저장장소 및 포장재료가 섬바디 종자의 발아율에 미치는 영향

한흥전,양종성,이종렬 ( Heung Jeon Han,Jong Seong Yang,Jong Yeol Lee ) 한국축산학회 1981 한국축산학회지 Vol.23 No.2

This experiment was conducted to investigate the storage method of Seombadi seed(Dystaenia takesimana Nakai) in 1978, and 1979. The tested seeds were stored for 26 and 16 months at -10℃and room temperature in paper, polyethylene and cotton bags, respectively. The results are summarized as follow : 1. regardless of treatments, sound seeds of Seombadi required in general about 40 days for germination. 2. Until 26 months after harvest the germination rate of seed stored at -10℃ was 41.7 to 55.5 percentage but seed stored at room temperature was nearly zero percentage in 12 months after harvest. 3. In case of storage at low temperature (-10℃), the germination rate of seed produced in both years was similar regardless of packing materials. 4. In case of storage at room temperature, the germination rate of seed produced in 1978 was different in accordance with packing materials. After storage for 12 months at room temperature, the germination rate of seed packed with paper or cotton bags was 9.7 or 8.0 percentage, but the seed packed with polyethylene was 52.3 percentage at 16 months after harvest. It was concluded that, polyethylene bag as packing material for seed storage at room temperature was effective.

연구논문 : 내륙습지 기능의 우선순위 결정

이승준 ( Seung Jun Lee ),최현아 ( Hyun Ah Cho ),이우균 ( Woo Kyun Lee ),이종렬 ( Jong Yeol Lee ),전성우 ( Seong Woo Jeon ),김준순 ( Joon Soon Kim ) 한국환경영향평가학회 2014 환경영향평가 Vol.23 No.3

In this study, the functional priority of wetlands was determined through analysis of previous research. To determine relative importance, three processes were performed. First, quantitative values from the case studies were normalized. Second, non-quantitative values were prioritized based on standard criteria. Third, equal weight was applied as long as there was no special consideration regarding a particular value`s disproportionate priority in the research. Finally, results were grouped into large, medium, and small classes. In this study, the functions of the medium class were found to be the most significant, in the following order of priority: water supply and ground water recharge; culture and recreation; biodiversity; product; water quality control; flood control; erosion control; moderation of climate change; and provision of biochemical matter. To verify these results, we compared our findings with those of an assessment that used the Rapid Assessment Method (RAM) on the same type of study area. Whereas this comparison indicated some correlations by the culture, water storage, and genetic sustainability functions, it suggested a lack of such relationship by the water purification and flood prevention functions.

홍색 비황 광합성 세균 Rhodopseudomonas sp. KH4의 Extracellular polysaccharide의 중금속 흡착

정정화,서필수,공성호,이종렬,이상섭,Jeong, Jeong-Hwa,Seo, Pil-Soo,Kong, Sung-Ho,Lee, Jong-Yeol,Lee, Sang-Seob 한국미생물학회 2006 미생물학회지 Vol.42 No.4

