복령 ( Poria cocos ) 의 배양학적 특성에 관한 연구

홍인표,이민웅 ( In Pyo Hong,Min Wong Lee ) 한국균학회 1990 韓國菌學會誌 Vol.18 No.1

눈꽃동충하초의 생리활성에 관한 연구

허현,이민웅,홍인표,Hur, Hyeon,Lee, Min-Woong,Hong, In-Pyo 한국버섯학회 2005 한국버섯학회지 Vol.3 No.2

This study was carried out to investigate the biological activities of P. tenuipes formed on silkworm (Bombyx mori) variety. The mean content of total amino acid in the fruiting bodies of P. tenuipes was $1.03{\mu}mole/g$. The distribution rate of amino acid components decreased in the order of Arginine(12.2%) > Glycine(10.5%) > Proline(9.6) > Tyrosine(8.9%) > Serine > Leucine > Threonine. The most abundant amino acid in the fruiting bodies of the Baegokjam, Chilbojam and Hachojam infected with P. tenuipes was arginine, while Yangwonjam was Glycine. The most abundant fatty acid in P. tenuipes was Oleic acid on a dry weight basis. The unsaturated fatty acids such as Oleic acid, Linoleic acid and Linolenic acid accounted for more than 78% of the total fatty acids. 경제적 가치가 매우 높은 눈꽃동충하초를 누에 품종별로 생리활성에 관한 연구를 수행한 결과를 요약하면 다음과 같다. 1. 눈꽃동충하초의 유리당 함량은 백옥잠과 하초잠에서는 Glucose함량이 가장 높았으며, 칠보잠과 양원잠에서는 Mannitol 함량이 많았다. 2. 눈꽃동충하초에 함유된 아미노산의 총 평균 함량은 $10.3{\mu}mole/g$이며, 백옥잠이 $10.7{\mu}mole/g$로 가장 높았으며, 하초잠과 칠보잠이 $10.5{\mu}mole/g$, 양원잠이 $0.96{\mu}mole/g$로 가장 낮은 함량을 나타내었다. 16종의 구성아미노산 분포비율은 Arginine이 12.2%로 가장 높았으며, 다음으로 Glycine(10.5%) > Proline(9.6) > Tyrosine(8.9%) 순이었으며, Methionine이 2.1%로 가장 낮게 분포하였다. 3. 눈꽃동충하초의 유리 지방산 조성은 누에 품종에 관계없이 Oleic acid 함량이 가장 높았으며, 다음이 Linolenic acid, Palmitic acid, Linoleic acid 순이었다.

붉은자루동충하초의 균사생육에 관한 연구

홍인표 ( In Pyo Hong ),남성희 ( Sung Hee Nam ),정이연 ( I Yeon Jung ),성규병 ( Gyoo Byung Sung ),남학우 ( Hack Woo Nam ),장승종 ( Seung Jong Chang ),허현 ( Hyeon Hyur ),이민웅 ( Min Woong Lee ),곽순성 ( Shun Xing Guo ) 한국잠사학회 2003 한국잠사곤충학회지 Vol.45 No.2

천마 종자의 발아 조건에 관한 연구

홍인표 ( In Pyo Hong ),남성희 ( Sung Hee Nam ),정이연 ( I Yeon Jung ),성규병 ( Gyoo Byung Sung ),남학우 ( Hack Woo Nam ),정종천 ( Jong Chun Cheong ),박정식 ( Jeong Sik Park ),허현 ( Hyeon Hur ),이민웅 ( Min Woong Lee ) 한국균학회 2004 韓國菌學會誌 Vol.32 No.1

Optimization of Mycelial Growth of Entomogenous fungi of the Genus Cordyceps

홍인표,남성희,정이연,성규병,남학우,강석우,허현,이민웅,곽순성,Hong, In-Pyo,Nam, Sung-Hee,Jung, I-Yeon,Sung, Gyoo-Byung,Nam, Hack-Woo,Kang, Seok-Woo,Hur, Hyeon,Lee, Min-Woong,Guo, Shun-Xing The Korean Society of Mushroom Science 2004 한국버섯학회지 Vol.2 No.3

