수종(數種)의 약용식물(藥用植物)에서 분리(分離)한 Carlavirus에 대하여

이준탁,토거양이,Lee, Joon-Tak,Doi, Yoji 한국현미경학회 1987 Applied microscopy Vol.17 No.1

Carlaviruses were isolated from naturally infected medicinal plants, and identified by means of test plants and electron microscopy. Mottle symptoms were shown on leaves of Panax ginseng, Aralia cordata, Xanthium strumarium, Taraxacum officinale, Aconitum carmichaeli, and Bupleurum longiradiatum var. breviradiatum. Ring spot on leaves of Abutilon avicennae and ring mosaic or vein clearing on leaves of Sambucus sieboldiana were also shown. These viruses had rather narrow host ranges by mechanical inoculation. The virus particles were scattered or aggregated in cytoplasm of infected host plant leaves. The carlaviruses for which the name panax virus S (PaVS), aralia virus S (ArVS), xanthium mottle virus (XaVS), taraxacum virus S (TaVS), aconite mottle virus (AcMV), bupleurum virus S (BuVS) and abutilon ring spot virus (AbRSV) were proposed, had flexuous particles with width 13 nm and length $620{\sim}720nm$. A reported elder ring mosaic virus was isolated from leaves of Sambucus sieboldiana with ring mosaic or vein-clear symptoms.

답리작 상치 시설재배지에서의 균핵병 발생생태에 관한 연구

신동범,이준탁,Shin Dong Bum,Lee Joon Tak 한국식물병리학회 1987 Plant Pathology Journal Vol.3 No.4

답후작으로 시설재배되는 상치에 발생한 균핵병의 발생생태에 대하여 조사연구한 결과, 상치 균핵병은 전생육기간을 통하여 발생하였는데, 특히 유묘기에 피해가 심하였다. 후병지$(30\times30\times5cm)$내의 균핵수는 유묘기에 22.0개, 수확기에는 5.3개가 검출되었다. 이상치 균핵병 (S. sclerotiorum)의 균사생장적온은 $25^{\circ}C$였고, $15^{\circ}C$이하의 온도에서는 $20^{\circ}C$이상의 온도에서보다 균핵의 형성수는 적었으나 큰 균핵을 형성하였고 건물중도 높았다. 토양깊이 및 시기별로 균핵을 매몰하였을 때 3월, 4월 9월의 매몰구에서는 토양깊이 3cm구까지 자양반을 형성하였으나, 6월, 12월의 매몰구에서는 90일간의 조사기간내에는 자 반을 형성하지 않았다. 그리고 중력수만 제거한 토양과 담수토양내에서 균핵을 $25^{\circ}C$와 $30^{\circ}C$로 5주간 처리하였을 때 $100\%$의 균핵이 사멸하였다. 고압멸균된 상치 식물체절편과 함께 균핵을 상치에 접종하였을 때 균핵의 균사형성에 의한 감염이 일어났으며, 포장주위식물에 대한 병원성 조사에서는 조사식물 9과 31종중 화본료 식물을 제외한 8과 25종의 식물에 병원성을 나타냈다. 그러므로 답리작으로 시설재배되는 상치에 발생한 균핵병의 전염원은 포장주위의 기주식물에 형성된 균핵이라고 생각된다. Incidence of lettuce drop was observed throughout the growing season in the vinylhouse at the southern part of Korea, Kimhai. Occurrence of this disease was especially severe at the seedling stage. Number of sclerotia in surface soil $(30\times30\times5cm)$ was 22.0 at the seedling stage, and 5.3 at harvest in the infected area. Temperature for mycelial growth ranged from 5 to $30^{\circ}C$ with optimum temperature at $25^{\circ}C$. Sclerotia were formed fewer at low temperature, but their size was larger resulting in heavier dry weight than that at high temperature. The apothecia were formed from the sclerotia that were buried in March, April and September upto 3cm soil depth, but formed from those buried only 1 em soil depth in October. Sclerotia buried in June and December did not form apothecia regardless of soil depth by 90 days. The sclerotia buried in the 5cm of soil depth did not form apothecia. Sclerotia that were embedded in wet or flooded soil at $25^{\circ}C$ and $30^{\circ}C$ for 5 weeks lost their viability. Infection of lettuce was possible with mycelia originated from sclerotia on autoclaved lettuce plant fragments. The fungus was pathogenic on 25 plant species in 8 families in artificial inoculation tests. Lettuce seedlings appeared to be infected by airborne ascospore originated from sclerotia on crops and weeds around paddy fields, because sclerotia existing in soil might perish under long flood conditions during rice cultivation.

