우 동결수정란의 산업적 이용에 관한 연구

노환철(H . C . Rho),정광업(K . E . Chung),신규용(G . Y . Shin),정병현(B . H . Chung),백운화(U . H . Pek),정길생(K . S . Chung) 한국축산학회 1988 한국축산학회지 Vol.30 No.3

To establish the industrial utilization of frozen bovine embryo, bovine embryos were frozen to -196℃, storaged, thawed and transferred. The results obtained from these experiments are summarized as follows : 1. Superovulation was induced from 27 Holstein donor cows. The average numbers of corpus luteum per donor cow was 10.3. The egg recovery rate was 56.3%, and the average number of recovered embryo was 4.6 per donor cow, 2. The pregnancy rates of frozen embryos by embryonic stages such as late morula, early blastocyst and blastocyst were 37.5%, 50.0%o and 42.9%, respectively, and those of fresh embryos were 55.0℃, 64.3% and 69.2%, respectively. 3. When fresh and frozen embryos were transferred to recipients whose estrus were naturally induced, the pregnancy rates were 53.3% and 36.8%, respectively. However, the pregnancy rates were 65.7% and 66.0%, respectively when fresh and frozen embryos were transferred to recipients whose estrus were artificially induced by PGF₂α. 4. When fresh and frozen embryos were transferred into tip of uterine horn, the pregnancy rates were 77.4% and 72.4%, respectively. On the other hand, pregnancy rates were 31.3% and 21.4%, respectively when fresh and frozen embryos were transferred into the bottom of uterine horn.

엔지니어링 모델 Ka 대역 위성통신 탑재체 구현 및 시험

박종홍(J. H. Park),신동환(D. H. Shin),정병현(B. H. Chung),이재현(J. H. Lee),이성팔(S. P. Lee) 대한전자공학회 2000 대한전자공학회 학술대회 Vol.2000 No.11

Ka 대역 위성통신 중계기 개발

박종홍(J. H. Park),신동환(D. H. Shin),정병현(B. H. Chung),이성팔(S. P. Lee) 한국통신학회 1999 한국통신학회 학술대회논문집 Vol.1999 No.11

수정란이식에 의한 우 ( 牛 ) 의 쌍태유기에 관한 연구 1 . 성선자극호르몬의 투여에 대한 난소반응에 영향을 미치는 요인

정길생,이훈택,정병현,유승환,나진수 ( K . S . Chung,H . T . Lee,B . H . Chung,S . H . Yoo,J . S . Na ) 한국축산학회 1983 한국축산학회지 Vol.25 No.3

These experiments were carried out to obtain the fundemental information essential for the induction of superovulation in cattle. The effects of parity, season and the source of gonadotropins on ovarian response to gonadotropins given for superovulation were investigated. The results obtained in these experiments were summarized as follows: 1. Of 43 donor cattles treated with PMSG to induce superovulation, 86.0% responded with 2 or more corpora lutea and 55.8% with 6 to 13 corpora lutea. 2. No differences in ovarian response to PMSG administration among breeds were observed. 3. Average numbers of corpus luteum per donor cattle following PMSG administration in heifers, 1-2, 3-4 and 5 or more calved cows were 6.4, 9.3, 6.4 and 5.3, respectively. 4. No seasonal variations in ovarian response to PMSG given to induce superovulation were observed. 5. There were no significant differences in numbers of corpora lutea between the donor cattles treated with PMSG and FSH, respectively.

광대역 멀티미디어 서비스를 위한 실험용 Ka 대역 위성통신 탑재체 개발

박종흥,신동환,정병현,이성팔,Park, J.H.,Shin, D.H.,Chung, B.H.,Lee, S.P. 통신위성우주산업연구회 2000 Joint Conference on Satellite Communications Vol.2000 No.-

