Methy1 jasmonate 처리에 의한 인삼 (Panax ginseng C.A Meyer) 부정근의 이차대사산물 및 항산화활성 증가

임순,신차균,김윤수,배기화,김윤영 한국식물생명공학회 2005 JOURNAL OF PLANT BIOTECHNOLOGY Vol.32 No.3

This study was initiated to investigate the impacts of methyl jasmonate (MeJA) on adventitious rootgrowth of Panax ginseng, the production of secondary metabolites, such as ginsenosides and phenolic compounds, and antioxidative activity. Among various concentrations of MeJA, 100 μM MeJA increased the ginsenosides accumulation to 26.6 mg/g dry wt, about 8 times higher than the control in ginseng adventitious roots (GAR). In addition, 50 μM MeJA increased the accumulation of phenolic compounds to 0.38 mg/g dry wt, about 3 times higher than control in GAR. This Me(CM) which obtained in bioreactor after 40 days of culture than in fresh medium (FM). Treatment of 100 M MeJA in CM increased the accumulation of ginsenosides (1.7 times) and phenolic compounds (1.2 times) more than in FM, respectively. Consequently, these high accumulation of ginsenosides and phenolic compounds by MeJA led to increase the antioxidative activities expressed to the DPPH scavenging activity (over 78.3%). The DPPH scavenging activity in control was 45.5%. 인삼부정근 배양에서 MeJA 처리가 부정근내 진세노사이드와 페놀화합물의 생산에 미치는 영향과 이러한 이차대사산물의 증가에 따른 인삼부정근의 항산화활성의 효과를 조사하였다. 다양한 농도의 MeJA를 인삼부정근에 처리한 결과, 100 μM MeJA에서 부정근내 진세노사이드의 생산은 26.6 mg/g dry wt로 대조구보다 약 8배 이상 생산하는 것으로 나타났다. 하지만, MeJA의 처리는 부정근의 생장을 감 소시키는 것으로 나타났다. 페놀화합물의 생산 역시 MeJA 처리에 의하여 증가되는 경향을 나타냈으며 50 μM MeJA에서 0.38 mg/g dry wt로 대조구에 비하여 약 3배 이상 증가한 것으로 나타났다. 이러한 MeJA의 처리효과는 조건배 지 (CM)를 이용하는 것이 신선배지 (FM)를 이용하는 것보다 유리한 것으로 나타났다. 배지성분이 결핍된 조건배지에서의 진세노사이드 생산은 신선배지에서 보다 약 1.7배가 증가한 것으로 나타났으며, 페놀화합물 역시 조건배지에서 약 1.2배가 증가한 것으로 나타났다. 이러한 MeJA 처리에 의한 진세노사이드와 페놀화합물의 증가는 결과적으로 인삼부정근의 항상화활성을 무처리구인 대조구에 비해서 약 72%이상 증가시키는 것으로 나타났다.

