금연침에 대한 반응군과 비반응군의 유전자 다형성 차이 연구

윤동학,박히준,김승태,진수희,이수진,이혜정,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

Objectives : This study was designed to investigate the differences of effects in smoking cessation after acupuncture treatment and we hypothesized that the discrepancies might be caused by individual genetic differences. Methods : Acupuncture treatment was given to the subjects three times a week for the 231 healthy male Korean smokers without personal or familial history of psychiatric or neurological illness. We evaluated for differentiate responder and non-responder who showed more than 50% decrease in the cigarette consumption or the desire for smoking were regarded as responder, and less than 25% decrease in the cigarette consumption or the desire for smoking were regarded as non-responder, respectively. Allele and genotype frequencies of the Taq1 A polymorphism of dopamine D2 receptor (DRD2) gene were compared in 231male smokers. Chai-square analyses were performed to test for an interactive effect between the DRD2 Taq1 A allele. Results : The allele frequencies and genotype distributions of DRD2 gene among the smokers (n = 231) showed significant the differences in their genotype distributions. The responder and non-responder showed the difference in genotype distribution with a prevalence of A1 allele. A slightly positive association of DRD2 Taq1 A1 genotypes with smoking was observed. Conclusions : This experiment results indicate that the present of DRD2 allele genotype showing significant difference in the genotype distributions between responders and non-responders could be explained by the difference in the genetic effect of DRD2 A1 allele.

금연침에 대한 반응군과 비반응군의 유전자 다형성 차이 연구

윤동학 ( Dong Hak Youhn ),박히준 ( Hi Joon Park ),김승태 ( Seung Tae Kim ),진수희 ( Soo Hee Jin ),이수진 ( Su Jin Lee ),이혜정 ( Hye Jung Lee ),임사비나 ( Sa Bi Na Lim ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.1

Objectives: This study was designed to investigate the differences of effects in smoking cessation after acupuncture treatment and we hypothesized that the discrepancies might be caused by individual genetic differences. Methods: Acupuncture treatment was given to the subjects three times a week for the 231 healthy male Korean smokers without personal or familial history of psychiatric or neurological illness. We evaluated for differentiate responder and non-responder who showed more than 50% decrease in the cigarette consumption or the desire for smoking were regarded as responder, and less than 25% decrease in the cigarette consumption or the desire for smoking were regarded as non-responder, respectively. Allele and genotype frequencies of the Taq1 A polymorphism of dopamine D2 receptor (DRD2) gene were compared in 231male smokers. Chai-square analyses were performed to test for an interactive effect between the DRD2 Taq1 A allele. Results: The allele frequencies and genotype distributions of DRD2 gene among the smokers (n=231) showed significant the differences in their genotype distributions. The responder and non-responder showed the difference in genotype distribution with a prevalence of A1 allele. A slightly positive association of DRD2 Taq1 A1 genotypes with smoking was observed. Conclusions: This experiment results indicate that the present of DRD2 allele genotype showing significant difference in the genotype distributions between responders and non-responders could be explained by the difference in the genetic effect of DRD2 A1 allele.

ISOFIX방식에 따른 어린이 탑승자 안전도 연구

김동학(Donghak Kim),윤영한(Younghan Youn),이재완(Jaewan Lee) 대한기계학회 2002 대한기계학회 기타 간행물 Vol.2002 No.11

보행자에 대한 프라스틱 범퍼 가드의 안전도 분석

윤영한(Younghan Yoon),김동학(Donghak Kim),양재의(Jaeeui Yaung),이정선(Jeongsun Lee) 한국자동차공학회 2003 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

Safety evaluation of the frontal attachment of the car needs to decrease the number of pedestrian casualty from car accident since increasing of SUVs which have a steel bull bar and a plastic bumper guard. The pedestrian injury risk can be evaluated by headform, legform and upper legform according to the European Enhanced Vehicle-Safety Committee(EEVC) Working Group Report. The EEVC proposed three sub-system tests: headform impact to bonnet top, legform to bumper and upper legform to bonnet leading edge.<br/> In this study, Bumper guard attached vehicle's pedestrian safety was evaluated by child headform and legform test relevant to the EEVC Working Group Report. In addition to this test, FEM analysis was used to improve the safety of the plastic bumper guard for the SUVs.<br/>

