Antiinflammatory Effects of Tetracycline-loaded Biodegradable Membranes in Experimental Periodontitis of Beagle Dogs

전형식,설양조,박윤정,이용무,구영,류인철,이승진,한수부,최상묵,권수경,정종평,Jun, Hyung-Sik,Seol, Yang-Jo,Park, Yoon-Jeong,Lee, Yong-Moo,Ku, Young,Rhyu, In-Cheul,Lee, Seung-Jin,Han, Soo-Boo,Choi, Sang-Mook,Kwon, Soo-Kyoung,Chung, Chon The Korean Academy of Periodontoloy 2000 Journal of Periodontal & Implant Science Vol.30 No.3

조직유도재생술 과정에 사용된 차폐막의 술 후 오염 혹은 시술부위의 감염으로 조직재생유도가 제대로 이루어지지 않는 경우가 많이 있다. 테트라싸이클린은 넓은 범위의 치주 원인균에 효과적이고, 중성구 교원분해효소를 억제 함으로써 결합조직파괴를 억제하기 때문에 전신적 혹은 국소적으로 치주질환 치료에 널리 이용되어져왔다. 이번 연구의 목적은 비글견에 실험적으로 치주염을 유발시킨 후 테트라싸이클린 함유 차폐막을 이용하여 조직유도재생술을 하고서 테트라싸이클린의 유리반응을 관찰하는 것과 테트라싸이클린에 의한 항염, 항균효과를 알아보는 것이다. 실험 2-3개월 전에 비글견의 구강에서 치조골 결손부를 형성하여 치주염을 유발시켰다. 결손부 형성 2-3개월 후 실험군으로 테트라싸이클린 함유 차폐막으로 조직유도재생술을 하였고, 대조군으로 테트라 싸이클린이 함유되지 않은 차폐막으로 조직유도재생술을 하였고, 음성대조군으로 치은판막술만을 하였다. 시술 전과 시술 후 1, 2, 4주 간격으로 치은지수, 치태지수, 치은열구액의 양 및 혐기성 세균과 호기성 세균의 군락수를 측정하였다. 술 후, 1, 3, 5, 7, 및 14일 간격으로 유리된 테트라싸이클린의 농도를 측정하였다. 테트라싸이클린 함유 차폐막은 임상적 치은지수는 술 후 2, 4주째, 치태지수는 1, 2, 4주째 유의하게 감소하였다.(p<0.05) 1주에서 혐기성 및 호기성 세균집락수는 테트라싸이클린 함유 차폐막을 사용한 군이 약물이 함유되지 않은 차폐막보다 유의성있게 적었다.(p<0.001) 테트라싸이클린 함유 차폐막은 처음 1일은 높은 농도로 유리되었고 그 후 1주일 동안 일정하게 MIC 이상으로 유리되었다. 하지만 14일째는 유리가 관찰되지 않았다. 본 연구 결과, 테트라싸이클린 함유 생분해성 차폐막은 1주이상 약제가 MIC 이상 유리되었고, 유리된 테트라싸이클린에 의한 항염, 항균작용이 있어 치주조직 재생유도술시에 초기 치유가 잘 진행되도록 하는 효과가 있을 것이다.

염화 세틸피리디늄 분말의 치태제거와 치은염 완화 효과에 관한 임상시험

전형식,고영경,구영,류인철,최상목,정종평,Jun, Hyung-Sik,Ko, Young-Kyung,Ku, Young,Rhyu, In-Chul,Choi, Sang-Mook,Chung, Chong-Pyoung 대한치주과학회 1999 Journal of Periodontal & Implant Science Vol.29 No.1