Agrobacterium 매개에 의한 고구마 형질전환 및 식물체 재분화

임순,양경실,권석윤,백기엽,곽상수,이행순 한국식물생명공학회 2004 JOURNAL OF PLANT BIOTECHNOLOGY Vol.31 No.4

Transformed sweetpotato (Ipomoea batatas (L.) Lam. cv. Yulmi) plants were developed from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain EHA105/ pCAMBIA2301 harboring genes for intron -glucuronidase (GUS) and kanamycin resistance. Transient expression of GUS gene was found to be higher when embryogenic calli were co-cultivated with Agrobacterium for 2 days. The co-cultured embryogenic calli transferred to selective MS medium containing 1 mg/L 2,4-D, 100 mg/L kanamycin, and 400 mg/L claforan. These embryogenic calli were subcultured to the same selection medium at 4 weeks interval. Kanamycin-resistant calli transferred to hormone-free MS medium with kanamycin gave rise to somatic embryos and then converted into plantlets in the same medium. Southern blot analysis confirmed that the GUS gene was inserted into the genome of the sweetpotato plants. A histochemical assay revealed that the GUS gene was preferentially expressed in the leaf, petiole, and vascular tissue and tip of root. 국내 고구마 율미 품종의 배발생 캘러스를 Agrobacterium 매개 방법을 이용하여 형질전환 식물체를 개발하였다. 배발생 캘러스를 7일 동안 전배양 한 후 Agrobacterium과 2일간 공동배양할 경우 일시적인 형질전환 효율이 가장 높았다. Agrobacterium과의 공동배양 후 배발생 캘러스를 1 mg/L 2,4-D, 100 mg/L kanamycin, 400 mg/L claforan이 첨가된 선발배지에서 4주 간격으로 계대배양하였다. 선발된 kanamycin 저항성 캘러스를 2,4-D를 제거한 선발배지로 옮겨 체세포배를 유도하였으며 이후 소식물체로 발달하였다. Southern 분석으로 1-3 copy의 GUS 유전자가 고구마 염색체내로 도입되었음을 확인하였다. 또한 조직학적 분석으로 GUS 유전자가 형질전환 고구마의 배발생 캘러스, 재분화 식물체의 잎, 엽병, 및 뿌리 조직에서 강하게 발현됨을 알 수 있었다.

한국 속옷의 경쟁력 강화를 위한 착용감 및 착용방법에 관한 연구

임순 복식문화학회 1997 학술발표회 Vol.1997 No.-

韓國女性의 體表面積에 關한 硏究(第1報) : 體表面積 및 그의 按分比率에 대하여 Body Surface Area and It's Rational Rate

林珣,車玉善 한국의류학회 1988 한국의류학회지 Vol.12 No.3

The purpose of study was to measure to body Surface area and the rational rate of Korean adult's women. The subjects are 20 years old to 49 years old women (Their bust width ranges from 74.5 to 101. 5 cm, the height from 144.6 to 163. 3cm, the weight from 44. 2 to 74.0 kg, Rohrer Index from 1. 02 to 1.89). As the experimental method, both the gypsum method, by which the shape of body can be copied as it is, and weighing method, from which planed body surface area can be measured with consistant thickness of polypropylene film used. The results were obtained as follows. 1. After dividing the Korean adult's women into four groups (single and married women in their twenties, those in their thirties, those in their fourties). The change of body surface area was reviewed in accordance with age groups. No great difference among age group was showed in whole body surface area. 2. The average value of body surface area is 1, 514m₂ and the part of trunk shows the biggest difference to the age groups. 3. After dividing the middle of body into two groups, the relation of symmetry of each parts is reviewed with difference in body surface area. The results shows that the part of head & neck and lower limbs are symmetrical. The part of trunk and upper limbs are unsymmetrical. 4. Regional rates of each part of whole body surface area are follows. head 4.98%, each-lobes 0.46%, neck 2.01%, face2.48%, upper trunk 19.64%, lower trunk 13. 91%, upper armpits 1.76%, lower armpits 12.52%, , hands 4.64%, thighs 18. 89%, lower legsl 3.10%, feet 6.01%.

비타민 E 섬유가 인체생리 반응에 미치는 영향

임순,정명희 복식문화학회 2004 춘계학술대회 Vol.2004 No.-

체표면적 산출식에 관한 연구 : 40대 여성을 중심으로 Centering around 40 ages

임순 服飾文化學會 1994 服飾文化硏究 Vol.2 No.2

Data of the body surface is a necessary unit for the measuring of metabolism energy and activity energy. And also, these data are referring to check the degree of retaining warmth of clothes, to find the effect of hear insulation according to body surface, to calculate an average temperature of skin, and to study the several fields of clothing. In measuring of body surface, it is actually impossible to measure a subject's body surface in each experiment. As the experimental method, both gypsum method, by which the shape of body can be copied as it is, and the weighing method from which planed body surface area can be measured with consistant thickness of polyprophylene film as used. In fact, every female subject feels uncomfortable to measure her body surface as a naked body.