한국전자통신연구원은 국내 위성에 적용될 Ka 대역 위성통신 탑재체를 엔지니어링 모델로 개발하였다. 개발된 Ka 대역 탑재체는 대용량의 통신 서비스를 위한 200 MHz 채널 폭을 제공하며 대칭 및 비대칭 인터넷을 비롯하여 고품질의 멀티미디어 서비스와 155Mbps급 국간 중계서비스를 제공하는데 활용된다. 시스템 구성으로는 저잡음 증폭기-주파수 변환기, 채널 증폭기-고전력 증폭기로 구성되며, 다른 형태의 고전력 증폭기인 고체전력증폭기(SSPA)와 진행파관증폭기(TWTA)를 사용하여 시스템 구성에 유연성을 높였다. 구성품중 안테나, 저잡음증폭기,고체전력증폭기, 입력 광대역 여파기, 입.출력 채널 여파기는 ETRI에서 직접 개발하였다. 본논문은 이와 같01 구성한 Ka 대역 위성통신 탑재체의 구성부품 및 구조와 제작된 중계기에대한 RF 성능 시험 결과를 기술한 것이다. ETRI developed the engineering model Ka-band satellite communications system. This system is able to provide a high data rate communication service with a bandwidth of 200 MHz, a wideband digital trunking services up to 155 Mbps and symmetrical/asymmetrical internet multimedia services. The system employs the common receive/transmit antenna and the LNA-Downconverters. The different types of high power amplification, TWTA for one chain and SSPA for the other chain, are used for the variety of experimental options. The frequency selective surface type Antenna, MMIC LNA, MMIC SSPA, Input Filter, Input/Output Channel Filter developed by ETRI are verified via this system. This paper describes the results of the parametric RF test of this payload.

X - 정자와 Y - 정자의 분리에 관한 연구 1 . Sephadex Gel 여과에 의한 인간정자의 분리

엄기붕,정길생,정병현 ( K . B . Oum,K . S . Chung,B . H . Chung ) 한국축산학회 1986 한국축산학회지 Vol.28 No.12

These experiments were carried out to develop new techniques useful for in vitro separation of X- and Y-bearing spermatozoa. One ㎖ of human semen was applied to the column (14 × 1.06 ㎝) filled with swellen Sephadex G-25 fine or G-50 fine gel and then elutriated with Locke solution. Elutriated solution was fractionated into 1 ㎖ by automatic fraction collector and spermatozoa included in each fraction were subjected to the estimation of viability and recovery rate and to F-body test. The results obtained in these experiments were summarized as follows: 1. Few spermatozoa were visible in the 1st to 3rd fractions and the highest concentration of spermatozoa was obtained from 7th to 9th fractions. 2. In case of Sephadex G-25 fine, the viable rate of spermatozoa before and after chromatography was 81.1 and 83.3%, respectively and in case of Sephadex G-50 tine, it was 79.9 and 82.35%, respectively. 3. The recovery rates of sperm applied to Sephadex G-25 and 50 fine were 40.8% and 52.3%, respectively. 4. The percentage of spermatozoa without F-body was increased from 53.0 to 67.1% and 52.6 to 68.2, respectively by the filtration through Sephadex G-25 and 50 column.

韓國에서의 日本腦炎에 關한 硏究 - (第II報) 日本腦炎發生의 關係要因에 對한 醫學計學的 考察

Lee W.C.(李元暢),Yun K.B.(尹快炳),Lee T.J.(李澤柱),Yoon H.J.(尹和重),Chung, B.H.(鄭柄鉉),Lee S.M.(李相穆) 한국예방수의학회 1983 예방수의학회지 Vol.7 No.1

This paper will present an epidemiological information aspects of Japanese encephalitis in Korea of the past ten years from 1971 to 1980 and the medical statistical observation on the relative factor of Japanese encephalitis. It hoped that the information will be used for reference in future endeavors of study in the follows: The obtained results were as follows: 1. The mean of the incidence cases of Japanese encephalitis in Korea from 1971 to 1980 was 94.2±24.6(S.E.), that of incidence rate per 100,000 was 0.26±0.07 (S.E.) and that of case fatality rate was 3.6±0.89 per 100 cases. 2. The highest incidence cases of Japanese encephalitis could be found in younger whose age under 19 years old, which accounted 96% of the total incidence cases. 3. The correlation coefficient between the incidence cases of Japanese encephalitis and number of vaccinated in man was r=-0.416, its and pesticide consumtion amount was r=-0.432 and its and number of pig vaccinated was r=-0.485. 4. Correlation coefficient between year after imported and positive rate of HI test of Japanese encephalitis was r=+0.976.