본 연구는 동충하초속균의 자실체 인공 재배를 위한 기초자료를 얻고자 제주도 한라산에서 채집한 Cordyceps longissima, C. militaris, C. pruinosa 등 야생 동충하초의 형태적, 생리적 특성조사를 수행하였다. Cordyceps longissima : 지좌는 숙주에서 1~2개 발생하며 곤봉형 또는 불규칙형으로 표면이 다소 거칠고 적갈색을 띤다. 두부는 $23{\sim}59{\times}6{\sim}8mm$이며, 병부는 원통형으로 $37{\sim}151{\times}2.5{\sim}4.0mm$이다. 자낭각은 묻힌형으로 $553{\sim}600{\times}215{\sim}270{\mu}m$. 자낭은 가는 원통형으로 $330{\sim}510{\times}5{\sim}6{\mu}m$이며, 자낭포자는 $11{\sim}13{\times}1.4{\sim}1.8{\mu}m$이다. 자실체는 매미목 매미과의 곤충에서 발생하였다. 균사 생장은 MCM 배지에서 $25^{\circ}C$, 4주 배양시 73 mm이며, 균사의 색택은 진황색을 나타내었다. 탄소원으로는 단당류인 glucose, 질소원으로는 potassium nitrate를 가장 잘 이용하였다. C. militaris : 지좌는 숙주에서 1~3개 발생하며 곤봉형으로 주황색을 띤다. 두부는 $9{\sim}23{\times}6{\sim}8mm$이며, 병부는 원통형으로 $27{\sim}30{\times}6{\sim}7mm$이다. 자낭각은 반묻힌형으로 $465{\sim}510{\times}260{\sim}310{\mu}m$. 자낭은 가는 원통형으로 $320{\sim}385{\times}4.2{\sim}4.8{\mu}m$이며, 자낭포자는 $2.2{\sim}4.5{\times}1.2{\sim}1.4{\mu}m$이다. 자실체는 나비목 곤충의 번데기에서 발생하였다. 균사 생장은 PDA 배지에서 $25^{\circ}C$, 4주 배양시 71 mm이며, 균사의 색택은 연노랑색을 나타내었다. 탄소원으로는 단당류인 frucose, 질소원으로는 potassium nitrate를 가장 잘 이용하였다. C. pruinosa : 지좌는 숙주에서 1개 발생하며 곤봉형으로 주홍색을 띤다. 두부는 $7{\sim}9{\times}3{\sim}4$이며, 병부는 원통형으로 $13{\sim}22{\times}2{\sim}3mm$이다. 자낭각은 반묻힌형으로 $350{\sim}520{\times}130{\sim}310{\mu}m$. 자낭은 원통형으로 $256{\sim}270{\times}5{\sim}6{\mu}m$이며, 자낭포자는 $3.2{\sim}5.1{\times}0.8{\sim}1.3{\mu}m$이다. 자실체는 나비목 곤충의 번데기에서 발생하였다. 균사 생장은 PDA 배지에서 $25^{\circ}C$, 4주 배양시 60 mm이며, 균사의 색택은 연붉은색을 나타내었다. 탄소원으로는 단당류인 arabinose와 mannose, 질소원으로는 sodium nitrite를 가장 잘 이용하였다. This study was carried out to obtain basic data on physiological characteristics for an artificial cultivation of fruiting body of Cordyceps. Specimens such as Cordyceps longissima, C. militaris and C. pruinosa were collected at Mt. Halla of Cheju island in July, 2003. Among four different culture media which have been used for culture of mushrooms, MCM medium was selected for the favorable culture medium of the Cordyceps tested. The initial pH of solid medium for mycelial growth of Cordyceps was good in the range of pH 5.0~7.0 lower than 8.0. The mycelial growth of C. longissima was most favorable on culture media supplemented with glucose, one of monosaccharides. In C. militaris, nine carbon sources were favorable to the mycelial growth as compared with control among 11 carbon sources. Six nitrogen sources were favorable to the mycelial growth of C. longissima as compared with control among 9 carbon sources; namely, the mycelial growth of C. longissima was most favorable on culture media contained potassium nitrate, and followed in order by ammonium citrate and sodium nitrate in 4 weeks incubation.

Leaching Model System 에 의한 토양 정균현상의 분석

홍인표,이민웅,허현 한국균학회 1987 韓國菌學會誌 Vol.15 No.4

This study was carried out to investigate the germination of four fungi under non-leaching and leaching incubation. The range of germination rates of Fusarium solani f. sp. pisi, Fusarium oxysporum f. sp. cucumerium, Mortierella, and Helminthosporium sativa incubated on sand saturated with various solutions without leaching were 90-99%, 31-45%, 38-62% and 86-98% respectively. The range of germination rates of Fusarium solani f. sp. pisi, Fusarium oxysporum f. sp. cucumerium, Mortierella n. sp and Helminthosporum sativa incubated on sand undergoing leaching were 51-63%, 13-25%, 15-37% and 47-80%, respectively. The germination rates of the four fungi tested were suppressed under leaching incubation.

