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BHA 와 NaCl 의 혼합첨가에 의한 미생물의 생육저해효과
윤원호,양종범,김창한 ( Won Ho Yoon,Jong Beon Yang,Chang Han Kim ) 한국축산학회 1985 한국축산학회지 Vol.27 No.1
These experiments were carried out to investigate the growth inhibition of Staphylococcus aureus, Escherichla coli and Pseudomonas fluorescens, respectively, in nutrient broth, and total viable cell counts in meat, using combination of butylated hydroxyanisole(BHA) and sodium chloride(NaCl). The results obtained were summarized as follows: 1. In the bacteria, Staphy. aureus was the most sensitive especially, bactericidal effect was shown at 200ppm BHA. With 5%-, 7%-NaCl and 50ppm BHA, a bacteriostatic effect was observed at 18, 12hr, respectively. 2. Cell numbers of E. coli increased at 200ppm BHA in the absence NaCl, but bactericidal effect was perceived at 5%-NaCl and 200ppm BHA, as well as, 7%-NaCl and 100ppm BHA. 3. The effect of bactericide on Ps. fluorescens was not present at below 200ppm BHA without NaCl, but at any level of BHA with above 5%-NaCl. 4. The addition of BHA and NaCl was effective in the prevention of total viable cell counts in raw meat (pork).
국내 소비 주요 생약재의 유기용매 추출물에 대한 유전독성평가
윤원호,Yoon, Won Ho 한국식품영양학회 2013 韓國食品營養學會誌 Vol.26 No.4
This study is carried out to evaluate the genotoxicity of herbs (Angelica decursiva, Polypori umbellate, Astragalus membranaceua, Paeonia iactiflora, Glycyrrhiza uralensis, Cnidium officinale, Rehmannia glutinosa, Cyperus rotundus, Dioscorea batatas and Platycodi Radix) by using the Ames test. The Salmonella typhimurium reversion assay is being performed by using the Sal. typhimurium TA98, TA100, TA102, TA1535 and TA1537 as tester strains. Among 70% of ethanol extracts from 10 herbs, the number of revertant colonies is being increased in Astragalus membranaceua, Cnidium officinale and Dioscorea batatas in a dose-dependent manner, as compared with negative controls of the metabolic activation. In case of dichloromethane and ethyl acetate fraction from 70% of ethanol extracts, the number of revertant colonies is increased in Angelica decursiva, Astragalus membranaceua, Cnidium officinale, Rehmannia glutinosa and Dioscorea batatas in a dose-dependent manner, as compared with negative controls of metabolic activation. Such results indicate that Angelica decursiva, Astragalus membranaceua, Cnidium officinale, Rehmannia glutinosa and Dioscorea batatas all show genotoxic effects when being extracted with the solvent extractions such as 70% of ethanol, dichloromethane and ethyl acetate, and thus, they might be genotoxically- non-safe.