抗卵丘細胞 抗體가 牛卵胞卵의 體外成熟과 受精 및 豚發達에 미치는 影響

鄭吉生,朴世必,鄭炯敏,嚴基鵬,李勳澤,鄭炳鉉 건국대학교 동물자원연구센터 1991 動物資源硏究誌 Vol.16 No.-

本 硏究에서는 卵巢內에 존재하는 다수의 未成熟 卵胞卵을 회수한 다음 이를 in vitro system에서 成熟, 受精 및 胚發達에 있어서 卵丘細胞(cumulus cell)가 미치는 영향과 그 기전을 조사하였다. 卵丘細胞에 대하여 좀더 구체적인 검토를 수행할 목적으로 牛卵胞卵에 대한 抗 卵丘細胞 抗體(Rabbit anti-bovine cumulus cell antibody)를 제조하여 Ammonium sulfate precipitation과 Protein A-sepharose CL-4B affinity chromatography의 의해 IgG만을 분리 정제한 다음, indirect ELISA와 Immunoprecipitation法에 의해 확인된 이들 각각의 抗體(intact cumulus cell antibody, solubilized cumulus cell antibody)를 牛卵胞卵의 體外成熟과 受精時 첨가하는 卵丘細胞가 體外成熟과 受精 및 갑發達에 미치는 영향을 조사하여 다음과 같은 결과를 얻었다. 1) 無處理 卵丘細胞와 溶解 卵丘細胞를 抗原으로 처리한 개체로부터 血淸을 채취하여 間接酸素免疫分析法으로 抗體 형성여부를 확인한 결과 1:12,800의 稀釋倍率에서도 두 抗血淸 處理區가 對照 血淸區에 비해 높은 O.D.價를 나타냄으로써 두 가지 형태의 抗原이 다같이 抗卵丘細胞 抗體를 생산하는 것으로 확인되었다. 2) Iodogn과 Immunoprecipitation를 이용하여 항체의 생성을 재차 確認하였는데 Iodogen method를 이용한 牛卵丘細胞의 ?? Iabelling은 25,000/10㎕ cmp이었다. 또한 ??-bovine cumulus cell과 각각의 抗血淸 反應 결과 생성된 Immunoprecipitates의 radioactivity와 O.D價를 조사하였던바, 無處理 혹은 溶解化된 卵丘細胞에서 공히 높은 抗體의 생성을 확인했으며 두 處理群을 비교해 볼 때 溶解化된 卵丘細胞 抗體가 無處理 卵丘細胞 抗體보다 더 높은 反應性과 O.D價를 가지고 있음을 알 수 있었다. 3) 無處理 卵丘細胞에 대한 抗血淸과 溶解化된 卵丘細胞에 대한 抗血淸으로부터 抗體를 분리해 냈을 때 정제된 Ig G의 O.D.價를 각각 1.19mg/ml 이상이었다. 4) 卵丘細胞가 부착된 卵胞卵과 제거된 卵胞卵에 無處理 卵丘細胞에 의해 생성된 抗體를 처리한 결과, Metaphase Ⅱ 段階에까지 발달한 卵胞卵은 卵丘細胞가 제거된 卵胞卵의 對照區를 포함한 모든 抗體 處理區의 경우 47.6∼59.1%로써 卵丘細胞가 부착된 卵胞卵의 對照區 78.8%에 비해 유의하게 낮았다. (P<0.01). 5) 卵丘細胞가 부착된 卵胞卵과 제거된 卵胞卵의 溶解 卵丘細胞에 의해 생성된 抗體를 처리한 결과, M Ⅱ 단계에까지 발달한 卵胞卵은 卵丘細胞가 제거된 卵胞卵의 對照區를 포함한 모든 抗體 處理區의 경우 47.6∼59.1%로써 卵丘細胞가 附着된 卵胞卵의 對照區 82.1%에 비해 有意하게 낮았다.(P<0.01). 抗卵丘細胞 抗體의 작용으로 인하여 이러한 卵丘細胞의 효과는 억제되는 결과로 미루어 볼 때 卵丘細胞는 體外成熟시 卵胞卵의 核成熟을 증진시키는 것으로 사료된다. 6) 卵丘細胞가 부착되었거나 또는 제거된 成熟 牛卵胞卵에 武處理 卵丘細胞에 대한 抗體를 처리한 다음 體外受精을 실시한 결과 卵丘細胞가 제거된 卵胞卵에 있어서 對照區를 포함한 모든 抗體 處理區의 受精率 45.0∼53.7%는 卵丘細胞가 부착된 卵胞卵에 있어서 對照區의 受精率 64.3%에 비해 다소 낮았다. 한편 雌性 및 雄性 前核이 동시에 형성된 牛卵胞卵의 비율은 卵丘細胞를 제거한 卵胞卵의 對照毆를 포함한 모든 抗體 處理區가 18.2∼38.9%로써 卵丘細胞가 부착된 卵胞卵에 있어서의 對照區 51.9%에 비해 有意하게 낮았다.(P<0.05). 7) 卵丘細胞가 부착되었거나 또는 제거된 成熟 卵胞卵에 溶解 卵丘細胞에 대한 抗體를 처리한 다음 體外受精을 실시한 결과 卵丘細胞가 제거된 卵胞卵에 있어서 對照區를 포함한 모든 抗體 處理區의 受精率 45.0∼52.5%는 卵丘細胞가 부착된 卵胞卵에 있어서 對照區의 受精率 62.8%에 비해 다소 낮았다. 또한 雌性 및 雄性 前核이 동시에 형성된 卵子의 비율은 卵丘細胞가 제거된 卵胞卵에 있어서의 對照區를 포함한 모든 부착된 卵胞卵가 26.3∼38.3%로써 卵丘細胞가 부착된 卵胞卵에 있어서의 對照區 55.6%에 비해 有意하게 낮았다.(P<0.05). 8) 抗體處理를 받은 牛卵胞卵의 체외발생을 조사하였던 바, 卵丘細胞가 제거된 卵胞卵에 있어서 對照區를 포함한 모든 抗體 處理區의 受精率이 8-細胞期, 16-細胞期 및 桑實胚期 혹은 胚盤胞期까지 발달한 비율은 7.1∼14.5, 2.9∼5.9 및 1.5%∼2.9%로써 卵丘細胞가 부착된 난자에 있어서 對照區의 18.6, 10.0 및 8.6%에 비해 낮은 胚發生率을 나타냈다. 抗卵丘細胞 抗體를 처리함으로써 卵丘細胞 작용이 억제된다는 本 硏究 결과는 卵丘細胞가 卵胞卵의 體外受精率과 前核形成率 및 胚發生能에 유의한 효과가 있다는 것을 시사한다. These experiments were carried out to investigate the effect of cumulus cells on in vitro maturation, fertilization, and subsequent development of follicular oocytes recovered from the bovine ovaries as well as to study the mechanisms involved in these processes. To examine the specific role of cumulus cell, antisera to bovine cumulus cell were produced Japanese Giant rabbit by repeated immunization of intact or solubilized bovine cumulus cell and purified IgG by the Ammonium sulfate precipitation and Protein A-Sepharose affinity chromatography. Then, these IgG of the bovine cumulus cell-specific antibodies were confirmed by indirect ELISA and Immunoprecipitation, and the effects of theses antibodies were investigated in vitro maturation, fertilizaiton, and