To evaluate the effect of cetylpyridinium chloride in a powder form when used concomitantly with a commercial flouride containing tooth paste on inhibition of plaque formation, on gingivitis, and on irritation of oral mucosa, a double--blind, randomized parallel study was set up. Cetylpyridinium chloride was incorporated into a ligh brown colored powder with menthol added for scent. There were no diffemces between the active agent and the placebo which did not contain cetylpyridinium chloride in appearance, color, smell, taste, or dispenser. 98 healthy volunteers were assigned to one of two groups: brushing 3 times a day with flouride toothpaste and cetylpyridinium chloride powder, or brushing 3 times a day with flouride toothpaste and placebo. Before the test period, the subjects received through tooth cleaning and polishing. At basteline, GI, PI, BOP, and GCF of the Ramfjord teeth were measured in the experimental and placebo groups including 58 and 42 subjects repectively, After 4 weeks, GI, PI, BOP, GCF, compliance, irritation of the oral mucosa(redness, pus drainage, edema) and adverse reactions were measured. The PI, GI, and BOP of the experimental group recorded at baseline are 0.19${\pm}$0.19, 0.42${\pm}$0.31, and 0.08${\pm}$0.15. These scores showed significant decrease after 4 weeks of test period(0.11${\pm}$0.15, 0.22${\pm}$0.24, 0.02${\pm}$0.09 repectively at the end of the study) and inhibition of plaque accumulation and resolution of gingival inflammation could be observed. GCF shoed slight increase but this was not statistically different. Comparison of changes in measured scores of control and experimental groupshow GI, PI of the test indices have decreased. Test group showed significantly greater decrease in gingivitis and plaque accumulation after 4 weeks. GCF and BOP also showed greater decrease thant the control group but this difference was not statistically significant. At no time of the study period did any of the subjects show signs of irritation of the oral mucosa or adverse reactions. Following conclusions could be obtained from this study. The combined use of cetl powder and flouride toothpaste showed greater inhibition of plaque accumulation and greater decrease of gingivitis than use of flouride toothpaste with placebo agent.

식도정맥류환자에서의 (食道靜脈瘤患者) 식도운동에 (食道運動) 관한 연구

김정용(Chung Young Kim),송인성(In Sung Song),최규완(Kyoo Wan Choi),정영화(Young Hwa Chung),이영상(Yung Sang Lee),전형식(Hyung Sik Jun) 대한소화기학회 1984 대한소화기학회지 Vol.16 No.2

N/A Esophageal motility was studied in 14 control cases and 14 cases with esophageal varix, using low compliance pneumohydraulic capillary infusion system. 1) There was no difference in lower esophageal sphincter pressure between control and varix group (20.0+-l8.3mmHg and 19.8+- 5.7mmHg, respectively), but low esophageal peristaltic pressures were decr eased in varix group (51.0+-34.1mmHg) comparing with those in control group. (76.4+-16.4mmHg) (p<0. 05). The decrease of low esophageal peristaltic presure in varix group had no relation with varix grade or age distribution. 2) The lower esophageal peristaltic pressures were increased significantly after metoclopropamide administration in control (from 76.4+-16.4mmHg to 101.2+-21.6mmHg) and varix group (from 51.0+-34.1mmHg to 70.1>38.6mmHg), but the change of lower esophageal sphincter pressure was not significant.

복수와 흉막 삼출액이 합병된 장티푸스간염 2예

추현광,신언수,오세진,황일순,김주현,박현철,전형식 대한감염학회 1994 감염 Vol.26 No.4

저자들은 장티푸스에 흉수와 복수가 합병된 2예의 환자중 한명의 환자 복수에서 Salmonella typhi가 배양된 경우를 치료한 경험을 하였기에 문헌 고찰과 함께 보고한다. Typhoid fever is an acute systemic febrile disease caused by Salmonella typhi. Salmonella infection involves a variety of multiple organs. But ascites with pleural effusion has rarely been reported to occur as a complication of typhoid fever since the medical use of chloramphenicol in Korea. We report two cases of typhoid fever complicated with ascites and pleural effusion and in one of two cases S. typhi was isolated in ascitic fluid.

