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백서 심근에서 plasmid 벡터를 이용한 유전자 전달 및 VEGF 유전자 발현의 특성
정진옥 ( Jin Ok Jeong ),박선진 ( Sun Jin Park ),허정은 ( Jeong Eun Huh ),정은아 ( Eun Ah Jung ),오주현 ( Ju Hyeon Oh ),권현철 ( Hyeon Cheol Gwon ),이영주 ( Young Joo Lee ),김선영 ( Sun Young Kim ),박정의 ( Jeong Euy Park ),이원로 대한내과학회 2001 대한내과학회지 Vol.60 No.1
N/A Background : The purpose of this study was to compare gene expression among newly designed eukaryotic expression vectors, and to characterize the pattern of vascular endothelial growth factor(VEGF) expression using the most potent plasmids DNA vector. Methods : After exposure of a beating rat heart (Sprague-Dawley, 250-300g), 5 different types of plasmid DNA was injected directly into the myocardium. Reporter protein was analyzed by ELISA in the extracted heart. Results : The vector harboring cytomegalovirus (CMV) promoter and enhancer induced the strongest expression of reporter gene (chloramphenicol acetyl transferase; CAT) compared to those of pC3.1, pEF1a, RSV, pActin in the rat heart via direct injection of plasmid DNA into the apex (p<0.001). Using pCN-CAT, gene expression showed a dose-dependent response over a range of 0.3-10 ㎍. CAT expression could be detected up to 30 days after 10 ㎍ of pCN-CAT injection with the maximal expression on day 5. In X-gal staining of injected pCN-lacZ gene, β-galactosidase was found only around the needle track in the apex. The expressed hVEGF121 had biologic activity with vascular permeability assay (Miles assay) in guinea pigs. After injection of pCN-hVEGF121 into the apex of the rat heart, the expression of VEGF protein was dose-dependent over the range of 25 and 500 ㎍. VEGF expression was detected up to 14 days with its peak on day 2 after injection of 250 ㎍ of pCN-hVEGF121. When plasmid was injected into the apex of the rat heart, the expression of VEGF in the heart showed concentration gradient from the apex to the base. However, the expression of CAT was detected only in the apex. Conclusion : Plasmids vector with hCMV IE promoter/enhancer will provide clear advantages over other previously developed plasmids and the information regarding the behaviors of VEGF expression may be useful in angiogenic gene therapy of the heart.(Korean J Med 60:3-15, 2001)
피부근염에서 발생한 자연 기종적증과 피하기종:증례 보고 및 국외 보고 증례들의 임상상에 대한 분석
이창균,고재중,송영욱,임용성,오주현,고영회,윤구섭 대한내과학회 1998 대한내과학회지 Vol.55 No.1
Spontaneous pneumomediastnum is a very rare complication of systemic autoimmune diseases, The precise mechanism of pneumomediastinum in dermatomyositis is not well known. Pulmonary alveoli rupture secondary to interstitial pneumonitis or pulmonary infarctions consequent upon vasculitis are the suggested mechanisms, Among the idiopathic inflammatory myopathies, dermatomyositis and polymyositis show similar clinical manifestations except skin lesions. But pneumomediastinum occurs exclusively in cases with dermatomyositis, not in case with polymyositis. In a literature review, patients with dermatomyositis and pneumomediastinum had some characteristic features. As compared with dermatomyositis without pneumomediastinum, CK level was normal in about half and concomitance of interstitial lung disease and cutaneous vasculitis were very frequent. We experienced a case of dermatomyositis with spontaneous pneumomediastinum and subcutaneous emphysema, Thus we report that with a review of the literature and analysis of reported cases.