윤원호,최재훈,이경호,김창한,Yoon, Won-Ho,Choi, Jae-Hoon,Lee, Keyong-Ho,Kim, Chang-Han 한국식품과학회 2005 한국식품과학회지 Vol.37 No.1
본 연구는 폐기되는 차나무 열매를 이용하여 향균활성과 항종양활성을 비교하고 이용가능성에 대하여 탐색하고자 하였다. 항균활성과 항종양활성을 알아보기 위하여 70% 에탄올과 물 추출물의 활성을 측정하였다. 항균활성은 식중독균과 몇 종의 병원균, 효모, 곰팡이 등 17균주에 대하여 추정하였고, 항종양 활성은 6종류의 인체 암세포주, 1종류의 정상세포주에 대하여 조사하였다. 차나무 잎, 종자와 과피의 항균활성을 비교한 결과는 종자의 70% 에탄올과 물 추출물에서 효모에 대하여 항균활성이 높게 나타났다. 특히, candida albicans, Cryptococcus neoformans에 대하여 항균활성을 보였다. 종자 물 추출물을 $80,\;100^{\circ}C$에서 30분, $121^{\circ}C$에서 15분간 열처리 후 항균활성을 검색한 결과 가열온도에 따른 차이는 크게 나타나지 않았다. 항종양 활성을 검색하기 위하여 MTI assay를 통해 활성을 측정하였다. 과피 추출물에서 전혀 활성이 없었지만 종자 추출물에서는 큰 활성을 나타내었다. 극성용매인 70% 에탄올과 물 추출물의 종양세포 증식억제효과가 높게 나타났으며 그중에서 물 추출물이 70% 에탄올 추출물 보다 높게 항종양 활성이 나타났다. 차나무 종자로부터 추출한 활성물질의 종양세포와 정상 세포에 대한 세포독성을 MTT assay로 비교한 결과 $50{\mu}g/mL$의 농도에서 난소암 세포인 SK-OV-3는 70%의 억제율을 나타낸 반면 정상세포에서는 10% 미만으로 나타나 정상세포보다 종양세포에 대하여 보다 큰 선택 독성을 나타내는 것으로 보인다. Antimicrobial and antitumor activities of Camellia sinensis L seed extracts were investigated. Seed extracts showed antifungal activities against Candida albicans IFO 1594 and Cryptococcus neoformans. Inhibition zone of 20 mm was shown by 70% ethanol extract against C. albicans IFO 1594 at 100 mg/mL. Antifungal activity of seed extract was not decreased by heating at 80 and $100^{\circ}C$ for 30 min or at $121^{\circ}C$ for 15 min, indicating heat-stability of seed component. Growth-inhibitory effects were observed in 70 and 10% of tumor cell line SK-OV-3 and normal ceil line NIH/3T3 at $50{\mu}g/mL$, respectively.
윤원호,황진용,김창한,Yoon Won-Ho,Hwang Jin-Yong,Kim Chang-Han 한국축산식품학회 2004 한국축산식품학회지 Vol.24 No.3
This study was carried out to investigate the antitumor activity from sulfur fed duck. The antitumor substances were crude purified by solvent extraction, silica gel column chromatography, and HPLC using C18 column. In MTT assay, the active compounds exhibited more cytotoxic activity on tumor cell lines than normal cell line. In addition of 100 $\mu\textrm{g}$/mL concentrations of crude purified active compounds, the growth inhibition rate of tumor cell lines was 56% (Hep-2j human larynx), 58% (KB; human epidermoid of mouth carcinoma), and 28% (MDBK; bovine normal kidney), respectively. The survival rate of clonogenic assay was 26% in Hep-2 and 28% in KB at 200 $\mu\textrm{g}$/mL. 본 연구에서는 유황오리로부터 항종양 효과를 나타내는 물질을 용매 추출법과 각종 크로마토그래피를 사용하여 분리 및 정제하였다. 유황오리로부터 정제한 활성 물질의 각종 종양세포에 대한 항암 효과를 측정한 결과 MTT assay는 100 $\mu\textrm{g}$/mL의 농도에서 HEp-2는 56%, KB는 58%의 세포 증식 억제 효과가 나타났다. 정상 세포주인 MDBK는 28%의 세포증식억제 효과가 나타나 정상 세포에 대한 세포 독성은 나타나지 않았다. Clonogenic assay는 200 $\mu\textrm{g}$/mL의 농도에서 HEp-2는 26%, KB는 28%의 생존율을 나타내었다. 따라서 유황오리로부터 정제한 활성 물질은 후두암 세포주인 HEp-2와 구강암 세포주인 KB에서만 특이적으로 세포 증식 억제 효과를 나타내었다.