본 연구에서는 경기도 안양에 있는 하천에서 분리한 홍색 비황 광합성 세균Rhodopseudomonas sp. KH4에서 생성된 extracellular polysaccharide (EPS)의 중금속 흡착특성에 대해 연구하였다. 100 mg/L의 Cd과 Cu의 최적 흡착조건(온도$40^{\circ}C$ Cd; pH 8, Cu; pH 5)에서 1.0 g/L의 EPS를 첨가하였을 때, Cd의 경우 30분 동안 842 mg/L, Cu의 경우 10분 동안 70.0 mg/L가 흡착되었다. 또한 Cd과 Cu가 각각 100 mg/L씩 공존되어 있는 경우, pH 5, $25^{\circ}C$에서 EPS 1.0 g/L 첨가하였을 때 Cd과 Cu가 각각 16.8 mg/L, 48.7 mg/L가 흡착되었다. 또한 Langmuir 등은 흡착식으로 부터 EPS 1.0 g/L, pH 5, $25^{\circ}C$에서 Cd과 Cu의 흡착량은 각각 76.9 mg/L, 67.1 mg/L로 실제 경험치와 큰 차이를 보이지 않았다. 에탄올 침전법으로 정제된 KH4 strain으로부터 생성된 EPS는 gas-liquid chromatography (GLC) 분석에 의한 중성당 분석 결과, arabinose, glucose, mannose가 각각 2.4%, 7.1%, 90.5%로 mannose가 대부분을 구성하였다. In the present study, we examined biosorption characteristics of heavy metals onto the extracellular polysaccharide (EPS) produced by the purple nonsulfur photosynthetic bacteria Rhodopseudomonas sp. KH4, which was isolated from a stream in Anyang, Kyonggi-Do. When Cd (100 mg/L) and Cu (100 mg/L) were added to EPS (1.0 g/L) in the optimal condition (Cd; pH 8, Cu; pH 5, $40^{\circ}C$), 84.2 mg/L of Cd and 70.0 mg/L of Cu were adsorbed within 30 min and 10 min, respectively. When 100 mg/L of Cd and Cu were present as mixture, 16.8 mg/L of Cd and 48.7 mg/L of Cu were adsorbed at $25^{\circ}C$, pH 5. The maximum adsorption capacity determined by fitting Langmuir isotherms model was suitable for describing the biosorption of Cd (76.9 mg/g) and Cu (67.1 mg/g) by EPS. The neutral monosaccharide in the EPS determined by GC consisted of arabinose (2.4%), glucose (7.1%) and mannose (90.5%).

겉뿌림 초지에 대한 삼요소시비수준이 초지생산성 및 식생에 미치는 영향

진신흠,고서봉,윤익석,이종렬,김문철 ( Sin Heum Jin,Seo Bong Ko,Ik Suk Yoon,Jong Yeol Lee,Moon Chul Kim ) 한국축산학회 1980 한국축산학회지 Vol.22 No.3

Orchardgrass (Dactylis glomerata C.), tall fescue (Festuca arundinacea L.), perennial ryegrass (Lolium perenne L.), Kentucky bluegrass (poa pratensis L.), and ladino clover ( Trifolium repens L.) were sown on a native grassl and dominated by Imperata cylindrica by the oversown method. When the different levels of nitrogen, phosphorus and potassium were applied, the following results were obtained : 1. The dry matter yields of whole pasture were increased by increment of nitrogen fertilizer. Of the newly sown pasture plants, the botanical composition of grasses was increased, but that of clover was decreased. 2. The botanical composition, however, was not influenced by the phosphorus and potassium level. The rate of the newly sown pasture plants in the second year was 20-63% and the ratio of the grasses and legume was 60 : 40. 3. There was statistically marked significance for the dry matter yield by the increment of nitrogen levels; 122.4㎏ (0 ㎏ N), 182. 6㎏ (O ㎏ N) and 230. 9㎏ (20㎏) per 10 a were shown in the first year while 111. 3㎏ (0 ㎏ N), 135. 2㎏ (10 ㎏ N) and 182. 8㎏ (20 ㎏ N) per l0a in the second year. 4. There was no difference among dry matter yields obtained from various phosphorus and potassium levels in the first year, whereas some increased yield was observed in the second year.

연구보문 : 자연과학 ; 자포니카 벼의 종자 저장단백질 유전자 구조 및 발현분석 연구 현황

윤웅한 ( Ung Han Yoon ),이정화 ( Jeong Hwa Lee ),이강섭 ( Gang Seob Lee ),김영미 ( Young Mi Kim ),한장호 ( Jang Ho Hahn ),지현소 ( Hyeon So Ji ),윤성원 ( Sung Won Yoon ),이종렬 ( Jong Yeol Lee ),김태호 ( Tae Ho Kim ) 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.3