ISOFIX방식에 따른 어린이 탑승자 안전도 연구

김동학(Donghak Kim),윤영한(Younghan Youn),이재완(Jaewan Lee) 한국자동차공학회 2002 한국자동차공학회 Symposium Vol.2002 No.11

To impreve Child Occupant safety, US adopt a requirement for universal, uniform anchor systems for child restraints, an enormous effort of some child seat manufacturers tried to improve the protective properties of Child Restraint System(CRS). The new standard includes FMVSS 225, Child Restriant Anchorage Systems and Amendments to FMVSS 213 came into effect in 1999. The rules of universal anchor are to provide devices which are independent of safety belts to attach child restraints to the vehicle. It will allow child restraints are anchored more securely than those are often possible with current safety belts. Recently, US, Australia and Euripean community countris including Japan have started testing of evaluation of CRS performances during their NCAP test programs and results were published.<br/> Koran govemment realized the importacne of CRS roles in child safety and investigate benefits of enhancements of CRS regulations. In this study, four kinds of ISOFIX were simulated by commercial program as well as the current CRS and were evaluated in order to verity their safety for Child Occupant.

승합차의 Bullbar 부착에 대한 보행자 안전도 비교연구

윤영한(Younghan Youn),김동학(Donghak Kim),홍재수(Jaesoo Hong),최영태(Youngtae Choi),윤경한(Kyunghan Yoon) 한국자동차공학회 2002 한국자동차공학회 춘 추계 학술대회 논문집 Vol.2002 No.5_2

This study investigates the positive and negative aspects of bullbar with regard to road trauma through assessment of the headfoam impact test and analysis of pedestrian crash.<br/> The experimental data showed that bullbars pose a great risk in the event of low speed impacts with unprotected road users such as pedestrians.<br/> All of steel bullbar tested have been shown to have a high risk of causing life threatening brain injuries in impacts with the head of children or serious injuries to the abdomen and chest of adults and taller children, even in low speed impact.<br/> Despite the fact that safety appears to be a major reason for fining bullbars, no results were found which attempted to quantify the safety benefits of bullbar.<br/>

의학입문에 수록된 금침혈 연구

채광민,권선오,윤동학,김승태 경락경혈학회 2019 Korean Journal of Acupuncture Vol.36 No.3

Objectives : The authors aimed at contributing to the safety of acupuncture in the clinic by analyzing the information of contraindicated acupoints described in Uihakipmun. Methods : We investigated the contents related acupuncture contraindications in Uihakipmun, categorized the contraindicated acupoints by site and identified whether they can cause adverse events from the anatomical point of view. Results : In Uihakipmun, 49 acupoints - BL6, BL8, BL9, ST1, SI18, BL1, BL2, TE18, TE19, TE20, GB1, GB3, GV22, GV24, ST12, ST9, TE16, GV6, BL15, BL30, CV17, LU2, GB22, SP16, CV8, CV9, CV15, KI11, ST30, SP11, BL56, GB32, GB33, GB42, LI13, HT2, TE8, GV17, GB18, EX-HN11, GB21, GV11, GV10, ST17, CV5, CV1, ST42, SP6, LI4 - were described as contraindicated acupoints. Among them, the contraindications induced by acupuncture treatment were described for just 5 acupoints. Anatomically, acupuncture stimulation at the contraindicated acupoints can cause organ, nerve or vessel damage near the acupoints. And the number of contraindicated acupoints in Uihakipmun was increased compared to those in Hwangjenaegyeong, Chimgugabeulgyeong, Bigeupcheongeumyobang, Donginsuhyeolchimgudogyeong and Chimgujasaenggyeong. Conclusions : In Ming dynasty, the knowledge associated with adverse events on acupuncture was increased by the accumulation of medical knowledge. Acupuncture stimulation at the contraindicated acupoints described in Uihakipmun can cause tissue damage, therefore we should perform acupuncture procedure carefully to avoid adverse events when stimulating the contraindicated acupoints.