Methyl jasmonate 처리에 의한 인삼 (Panax ginseng C.A. Meyer) 부정근의 이차대사산물 및 항산화활성 증가

임순,배기화,신차균,김윤영,김윤수,Lim Soon,Bae Ki-Hwa,Shin Cha-Gyun,Kim Yoon-Young,Kim Yun-Soo 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.3

인삼부정근 배양에서 MeJA 처리가 부정근내 진세노사이드와 페놀화합물의 생산에 미치는 영향과 이러한 이차대사 산물의 증가에 따른 인삼부정근의 항산화활성의 효과를 조사하였다. 다양한 농도의 MeJA를 인삼부정근에 처리한 결과, $100\;{\mu}M$ MeJA에서 부정근내 진세노사이드의 생산은 26.6 mg/g dry wt로 대조구보다 약 8배 이상 생산하는 것으로 나타났다. 하지만, MeJA의 처리는 부정근의 생장을 감소시키는 것으로 나타났다. 페놀화합물의 생산 역시 MeJA처리에 의하여 증가되는 경향을 나타냈으며 $50\;{\mu}M$ MeJA에서 0.38 mg/g dry wt로 대조구에 비하여 약 3배 이상 증가한 것으로 나타났다. 이러한 MeJA의 처리효과는 조건배지 (CM)를 이용하는 것이 신선배지 (FM)를 이용하는 것보다 유리한 것으로 나타났다. 배지성분이 결핍된 조건배지에서의 진세노사이드 생산은 신선배지에서 보다 약 1.7배가 증가한 것으로 나타났으며, 페놀화합물 역시 조건배지에서약 1.2배가 증가한 것으로 나타났다. 이러한 MeJA 처리에 의한 진세노사이드와 페놀화합물의 증가는 결과적으로 인삼부정근의 항상화활성을 무처리구인 대조구에 비해서 약 72%이상 증가시키는 것으로 나타났다. This study was initiated to investigate the impacts of methyl jasmonate (MeJA) on adventitious root growth of Panax ginseng, the production of secondary metabolites, such as ginsenosides and phenolic compounds, and antioxidative activity. Among various concentrations of MeJA, $100\;{\mu}M$ MeJA increased the ginsenosides accumulation to 26.6 mg/g dry wt, about 8 times higher than the control in ginseng adventitious roots (GAR). In addition, $50\;{\mu}M$ MeJA increased the accumulation of phenolic compounds to 0.38 mg/g dry wt, about 3 times higher than control in GAR. This MeJA treatment was more effective in conditioned medium (CM) which obtained in bioreactor after 40 days of culture than in fresh medium (FM). Treatment of $100\;{\mu}M$ MeJA in CM increased the accumulation of ginsenosides (1.7 times) and phenolic compounds (1.2 times) more than in FM, respectively. Consequently, these high accumulation of ginsenosides and phenolic compounds by MeJA led to increase the antioxidative activities expressed to the DPPH scavenging activity (over $78.3\%$). The DPPH scavenging activity in control was $45.5\%$.

20대 여성을 위한 새로운 체표면적 산출식에 관한 연구

林珣 服飾文化學會 1999 服飾文化硏究 Vol.7 No.2

The purpose of this study is providing a simple, relatively errorless body surface area calculation. Subjects were 10 married women and 10 singles women whose age was 20 to 29 years old. The Gypsum method has applied for the sampling of body surface. The Weight method has been used to measure body surface by means of transferring gypsum shape on uniform plane polypropylene films. In this study, compare analyzed errors between the traditional formulas for measuring body surface area and measuring data in this experiment. More than all, it has been to induce a regression equation for measuring body surface area, which is so simple to calculate with less errors, with variable factors as weight and height. The results of this experiment as follows: 1. In the traditional formulas, weight formula was shown high average error: Niya's height formula, which was modified K value as 0.62 in the height formula (S=KH) is shown lower average error than Lassabilere's Height formula. 2. In the weight-height formula (S=K WH), it was shown high average error according to the increasing of K value. Kawanami's formula, which 5.378 as K value, was shown low average error both the singles and the married women.