관상식물(觀賞植物)의 진균병(眞菌病) 발생조사(發生調査) (II)

김지영,이준탁,Kim, Ji Young,Lee, Joon Tak 경북대학교 농업과학기술연구소 1985 慶北大農學誌 Vol.3 No.-

대구(大邱)를 중심(中心)으로 한 5개(個) 지역(地域)에 재식(裁植)되어 있는 23과(科) 28속(屬) 32종(種)의 관상식물(觀賞植物)에 대(對)하여 진균(眞菌)에 이병(罹病)된 식물(植物)을 조사(調査)한 결과(結果) Uncinula 속균(屬菌) 등(等) 21속(屬)의 병원진균(病原眞菌)에 의(依)하여 26속(屬) 30(種)의 식물(植物)에서 45종(種)의 병(病)이 발생(發生)하고 있었다. 이들 중(中) 우리나라에서 이제까지 보고(報告)되지 않은 미기록병해(未記錄病害)는 Uncinula속균(屬菌), Phyllosticta속균(屬菌), Discosia속균(屬菌), Macrophoma속균(屬菌), Colletotrichum속균(屬菌), Pestalotia속균(屬菌), Phomopsis속균(屬菌), Gloeosporium속균(屬菌), Alternaria속균(屬菌), Fusicoccum속균(屬菌) 및 Sphaceloma속균(屬菌) 등(等)에 의(依)하여 15속(屬) 16종(種)의 식물(植物)에 22종(種)의 병(病)이 발생(發生)하고 있었다. The 32 species of the shade trees and ornamental plants belong to 23 families which seemed to be infected by fungal pathogens were collected at 5 localities in Korea. The diseases were diagnosed with their symptoms, the characteristics of the pathogens and the pathogenicity on host plants. The results were summarized as follows ; The 45 diseases by 21 genera of fungal pathogens were investigated on 30 species of the shade trees and ornamental plants. Among them 22 diseases by 13 genera of fungal pathogens were turned out to be new diseases which have not hitherto been described on the host plants of 16 species in Korea.

야생 산마늘의 휴면 생리 및 연화 재배

최상태(Sang Tai Choi),이준탁(Joon Tak Lee),박우철(Woo Churl Park) 한국응용생명화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.6

This experiment was carried out to find out the dormancy physiology, method of softening culture and evaluation of nutritional value of wild garlic, Ulrung-native Album victorialis var. platyphyllum. In March, a new bulbs, the shoot and bulbs began to develop until the bulbs showed their complete dormant states in late August. The bulbs renewed to another one in every years. When shoots germinated about 1∼2 ㎝ from mother bulbs, the soft tissues in the mother bublbs was degenerated and finally remained as only fiberous tissues unlike the other bulbaceous plants. There was a high inhibiting activities like ABA in the bulbs. This is believed that this inhibiting substance like ABA in the bulbs is related to the dormancy of wild garlic. Although the immatured bulbs, harvested at May and June, was treated with chilling for 90 days, it didn`t germinate their shooting, but the matured bulbs, harvested at July and August, could germinate their shooting over 1 ㎝ in 75 and 60 days chilling treatment, respectively. The shoot elongation was promoted by the longer chilling periods, the later harvesting day and the dark condition. The crude fiber content of leaf and stem increased at more expanded leaf and higher light intensity condition. Since the shoots, grown from germinating to leaf expanding time, had a good quality for food stuff and had less crude fibers, we supposed this period is to be most appropriate for harvesting time.