Based on the outcome of the rice genome sequencing, a lot of researches for identification of genes underlying useful traits and their utilization are being carried out recently. Especially, rice seed storage proteins are used as a main nutrition source for human while they are used as the nitrogen source for plant growth during seed germination. Rice seed storage proteins are divided by solubility in solvent into weak acid and alkali soluble glutelin, alcohol soluble prolamin, and salt soluble globulin. The rice genome contains 11 glutelin genes and 33 prolamin genes. In the case of prolamin genes, 15 genes are located in 15 Mb region on the chromosome 5. Clustering of seed storage protein genes on the same chromosomal region indicates that they are evolved from the same chromosome or they share similar gene expression regulatory region. Only single globulin gene existed on the chromosome 5. As a result of tissue- specific expression pattern analysis of seed storage protein genes using microarray, glutelin and most of prolamin genes were expressed only in seed endosperm, while a few prolamin and the globulin genes are expressed in seed endosperm and germinating seed. As a result of isolating promoter regions of the seed storage protein genes and analyzing their expression pattern in seed, glutelin type C1 promoter showed expression in whole seed, and glutelin type B5 and α-globulin promoter showed strong expression. This paper reviewed on the structure and expression profile of rice seed storage protein genes, and will provide information on the recent research status to the researchers who are studying on the improvement of seed traits and production of substances.

위성영상과 공간자료를 이용한 북한 지역의 재조림 CDM 대상지 선정 및 적지분석 방안

유성진(Yoo, Seong Jin),이우균(Lee, Woo Kyun),이승호(Lee, Seung Ho),김은숙(Kim, Eun Sook),이종렬(Lee, Jong Yeol) 대한공간정보학회 2011 대한공간정보학회지 Vol.19 No.3

본 연구의 목적은 북한 지역을 대상으로 위성영상과 공간자료를 이용하여 재조림 CDM의 정의에 적합한 대상지 선정 방안을 제시하는데 있다. 재조림 CDM이 가능한 대상지는 1990년 이전부터 현재까지 산림이 아닌 지역으로 규정되어 있다. 연구대상지역은 북한 함흥 지역이며, 1988년 9월 27일 취득된 Landsat TM 영상과 2007년 9월 24일 취득된 SPOT Pan-sharpened 영상의 두시점 영상을 이용하였다. 두시점의 영상에 대해서 각각 영상분류를 실시하여 산림황폐지(무립목지, 개간산지, 산간나지)를 구분하였다. 그리고 두 영상분류 결과를 이용한 변화탐지 분석을 수행하여 잠재적인 재조림 CDM 대상지를 도출하였다. 영상분류 결과, 19년간 신림의 1,214 ㏊가 개간산지, 농경지 또는 산간나지로 변화된 것으로 분석되었다. 분석 결과, 2,245 ㏊가 재조림 CDM의 정의에 부합하는 것으로 나타났으며, 전체 잠재적 재조림 CDM 대상지 중 79.2%가 개간산지로 나타났다. 분석된 잠재적 재조림 CDM 대상지에 대하여 지형 및 접근성 분석을 통해 적합성 지수를 산출하였고 대상지를 적합성에 따라 등급화하여 사업우선 순위의 선정 등에 활용할 수 있도록 하였다. The objective of this study was to select appropriate sites for reforestation CDM using satellite image and spatial data in North Korea. A suitable site for reforestation CDM can be defined as non-forest area since 1990. To detect land cover change between two different years and to delineate potential reforestation CDM site(unstocked forest, converted crop land, denuded bare land), Landsat TM satellite image which was scanned in 1988 and SPOT Pan-sharpened image which was scanned in 2007 were used. As a result of classification, 1,214 ㏊ of forest area in 1989 had been converted to other land cover types in 2007. and 2,245 ㏊ of the total study area was detected to be suitable for reforestation CDM. 79.2% of total potential CDM sites was converted crop land. Through topography and accessibility analysis for potential reforestation CDM sites detected by satellite image, suitability index was calculated and the potential reforestation area was reclassified into suitability grades.

