국내 거베라 육종계통 형질전환 기초 조건 확립

이혜영,이기정,전은희,신상현,이재헌,김도훈,정대수,정용모,조용조,김정국,정영수,Lee, Hye-Young,Lee, Ki-Jung,Jeon, Eun-Hee,Shin, Sang-Hyun,Lee, Jai-Heon,Kim, Doh-Hoon,Chung, Dae-Soo,Chung, Yong-Mo,Cho, Yong-Cho,Kim, Jeong-Kook,Chung, You 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.1

본 연구에서는 거베라 형질전환 기초기술개발을 위한 연구를 수행하였다. 경남원예화훼시험장에서 육성된 국내 거베라 18품종 중, Agrobacferium 감염에 순응적인 품종을 선발하기 위하여 18개의 국내 거베라 품종을 스크린하여 011, 016, GM023 등 12개의 순응형 계통을 스크린 하였으며 효율적인 형질전환을 위한 제균 항생제로는 cefotaxime이 선택되었다. 더 많은 캘러스 유도를 위한 전처리 배지의 호르몬 농도는 BA 2 ppm, Zeatin 2 ppm, IAA 0.2 ppm (2 x media)이 효율적이었고 재분화 배지의 호르몬 농도도 전처리 배지 호르몬의 농도와 동일하게 BA 2 ppm, Zeatin 2 ppm, IAA 0.2 ppm (2 x media)에서 효율적이었으나 GUS 발현에는 다른 차이가 없었다. 고효율 형질전환 체계 확립을 위하여 네 가지 다른 전처리 기간 처리와 두 가지 다른 Agrobacterium tumerfaciens 사용, 두 가지 다른 공배양 기간을 처리한 결과 전처리 하지 않은 엽병과 엽신을 LBA4404 Agrobacterium tumerfaciens을 사용하여 5일 간 공배양 기간을 가졌을 때와 dipping 처리하여 접종한 엽병과 엽신에서 높은 GUS 발현빈도를 나타냈다. Gerber (Gerbera hybrida) is a valuable ornamental species grown as a potted plant and cut flowers. However, genetic variability within the gerbera genus is very limited. So it is absolutely needed to introduce and widen genetic resources into gerbera lines by genetic transformation. For the purpose, 18 Korean gerbera lines were screened to establish Agrobacterium-mediated genetic transformation procedure. In an experiment to select Korean gerbera lines which are amenable to Agrobacterium-inoculation, 12 lines turned out to be positive in Agrobacterium-inoculation. More callus were produced from BA 2ppm, Zeatin 2ppm, IAA 0.2ppm in pre-culture and regeneration medium (2X media) but there was no difference in the frequency of GUS expression rate. In another experiment to find out optimal condition for highly efficient Agrobacterium-inoculation, petiole and leaf explants have been treated with four different pre-culture periods, two different co-culture periods and two different Agrobacterium strains. As a result, high GUS expression has been showed from petiole and leaf explants treated no pre-culture period with LBA4404 Agrobacterium tumerfaciens, 5 day co-culture period and dipping treatment.

Design and Simulation of a Silicon Photomultiplier Array for Space Experiments

이혜영,남신우,박일흥,J. Lee,김지은,박재형 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.2

A new type of photon sensor known as the silicon photomultiplier (SiPM) is a matrix of tiny avalanche photodiodes operating in the Geiger mode. The output of a SiPM sensor is the sum of signals from all `binary' diodes of the sensor, providing the capability for single-photon counting when illuminated with a low intensity of photons. All the features of the SiPM provide a promising photon sensor for space applications that require low power, small size and weight, less sensitivity to a magnetic field, {\it etc}. For the image sensor to be applied in a future space telescope, we have designed a 4 × 4 array of SiPM sensors, each consisting of 32 × 32 photodiode micropixels with a size of 30 × 30 μm². The design scheme and a simulation of the performance are presented.