야생 산마늘의 생육 환경과 영양 평가

최상태(Sang Tai Choi),이준탁(Joon Tak Lee),박우철(Woo Churl Park) 한국응용생명화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.6

In Ulrung floral community the growing environments and nutritive values of wild garlic was investigated. The wild garlic, grown for 3∼4 years in community area, were able to develope bulbs. The bulbs with 2 leaves had the hightest ratio of propotion as 75.1% of share among the distribution of bulbs with completely developed leaves and 1 leaf-bulbs and 3 leaves-bulbs had 19.6% and 5.3%, respectively. Also the 2 or 3 leaves-bulbs indicated the better growth states in the part of shoot and bulbs than 1 leaf-bulbs, and there was a significant differences in each growth between 2 leaves-and 3 leaves-bulbs. On the wild garlics we cannot find out insect injuries. However, there was deceases with symptoms of leaf-spots and lesion from May to June. At first it made a small spots on the leaves, followed by senescence at arrounding area of spots, and then it expanded to whole leaf. This pathogenic bacterium was supposed to be a member of Aristastoma. The contents of crude protein, fat, and carbohydrate in each portion of wild garlics decreased in the order of shoot, expanded leaves and bulbs. On the contrary, crude fibers increased in the same order and so it is not suitable for human consumption nutritional value. However, because the shoots of wild garlics have more than the other Album sp., the shoots will be a good quality food stuff.

Backstepping 기법을 이용한 서보시스템의 제어

윤기영(Ki-Young Yun),지석준(Suk-Jun Ji),최우진(Woo-Jin Choi),이준탁(Joon-Tak Lee) 대한전기학회 2003 대한전기학회 학술대회 논문집 Vol.2003 No.7

藥用植物眞菌病에 關하여(Ⅰ)

李準琸,金文鎬,朴京順 慶北大學校 1983 論文集 Vol.36 No.-

The 27 species of the medicinal plants belong to 20 families which seemed to be infected by fungal pathogenes were collected at 8 localities in Korea during 1981 and 1982, and the diseases were diagnosed on the basis of their symptoms and the characteristics of the pathogenes. The results were summarized as follows; The anthracnose on 10 species, powdery mildew on 6 species, rust on 3 species, white rust by Albugo sp. on 2 species, respectively, and leaf spot by Septoria sp. and Phyllosticta sp., smut by Ustilago sp., grey mold by Cercospora sp., pale spot by Ramularia sp., ring spot by Pestalotia sp., and pod blight cladosporium sp. were found on each one species of the plants. Among them 11 diseases were turned out to be new diseases which has not hitherto been described on those hosts in Korea.

藥用植物 Virus의 調査

李準琸,土居養二,與良 淸 韓國自然史博物學會 1981 生物과 自然 Vol.11 No.2

斑紋과 mosaic 등의 病徵을 나타내는 樂用植物을 1979年과 1980年에 日本의 各地에서 採集하여 汁液 接種과 電子顯微鏡觀察에 依해서 調査한 結果 34科 58種이 植物에서 各種의 virus를 檢出하였다. 長形 virus로서 carlavirus가 7種, potyvirus는 20種의 植物에서 檢出되었고 球形 virus에 있어서는 CMV가 21種, comovirus인 BBWV가 8種, nepovirus가 2種의 植物에서 檢出되었으며 gemini-virus가 2種의 植物에서 檢出되었다. 그리고 未同定의 長形 virus 粒子가 2種, 球形 virus 粒子가 7種의 植物에서 檢出되었으며 이들중 23種의 植物 virus 病은 未記錄인 新病害라 생각된다. The medicinal plants of 73 species in 43 families showing mosaic and mottling symptoms were collected from 1979 to 1980 at 13 areas in Japan. Various viruses were detected from 58 species in 34 families among them by sap inoculation tests and electron microscopy. It was observed that seven species of medicinal plants were infected by carla-viruses, 20 by potyviruses, 21 by cucumoviruses, eight by comoviruses, two by nepoviruses, and two by geminiviruses. New virus diseases that have not been reported in Japan were found from 23 species of medicinal plants.