development of bovine follicular oocytes. The results obtained in these experiment were summarized as follows: 1. The titer of the antibodies to cumulus cell determined by indirect ELISA using intact or solubilized bovine cumulus cell coasted plated was very high in both intact and solubilized cumulus cell. Especially, the optical density(O.D.) at 1: 12,800 dilution of antibodies was significantly higher than those of non-immunized control sera. 2. To reexamine the immunity of antibody, cumulus cells were labelled with I using the Iodogen method and the radioactivity of I into cumulus cell was shown approximately 25,000cpm/10㎕. The high radioactivity and O.D. value in immunoprecipitating reaction of I-bovine cumulus cell with anti-cumulus cell antisera were reconfirmed the existence of immuno-reactivity of antibody to cumulus cell. In addition, reactivities and O.D. values of solubilized cumulus cell antibodies were significantly higher than those of intact cumulus cell antibody. 3. O.D. values of the purified intact and solubilized cumulus cell antibodies were more than 1.19mg/ml. 4. When the follicular oocytes were treated with antibody to intact cumulus cell, the maturation rates of cumulus-enclosed and removed oocytes were ranged from 47.6 to 59.1%. These values are significantly lower than those(78.8%) fo follicular oocytes cultured without the antibody (p<0.01). 5. The maturation rates of cumulus-enclosed and removed oocytes treated with antibody against solubilized cumulus cell were ranged from 46.7 to 59.1%, showing significantly lower than those(82.1%) of oocytes cultured in antibody free medium(P<0.01). These results that the beneficial effect of cumulus cell to the oocyte maturation was inhibited by the action of antibody to cumulus cell suggest that cumulus cell enhances nuclear maturation of follicular oocytes. 6. When the follicular oocytes matured in vitro were treated with antibody to intact cumulus cell, the fertilization rates of cumulus intact and removed oocytes were ranged from 45.0 to 53.7%. These values are slightly lower than those(64.3%) of follicular oocytes not treated with the antibody, and increased frequencies of both male and female pronucler formations were detected in cumulus intact oocytes cultured in medium without antibody (P<0.05). 7. The fertilizaiton rates of cumulus intact and removed oocytes treated with antibody to solubilized cumulus cell were ranged from 45.0 to 52.5%, significantly lower than those(62.8%) of oocytes cultured in antibody free medium, and increased frequencies of ova with male and female pronuclei formations were found in the presence of cumulus cells(P<0.05). 8. The development rates of cumulus intact and removed oocytes to 8-, 16-cell, and morula or blastocyst stage after treatment of intact and solubilized cumulus cell antibody were ranged from 7.1 to 14.5 from 2.9, and from 1.5 to 2.9T, respectively. These rates were slightly lower than 18.6, 10.0 and 8.6% of cumulus intact oocytes cultured in medium without the antibody. The results that beneficial effects of cumulus cell to the pronuclear formation and embryo development were blocked by the action of antibody to cumulus cell suggest that cumulus cell significantly promotes normal fertilizaiton with proper pronuclear formation and embryo development.