복령 및 인삼추출물이 Sarcoma-180 Mouse 의 혈액상에 미치는 영향

홍인표,김동원,이민웅,이복임 한국균학회 1990 韓國菌學會誌 Vol.18 No.4

This study was made to observe changes in hemograms of the Sarcoma-180 mouse treated with extracts of Poria cocos and Panax ginseng. The life span of the Sarcoma-180 mouse administered with the mixture of the extract of Panax ginseng with the extract of P. cocos and the extract of P. cocos was extended more than the non-treated group. In the body weight of the Sarcoma-180 mouse, the non-treated group was increased more than the normal group and the groups administered with the mixture of ginseng extract with hoelen extract and hoelen extract. In the numbers of white blood cell especially lymphocyte, the groups administered with the mixture of ginseng extract with hoelen extract and hoelen extract were similar to the normal group, but the non-treated group was decreased. The Hematocrit, mean corpuscular volume and mean corpuscular hemoglobin in the mice infected with Sarcoma-180 were not significantly different between the group treated with extracts of them and the non-treated group. In the distributions and sizes of white blood cell and platelet in the mice, the group administered with the Hoelen extract was similar to the normal group comparing to the non-treated group.

복령의 형태 및 균사배양에 관한 연구

홍인표,이상선,이민웅,김광포 한국균학회 1991 韓國菌學會誌 Vol.19 No.1

In the traditional medicine, the basidiocarps of Poria cocos (Fr.) Wolf naturally collected have been widely used as a traditional remedy for dropsy, diarrhea, gonorrhea and nervous disease more than 3500 years ago. It was recently found to be composed of the particular compounds having antitumor effects. Therefore, its demand has been gradually increased, whereas it is still dependent upon the natural harvest. The artificial cultivation of P. cocos was carried out to observe the morphological characteristics and to investigate the cultural characteristics of P. cocos isolates collected. The morphological characteristics of its basidiocarps was observed. The physiological aspects of its isolates were also investigated.

A Study on the Selection of Medium for Chlamydospore Formation in Fusarium spp.

Hong, In-pyo,Lee, Min-woong 동국대학교 농림과학연구소 1986 農林科學 論文集 Vol.11 No.-

Fusarium spp의 4菌株(F. solani, F. solani f. sp. pisi, F. oxysporum f. sp. pisi and F. oxysporum f. sp. niveum)를 使用하고 6가지의 각기 다른 培地를 만들어 접종 7日後에 厚膜胞子 形成率을 調査하였다. 使用된 培地 中 殺菌土壤 抽出液은 Fusarium 菌屬에 關係없이 約 80%以下의 厚膜胞子 形成率을 나타내어 가장 좋았으나, Phosphage buffer solution과 滅菌數는 8%內外의 形成率을 나타내어 가장 低調하였다. 0.01M NaCl에서는 F. solani 菌屬이 F. oxysporum 菌屬보다 厚膜胞子 形成率이 좋았으나, 0.03M Na_2SO_4 培地에서는 F. oxysporum이 F. solani보다 約 15% 높은 形成率을 보였다. 0.01M NaCl과 0.03M Na_2SO_4의 混合培地에서는 60%以上의 厚膜胞子 形成率을 보여 使用된 培地 中 殺菌土壤 抽出液 다음으로 좋은 培地였다. Induction of chlamydospore formation was investigated for Fusarium solani, F. solani f. sp. pisi, F. oxysporum f. sp. pisi and F. oxysporum f. sp. niveum. Among the tested media sterile soil extract was excellent for chlamydospore formatioin-F. solani formed chlamydospores about 80%, F. solani f. sp. pisi 87%, F. oxysporum f. sp. pisi 76%, and F. oxysporum f. sp. niveum 87%, while phosphate buffer (0.05M) and sterile distilled water were poor as about 8% information of chlamydospores irrespective of Fusarium ssp. F. solani was better than F. oxysporum in forming chlamydospores in 0.01M NaCl condition, but in 0.03M Na_2SO_4 this result was reversed. Addition of 0.01M NaCl to 0.03M Na_2SO_4 increased the number of chlamydospore formation because it was formed chlamydospores above 60%. This result showed that mixture of the two media was excellent for chlamydospore formation next sterile soil extract.