밀 형질전환과 이를 활용한 최신 연구동향

심재령,김세원,이수빈,김범기,이샛별,이종렬,Sim, Jae-Ryeong,Kim, Sewon,Lee, Su-Bin,Kim, Beom-Gi,Lee, Saet Buyl,Lee, Jong-Yeol 한국작물학회 2020 한국작물학회지 Vol.65 No.4

Wheat is one of the world's top three crops and is an important staple crop, accounting for 20% of the nutrient calories consumed by the world's population. However, due to its complex heterogeneous hexaploid chromosomes and vast genome of approximately 16 Gb, compared to those of other crops, molecular biology and biotechnology studies on wheat are lacking. In recent years, wheat genome analysis has been performed using the latest next-generation sequencing technology so that useful genes can be easily obtained, and wheat biotechnology research is accelerating in various fields. In this review, wheat transformation, an indispensable technique for developing new functional biotech wheat by revealing the function of wheat genes, is described in detail. In addition, the latest research results for overcoming plant diseases, abiotic stresses, and wheat-related diseases that are difficult to solve by classical breeding through wheat transformation and biotechnology are described.

비스코스 레이온 직물의 방축가공

김성동(Sung Dong Kim),이인열(In Yeol Lee),이종렬(Jong Lyel Lee),김민정(Min Jung Kim) 한국염색가공학회 1998 韓國染色加工學會誌 Vol.10 No.6

다양한 비생물적 스트레스에 의해 유도되는 RsMYB1 프로모터의 특성분석

김다혜(Da-Hye Kim),양주희(Ju-Hee Yang),이주희(JuHee Rhee),이종렬(Jong-Yeol Lee),임선형(Sun-Hyung Lim) 한국육종학회 2021 한국육종학회지 Vol.53 No.4

Plants grown under stress conditions generate excessive reactive oxygen species resulting in cell death. Therefore, plants activatethe protection mechanism via antioxidant accumulation. Anthocyanins are flavonoid-derived secondary metabolites with high antioxidantproperties. In this study, we analyzed and characterized the promoter region of RsMYB1, a positive regulator of anthocyanin biosynthesis. The RsMYB1 promoter was designed with four different fragment lengths (MP1, -1034; MP2, -830; MP3, -633; and MP4, -430 bp), andthen each RsMYB1 promoter region was fused into a GUS gene for Arabidopsis transformation. The expression patterns of the RsMYB1 promoterconstructs were analyzed at different developmental stages and under various abiotic stresses. The GUS expression pattern steadily increasedwith plant growth, and coincided with enzyme activity and a histochemical GUS assay. In response to drought, salt, sucrose, and low temperature,the GUS transcript level was highly expressed in MP4 in parallel with GUS enzyme activity. These assays indicated that the proximal region(-430 to -1 bp) of RsMYB1 was the core sequence that was induced by salt and low temperature. The expression level of RsMYB1 in theleaves of radish was highly activated and was consistent with the anthocyanin content under salt and low temperature conditions. These resultssuggest that induction of the RsMYB1 gene can activate the biosynthesis of anthocyanins, which are expected to help plants adapt to stressconditions due to their antioxidant activity.

식물 발달과 스트레스 반응에 관여하는 ERF 유전자군의 다양한 역할과 조절기작

박상규(Sangkyu Park),김다혜(Da-Hye Kim),박성옥(Sung-Ok Park),이종렬(Jong-Yeol Lee),임선형(Sun-Hyung Lim) 한국육종학회 2017 한국육종학회지 Vol.49 No.4

APETALA2/ethylene response factor (AP2/ERF) transcription factors are involved in biological and abiotic stress response, plant development, and growth. AP2/ERF genes are classified into five families (AP2, DREB, ERF, RAV, and soloist), and most genes belong to DREB and ERF families. So far, genomic analysis of DREB and ERF family genes of various plant species has been performed, and classifications based on the homology of AP2/ERF-specific DNA binding domain, arrangement of exons and introns, and similarity of group-specific conserved motifs have been conducted. These classifications provide plausible information for the prediction of AP2/ERF gene function. In this paper, an overview of the classification, structure, evolution, and function of AP2/ERF genes is described, and the functional properties and regulatory mechanisms of ERF family genes that have been identified are summarized by group according to the functional classification of Arabidopsis ERF family genes. This shows that group-specific conserved motifs of Arabidopsis ERF family genes are closely linked with group-specific functions and regulatory mechanisms, indicating that the effective functional prediction of ERF family genes through such a classification scheme can be usefully applied to the trait improvements of various plants.