국내 학술지 논문의 주제어를 통한 학술연구분야 관계분석

이혜영,곽승진,Lee, Hye-Young,Kwak, Seung-Jin 한국비블리아학회 2011 한국비블리아학회지 Vol.22 No.3

본 연구의 목적은 국내 연구자들의 연구 성과물인 학술지 논문에 나타난 학문분야의 상호관계성과 연관성에 대한 연구로 연구동향에 대한 시사점을 제시하는 것이다. 학술논문을 이용한 계량적 연구는 주로 논문의 초록, 자동추출 키워드, 논문의 풀텍스트를 활용하는 것이 일반적이나 본 연구는 실험대상 학술논문의 저자가 제시한 주제어에 초점을 두었다. 실험대상 자료는 2009년도 한국연구재단 등재학술지 80종으로 학술논문 7,616건, 주제어 58,143건이다. 자료 분석결과 공학, 농수해양, 복합학, 사회, 예술체육, 의약학, 인문, 자연과학 등의 8개 각 연구분야는 주제어 사용에서 차이점을 보였다. 특히 공학분야의 주제어가 다른 연구 분야에서도 주제어로 많이 사용되고 있었다. 또한 주제어 네트워크를 통해 연구분야를 클러스터링 한 결과, 3개 클러스터를 얻을 수 있었다. 클러스터A는 '공학', '자연과학', '사회', '복합학', '인문', 클러스터B는 '의약학', '예술체육', 클러스터C는 '농수해양'이었다. The purpose of this paper is to analyze the interrelation among research areas based on domestic journal papers, achievements of korea researchers. Generally, the content of papers is appeared through abstracts, subjects, full-text and so on. This paper is focused on subject terms of Domestic journal papers. The experimental data are 80 domestic journals, 7,616 papers and 58,143 subject terms and papers published in 2009. As the result, it was different to use subject terms on each research area: Engineering, Agriculture & Oceanography, Interdisciplinary Science, Social Science, Arts & Physical Education, Medicine & Pharmacology, Humanities and Natural Science. Subject terms of Engineering have used the most in the other research areas in aspect of term co-occurrence. The 8 research areas were grouped in 3 clusters: C1(Engineering, Natural Science, Social Science, Interdisciplinary Science, Humanities), C2(Medicine & Pharmacology, Arts & Physical Education), and C3(Agriculture & Oceanography).

갈겨니(Zacco temminki)의 진화에 관한 연구 VII.갈겨니 2 Type의 Mitochondrial DNA변이

이혜영,양서영,백상기,박창신,유성림,이성근,Lee, Hei-Yung,Yang, Suh-Yung,Paik, Sang-Gi,Park, Chang-Shin,Yu, Sung-Lim,Lee, Sung-Keun 한국통합생물학회 1988 동물학회지 Vol.31 No.3

한국산 담수 어류인 갈겨니(Zacco temminki)는 형태적으로 동일하거나 전기영동법에 의한 s-Mdh에 2type(MM과 MS type)이 있음을 (1987)이 주장하였으며 2type간 유전적 차이 정도를 분석한 결과 자매종으로 밝힌바 있다. 본 연구에서는 상기 결과를 토대로 2type의 mtDNA를 4개 집단에서 추출하여 11가지 제한 요소로 처리한 다음 fragment양상을 비교 분석하였다. 갈겨니 mtDNA의 전 genome크기는 약 16.7Kb였으며 frabment homolgy(F)에서 MS type 간 F값은 0.464,MM type간 F값은 0.762로 높은 값을 나타냈다. 또한 MM과 MS 이형간 평균 F값은 0.312(0.258-0.345범위)로 동형간보다 낮은 유사성을 나타내고 있다. 집단 또는 type간 nucleotide sequence divergence(p)를 산출한 결과 MS type 간의 P=0.128에 비해 동형간의 P값은 0.045로 매우 낮은 mtDNA sequece 변이를 나타내었다. 그러나 이형간인 2type평균 P값은 0.195(0.177-0.226범위)로 차이를 나타냈다. 이와 같은 2type간의 차이는 isozyme연구 분석 결과와 일치하고 있어 갈겨니 2type간의 종분화를 확신시키는 새로운 자료가 되었다. Mitochondrial DNAs of two Mdh allelotypes of the dark chub, Z. temmincki inhabiting in Korean fresh water, were analysed. Samples of each type were collected from four populations, and the fragment patterns for mtdNA of each type were explained from 7 of the eleven restriction enzymes with hexanucleotide recognition site. Genome size was approximately 16.7 kilobases. The highly typical mtdNA fragments of each type were discovered in digestion profiles produced by Eco RI and Pst I enzyrnes. The comparisons of restriction fragment patterns and relative digestion maps permitted the estimation of fragment homology (F) and nucleotide sequence divergence(p). Between the two identical types, sequence divergence(p) was 0.128(MS), and 0.045(MM), ; between the two different types, 0.195 (range 0.177-0.226). These result may provide a distinct difference more than the value derived from allozyrne analysis, and a powerful new molecular approach for assessing genetic-evolutionary relationship among fishes.

