부자 추출물이 LPS로 유도된 C57BL6 마우스의 패혈증 연관 급성 폐 손상에 미치는 영향

이인승,부민아,심재욱,백승호,박진봉,In-Seung Lee,Mina Boo,Jae Ouk Sim,Seung-Ho Baek,Jinbong Park 대한융합한의학회 2022 대한융합한의학회지 Vol.4 No.1

Objectives: TSepsis and subsequent acute lung injury (ALI) is a critical state of health caused by infection or endotoxins. This study was conducted to evaluate the effect of Water Extract of Aconiti Lateralis Preparata Radix (AR) on lipopolysaccharide (LPS)-induced sepsis in C57BL/6 mice. Methods: Male C57BL/6 mice were intraperitoneally injected with LPS to induce sepsis and ALI. AR was orally fed twice at 30 min and 180 min after LPS injection. At 24 h post injection, mice were sacrificed, bronchoalveolar lavage fluid (BALF) and blood was collected, and lung tissue was harvested. Hematoxylin and eosin staining was performed in lung tissues, wet/dry ratio of the lung tissue was measured, and the serum cytokine and chemokine levels were analyzed. Results: AR revoked the LPS-induced pathological changes in lung tissues, such as abnormal histological structures, immune cell infiltration and lung edema. Also, AR suppressed the neutrophil infiltration into the lung which was greatly increased by LPS injection based on the cell content of collected BALF. Serum cytokines and chemokines were measured, and AR reversed the LPS-induced increase of cytokines such as interleukin 1 beta, interleukin 6, tumor necrosis factor alpha and chemokines including C-X-C motif chemokine ligand 1 and 2. Conclusion: TAR showed a protective effect in the pathological progress of LPS-induced ALI. Especially, AR suppressed lung edema and infiltration of neutrophils by inhibiting cytokine and chemokine expressions. Such results demonstrate the potential of AR as a therapeutic agent for sepsis and sepsis-induced ALI.

나의 신장을 팔아서라도

이인승 새가정사 1986 새가정 Vol.- No.-

백서 창상의 피복방법에 따르는 Muofibroblast의 생성 및 소실되는 과정의 실험적인 비교관찰

김한중,최희윤,조철현,이인승 大韓成形外科學會 1985 Archives of Plastic Surgery Vol.12 No.1

The question of how skin coverage affects contracting wounds has long been a significant problem in the study of wound contraction. Myofibroblasts appear to be well established as the cellular cause of wound contraction. Myofibroblasts are found in many types of contracting wounds, including granulating wounds and hypertrophic burn scars. Myofibroblasts in contracting wounds have definitive life cycles, being present when contraction is active and then disappearing when contraction has ceased. In this study, we observated comparatively quantitative changes in the surface area by planimetry and changes of myofibroblasts by electron microscope through 8 weeks according to the types of skin coverage. The purpose of this study was to evaluate the myofibroblast population in skin coveraged wounds in a rat model, which allowed frequent biopsies as well as precise identificaion of the interface of the skin coverage and the wound bed. The results of our experiments are as follows: 1. Granulating wound Contracted rapidly and had long persistence of myofibroblasts. 2. Split-thickness skin graft Contrated less and myofibroblasts were more rapidly decreased and were disappeared at 5 weeks later. 3. Full-thickness skin graft Minimum contracti aondnmyofibroblasts were very rapidly decreased and were disappeared at 4 weeks later. 4. Local flap No contraction and could not find myofibroblast. Slowly increase in size at begining. 5. Contraction was influenced by number and lasting time of myofibroblast.

Extreme Learning Machine 기법 기반 무인수상드론 경로제어 연구

이인승(Inseung Lee),이덕진(Deokjin Lee) 대한기계학회 2018 대한기계학회 춘추학술대회 Vol.2018 No.5

소아에서 정맥 주사의 혈관외 삼출에 의한 피부 결손의 임상 치험예

최희윤,이인승,김한중,조철현 大韓成形外科學會 1984 Archives of Plastic Surgery Vol.11 No.4

Peripheral intravenous therapy is usually innocuous, but skin necrosis can result when gross extravasation occur. These adverse sequelae are seen infrequently in the adult patient, but in newborns and young children, they may be catastropic. The purpose of this study in to reveal the agents and locations of skin necrosis, and to evaluate the results of therapeutic management.. During the period from March 1,1981 through February 28, 1983, the children who had taken intravenous fluid therapy in our hospital were reviewed. The results are summarized as follows. 1. There was 1,275 extravasation patients among 3,290 intravenous fluid treated children patients during a 2-year period. 2. The frequent locations of skin necrosis were scalp, dorsum of foot, and dorsum of hand in descending order, but the most frequent locations of skin necrosis to the extravasation in rate were dorsum of foot, sole, and wrist. 3. The frequent agents of skin necrosis were cloxacillin, sod. bicarbonate, and cal. gluconate in descending order, but the most frequent agents of skin necrosis to the extravasation in rate were epinephrine, cal. gluconate. sod gluconate, sod. bicarbonate. 4. Authors found no disernible differences in the healing time among the 3 different methods of treatment used.(saline, sofra-tulle, povidone-iodine)

