Evidence for Alkaline Phosphatase Involvement in tRNA-Stimulated Amino Acid Transport Systems

유승현,Yoo, Seung-Hyun 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.1

세포막 단백질 중의 하나인 periplasmic 단백질이 적절한 tRNA의 존재하에 아미노산 수송기작에 관여함이 알려졌는데, 이 단백질이 alkaline phosphatase로 밝혀졌다. 이 단백질의 분자량은 gel여과 방법으로는 88,000, SDS전기 영동으로는 43,500으로 나타났는데 두 개의 동일한 단일체로 존재함을 나타내었다. 아미노산 분석과 효소 역할에 의거하여 alkaline phosphatase임이 알려졌고, 적절한 tRNA와 더불어 아미노산 수송을 크게 증가 시킴을 볼 수 있었다. A periplasmic protein which later turned out to be alkaline phosphatase has been found to participate in amino acid transport mechanisms of Escherichia coli in the presence of appropriate tRNA. The apparent molecular weight of the purified protein was estimated to be 88,000 by gel filtration and 43,500 by SDS-polyacrylamide gel electrophoresis, indicating this protein is a dimer composed of two identical monomers. The purified protein which showed tRNA-dependent amino acid transport activity in the shocked E. coli 9723 cells has been identified as alkaline phosphatase based on its amino acid composition and enzymatic activity in addition to the molecular size. Purified alkaline phosphatase or tRNA alone did not exert any significant effect on leucine, isoleucine or valine transport, whereas simultaneous addition of these two molecules increased the amino acid transports drastically.

Roles of Nucleosides on Amino Acid Transport Systems of E. Coli

유승현,Yoo, Seung-Hyun 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.1

E. coli를 배양하여 삼투에 의한 충격으로 막 내의 단백질을 추출시킨 후, 이들 E. coli의 아미노산 수송에 관여하는 Nucleoside의 영향을 관찰하였다. Pyrimidine Nucleoside인 Cytidine과 Uridine이 아미노산 수송을 크게 증가시켰는데 Purine Nucleoside들은 효과가 조금 뒤졌다. 증가된 아미노산 수송은 주로 세포내의 아미노산 pool에 도착되었고, 단백질이나 RNA합성을 저지하여도 이 수송은 영향을 받지않았다. 이런 자극 효과는 Nucleoside의 독특한 효과에 의한 것으로 밝혀졌다. Transport of a number of amino acids in osmotically shocked cells of Escherichia coli after growth in enriched media has been found to be enhanced by nucleosides, particularly cytidine and uridine which are more effective than purine ribonucleosides. Deoxyribonucleosides have activity approximately equivalent to that of the corresponding ribonucleosides. The stimulatory activity is involved primarily, in increasing the expandable pool of amino acid within the cell and is not significantly affected by inhibitors of protein or RNA synthesis. The stimulatory effect is specific to nucleosides because neither ribose, deoxyribose and free bases nor pseudouridine had any effect.

