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황형서 ( Hyung Seo Hwang ),유대성 ( Dae Sung Yoo ),심중현 ( Joong Hyun Shim ) 대한화장품학회 2016 대한화장품학회지 Vol.42 No.1
바나나잎의 기능성화장품 소재로써 응용 가능성을 규명하고자 바나나잎 추출물이 피부 미백에 미치는 효과를 B16F10 세포주를 활용하여 cell viability, mRNA 발현 및 tyrosinase (Tyr) 활성 저해 실험을 통하여 알아보았다. 10%의 바나나잎 추출물은 tyrosinase 활성을 저해하고, 멜라닌 합성을 65% 감소시키는 것을 확인하였다. 정량적 real-time RT-PCR을 수행한 결과 바나나잎 추출물이 tyrosinase related protein 1 (TRP1)/ tyrosinase related protein 2 (TRP2) / tyrosinase mRNA 발현을 각각 20 / 40 / 60% 가량 억제하는 효과를 확인하였다. 또한 바나나잎 추출물이 함유된 크림제형의 임상시험을 수행하여 자외선 조사 직후 바나나잎 추출물이 함유된 크림의 육안평가 결과 유의한 미백효과가 있는 것을 확인하였다. 본 연구 결과는 바나나잎 추출물이 미백 기능성 화장품 소재 뿐 아니라 향후 기전 연구 수행을 통해 다양한 산업화 소재로 개발될 수 있음을 시사한다. This research was carried out to identify the whitening effect of Banana leaf extract. B16F10 cells were used to measure cell viability, mRNA expression, and tyrosinase activity inhibition assay from B16F10 cell. We also carried out clinical test of the cream product containing banana leaf extract. In this study, we elucidated the effects of banana leaf extract on TRP1 / TRP2 / Tyr mRNA expression and tyrosinase activity inhibition. Quantitative real-time PCR showed that banana leaf extract decreased mRNA level of TRP1, TRP2 and Tyr gene and tyrosinase activity inhibition assay also revealed that banana leaf extract 65% decreased melanin production in B16F10 cell. Banana leaf extract cream can whiten the skin darkness induced by ultraviolet. Therefore, we successfully identified the whitening effect of banana leaf extract, and this finding suggested the banana leaf extract is a considerable potent cosmetic ingredient for skin whitening. Based on this, we anticipated further researches about banana leaf extract for mechanism to develop not only cosmetics but healthcare food or medicines.
Antiaging Effects of Musa sapientum L. (Banana) Leaf Extract
Dae Sung Yoo(유대성),Su Jin Jang(장수진),Yun Jeong Park(박윤정),Seung Hoi Kim(김승회),Hyung-seo Hwang(황형서) 한국생물공학회 2016 KSBB Journal Vol.31 No.2
To examine the possibility of using Musa sapientum L. (Banana) leaf extract as a cosmetic raw material, banana leaves grown in Jeju Island were extracted with 70% ethanol. Polysaccharides present in banana leaf extract were discarded by precipitation with cold ethanol. Polysaccharidediscarded banana leaf extract promoted procollagen and COL1A1 gene expression, but inhibited matrix metalloproteinase (MMP)-1 and MMP-2 gene expression in human skin fibroblasts when examined by real-time reverse-transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The active compound in banana leaves was identified by fractionation with various solvents. The chloroform fraction showed the highest anti-wrinkle efficacy and the active compound of chloroform fraction was identified as corosolic acid by NMR, FT-IR, EA, and HPLCMS. In addition, banana leaf extract showed anti-oxidative efficacy with an IC50 value of 67.91 ppm, as determined by DPPH free radical scavenging assay. Finally, the anti-wrinkle efficacy of banana leaf extract-containing cream was confirmed by clinical tests. Based on these results, banana leaves could have an application as a cosmetic raw material with anti-wrinkle efficacy.