천마의 흑질 내 도파민성 신경세포 보호 효과에 대한 단백체학적 분석

배창환,김희영,이한울,서지은,윤동학,김승태 경락경혈학회 2022 Korean Journal of Acupuncture Vol.39 No.4

Objectives : Parkinson’s disease (PD) is a neurodegenerative disorder threatening the quality of life and highly occurred in over 65 years old. Gastrodia elata Blume (GEB), a traditional medicine used for the treatment of headache and convulsion, has been reported to have neuroprotective effect. This study was designed to investigate the neuroprotective effect of GEB and the proteomic changes in the substantia nigra (SN) of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. Methods : Male eleven-week-old C57BL/6 mice were intraperitoneally injected with 30 mg/kg of MPTP at 24-h intervals for 5 days. Two hours after the daily MPTP injection, the mice were orally administered 800 mg/kg of GEB extract, which continued for 7 days beyond the MPTP injections, for a total of 12 consecutive days. Two hours after the final GEB administration, the brain samples were collected, and dopaminergic neuronal death and proteomic changes in the SN were evaluated. Results : GEB prevented the MPTP-induced dopaminergic neuronal death and regulated the expression of 11 proteins including thimet oligopeptidase, T-complex protein 1, glycine tRNA ligase, and pyruvate kinase isozymes M1. Conclusions : GEB prevents MPTP-induced dopaminergic neuronal death by regulating the proteins in the SN.

MPTP로 유도된 파킨슨병 생쥐 모델에 대한 시호계지탕, 시호소간탕, 시호청간탕의 신경세포 보호 효과 비교 연구

서지은,이한울,배창환,윤동학,김희영,김승태 경락경혈학회 2023 Korean Journal of Acupuncture Vol.40 No.3

Objectives : Parkinson’s disease (PD) is the second most common neurodegenerative disorder worldwide and is characterized by the loss of the dopaminergic neurons in the substantia nigra (SN). In a previous in vitro study, we demonstrated that Sihogyeji-tang (SG), Sihosogan-tang (SS), and Sihocheonggan-tang (SC) have the potential to be candidate medicines for PD. This study aimed to compare the neuroprotective effect of SG, SS, and SC using 1-methyl-4-phenyl-1,2,3,6-tetrahydrophridine (MPTP)-induced PD mouse model. Methods : Eight-week-old male C57BL/6 mice were intraperitoneally administered with 30 mg/kg of MPTP for 5 days and orally administered SG, SS and SC for 12 days from the first MPTP injection. Motor function was assessed using the pole test and the rotarod test. Dopaminergic neuronal survival in the SN and striatum was evaluated through tyrosine-hydroxylase immunohistochemistry. Results : MPTP administration resulted in behavioral impairment and dopaminergic neuronal death in the SN and striatum. In the pole test, treatment with SG, SS, and SC alleviated the MPTP-induced motor dysfunction on day 5 and 12. In the rotarod test, SS and SG alleviated the MPTP-induced motor dysfunction on day 5, while only SS showed improvement on day 12. SS and SG significantly protected dopaminergic neurons in the SN from MPTP toxicity, and all three compounds (SG, SS, and SC) showed significant protection in the striatum. Notably, SS demonstrated superior efficacy in suppressing MPTP-induced motor dysfunction and dopaminergic neuronal death compared to SG and SC. Conclusions : These findings suggest that SS is the most effective formula among SG, SS, and SC for PD, indicating its potential role in the treatment of PD.

足三里의 電鍼刺戟이 흰쥐의 中樞神經系에서 Interleukin-6의 活性에 미치는 影響 - 求心性 體感覺 情報傳達을 中心으로- : 求心性 體感覺 情保傳達을 중심으로

이혜정,신형철,진수희,손양선,윤동학,임사비나 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

Objectives : Acupuncture is expected to have somewhat like the efficacy parallel increasing activity of immune system in Western modern medicine. There, already, are many animal researches on activating effect of acupuncture for the immune system in peripheral organs. So, we carried out this experiment to see whether acupuncture has controlling effect on interleukin-6(IL-6) activity in rat's brain. Methods and Results : We had topical application of IL-6(1U-1pg, 10㎕) on brain of rat. It reduced afferent sensory transmission to the primary somatosensory(SI) cortex from periphery. Whereas, electrical stimulation(ES, 2Hz, 1.5V, 15min) of ST36(足三里) with application of IL-6 prominently activated afferent sensory transmission. ES of non-acupoint(proximal tail) with IL-6 showed suppression of afferent transmission. ES of ST36 without IL-6 application also exerted facilitation of afferent transmission to the SI cortex. Conclusions : Electoacupuncture(EA) on ST36 has noticeable influences on modulating activation of IL-6 in central nervous system, which do major role in immune system.