공직기강의 개념에 관한 연구

유승현(Seung Hyun Yoo) 경인행정학회 2020 한국정책연구 Vol.20 No.2

공직기강의 개념 정립과 현실 적용은 공직자의 충실한 직무수행을 유도하기 위한 핵심 전제조건이다. 그럼에도 현재 공직기강의 개념은 학술적･법적으로 정립되어 있지 않은 실정이다. 이러한 문제인식 하에 본 논문은 공직기강의 이론적 기반과 제도적 적용을 분석하여 현대 행정환경에 적합한 공직기강의 개념을 정립하는 데 그 목적이 있다. 이를 위해 Wittgenstein(1968)의 ‘언어게임(language game)’ 이론에 기초하여 공직기강의 사전적 개념과 공직자의 역할이론 및 책임성 확장에 대한 이론적 검토를 기반으로 행정현실에서 적용될 수 있는 공직기강의 개념적 스펙트럼을 도출하는 한편, 역대 정권의 공직기강 개념 활용 실태 분석을 통해 현대 행정환경에 적합한 공직기강 확립의 접근방법을 모색하였다. 이를 통해 공직기강의 개념적 스펙트럼으로 법적 책임성 차원에서 “복무관리”와 “반부패”를, 그리고 윤리적 책임성 차원에서 “소극행정 근절”과 “적극행정”을 제시하였다. 또한 민주화가 진전되고 국민의 역량이 성숙되어 공공거버넌스의 형태가 법적/관료제적 거버넌스에서 성과중심적/관리적 거버넌스와 합의지향적/정치적 거버넌스로 변화됨에 따라 공직기강의 개념적 중점이 복무관리와 반부패와 관련된 법적 책임성에서 소극행정 근절과 적극행정과 관련된 윤리적 책임성으로 이전･확장되어 왔음을 확인하였다. 이에 기반하여 법적 책임성 차원에서는 개별적인 문제점 지적보다는 이를 유발하는 제도적･조직적 요인을 개선하는 데 역량을 집중하는 한편, 윤리적 책임성 차원에서는 고의나 중대한 과실이 없는 이상 공익 실현을 위해 적극적으로 직무를 수행한 것에 대해서는 책임을 묻지 않는다는 신뢰 형성에 노력을 집중할 것을 제안하였다. Establishing the concept of public official discipline and applying it to reality are key prerequisites for encouraging public officials to fulfill their duties. Nevertheless, the concept of public official discipline is not established academically and legally. This paper aims to establish the concept of public official discipline suitable for the modern administrative environment by analyzing the theoretical basis and institutional application of public official discipline. Based on the theory of language game by Wittgenstein(1968), It seeks to derive a conceptual spectrum of public official discipline that can be applied in administrative reality on the basis of the dictionary concept of public official discipline and theoretical review of the role theory and extension of accountability, and to suggest an approach to establish public official discipline suitable for the modern administrative environment through analysis of the actual use of the concept of public office discipline by previous administrations. According to the analysis, conceptual spectrum of public official discipline is classified as “office regulation” and “anti-corruption” in terms of legal accountability, and “eradication of passive administration” and “active administration” in terms of ethical accountability. In addition, as democratization progressed and the capacity of the people matured, the type of governance has changed from legal/bureaucratic governance to performance-oriented/administrative governance and consensus-oriented/ political governance, and the conceptual focus of public official discipline has been transferred and expanded from legal accountability to ethical accountability. Based on the result, it proposes to focus on improving the institutional and organizational factors that cause it rather than identifying individual problems in terms of legal accountability on building up trust that no one should be held accountable for actively performing their duties to realize the public interest unless there is a deliberate or gross negligence.

이문구 소설의 특성 연구 -『관촌수필』, 『우리동네』 두 연작 소설을 중심으로-

유승현 ( Seung Hyun Yoo ) 우리어문학회 2006 우리어문연구 Vol.27 No.-

This study aims for clarifying the narrating method of Lee, Mun-ku`s novel, especially Gwanchonsupil and Wuridongne. Previous studies on Lee, Mun-ku`s novel is separated in two ways. One is social and cultural approaches which is based on the view of realism theory. The other is formal and aesthetical approaches which is based on the view of stylistics. However the aspect both of them mention in common is the writer`s peculiarity as a storyteller. It means that his novels are not written in the way normal modern novels are written but written in their own ways. And this makes the specialty of the way of Lee, Mun-ku`s storytelling. Both Gwanchonsupil and Wuridongne show the farm villages as backgrounds. But the farm village one of them show us is very different from another one. The farm village in Gwanchonsupil is the narrater`s hometown and the place which reminds the narrater past days everyone had lived happily. Therefore this novel express reflection and attachment for past days strongly. Wuridongne describes the lives of the farm village people as the present age. So it tells us the problem of the farm village very vividly by Lee, Mun-ku`s own way of storytelling. The reason Gwanchonsupil and Wuridongne shows us very different aspects, even though they have same background, is they are written by different ways of storytelling. Yet these different two novels show us the author`s special attitude in common. We can find the aspect as analyzing the way of storytelling of these two novels. Many studies on Lee, Mun-ku`s novel mention about lack of the author`s critical mind. This is because of the attitude revealed by the way of storytelling. This study pays attention to this point.

건강보험 재정안정정책의 성과분석

유승현 ( Seung Hyun Yoo ),문상호 ( Sang Ho Moon ) 한국정책학회 2006 韓國政策學會報 Vol.15 No.3

십이지장으로 전이된 폐선암

유승현 ( Seung Hyun Yoo ),심윤수 ( Yun Su Sim ),이진화 ( Jin Hwa Lee ),심기남 ( Ki Nam Shim ),장중현 ( Jung Hyun Chang ),김윤경 ( Yoon Kyung Kim ),성순희 ( Sun Hee Sung ) 대한결핵 및 호흡기학회 2007 Tuberculosis and Respiratory Diseases Vol.63 No.3