외상에 의한 안면 기형의 재건치험례

최희윤,이인승,강원경,김한중 大韓成形外科學會 1986 Archives of Plastic Surgery Vol.13 No.2

The initial reaction of a person to any damage to his face is commonly one of acute distress, which may be followed by periods of mouring and prolonged depression. So facial deformities due to traumatic injury are carefully reconstructed with individual considerations. Successful reconstruction of facial deformities is a difficult and intriguing problem, and various surgical procedure have been proved for the repair of facial deformities depending upon their causes, locations, and associated with other lesions. This article deals with 2 cases of traumatic facial deformities; One patient had frontal bone defect, eyelid ectropion, eyebrow loss, saddle nose deformity by traffic accident, and another one had generalized hypertrophic scar on face, eyebrow loss, eyelid ectropion, upper lip deformity by burn which were required various surgical procedure. So we repaired these facial deformities with more reliable, easy accessible procedures with good results.

Proteins Derived from Cnidium officinale Makino React with Serum IgE of Allergic Patients and Stimulate ERK/NF-kB Activation in Human Mast Cell Line HMC-1 Cells

박지영,이인승,김유미,안은진,Jung Woo-Sang,장형진 한국바이오칩학회 2021 BioChip Journal Vol.15 No.2

Herbal products are the most representative alternative medicine, but most herbal drugs are taken orally and these can cause allergic reactions in the gastrointestinal tract such as food allergies. To confi rm whether herbal proteins can cause allergic reaction by binding to human serum immunoglobulin E (IgE), we selected sera from 800 randomized human sera using Allergy-Q tests and investigated the allergenic reactivity of Cnidium offi cinale (CO) Makinoe extract with positive sera in HMC-1 cells. The proteins derived from CO extract were separated by SDS-PAGE, and identifi ed these proteins using LC–MS/MS. The human sera IgE binding proteins were estimated at the four diff erent predicted proteins such as FAR1-related sequence 5-like protein, a carrier protein, a hypothetical protein, and a predicted protein. The number of positive sera reacted with CO extract was 18 in 800 donor blood (2.25%). In HMC-1 cells, a co-treatment of the positive sera containing COspecifi c IgE with CO extract induced synergy eff ects in ERK/NF-kB activation compared with a sole treatment of human sera, but negative sera did not show a signifi cant change. This results imply that herbal allergens can cause allergic reaction through the crosslinking with human serum IgE, and it may trigger hypersensitivity of body immune system.

실리콘 태양전지 응용을 위한 황 결핍 n형 MoS2 층 연구

김근주,이인승 한국반도체디스플레이기술학회 2023 반도체디스플레이기술학회지 Vol.22 No.3

We investigated the MoS2 thin film layer by thermolytic deposition and applied it to the silicon solar cells. MoS2 thin films were made by two methods of dipping and spin coating of (NH4)2MoS4 precursor solution. We implemented two types of substrates of microtextured and nano-microtextured 6-in. Si pn junction wafers. The fabricated MoS2 thin film layer was analyzed, and solar cells were fabricated by applying the standard silicon solar cell process. The MoS2 thin film layer of sulfur-deficient form was deposited on the n-type emitter layer, and electrons, which are minority carriers, were well transported at the interface and exhibited photovoltaic solar cell characteristics. The cell efficiencies were achieved at 5% for microtextured wafers and 2.56% for nano-microtextured wafers.

Identification of possibility of Glycyrrhiza uralensis as an allergen by protein analysis

안은진,김강훈,이인승,박지영,김유미,정우상,권대호,장형진 한국바이오칩학회 2018 BioChip Journal Vol.12 No.1

Various secondary metabolites derived from plants are useful for humans to preserve their health. For this reason, medical herbs have been consumed universally without deep research about the dangerousness of these substance. However, they can produce unexpected adverse reactions in humans. Among these side effects, allergic reactions involving human immunoglobulin E can occur due to antimicrobial or antibacterial substances in plants. Glycyrrhiza uralensis has been prescribed for the treatment of numerous diseases given its multiple types of pharmaceutical efficacy, including its antimicrobial and antibacterial activities. Therefore, we researched whether Glycyrrhiza uralensis, which has antimicrobial ability, can cause allergic reaction in humans. To test the hypothesis devised here, we conducted proteomic level experiments. We utilized SDS-PAGE using a protein extract of Glycyrrhiza uralensis, with Coomassie blue staining to confirm the protein pattern and western blotting to identify Human IgE binding proteins in Glycyrrhiza uralensis. Among the many candidate sera, these methods detected three positive sera. Moreover, an identified protein section with reactivity associated with Human IgE was analyzed by LC-MS/ MS. Our experimental data provide a basis for testing the possibility of Glycyrrhiza uralensis as an allergen by identifying the reactivity between human Immunoglobulin E and proteins of Glycyrrhiza uralensis.

전기추진선박 통합 하드웨어 시스템 설계 및 선측 압력에 관한 연구

이지호(Ji Ho Lee),이인승(In Seung Lee),이덕진(Deok Jin Lee) 대한전기학회 2017 정보 및 제어 심포지엄 논문집 Vol.2017 No.4