사과 탄저병균의 월동에 관한 연구

이준탁,김문호 慶北大學校 生産技術硏究所 1970 生産技術 Vol.4 No.-

This study put emphasis on investigating the over-witering portion of the pathogen as one of the efficient control systemes of apple bitter rot, because of the fact that this bitter rot spreades rapidly on fruits and causes great losses of apple production as this disease broken out. The obtained results were as follows. 1). The spores of Glomerella cingulata were flowing out continually from diseased fruits like the over wintering diseased fruit, until early August that the bitter rot was occurring on the fruit. 2). Bitter rot of apple occured on the fruit of the branch was inoculated with the pathogenes, and the pathogen was isolated from branches with diseased fruits. 3). Living period of spores from each plot, namely, on the leaves and the fruit were 13 days except in water. Therefore, these showed that the spore can not tide over the winter. 4). Bitter rot fungi were isolated from experimented Drosophila rared on the diseased fruit by 60.7%. As the above results, authors guess that Drosophila transfers the fungi from diseased fruit and diseased branches to the healthy fruits. Thus bitter rot occurs.

藥用植物의 遺傳資源蒐集과 利用에 對한 調査

李準琸 慶北大學校農業科學技術硏究所 1990 慶北大農學誌 Vol.8 No.-

1987年부터 1989年까지 慶北地域을 中心으로 慶南과 忠北의 一部地域에서 調査한 藥用資源植物은 118科 331屬 456種이었고, 이들중 Compositae, Liliaceae, Leguminosae, Labiatae, Rosaecae, Polygonaceae, Umbelliferae, Gramineae, Solanaceae, Cucurbitaceae, Ranunculaceae, Cruciferae, Rutaceae, Araliaceae, Araceae, Moraceae, Scrophulariaceae, Caryopyhllaceae, and Convolvulaceae 등 19科에 속하는 식물이 249種으로 調査된 植物의 半數以上을 차지하고 있었고 나머지 207種은 99科의 植物로 分類되었다. 調査植物의 거의 大部分이 多年生이었으며, 開化期는 대부분 6-8月이었다. 栽培圃場과 展示圃場, 庭園, 公園 等地에 栽植되어있는 78科 213種의 植物중에서 藥用資源植物의 主用途는 觀賞用이 93種, 藥用 50種, 菜蔬類 27種, 食品用 14種, 果菜類 5種, 果樹類 13種, 油料用 5種, 茶用 6種, 綠音樹 및 街路樹用이 9種, 纖維用 등 기타의 用途가 14種이었다. 調査植物의 藥用部位는 地下部가 가장 많았고 그 다음은 莖葉, 全草, 花器, 種子 및 果實의 順이었다. 藥用效能은 利尿, 胃腸病, 强壯, 鎭痛, 解熱, 鎭咳 止血, 消炎作用의 植物이 대부분이었으며, 抗癌效果의 植物도 9種 調査되었고, 漢藥劑로 많이 利用되고 있는 植物은 87科 191種이었다. In order to collect the plant genetic resources in Korea, the medicinal plants were surveyed from 1987 to 1989 in Kyungbuk province and at the several areas in Kyungnam and Choongbuk, province. The medicinal plants of 456 species in 118 families were investigated in these regions. Among them, 249 species were belonged to 19 families, Compositae, Liliaceae, Leguminosae, Polygonaceae, Labiatae, Rosaceae, Umbelliferae, Gramineae, Solanaceae, Cucurbitaceae, Ranunculaceae, Cruciferae, Rutaceae, Araliaceae, Araceae, Moraceae, Scrophulariaceae, Caryopyhllaceae, and Convolvulaceae, and others were belonged to 99 families. They have been used mostly for urination, gastroenteric disoder, robustness, antiphlogistic, anodyne, cough medicine, fever remedy, hemostatic, and 9 species of them have been used for anticancer medicine. The medicinal plant resources which were planted in the fields, gardens, parks, roadsides, and glass house, were 93 species for ornamental, 50 for medicine, 27 for vegetable, 14 for food, 5 for vegetable fruit, 13 for fruit, 5 for oil, 6 for tea, 9 for shade, and 14 for other purposes. Most of investigated plants were perennial, and flowered from June to August. The root parts were most frequently used as medicine, and other parts were used in the order of leaf and stem, whole plants, flower, seed, and fruit. Among the 456 species, 191 species in 87 families were investigated to be important medicinal plants.