국제 표준화회의 참가보고 - 3GPP 제85차 RAN4 및 제124차 SA2 국제회의

이혜영,임한나,Lee, Hye-Yeong,Im, Han-Na 한국정보통신기술협회 2018 TTA저널 Vol.2018 No.-

고정성 국부의치 die Trimming의 기술

이혜영,Lee, Hye-Yeong 대한치과기공학회 1980 대한치과기공학회지 Vol.2 No.1

뇌성마비아동 어머니의 스트레스 및 대처방식 조사

이혜영,김경,차용준,Lee, Hye-Young,Kim, Kyoung,Cha, Yong-Jun 대한물리치료학회 2012 대한물리치료학회지 Vol.24 No.2

Purpose: The goal of this study was to analyze the degree of stress and methods of coping with stress in mothers of cerebral palsied children and to provide a basic resource for the development of policies to improve the mental health of mothers with cerebral palsied children. Methods: 85 mothers with cerebral palsied children and 77 mothers of normal children completed a self-administered questionnaire that evaluated the degree of stress and methods of coping with stress. Cronbach's ${\alpha}$ score was used to determine the internal consistency of the acquired data and the discriminated validity was estimated by Pearson's correlation coefficient. Independent t-tests were conducted to compare the degree of stress and method of coping with stress between mothers of cerebral palsied children and mothers of normal children and one-way ANOVA was performed to analyze the effect of generalized characteristics on stress in mothers of cerebral palsied children. Results: The degree of stress in terms of anxiety response and roles as mother for mothers with cerebral palsied children was higher than mothers with normal children. However, there was no significant difference between two group in regards to the methods of coping with stress during a stressful episode. The degree of stress for mothers with cerebral palsied children was greater when the child was younger. Conclusion: Mothers of cerebral palsied children had higher stress than that of mothers with normal children and felt more stress when their child was younger. Therefore, these results suggest that health-based policies should be developed to improve the mental health of mothers with cerebral palsied children.

가정방문을 위한 물리치료사의 가정간호사의 팀웍에 관한 연구

이혜영,김진상,최진호,Lee Hea-Young,Kim Jin-Sang,Choi Jin-Ho 대한물리치료학회 2000 대한물리치료학회지 Vol.12 No.1

The purpose of this study was analyse the necessity for the Scam work at home care service. The subjects of the study were 14 home m nurses who work in community and in physical ana 16 physical therapist. The data were collected from Sep. 1 to Dec. 31, 1999. The result were analysed statistically by $X^2$, t-test. The main results of this study were as follows : 1. There were statistical significances of the general characteristics between the physical therapists and the home care nurses, 2. There were statistical significances of currencies of the home us service which the subjects experienced. 3. There were no statistical significances of the necessity of team work and the participation in home care service. 4. Regarding the effects of expectation which consisted 9 items, there were statistical significances in 8 items.

Structural and Electronic Properties of an Alkali Fulleride, $Rb_1C_{60}$

이혜영,정동운,Lee, Hye Yeong,Jeong, Dong Un Korean Chemical Society 1996 Bulletin of the Korean Chemical Society Vol.17 No.1

Structural and electronic properties of an alkali metal fulleride, Rb1C60, was studied. In spite of the chain structure with shortdistance between balls along the crystallographic a-direction, the electronic structure calculation study with the X-ray defined crystal structure shows that Rb1C60 is a three-dimensional metal at room temperature. This result is different from the magnetic experiments in which the compound was found to behave as a quasi-one-dimensional metal. Partial Fermi surface nesting is supposed to be the reason for the metal-insulator transition found in Rb1C60 at ∼50 K.

한미 FTA에서 쌀 시장만 지키면 농업개방 겁낼 필요 업다?

이혜영,Lee, Hye-Yeong 한국낙농육우협회 2006 낙농·육우 Vol.26 No.10