Agrobacterium 매개에 의한 고구마 형질전환 및 식물체 재분화

임순,양경실,권석윤,백기엽,곽상수,이행순,Lim, Soon,Yang, Kyoung-Sil,Kwon, Suk-Yoon,Paek, Kee-Yoeup,Kwak, Sang-Soo,Lee, Haeng-Soon 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.4

Transformed sweetpotato (Ipomoea batatas (L.) Lam. cv. Yulmi) plants were developed from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain EHA105/pCAMBIA2301 harboring genes for intron $\beta$-glucuronidase (GUS) and kanamycin resistance. Transient expression of GUS gene was found to be higher when embryogenic calli were co-cultivated with Agrobacterium for 2 days. The co-cultured embryogenic calli transferred to selective MS medium containing 1mg/L 2,4-D, 100mg/L kanamycin, and 400mg/L claforan. These embryogenic calli were subcultured to the same selection medium at 4 weeks interval. Kanamycin-resistant calli transferred to hormone-free MS medium with kanamycin gave rise to somatic embryos and then converted into plantlets in the same medium. Southern blot analysis confirmed that the GUS gene was inserted into the genome of the sweetpotato plants. A histochemical assay revealed that the GUS gene was preferentially expressed in the leaf, petiole, and vascular tissue and tip of root. 국내 고구마 율미 품종의 배발생 캘러스를 Agrobacterium 매개 방법을 이용하여 형질전환 식물체를 개발하였다. 배발생 캘러스를 7일 동안 전배양 한 후 Agrobacterium과 2일 간 공동배양할 경우 일시적인 형질전환 효율이 가장 높았다. Agrobacterium과의 공동배양 후 배발생 캘러스를 1mg/L 2,4-D, 100mg/L kanamycin, 400mg/L claforan 이 첨가된 선발배지에서 4주 간격으로 계대배양하였다. 선발된 kanamycin 저항성 캘러스를 2,4-D를 제거한 선발배지로 옮겨 체세포배를 유도하였으며 이후 소식물체로 발달하였다. Southern 분석으로 1-3 copy의 GUS 유전자가 고구마 염색체내로 도입되었음을 확인하였다. 또한 조직학적 분석으로 GUS 유전자가 형질전환 고구마의 배발생 캘러스, 재분화 식물체의 잎, 엽병, 및 뿌리 조직에서 강하게 발현됨을 알 수 있었다.

Under-Wear의 착용감과 착용방법에 관한 연구

林珣 服飾文化學會 1998 服飾文化硏究 Vol.6 No.4

This paper is based on a survey of consumers' opinions on underwears. The questions in the survey dealt with what materials were used to manufacture under wears, which underwears were worn together in combinations, and how comfortable underwears were, In addition, the respondents were asked about how many pieces of different underwears they possess. Through such research, this paper aims to provide a guide to manufacturing domestic underwears that are competitive against foreign brands. 336 women in their twenties, thirties, forties and fifties and fifties from Seoul and it's suburbs were surbeyed. Statistical analysis was performed by calculating the average, frequency and standard deviation, and through one-way analysis and the Duncan test. The following are the results of the study: 1. Consumers were moderately satisfied with Korean-made underwears, In order to compete with foreign brands, products with hi호 consumer-satisfaction levels must be produced. 2. Upper garments usually consisted of brassieres with shoulder straps and sleeveless shirts. For lower underwears, most people tended to wear panties under girdle, with garlets on top, This is most likely the result of more people wearing slacks than skirts. 3. Consumer in their twenties had an average of 1.71 slips, while those in their thirties had 3.07, those in their forties had 3.25, and people in their fifties had 3.88 slips. Thus the number of slips a consumer possesses increases as age increases. For socks, people in their twenties had 7.4 pairs on average, those in their thirties had 5.5, those in their forties had 6.7, and those in their fifties had 5.3, Thus, those in their twenties possessed the most number of socks. Consumers in their twenties also had the largest proportion of socks among all the types of hosiery combined, with 47.1% of their hosiery being socks. 4. Consumers did tend to prefer cotton underwears, but according to the type of underwears, products using mixed fabrics of cotton and synthetic fibers were also frequently worn. 5. The shape and form of underwear that consumers perfer in a certain time frame varies according to the latest fashion in outer wear. Therefore, planning design of underwear products by predicting the future trend of outer wear fashion is necessary.