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채선영 ( Seon Yeong Chae ),전소영 ( So Young Chun ),박민 ( Min Park ),장유진 ( Yu Jin Jang ),김진래 ( Jin Rae Kim ),오세행 ( Se Heang Oh ),이진호 ( Jin Ho Lee ),송필현 ( Phil Hyun Song ),권태균 ( Tae Gyun Kwon ),김태환 ( Tae Hwan 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.1s
The innate extracellular matrix (ECM) scaffolds can be a promising scaffold for regeneration of complex organ such as heart, liver and kidney. They possess intact 3-dimentional architecture and biochemical components that allow to access to the organ`s capillary network. In this study we have developed a porcine renal ECM scaffold and analyzed its physical and biochemical characteristics including biocompatibility for human kidney regeneration. A segmented porcine kidney cortex was obtained and treated with 1% (v/v) Triton X-100 (Triton) or sodium dodecyl sulfate (SDS) in a shaking chamber, and rinsed with distilled water. After confirmation of decellularization with H&E stain, the matrix was lyophilized and sterilized. Scanning Electron Microscope (SEM) analysis showed that both scaffolds were preserved with proper architecture including porosity for cell adhesion and composition of the renal ECM. The water uptake ability of the Triton treated scaffold was higher than that of SDS treated one. The maximum compressive strength of Triton was lower than SDS treated scaffolds and that correlates with the results of porosity and water uptake analysis. In ATR-IR analysis, both scaffolds showed a peak at 3445-3446 cm-1 and that indicates the presence of amide II (-NH). Triton treated scaffold demonstrated that there are richer contents of ECM proteins and growth factors compared to SDS treated one. When scaffolds were seeded with primary human kidney cells, Triton treated scaffold showed 2.66 times higher number of adherent cells than SDS treated one at 24 hrs postseeding. On a CCK-8 analysis, the Triton treated scaffold showed significantly higher cell viability and proliferation rate than that of SDS treated one. Both scaffolds had no tumorigenecity for 8 weeks in vivo analysis. In conclusion, we successfully developed porcine renal ECM scaffold and confirmed that there is a great potential of porcine renal ECM scaffold to be used as human kidney regeneration. We also verified that 1% Triton X-100 is more suitable decellularizing agent than SDS regarding structural, biochemical integrity and biocompatibitilty of the scaffold. To support our findings and human application of practical regeneration, we are planning to perform in vivo experiment for kidney regeneration near future.
곰솔 해안방재림의 형상비를 고려한 밀도 관리의 필요성과 방안
김석우 ( Suk Woo Kim ),전근우 ( Kun Woo Chun ),박기형 ( Ki Hyung Park ),임영협 ( Young Hyup Lim ),윤주웅 ( Ju Ung Yun ),권세명 ( Se Myoung Kwon ),윤호중 ( Ho Joong Youn ),이진호 ( Jin Ho Lee ),( Yukiyoshi Teramoto ),( Tsugio 한국산림과학회 2015 한국산림과학회지 Vol.104 No.3
This study examined methods for stand density control by using shape ratio (tree height/DBH) and its application for effective management of Pinus thunbergii coastal disaster prevention forests. We analyzed the present conditions (height, DBH, and density) of P. thunbergii coastal disaster prevention forests at 123 study sites on Jeju Island and west, south, and east coasts of South Korea and compared them with results from previous studies. The average shape ratio for P. thunbergii showed positive correlations with stand density and was significantly higher on the west coast (66.32) than on the south (49.57) and east (48.19) coasts and Jeju Island (48.29). Stands with shape ratio higher than 70 accounted for 50% of the total study sites on the west coast, indicating a decrease in their disaster prevention function compared to that of other previous studies. The stand density in most coastal areas, except the east coast, was significantly higher than the standards recommended by the Korea Forest Service and the Forestry and Forest Products Research Institute of Japan, indicating the need for stand density control. According to the growth estimation equation for P. thunbergii in the coastal area of South Korea, density control is required for young stands less than 14 years old, which show drastic increase in the shape ratio, to conserve their disaster prevention function. Particularly, the first thinning of P. thunbergii forests should be implemented before the stand age of 8 years that a shape ratio exceeds 70. For disaster-prone young stands (≤ 20 cm DBH) of P. thunbergii, the stand density was higher in the standard of Japan considering shape ratio than in that of Korea aiming timber production. Hence, the standard guidelines employed in Japan, which assign higher importance to disaster prevention function based on field surveys, can be applied effectively for controlling the stand density of P. thunbergii coastal forests in South Korea, to improve their disaster prevention function.