Antiaging Effects of Musa sapientum L. (Banana) Leaf Extract
유대성,장수진,박윤정,김승회,황형서,Yoo, Dae Sung,Jang, Su Jin,Park, Yun Jeong,Kim, Seung Hoi,Hwang, Hyung-seo The Korean Society for Biotechnology and Bioengine 2016 KSBB Journal Vol.6 No.2
To examine the possibility of using Musa sapientum L. (Banana) leaf extract as a cosmetic raw material, banana leaves grown in Jeju Island were extracted with 70% ethanol. Polysaccharides present in banana leaf extract were discarded by precipitation with cold ethanol. Polysaccharide-discarded banana leaf extract promoted procollagen and COL1A1 gene expression, but inhibited matrix metalloproteinase (MMP)-1 and MMP-2 gene expression in human skin fibroblasts when examined by real-time reverse-transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The active compound in banana leaves was identified by fractionation with various solvents. The chloroform fraction showed the highest anti-wrinkle efficacy and the active compound of chloroform fraction was identified as corosolic acid by NMR, FT-IR, EA, and HPLC-MS. In addition, banana leaf extract showed anti-oxidative efficacy with an IC50 value of 67.91 ppm, as determined by DPPH free radical scavenging assay. Finally, the anti-wrinkle efficacy of banana leaf extract-containing cream was confirmed by clinical tests. Based on these results, banana leaves could have an application as a cosmetic raw material with anti-wrinkle efficacy.
논문 : 초음파 나노표면개질 공정기술에 의한 AISI304 스테인리스강의 표면나노구조화 및 압축잔류응력 형성
조인식 ( In Shik Cho ),동계령 ( Ji Ling Dong ),유대황 ( Dae Hwang Yoo ),서정화 ( Jung Hwa Suh ),아마노프 ( Auezhan Amanov ),신기삼 ( Kee Sam Shin ),이창순 ( Chang Soon Lee ),편영식 ( Young Shik Pyoun ),박인규 ( In Gyu Park ) 대한금속재료학회 ( 구 대한금속학회 ) 2010 대한금속·재료학회지 Vol.48 No.9
In this paper, the Ultrasonic Nanocrystalline Surface Modification (UNSM) surface treatment process was used to induce compressive residual stress and nanocrystalline structure by severe plastic deformation on the UNSM-treated surface. The test results for AISI304 stainless steel demonstrated that the grain size was found to be 23 nm, the dislocation density was increased by 0.2085×10(18) m-2, and the volume fraction of martensite is defined as 27.6% from austenite so that the surface hardness of the surface is increased from 200 Hv up to 515 Hv. The initial tensile residual stress is changed from 300 MPa to a compressive residual stress of 500 MPa after UNSM treatment. In addition, UNSM was applied under five various conditions, and the results of those conditions were defined as a function of depth quantitative.
논문: 건설용 이형 철근(SD35, SD30A)의 점용접성
이수연 ( Su Yeon Lee ),이찬규 ( Chan Gyu Lee ),( Vladimir S. Gornakov ),( Osamu Taguchi ),유대황 ( Dea Hwang Yoo ),윤국태 ( Kuk Tea Youn ),박대영 ( Dae Young Park ),허석환 ( Seok Hwan Huh ) 한국열처리공학회 2011 熱處理工學會誌 Vol.24 No.5
The spot weldability of metal joints between SD35 and SD30A steel was investigated. This study was aimed to determine the spot welding parameters (i.e. welding current and time) for reinforcement bar joint and to evaluate the weldability, mechanical strength, hardness and microstructure. The results indicated that shear strength and elongation of the welding joints at 12.5kA and 1000ms were 26.7kgf/mm2 and 22%, respectively. And fracture distribution of them by using shear and tensile test showed 100% necking mode and 100% ductile mode, respectively.
Binding Energy of Oxigen and Methane Adsorbed on Bundles of Open-Ended Single-Wall Carbon Nanotubes
서주연,유대황,곽진성,황윤회,김형국 부산대학교 유전체물성연구소 2004 유전체 논문집 Vol.3 No.
The adsorption of oxygen and methane on the bundles of open-ended single wall carbon nanotube(SWNT) was studied using the volumetric adsorption qst was obtained from the adsorption isotherm measurements performed at different temperatures. The trend in the values of the methane isosteric heat of adsorption showed three regions, representative of the adsorption on the different types of adsorption sites. In case of oxygen, on the other hand, such distinguishable regions were not appeared in the N-qst graph. From the results of qst the binding energies of oxygen and methane adsorbed on open-ended SWITs were estimated.