우수 포유동물 수정란의 이용효율 제고에 관한 연구 : Ⅲ. 미세조작에 의한 수정란의 이용효율 증진에 관한 연구 Ⅲ. Study of Elevating Utilization Dfficiency of Embryos by Micromanipulation

정길생,김정익,김종배,정병현,이훈택,정형민 건국대학교 동물자원연구센터 2001 動物資源硏究誌 Vol.17 No.-

These experiments were carried out to apply the embryo manipulation technique, embryo bisection and blastomere separation, to animal industry. Bovine embryos were prepared through the in vitro maturation, fertilization, and development of follicular oocytes obtained from ovaries of slaughtered cow. The results obtained were summarized as follows: 1) Total 385 demi-embryos were produced from embryos by bisection and 90.4% of them were morphologically normal. 2) The viabilities of demi-embryos with or without zona pellucida in culture were 75.4~84.6% or 57.1~66.0%, respectively. 3) The developmental rates its demi-embryos of morulae or blastocysts were 77.6% and 68.2%, respectively. 4) After removing zona pellucida by pronase treatment, the rates of blastomere separation from 2-, 4-, and 8-cell stage embryos were 91.0, 75.8, and 63.2% respectively. After removing zona pellucida by micromanipulation, the rates of those were 81.5, 77.4, and 56.3%, respectively, showing the lower than those of pronase treatment. In addition, the better ratio were found in the ealier stage of embryos. 5) The developmental rate of blastomere to morula or blastocyst stage were 60.9~61.3% in 1/2 blastomeres, 57.1~59.3% in 1/4 blastomeres, and 41.7~50.7% in 2/8 blastomeres, showing the better rates in the earlier stage blastomeres. 6) After incubating 1/2, 1/4, and 2/8 blastomeres in ovine oviduct for 3~3.5 days, the recovery rates were 58.8, 58.5, and 29.6%, respectively, and the developmental rates to the morulae or blastocyst stage were 65.0, 58.3, and 37.5%, respectively.

優秀 愛玩犬의 效率的 增殖에 關한 硏究 : Ⅰ. 犬精液의 溫度變化가 精子性狀에 미치는 影響 Ⅰ. Effects of Temperature Changes on Spermatozoa Characteristicsin Canine Semen

李炳翰,崔錫和,李東熙,張京鎭,鄭柄鉉 건국대학교 동물자원연구센터 1992 動物資源硏究誌 Vol.17 No.-

This study was carried out to investigate the effects of temperature at handling procedures of collection, dilution and preservation of canine semen. The motility, progressive motility, velocity, and linearity of canine spermatozoa after diluted 1:1 with extenders at 35˚C were studied. The results were summarized as follows: 1. The motility, progressive motility, velocity, and linearity of the fresh spermatozoa were better at 39˚C than 25˚C or 15˚C (p<0.05). 2. The egg-yolk-tris (EYT) gave better than Cornell University extender(CUE) or Foote's extender (FE) in the motility, progressive motility, velocity, and linearity. 3. The motility, progressive motility, velocity, and linearity of spermatozoa in the semen diluted with EYT were better at 39˚C than 25˚C or 15˚C (p<0.05). 4. The motility of 70% was observed within 1.5 days in the EYT extender and it was better than other extenders. 5. The death rate of spermatozoa in CUE, FE, and EYT extenders was markedly increased between 2 and 2.5 days (p<0.01), and the death rate of 30% was observed between 1.5 and 2 days.