examination and ultrasonography. In 38 patients, the reabsorptionrate was calculated by serial changes of thickness between skin and pectoral fascia in theultrasonic finding. Locoregional recurrence rate was compared with control group of 449patients who had breast reconstruction without fat graft in the same time period. Results: Average 49.3 mL fat was injected into each breast. The most common location of fatgraft was upper pole, followed by axilla, lower and medial breasts. During 28.7 months ofaverage follow-up period, 2.9% of total patients had symptoms of palpable mass on fat graftside and ultrasonography identified fat necrosis and cyst formation in 17.6% of the patients. Calculated fat reabsorption rate was 32.9%. Locoregional recurrence was occurred in 1 patient(0.9%) and the rate was not different significantly with control group (2%). Conclusions: Although further studies are required to provide surgeons with definitiveguidelines for the implementation of fat grafting, we propose autologous fat graft is anefficient and safe technique for secondary breast reconstruction.