A gastrointestinal metastasis from lung cancer is rare and is quite often found during an autopsy. A 58-year-old man was admitted with left upper back pain and a mass in the left upper lobe on the chest radiograph. The transbronchial lung biopsy revealed an adenocarcinoma of the lung. A gastroduodenoscopy was carried out to evaluate the indigestion and epigastric discomfort. The duodenofibroscopy revealed bulging mucosa on the minor papilla of the duodenum. A metastasis to the duodenum from an adenocarcinoma of the lung was diagnosed by a duodenofibroscopic biopsy of the bulging lesion. We report this rare case of an adenocarcinoma of lung with a metastasis to the duodenum. (Tuberc Respir Dis 2007; 63: 283-288)

CAI : CAE 를 넘어서

유승현(Seung-Hyun Yoo) (사)한국CDE학회 2005 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2005 No.1

E . Coli 아미노산 수송 기작에 관여하는 Nucleoside 의 역할

유승현 ( Seung Hyun Yoo ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.1

tRNA 의 자극을 받는 아미노산 수송 기작에 관여하는 Alkaline Phosphatase 의 증거

유승현 ( Seung Hyun Yoo ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.1

A periplasmic protein which later turned out to be alkaline phosphatase has been found to participate in amino acid transport mechanisms of Escherichia coli in the presence of appropriate tRNA. The apparent molecular weight of the purified protein was estimated to be 88,000 by gel filtration and 43,500 by SDS-polyacrylamide gel electrophoresis, indicating this protein is a dieter composed of two identical monomers. The purified protein which showed tRNA-dependent amino acid transport activity in the shocked E. coli 9723 cells has been identified as alkaline phosphatase based on its amino acid composition and enzymatic activity in addition to the molecular size. Purified alkaline phosphatase or tRNA alone did not exert any significant effect on leucine, isoleucine or valine transport, whereas simultaneous addition of these two molecules increased the amino acid transports drastically.

성과감사의 개념화

유승현 ( Seung Hyun Yoo ) 중앙대학교 국가정책연구소 2018 국가정책연구 Vol.32 No.3

우리나라는 책임추궁 방식의 합법성감사의 반발과 부작용 극복 차원에서 합법성감사의 토대 위에 성과감사를 도입함으로써 성과감사와 합법성감사의 개념적 혼란 문제가 지속적으로 제기되어 왔다. 이는 성과감사를 체계적으로 발전시키고 그 실효성을 확보하는 데 큰 장애요인으로 작용한다. 이러한 문제인식하에 본 논문은 성과감사의 이론적 기반과 제도적 적용을 분석하여 현대 감사환경에 적합한 성과감사의 개념을 정립하는 데 그 목적이 있다. 이를 위해 Wittgenstein(1968)의 ‘언어게임(language game)’ 이론에 기초하여 성과감사 도입의 환경적 요인에 대한 검토, 그리고 성과감사라는 용어의 구성요소인 ‘성과’와 ‘감사’의 사전적 정의와 그 이론적 의미에 대한 고찰을 기반으로, INTOSAI와 미국과 영국 등 해외 주요국 최고감사기구를 대상으로 감사종류 및 성과감사의 개념, 기준 및 범위, 접근방법 등 제도적 적용실태를 분석함으로써 제도운영의 공통적인 규칙을 탐색하였다. 이를 통해 현대 감사환경에 적합한 성과감사의 개념적 요건으로 ① “목표달성과 관련된 핵심사항을 대상으로 하는 감사”, ② “간결하고 명확한 메시지를 전달하는 감사”, ③ “판단기준에 따른 감사결과를 균형있게 제시하는 감사”, ④ “미시적·개별적 조치보다는 거시적·구조적 개선을 추구하는 감사”를 제시하는 한편, 그 개념적 기초에 기반하여 우리나라 감사원의 향후 성과감사 발전방향을 제안하였다. In Korea, the problem of conceptual confusion between performance audit and compliance audit has been continuously raised by introducing performance audit on the basis of compliance audit in order to overcome negative side effects of compliance audit. This is a major obstacle to the development of performance audit. The purpose of this paper is to analyze the theoretical basis and institutional application of performance audit, and to conceptualize of performance audit to make it suitable for recent audit environment. It applied ‘language game’ theory (Wittgenstein, 1968) to conceptualization methodology, and reviewed the environmental factors of performance audit introduction, the dictionary definition and the theoretical meaning of 'performance' and 'audit' which are components of performance audit. Based on this reviews, it searched the common rules in reality by analyzing the institutional application of INTOSAI and the supreme audit institutions of major countries such as USA and UK. According to the analysis, the conceptual requirements suitable for recent audit environment include: 1) an audit that focuses on key issues in terms of achieving the goal; 2) an audit that conveys a concise and clear message; 3) an audit that presents audit results in a balanced manner based on audit criteria; and 4) an audit that pursues structural improvement rather than individual correction. Based on the conceptual basis, it proposes the direction of future performance audit.