Lactobacillus bulgaricus와 Kluyveromyces marxianus의 혼합 스타터를 이용한 기능성 발효유의 특성
윤원호,남보라,김진만,김창한,Yoon Won-Ho,Nam Bo-Ra,Kim Jin-Man,Kim Chang-Han 한국축산식품학회 2006 한국축산식품학회지 Vol.26 No.2
본 연구는 기능성 발효유로서 티벳산 발효유에서 분리한 효모(Kluyveromyces marxianus)와 유산균(Lactobacillus bulgaricus)의 혼합스타터를 이용한 발효유를 제조하여 배양 기간별 균수 측정, pH, 적정산도, ethanol 함량, 항암 활성에 대해 알아보았다. 배양은 $30^{\circ}C$에서 curd가 형성되는 시점에 마치게 되는데 이때의 최종 산도는 0.68, pH는 4.5 이었다. 배양 36시간 후의 균수는 K. marxianus는 $5.3{\times}10^9 CFU/mL$, L. bulgaricus는 $3.2{\times}10^9 CFU/mL$ 이었고 ethanol 함량은 0.35%까지 증가하였다. 36시간 배양하여 제조된 발효유의 항종양 활성은 HEp-2에 대해서는 86.6%, HEC-1B에 대해서는 70.3%, SW-156에 대해서는 60.4%, SK-MES-1에 대해서는 57.14%의 항종양 활성을 나타내었다. 이상의 결과 기존의 유산균 발효유에 효모를 첨가한 알코올 발효유의 제조가 가능하며, 제품의 항종양 활성의 측면에서도 높은 기능성을 나타내는 것으로 나타났다. This study was carried out to investigate characteristics of acid and alcohol fermented milk by mixed starters made by Lactobacillus bulgaricus (KCTC 3635) and Kluyveromyces marxianus (KCTC 17212) for 36 hours when the curds were formed. Final pH and titratable acidity were about 4.5 and 0.68%, respectively. The viable cell counts of lactic acid bacteria and yeast for alcohol fermented milk were increased to $3.2{\times}10^9 CFU/mL$ and $5.3{\times}10^9 CFU/mL$, respectively. The ethanol contents increased to 0.35% during fermentation. Antitumor activities of the fermented milk against tumor cell lines, such as HEp-2, HEC-1B, SW-156 and SK-MES-1 showed to 86.6, 70.3, 60.4 and 57.14%, respectively.
루테인과 후코이단 병용 처리에 의한 AAPH 유도 세포 손상 억제
이경호 ( Keyong Ho Lee ),윤원호 ( Won Ho Yoon ) 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.3
This study was designed to investigate the protective effect of the combination of fucoidan and lutein against AAPH-induced oxidative stress in THP-1 cells. The combination of fucoidan and lutein existed significant antioxidant effect on AAPH-damaged THP-1 cells by using lipid peroxidation and cellular antioxidant capacity assay. Fucoidan(1㎍/㎖) and lutein(10㎍/㎖) did not affect at all the viability of THP-1 cells, but protected the AAPH-damage of THP-1 cells at the same concentration. The viability of THP-1 cells was 0% with 1 mM AAPH alone, the protective effect of fucoidan(1㎍/㎖) and lutein(10㎍/㎖) was 37% and 36%, respectively. The combination of fucoidan(1㎍/㎖) and lutein(10㎍/㎖) exhibited significant inhibitory effect of lipid peroxidation using TBARS assay and cellular antioxidant capacity using DCFH-DA assay. In lipid peroxidation, the TBARS value of 1mM AAPH alone was 0.8±0.03nM MDA, its of the combination of fucoidan(1㎍/㎖) and lutein(10㎍/㎖) was 0.2±0.05nM MDA. In cellular antioxidant capacity, the combination of fucoidan(1㎍/㎖) and lutein(10㎍/㎖) exhibited significant cellular antioxidant capacity of 76%, whereas quercetin(10μM) as positive control exhibited the cellular antioxidant capacity of 32%. These results indicate that the cotreatment of fucoidan and lutein protects against AAPH-induced THP-1 cell damage by inhibiting lipid peroxidation, increasing cellular antioxidant capacity.