간세포암의 간동맥 화학색전술 후 합병된 간농양 1예

추현광,김주현,오세진,박혜영,박현철,전형식 대한감염학회 1995 감염 Vol.27 No.1

저자들은 간세포암 환자에서 간동맥 화학색전술 시행 후 합병된 Enterobacter cloacae 에 의한 간농양 1예를 경험하였기에 문헌고찰과 함께 보고한다. Transcatheter arterial chemoembolizaion(TACE) is a useful alternative to surgery and systemic chemotherapy for inoperable hepatocellular carcinoma. The common adverse reaction observed following TACE is a postembolization syndrome which consists of abdominal pain, fever, nausea, vomiting and leukocytosis. Heapatic abscess and septicemia have been reported to be rare complications. We experienced a case of hepatic abscess following TACE for a huge hepatocellular carcinoma. Enterobacter cloacae was isolated from drainage of the abscess and the patient was recovered with antimicrobial therapy and percutaneous drainage of abscess.

SV40 Large T 유전자를 이입해 불멸화시킨 사람 간근섬유아양 세포주의 확립

이효석,김정룡,윤정환,이준성,우광훈,전형식 대한소화기학회 1999 대한소화기학회지 Vol.34 No.3

Background/Aims: Myofibroblast like cell (MFLC) plays an important role in hepatic fibrogenesis. A continuously proliferating human MFLC line would be valuable in studying human hepatic fibrogenesis. The present study was attempted to establish immortalized human liver MFLC line. Methods: Fetal liver cells were transfected with SV40 large T gene. The transfected cells were selected and those colonies with morphologic characteristics of MFLCs were further cloned by limiting dilution method. Growth characteristics of subcultured cells were investigated. Indirect immuno-fluorescece studies were carried out to confirm the expression of SV40 large T antigen, to analyze the expression pattern of intermediate filaments, and to detect smooth muscle alpha-actin. Moreover, the activity to store vitamin A was analyzed. Results: Subcultured cells were spindle in shape and aggregated to form a typical hills-and-valleys structure in an overconfluent culture condition. They were stained positively for SV40 large T antigen. Doubling time of the cells was approximately 36 hours. Their growth rates were accelerated by increasing serum concentration in the media and they did not form colonies in soft agar assay. They were stained to vimentin and smooth muscle alpha-actin but not to cytokeratin and factor-VIII related antigen. These cells were also able to take up and store vitamin A. Conclusions: We established an immortalized human MFLC line derived from the hepatic stellate cells.

식도 궤양, 급성 담낭염 및 허혈성 대장염의 소견이 현저한 알레르기성 육아 종증(Churg-Strauss Syndrome) 1 예

김광호,박성혜,이상헌,김주현,박현철,남상민,정숙향,김호연,황일순,전형식,추현광 대한내과학회 1993 대한내과학회지 Vol.45 No.3

Churg-Strauss syndrome is a kind of rare systemic vasculitis characterized by peripheral eosinophilia, bronchial asthma and relatively specific pathological findings such as necrotizing vasculitis, eosinophilic infiltration of tissue and/or extravascular granuloma. Clinically multiple organs/systems can be involved with various manifestations of disease of lung, heart, skin, musculoskeletal system, nervous system and gastrointesinal/hepatobiliary tract. Gastrointestinal symtoms were frequently accompanied, but usually mild and easily controllable so that special study for evaluation of the symptoms hardly seemed to be needed. We experienced a case of Churg-Strauss syndrome presenting as esophageal ulcers, acute acalculous cholecystitis and treatment-resistant ischemic colitis in a 24-year-old male patient with acute upper abdominal pain and watery, often bloody diarrhea. He also showed peripheral eosinophilia, bronchial asthma, mononeuritis multiplex, skin rash and arthritis. The patient received high dose corticosteroid, cyclophosphamide and exchange plasmapheresis with resolution of almost all symptoms. But symptoms of ischemic colitis such as abdominal pain and diarrhea are not completely improved in spite of intensive therapy.